RESUMEN
Supernatants of spleen cell culture from small-tumor-bearing mice, large-tumor-bearing mice, and large-tumor resected mice were fractionated by Sephadex G-100. The biological activity on tumor growth of all fractions was tested in vivo. It was found that only one fraction (MW: 220-250 kD) from spleen cell supernatants of small-tumor-bearing mice or large-tumor resected mice enhanced tumor growth. In spleen cell supernatants from large-tumor-bearing mice, two fractions had enhancing activity (MW: 220-250 and 100-10 kD). Thus, the surgical resection of large tumor induced disappearance of enhancing activity from the fraction of lower molecular weight.
Asunto(s)
Adenocarcinoma/análisis , Neoplasias Mamarias Experimentales/análisis , Bazo/análisis , Células Tumorales Cultivadas , Animales , Tolerancia Inmunológica , Ratones , Ratones Endogámicos BALB CRESUMEN
The mechanism accounted to accomplish the silver staining "fast technique" on tissues sections was studied towards the correlation among histophotometric measures concerning the silver staining intensity and the intensity provided by some histochemical reactions performed on spleen and liver sections from rats and pigs. By treating previously these histological sections with thioglycolate or oxalate solutions in progressive concentrations and afterwards subjecting them to a silver staining "fast technique", it was demonstrated that the silver staining intensity decreases proportionally to the thioglycolate or the oxalate solution concentration. The regression line of the silver staining intensity on the thioglycolate or the oxalate solution concentration was established, as well as its regression coefficient. On the other hand, on histological sections previously subjected to the thioglycolate or the oxalate treatment, some histochemical techniques were performed and the histophotometric measures concerning the intensity of each histochemical technique used were taken and its regression line, as well as its regression coefficient were established. By comparing the silver staining intensity regression line (or its regression coefficient) with the regression lines established for the histochemical techniques used, it was tested the correlation between the silver staining and the reactivity of some reactive groups contained into the tissues. In this manner, the influence of such reactive groups on the silver staining development was tested. The results show that there is no correlation between the silver staining intensity and the reactivity degree of the reducing groups (-SH- and carbonyl group), the 1-2-glycolic group, as well as of some protein reactive groups (phenol, imidazole, carboxylic groups). On the other hand, taking into account the respective regression lines and its regression coefficient, the comparison between the silver staining intensity and the Prussian blue reaction intensity shows a close relationship between them. This finding suggests that for the silver staining "fast technique" the tissue bound ferric ion accomplishes a very important role and can be accounted for its mechanism and its histochemical meaning.
Asunto(s)
Histocitoquímica/métodos , Hígado/análisis , Plata , Bazo/análisis , Animales , Fotometría , Ratas , Análisis de Regresión , Coloración y Etiquetado , PorcinosRESUMEN
Se estudiaron 46 bazos humanos, donde se evidencia que: el tipo I de distribución extrabilial con dos ramas., (incluyendo arterias polares como ramas) 71.7% y del tipo II con tres ramas en el 28.2% de los órganos. La incidencia de polar superior en forma global, alcanzó el 19%, mientras la polar inferior alcanzó el 47.8% ya dentro de hilio esplénico, demostramos el tipo I en el 67.3% mientras el tipo II se demostró en el 32.6% de nuestro estudio. En la distribución segmentaria o parenquimatosa, nuestro estudio. En la distribución segmentaria o parenquimatosa, la presentación en cuatro segmentos fué la dominante con 54.3%, seguida por la de tres con 23.9% y finalmente la de cinco con 21.7%. Con el presente estudio se demuestran las observaciones sobre las características morfológicas de la circulación esplénica en nuestro medio, las cuales nos sirven de base fundamental para realizar la cirugía planificada y conservadora del bazo
Asunto(s)
Adulto , Humanos , Bazo/análisis , Bazo/cirugíaRESUMEN
The procedure for processing and identifying the victims of the Jonestown, Guyana tragedy is outlined. Autopsies were performed on seven of the victims, and the autopsy anatomic and toxicologic findings are presented. Problems encountered in the interpretation of the autopsy findings are noted.