RESUMEN
Fowl Adenoviruses (FAdVs) are widely-distributed pathogens across the globe. Fowl adenovirus serotype 4 (FAdV-4), the causative agent for chicken hydropericardium syndrome (HPS) with a high mortality in the infected flocks, has caused severe economic losses to the poultry industry of China in the past few years. Although vaccination against FAdV-4â¯has been implemented, the prevention and control of FAdV-4 infection is still not very successful. Here, we report that FAdV-4 markedly inhibits Leghorn male hepatoma (LMH) cell growth and this inhibition could be abolished by a small molecule SP600125, a JNK MAPK specific inhibitor. Furthermore, SP600125 considerably suppressed FAdV-4-induced phosphorylation of p38 and JNK MAPK. Importantly, SP600125 promoted type I interferon production associated with inhibition of FAdV-4 replication. Thus, FAdV-4 might employ the JNK MAPK pathway for the benefit of its replication, and SP600125 may have the potential of being used as an anti-virus drug for the control FAdV-4 infection.
Asunto(s)
Infecciones por Adenoviridae/veterinaria , Antracenos/farmacología , Aviadenovirus/efectos de los fármacos , Carcinoma Hepatocelular/veterinaria , Pollos/virología , Neoplasias Hepáticas/veterinaria , Enfermedades de las Aves de Corral/virología , Infecciones por Adenoviridae/virología , Animales , Aviadenovirus/inmunología , Aviadenovirus/fisiología , Carcinoma Hepatocelular/virología , Línea Celular Tumoral , Interferón Tipo I/metabolismo , Neoplasias Hepáticas/virología , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Masculino , Fosforilación/efectos de los fármacos , Serogrupo , Replicación Viral/efectos de los fármacosRESUMEN
Immunization against Angara disease virus (ADV), a serotype 4 avian adenovirus, and Newcastle disease virus (NDV), an avian paramyxovirus serotype 1, is the mainstay of a broiler vaccination programme, while polyether ionophores usually form an essential component of a broiler medication programme in most parts of India and Pakistan. The role of polyether ionophores in the protective immune responses of broiler chickens vaccinated and challenged with ADV and NDV was investigated. A total of 1600 birds were divided into eight groups of 200 birds each. First four groups were vaccinated against NDV and ADV, while the remaining four served as unvaccinated controls. The first 3 groups of birds were administered salinomycin, monensin and cyclophosphamide (CYP), respectively. The last group served as an untreated control. The same treatment schedule was also followed for the next four unvaccinated groups. The post-vaccination and post-challenge serological responses to NDV and ADV, body and lymphoid organ weight gains, post-challenge survival rate and detection of NDV and ADV in the tissues of infected birds were evaluated. Birds administered salinomycin showed a significant stimulation of protective immune responses against both NDV and ADV as compared to the untreated and CYP-treated birds. Monensin also enhanced the protective immune responses against both viruses but the effect was not statistically significant. Thus, it is concluded that monensin and salinomycin augment the anti-NDV and anti-ADV immune responses in broiler chickens, which supports their use in poultry flocks.
Asunto(s)
Infecciones por Adenoviridae/veterinaria , Aviadenovirus/inmunología , Pollos , Ionóforos/farmacología , Enfermedad de Newcastle/prevención & control , Enfermedades de las Aves de Corral/prevención & control , Infecciones por Adenoviridae/inmunología , Infecciones por Adenoviridae/prevención & control , Animales , Anticuerpos Antivirales/sangre , Aviadenovirus/efectos de los fármacos , Peso Corporal/efectos de los fármacos , Ciclofosfamida/administración & dosificación , Ciclofosfamida/farmacología , Factores Inmunológicos/administración & dosificación , Factores Inmunológicos/farmacología , Inmunosupresores/administración & dosificación , Inmunosupresores/farmacología , Ionóforos/administración & dosificación , Tejido Linfoide/efectos de los fármacos , Enfermedad de Newcastle/inmunología , Virus de la Enfermedad de Newcastle/efectos de los fármacos , Virus de la Enfermedad de Newcastle/inmunología , Enfermedades de las Aves de Corral/inmunología , Enfermedades de las Aves de Corral/virología , Distribución Aleatoria , Análisis de Supervivencia , Distribución Tisular , Vacunas Virales/administración & dosificaciónRESUMEN
In this study we show that a type I-IFN inducing compound, S-28828, modulated the pathogenesis of an avian type II adenovirus in turkeys. By itself, S-28828 induced a strong reaction in the spleen characterized by hyperplasia of the red and white pulps as well as an increase in lymphoid cell aggregations. Oral administration of S-28828 before the time of virus inoculation suppressed significantly (P<0.05) the replication of hemorrhagic enteritis virus (HEV) in turkeys. Two doses of 5 or 50 mg of S-28828 administered at 2 days before and at the day of virus inoculation inhibited HEV-induced pathological and histopathological lesions. Virus-induced apoptosis and reduced IgM-surface expression of B cells were suppressed by low dose S-28828 treatment. These results are of interest because mammalian adenoviruses were shown to be resistant to antiviral effects of type I IFN, the major effector cytokine induced by S-28828.
Asunto(s)
Infecciones por Adenoviridae/veterinaria , Aminoquinolinas/uso terapéutico , Aviadenovirus/efectos de los fármacos , Inductores de Interferón/uso terapéutico , Enfermedades de las Aves de Corral/tratamiento farmacológico , Infecciones por Adenoviridae/tratamiento farmacológico , Infecciones por Adenoviridae/virología , Animales , Aviadenovirus/patogenicidad , Aviadenovirus/fisiología , Enteritis/tratamiento farmacológico , Enteritis/veterinaria , Enteritis/virología , Hemorragia Gastrointestinal/tratamiento farmacológico , Hemorragia Gastrointestinal/veterinaria , Hemorragia Gastrointestinal/virología , Enfermedades de las Aves de Corral/virología , Pavos , Virulencia , Replicación Viral/efectos de los fármacosRESUMEN
Using the chloroquine-modified calcium phosphate coprecipitation technique, fowl adenovirus serotype 1 (FAV 1) DNA transfects efficiently chicken cell cultures. Infection of FAV 1 DNA transfected cells with helper dependent avian adeno-associated virus (AAAV) results in the production of AAAV progeny, being detected by an indirect immunofluorescence assay. These findings indicate that FAV 1 DNA introduced into the host cell promotes actively the growth of AAAV.
Asunto(s)
Aviadenovirus/crecimiento & desarrollo , Infecciones por Adenoviridae/diagnóstico , Infecciones por Adenoviridae/tratamiento farmacológico , Infecciones por Adenoviridae/veterinaria , Animales , Aviadenovirus/efectos de los fármacos , Aviadenovirus/genética , Pollos , Cloroquina/farmacología , ADN Viral/análisis , Técnica del Anticuerpo Fluorescente , Enfermedades de las Aves de Corral/diagnóstico , Enfermedades de las Aves de Corral/tratamiento farmacológico , Transfección , Replicación ViralRESUMEN
Effect of formaldehyde liberated from formalin on disinfection of the surface of a filtered air under positive pressure (FAPP) house and the surface of equipment placed in the house was studied. Escherichia coli, Staphylococcus aureus and avian adenovirus were used in this study as a indicator to evaluate degree of disinfection. Satisfactory degree of disinfection of the house and equipment was obtained when formaldehyde was liberated from 40 ml. of formalin per 1 m.3 capacity. Bacteria and virus used in the experiments, as well as air-borne organisms in the house, were perfectly inactivated by fumigation for 24 hours at the level of formalin mentioned above.
Asunto(s)
Microbiología del Aire , Desinfección/métodos , Formaldehído/farmacología , Vivienda para Animales , Esterilización/métodos , Animales , Aviadenovirus/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Filtración , Presión , Staphylococcus aureus/efectos de los fármacosRESUMEN
Studied were some physico-chemical properties of four CELO virus strains isolated in Bulgaria. Their behaviour to ether, chloroform and 25 per cent trypsin, and their state at 56 degrees C and at pH 3, 0 were determined through their infectious titer as obtained on 9--10-day old chicken embryos. It was found that the size of the investigated viruses is within the range of 50 to 100 mmu, they belong to the DNA-possessing group, are resistant at 56 degrees C and at pH EQUALS 3,0. They are not sensitive to ether, chloroform and trypsin. Their physico-chemical properties are fully comparable with those of the avian adenoviruses-CELO.