RESUMEN
The current study was conducted to examine the point prevalence of gastrointestinal parasites of migratory quails. Due to its economic importance, the control of ascaridiosis is critical. Migration of birds is considered to enhance the global spread and cross-species transmission of pathogens. The current study was aimed to detect A.galli in migratory quails, a potential contributory risk factor for transmission of this parasite to local birds. A total of 230 migratory quails were trapped using nets from migratory routes in Balochistan and examined under the compound microscope for the presence of A. galli. Conventionally, A. galli was identified by its morphology with the presence of three large lips and absence of posterior esophageal bulb. Results revealed that out of 230, 120 (52.17%) quails were positive for A. galli by targeting COX1 gene (533 bp) by using conventional PCR. Further, the amplicon was sequenced which showed 99% similarity with A. galli publically available in NCBI Gen Bank. Phylogenetic analysis of sequences of our isolated parasite indicated the close relationship with A.galli isolated from chickens. In conclusion migratory quails and other migratory birds may play a key role in spreading and transmission of these parasites and other pathogens to domestic chicken. Therefore, strict biosecurity measures should be adopted especially for commercial poultry farms.
O presente estudo foi conduzido para examinar a prevalência pontual de parasitas gastrointestinais de codornas migratórias. Devido à sua importância econômica, o controle da ascaridiose é fundamental. Considera-se que a migração de aves aumenta a disseminação global e a transmissão entre espécies de patógenos. O presente estudo teve como objetivo detectar A. galli em codornas migratórias, um potencial fator de risco contributivo para a transmissão desse parasita para aves locais. Um total de 230 codornas migratórias foi capturado, usando redes de rotas migratórias no Baluchistão e examinadas sob o microscópio composto para a presença de A. galli. Convencionalmente, o A. galli foi identificado por sua morfologia com a presença de três grandes lábios e ausência de bulbo esofágico posterior. Os resultados revelaram que de 230, 120 (52,17%) codornas foram positivas para A. galli por direcionamento do gene COX1 (533 pb) usando PCR convencional. Além disso, o amplicon foi sequenciado, que mostrou 99% de similaridade com A. galli publicamente disponível no NCBI Gen Bank. A análise filogenética das sequências do nosso parasita isolado indicou a estreita relação com A. galli isolado de galinhas. Em conclusão, codornas migratórias e outras aves migratórias podem desempenhar papel fundamental na disseminação e transmissão desses parasitas e outros patógenos para as galinhas domésticas. Portanto, medidas rigorosas de biossegurança devem ser adotadas, especialmente para granjas comerciais.
Asunto(s)
Animales , Ascaridia/anatomía & histología , Coturnix/parasitología , Conformación Molecular , PakistánRESUMEN
Encapsulated third stage nematode larvae collected from the abdominal cavity of the Trinidadian hylid frog Hyla minuta are referred to the rare anisakid genus Brevimulticaecum. These are the first specimens of Brevimulticaecum from a tropical frog, their presence may reflect the importance of amphibians as intermediate or paratenic hosts of reptilian parasites. (AU)
Asunto(s)
21003 , Anuros/parasitología , Ascaridia/aislamiento & purificación , Ascaridia/anatomía & histología , Ascaridia/clasificación , Ascaridia/parasitología , Trinidad y TobagoRESUMEN
Thynnascaris reliquens sp. n. is described from the type host, the sheepshead, Archosargus probatocephalus (Walbaum), and other fishes of the northern Gulf of Mexico and southern Florida, including the Gulf toadfish, Opsanus beta (Goode and Bean); the spiny boxfish, Chilomycterus schoepfi (Walbaum); the slippery dick, Halichoeres bivittatus (Bloch); and the Atlantic croaker, Micropogon undulatus (Linnaeus). Its salient characters are: length up to approximately to 12.5 cm; lips broad, with pedunculate pulp and equatorially constricted flanges; esophagus 8 to 12% and spicles 3 to 6% of body length; preanal and postanal papillae up to 33 and 6 pairs, respectively. In the type host, some adults attained a considerably greater size than in other hosts, and their labial morphology changed as the maximum length was approached. Thynnascaris habena is redescribed from the oyster toadfish, Opsanus tau (Linnaeus), its type host, on the basis of examination of specimens collected from Massachusetts, Virginia, and Georgia. Thynnascaris melichthysi (Olsen, 1952) and T. ogcocephali (Olsen, 1952) are transferred from the genus Contracaecum as new combinations.