Asunto(s)
Corticoesteroides/uso terapéutico , Albendazol/uso terapéutico , Antihelmínticos/uso terapéutico , Aracnoides/parasitología , Neurocisticercosis/tratamiento farmacológico , Premedicación , Adulto , Antígenos Helmínticos/líquido cefalorraquídeo , Proteínas del Líquido Cefalorraquídeo/líquido cefalorraquídeo , Relación Dosis-Respuesta a Droga , Esquema de Medicación , Quimioterapia Combinada , Femenino , Glucosa/líquido cefalorraquídeo , Humanos , Masculino , Persona de Mediana Edad , Neurocisticercosis/líquido cefalorraquídeo , Resultado del TratamientoRESUMEN
Trypanosoma cruzi and Toxoplasma gondii are protozoan parasites capable of causing infections of the nervous system. In order to determine effects of infection by these organisms on intercellular communication in the brain, dye coupling and connexin abundance and distribution were examined in leptomeningeal cells and astrocytes infected with T. cruzi or T. gondii. For both cell types infected with either type of protozoan parasite, intercellular diffusion of intracellularly injected Lucifer Yellow was dramatically reduced. Immunocytochemistry with antibodies specific for connexin43 (in astrocytes) or both connexin43 and connexin26 (for leptomeningeal cells) demonstrated that punctate gap junctional staining was much reduced in infected cells, although uninfected neighbors could display normal connexin abundance and distribution. Western blot analyses revealed that connexin43 abundance in both cell types infected with either parasite was similar to that in uninfected cells. Phosphorylation state of connexin43 (inferred from electrophoretic mobility of connexin43 isoforms) was not significantly affected by the infection process. Immunocytochemistry of whole brains from animals acutely infected with either parasite also showed a marked reduction in connexin43 expression. We conclude that infection of both types of brain cells with either protozoan parasite results in a loss of intercellular communication and organized gap junction plaques without affecting expression levels or posttranslational processing of gap junction proteins. Presumably, these changes in gap junction distribution result from altered targeting of the junctional protein to the plasma membrane, and/or from changes in assembly of subunits into functional channels.
Asunto(s)
Astrocitos/parasitología , Enfermedad de Chagas/fisiopatología , Uniones Comunicantes/fisiología , Toxoplasmosis Animal/fisiopatología , Animales , Aracnoides/citología , Aracnoides/parasitología , Astrocitos/química , Astrocitos/ultraestructura , Western Blotting , Comunicación Celular/fisiología , Corteza Cerebral/química , Corteza Cerebral/citología , Corteza Cerebral/parasitología , Conexina 26 , Conexina 43/análisis , Conexinas/análisis , Técnica del Anticuerpo Fluorescente , Colorantes Fluorescentes/farmacocinética , Uniones Comunicantes/química , Isoquinolinas/farmacocinética , Piamadre/citología , Piamadre/parasitología , Ratas , Ratas Sprague-DawleyAsunto(s)
Encefalopatías/parasitología , Infecciones por Cestodos/parasitología , Adulto , Animales , Aracnoides/parasitología , Encefalopatías/diagnóstico por imagen , Cestodos/patogenicidad , Infecciones por Cestodos/diagnóstico por imagen , Nervios Craneales/parasitología , Humanos , Masculino , Tomografía Computarizada por Rayos XRESUMEN
Cysticercosis, a chronic helminthic disease rarely diagnosed during the patients' life is found in the practice of pathoanatomists. The paper presents two observations in one of which cystic and branching forms of the disease were combined.