Asunto(s)
Animales , Ratones , Clofazimina/síntesis química , Clofazimina/uso terapéutico , Itraconazol/síntesis química , Itraconazol/uso terapéutico , Micosis/clasificación , Micosis/fisiopatología , Paracoccidioidomicosis/diagnóstico , Paracoccidioidomicosis/fisiopatología , Paracoccidioidomicosis/rehabilitación , Anticuerpos Antifúngicos/fisiología , Anticuerpos Antifúngicos/inmunología , Ratones Endogámicos BALB C/fisiología , Ratones Endogámicos BALB C/genéticaRESUMEN
In this paper we relate that sera from paracoccidioidomycosis patients inhibited the mitogen-induced proliferative responses of normal mononuclear cells. Treatment of these sera with 2.5% polyethyleneglycol (PEG), a method classically used to precipitate immune complexes, significantly reduced their inhibitory activity. Immunoblot analysis of the PEG precipitates identified a 34-kDa polypeptide, recognized by rabbit anti-P. brasiliensis IgG. Patient mononuclear cells showed partial restoration of their proliferative capacity after 24 h culture in medium alone, which suggests release of membrane-bound molecules in the culture medium. These findings indicate that circulating P. brasiliensis antigens, complexed or not with antibodies, may play a negative immunoregulatory effect in the mitogen-induced proliferative responses of paracoccidioidomycosis patients.