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1.
Food Res Int ; 194: 114888, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-39232522

RESUMEN

Alcoholic fermentation is one of man's most efficient food preservation processes, and innovations in this area are a trend in food science and nutrition. In addition to the classic Saccharomyces yeasts, various other species may have desirable characteristics for obtaining fruit wines. This study investigated the profile of non-Saccharomyces commercial yeasts compared with S. cerevisiae regarding pineapple wine's chemical composition and bioaccessibility. The fermentation profile of the yeasts Lachancea thermotolerans, Brettanomyces bruxellensis, Brettanomyces lambicus, and S. cerevisiae was evaluated for sugar and alcohol content, and the pineapple wines obtained were analyzed for amino acids, phenolics, and organic acids by HPLC and volatile profile by GC/MS. All yeast strains were able to produce ethanol and glycerol at acceptable levels. L. thermotolerans produced higher levels of lactic acid (0.95 g/L) and higher consumption of free amino acids. B. bruxellensis produced higher levels of individual phenolics and ethanol 109 g/L. The alcoholic fermentation process improved the bioaccessibility of phenolics such as catechin (237 %), epigallocatechin gallate (81 %), procyanidin B1 (61 %) and procyanidin B2 (61 %). The yeasts differed in their volatile profiles, with Brettanomyces and Lachancea producing higher levels of compounds associated with pineapple aroma, such as ester ethyl butyrate (260-270 µg/L). These results demonstrate the importance of choosing the yeast strain for the conduction of alcoholic fermentation and that the yeasts Brettanomyces and Lachancea showed technological potential in obtaining pineapple wines. This study contributes to developing processes for obtaining fruit wines by highlighting two non-Saccharomyces yeast species with technological potential for alcoholic fermentations.


Asunto(s)
Ananas , Etanol , Fermentación , Saccharomyces cerevisiae , Vino , Vino/análisis , Ananas/química , Saccharomyces cerevisiae/metabolismo , Etanol/metabolismo , Etanol/análisis , Fenoles/análisis , Fenoles/metabolismo , Aminoácidos/análisis , Aminoácidos/metabolismo , Brettanomyces/metabolismo , Saccharomycetales/metabolismo , Compuestos Orgánicos Volátiles/análisis , Cromatografía de Gases y Espectrometría de Masas , Frutas/química
2.
Toxins (Basel) ; 16(8)2024 Aug 07.
Artículo en Inglés | MEDLINE | ID: mdl-39195754

RESUMEN

Pineapple Fruitlet Core Rot (FCR) is a fungal disease characterized by a multi-pathogen pathosystem. Recently, Fusarium proliferatum, Fusarium oxysporum, and Talaromyces stollii joined the set of FCR pathogens until then exclusively attributed to Fusarium ananatum. The particularity of FCR relies on the presence of healthy and diseased fruitlets within the same infructescence. The mycobiomes associated with these two types of tissues suggested that disease occurrence might be triggered by or linked to an ecological chemical communication-promoting pathogen(s) development within the fungal community. Interactions between the four recently identified pathogens were deciphered by in vitro pairwise co-culture bioassays. Both fungal growth and mycotoxin production patterns were monitored for 10 days. Results evidenced that Talaromyces stollii was the main fungal antagonist of Fusarium species, reducing by 22% the growth of Fusarium proliferatum. A collapse of beauvericin content was observed when FCR pathogens were cross-challenged while fumonisin concentrations were increased by up to 7-fold. Antagonism between Fusarium species and Talaromyces stollii was supported by the diffusion of a red pigmentation and droplets of red exudate at the mycelium surface. This study revealed that secondary metabolites could shape the fungal pathogenic community of a pineapple fruitlet and contribute to virulence promoting FCR establishment.


Asunto(s)
Ananas , Fusarium , Micotoxinas , Enfermedades de las Plantas , Talaromyces , Ananas/microbiología , Fusarium/crecimiento & desarrollo , Fusarium/metabolismo , Fusarium/patogenicidad , Talaromyces/crecimiento & desarrollo , Talaromyces/metabolismo , Enfermedades de las Plantas/microbiología , Micotoxinas/metabolismo , Frutas/microbiología , Técnicas de Cocultivo
3.
Sci Rep ; 14(1): 17194, 2024 07 26.
Artículo en Inglés | MEDLINE | ID: mdl-39060260

RESUMEN

The production of pineapple generates significant quantities of harvest and processing residues, which are very little used. This study evaluates compost quality using pineapple residues and poultry litter. Five composting treatments were tested, varying following proportions of crown, pineapple processing wastes (PPW), pineapple harvest residue (PHR), and poultry litter (PL). Various parameters were analyzed, including pH, electrical conductivity, CO2 evolution rate, water content, organic carbon, nitrogen compounds, phosphorus, potassium, calcium, magnesium, copper, and zinc. Additionally, the perceptions of producers and processors regarding compost quality were gathered. Results indicated that microbial decomposition increased temperature, pH, CO2 release, and nitrogen content while reducing electrical conductivity and organic carbon. Composts demonstrated favorable characteristics for crop fertilization, with C4 (75% PHR + 25% PL) compost showing the best chemical properties. Producers and processors preferred the color, odor, and structure of C4 (75% PHR + 25% PL) and C5 (56.25% crown + 18.75% PPW + 25% PL) composts. Overall, composting pineapple residues with poultry litter yields composts suitable for plant fertilization, particularly C4 and C5 formulations, offering potential for sustainable waste valorization in agriculture.


Asunto(s)
Ananas , Compostaje , Aves de Corral , Animales , Compostaje/métodos , Concentración de Iones de Hidrógeno , Nitrógeno/análisis , Suelo/química , Estiércol/análisis , Fertilizantes/análisis
4.
Nutrients ; 16(13)2024 Jun 28.
Artículo en Inglés | MEDLINE | ID: mdl-38999808

RESUMEN

Bromelain is a mixture of proteolytic enzymes primarily extracted from the fruit and stem of the pineapple plant (Ananas comosus). It has a long history of traditional medicinal use in various cultures, particularly in Central and South America, where pineapple is native. This systematic review will delve into the history, structure, chemical properties, and medical indications of bromelain. Bromelain was first isolated and described in the late 19th century by researchers in Europe, who identified its proteolytic properties. Since then, bromelain has gained recognition in both traditional and modern medicine for its potential therapeutic effects.


Asunto(s)
Ananas , Bromelaínas , Bromelaínas/uso terapéutico , Bromelaínas/farmacología , Humanos , Ananas/química , Animales , Frutas/química
5.
Viruses ; 16(7)2024 Jul 16.
Artículo en Inglés | MEDLINE | ID: mdl-39066307

RESUMEN

The cultivation of pineapple (Ananas comosus) is threatened worldwide by mealybug wilt disease of pineapple (MWP), whose etiology is not yet fully elucidated. In this study, we characterized pineapple mealybug wilt-associated ampeloviruses (PMWaVs, family Closteroviridae) from a diseased pineapple plant collected from Reunion Island, using a high-throughput sequencing approach combining Illumina short reads and Nanopore long reads. Reads co-assembly resulted in complete or near-complete genomes for six distinct ampeloviruses, including the first complete genome of pineapple mealybug wilt-associated virus 5 (PMWaV5) and that of a new species tentatively named pineapple mealybug wilt-associated virus 7 (PMWaV7). Short reads data provided high genome coverage and sequencing depths for all six viral genomes, contrary to long reads data. The 5' and 3' ends of the genome for most of the six ampeloviruses could be recovered from long reads, providing an alternative to RACE-PCRs. Phylogenetic analyses did not unveil any geographic structuring of the diversity of PMWaV1, PMWaV2 and PMWaV3 isolates, supporting the current hypothesis that PMWaVs were mainly spread by human activity and vegetative propagation.


Asunto(s)
Ananas , Closteroviridae , Genoma Viral , Secuenciación de Nucleótidos de Alto Rendimiento , Filogenia , Enfermedades de las Plantas , Ananas/virología , Enfermedades de las Plantas/virología , Closteroviridae/genética , Closteroviridae/clasificación , Closteroviridae/aislamiento & purificación , Reunión , ARN Viral/genética
6.
Neotrop Entomol ; 53(4): 1002-1008, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38833136

RESUMEN

This is the first record of Melanoloma viatrix Hendel as well as in its host (pineapple) in Brazil. Previously, M. viatrix had been found in pineapple plantations in Peru, Colombia, Venezuela, and Ecuador. In Brazil, this fly was initially reported in pineapple purchased from a commercial establishment in the municipality of Porto Grande, Amapá State, in 2020. Subsequently, M. viatrix was found in a commercial pineapple plantation, Ananas comosus (L.) Merr. cv. Pérola, also in Porto Grande, Amapá State. In the pineapple samples, 2,320 specimens of M. viatrix were collected on a single day on that rural property (approximately 2 ha). The larvae open galleries in the pulp that cause the pineapple to rot, making it unviable for consumption and commercial sale. This communication aims to alert phytosanitary authorities of the need to establish control strategies to prevent the spread of the pineapple fly to other states in Brazil.


Asunto(s)
Ananas , Dípteros , Larva , Brasil , Animales
7.
Int J Biol Macromol ; 273(Pt 2): 133089, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38878936

RESUMEN

This review shows the endeavors performed to prepare immobilized formulations of bromelain extract, usually from pineapple, and their use in diverse applications. This extract has a potent proteolytic component that is based on thiol proteases, which differ depending on the location on the fruit. Stem and fruit are the areas where higher activity is found. The edible origin of this enzyme is one of the features that determines the applications of the immobilized bromelain to a more significant degree. The enzyme has been immobilized on a wide diversity of supports via different strategies (covalent bonds, ion exchange), and also forming ex novo solids (nanoflowers, CLEAs, trapping in alginate beads, etc.). The use of preexisting nanoparticles as immobilization supports is relevant, as this facilitates one of the main applications of the immobilized enzyme, in therapeutic applications (as wound dressing and healing components, antibacterial or anticancer, mucus mobility control, etc.). A curiosity is the immobilization of this enzyme on spores of probiotic microorganisms via adsorption, in order to have a perfect in vivo compatibility. Other outstanding applications of the immobilized enzyme are in the stabilization of wine versus haze during storage, mainly when immobilized on chitosan. Curiously, the immobilized bromelain has been scarcely applied in the production of bioactive peptides.


Asunto(s)
Bromelaínas , Enzimas Inmovilizadas , Bromelaínas/química , Bromelaínas/metabolismo , Enzimas Inmovilizadas/química , Enzimas Inmovilizadas/metabolismo , Ananas/enzimología , Ananas/química , Nanopartículas/química
8.
Food Chem ; 455: 139956, 2024 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-38843713

RESUMEN

Pineapple aroma is one of the most important sensory quality traits that influences consumer purchasing patterns. Reported in this paper is a high throughput method to quantify in a single analysis the key volatile organic compounds that contribute to the aroma of pineapple cultivars grown in Australia. The method constituted stable isotope dilution analysis in conjunction with headspace solid-phase microextraction coupled with gas-chromatography mass spectrometry. Deuterium labelled analogues of the target analytes purchased commercially were used as internal standards. Twenty-six volatile organic compounds were targeted for quantification and the resulting calibration functions of the matrix -matched validated method had determination coefficients (R2) ranging from 0.9772 to 0.9999. The method was applied to identify the key aroma volatile compounds produced by popular pineapple cultivars such as 'Aus Carnival', 'Aus Festival', 'Aus Jubilee', 'Aus Smooth (Smooth Cayenne)' and 'Aussie Gold (73-50)', grown in Queensland, Australia. Pineapple cultivars varied in its content and composition of free volatile components, which were predominantly comprised of esters, followed by terpenes, alcohols, aldehydes, and ketones.


Asunto(s)
Ananas , Cromatografía de Gases y Espectrometría de Masas , Odorantes , Microextracción en Fase Sólida , Compuestos Orgánicos Volátiles , Ananas/química , Compuestos Orgánicos Volátiles/química , Compuestos Orgánicos Volátiles/análisis , Australia , Odorantes/análisis , Técnicas de Dilución del Indicador
9.
Compr Rev Food Sci Food Saf ; 23(3): e13359, 2024 05.
Artículo en Inglés | MEDLINE | ID: mdl-38720571

RESUMEN

The recent increase in the harvesting and industrial processing of tropical fruits such as pineapple and papaya is leading to unavoidable amounts of byproducts rich in valuable compounds. Given the significance of the chemical composition of these byproducts, new research avenues are opening up to exploit them in the food industry. In this sense, the revalorization of pineapple and papaya byproducts is an emerging trend that is encouraging the full harnessing of these tropical fruits, offering the opportunity for developing innovative value-added products. Therefore, the main aim of this review is to provide an overview of the state of the art of the current valorization applications of pineapple and papaya byproducts in the field of food industry. For that proposal, comprehensive research of valorization applications developed in the last years has been conducted using scientific databases, databases, digital libraries, and scientific search engines. The latest valorization applications of pineapple and papaya byproducts in the food industry have been systematically revised and gathered with the objective of synthesizing and critically analyzing existing scientific literature in order to contribute to the advancement of knowledge in the field of tropical byproduct revalorization providing a solid foundation for further research and highlighting scientific gaps and new challenges that should be addressed in the future.


Asunto(s)
Ananas , Carica , Frutas , Carica/química , Ananas/química , Frutas/química , Industria de Alimentos , Manipulación de Alimentos/métodos
10.
Food Chem ; 451: 139440, 2024 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-38692245

RESUMEN

The preservation of fresh-cut fruits and vegetables has attracted attention to the shelf-life reduction caused by high humidity. Herein, alginate/copper ions cross-linking, in-situ growth and self-assembly techniques of metal-organic frameworks (MOFs) were utilized to prepare a moisture responsive hydrogel bead (HKUST-1@ALG). As the multistage porous structure formation, tea tree essential oil (TTO) load capacity in hydrogel bead (TTO-HKUST-1@ALG) was increased from 6.1% to 21.6%. TTO-HKUST-1@ALG had excellent moisture response performance, and the release rates of TTO increased from 33.89% to 70.98% with moisture increasing from 45% to 95%. Besides, TTO-HKUST-1@ALG exhibited excellent antimicrobial, antioxidant capacity, and biocompatibility. During storage, TTO-HKUST-1@ALG effectively improved the cell membrane integrity by maintaining the balance of reactive oxygen species metabolism. The degradation of cell wall structure and tissue softening were delayed by inhibiting the cell wall-degrading enzymes activity. Briefly, TTO-HKUST-1@ALG improved the storage quality and extended shelf-life of fresh-cut pineapple, which was a promising preservative.


Asunto(s)
Ananas , Conservación de Alimentos , Hidrogeles , Estructuras Metalorgánicas , Aceites Volátiles , Ananas/química , Aceites Volátiles/química , Hidrogeles/química , Estructuras Metalorgánicas/química , Conservación de Alimentos/métodos , Conservación de Alimentos/instrumentación , Frutas/química , Antioxidantes/química , Conservantes de Alimentos/farmacología , Conservantes de Alimentos/química
11.
Food Chem ; 451: 139454, 2024 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-38703725

RESUMEN

Morphology regulation of heterodimer nanoparticles and the use of their asymmetric features for further practical applications are crucial because of the rich optical properties and various combinations of heterodimers. This work used silicon to asymmetrically wrap half of a gold sphere and grew gold branches on the bare gold surface to form heterogeneous nano pineapples (NPPs) which can effectively improve Surface-enhanced Raman scattering (SERS) properties through chemical enhancement and lightning-rod effect respectively. The asymmetric structures of NPPs enabled them to self-assemble into the monolayer membrane with consistent branch orientation. The prepared substrate had high homogeneity and better SERS ability than disorganized substrates, and achieved reliable detection of malachite green (MG) in clams with a detection limit of 7.8 × 10-11 M. This work provided a guide to further revise the morphology of heterodimers and a new idea for the use of asymmetric dimers for practically photochemical and biomedical sensing.


Asunto(s)
Oro , Colorantes de Rosanilina , Silicio , Espectrometría Raman , Colorantes de Rosanilina/química , Espectrometría Raman/métodos , Oro/química , Silicio/química , Animales , Ananas/química , Nanopartículas del Metal/química , Bivalvos/química , Límite de Detección , Propiedades de Superficie
12.
Int J Biol Macromol ; 270(Pt 1): 132299, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38735609

RESUMEN

Mulch films were fabricated from polylactic acid (PLA) with cellulose nanocrystals (PNC) extracted from pineapple leaves. The PNC was modified by incorporating 4 wt% triethoxyvinylsilane (TEVS), designated as 4PNC, to enhance its interaction with PLA. The films incorporated varying concentrations of PNC (1, 2, 4, and 8 wt%). The results indicated that higher PNC concentrations increased the water vapor permeability (WVP) and biodegradability of the composite films, while reducing light transmission. Films containing 4PNC, particularly at 4 wt% (PLA/4PNC-4), exhibited an 11.18 % increase in elongation at break compared to neat PLA films. Moreover, these films showed reduced light transmission, correlating with decreased weed growth, reduced WVP, and enhanced barrier properties, indicative of improved soil moisture retention. Additionally, PLA films with 4PNC demonstrated greater thermal degradation stability than those with unmodified PNC, suggesting enhanced heat resistance. However, there was no significant difference in aerobic biodegradation between the PLA films with PNC and those with 4PNC. This study confirms that TEVS-modified cellulose significantly enhances the properties of bio-composite films, making them more suitable for mulch film applications.


Asunto(s)
Ananas , Celulosa , Permeabilidad , Hojas de la Planta , Poliésteres , Ananas/química , Celulosa/química , Poliésteres/química , Hojas de la Planta/química , Nanopartículas/química , Vapor
13.
Plant Foods Hum Nutr ; 79(2): 300-307, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38696134

RESUMEN

The industrial processing of pineapples generates a substantial quantity of by-products, including shell, crown, and core. Bromelain, a proteolytic enzyme found naturally in pineapple, including its by-products, may positively influence the bioaccessibility of phenolics from milk coffee. Therefore, this study aimed to assess how the inclusion of extracts from pineapple by-products, namely shell, crown and core, could impact the bioaccessibility of coffee phenolics when combined with milk. After measuring the proteolytic activity of pineapple by-products, the standardized in vitro digestion model of INFOGEST was employed to evaluate changes in total phenolic content, total antioxidant capacity, and individual phenolic compounds in different coffee formulations. The results showed that incorporating extracts from the crown or core in both black and milk coffee increased the bioaccessibility of total phenolics (from 93 to 114% to 105-129%) and antioxidants (from 54 to 56% to 84-87%), while this effect was not observed for the shell. Moreover, adding core extracts also enhanced the bioaccessibility of caffeoylquinic acids and gallic acid in milk coffee (from 0.72 to 0.85% and 109-155%, respectively). Overall, the findings of this study highlight that bromelain from pineapple core may have a favorable effect on the recovery of phenolic compounds in milk coffee, possibly due to its ability to cleave proteins. These outcomes point out that industrial by-products can be transformed into economic value by being reintroduced into the production process through suitable treatment instead of disposal.


Asunto(s)
Ananas , Antioxidantes , Café , Leche , Fenoles , Ananas/química , Fenoles/análisis , Antioxidantes/análisis , Café/química , Leche/química , Bromelaínas , Animales , Ácido Gálico/análisis , Digestión , Disponibilidad Biológica , Extractos Vegetales/química , Ácido Quínico/análogos & derivados , Ácido Quínico/análisis , Manipulación de Alimentos/métodos
14.
Int J Biol Macromol ; 271(Pt 1): 132571, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38782312

RESUMEN

To obtain efficient natural food packaging materials, we utilized acorn starch (AS)-based film strengthened by feruloylated arabinoxylan (FAX) gel and additional retrogradation treatment to extend the shelf life of Agaricus bisporus (A. bisporus). Fourier transform infrared spectroscopy (FT-IR), confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) analyses showed that due to the strong hydrogen bonding between FAX and starch molecules, physical crosslinking occurred between FAX and starch molecules in the composite film, and the microstructure became more compact. Thermogravimetric, tensile strength and swelling degree analyses indicate that the composite film exhibits better thermal stability, mechanical properties, and waterproofing compared to the pure AS film. Consequently, after five days of storage, the moisture content of the A. bisporus packaged with our composite film was 7.53 times and 5.73 times higher than that of the control group and the commercially available PEF group, respectively. Moreover, it delayed the respiration or transpiration of A. bisporus (lower weight loss, relative conductivity, MDA content). This packaging film developed with the objective of eco-friendly and biodegradability has considerable application potential in food and other industries.


Asunto(s)
Agaricus , Embalaje de Alimentos , Almidón , Xilanos , Xilanos/química , Almidón/química , Agaricus/química , Embalaje de Alimentos/métodos , Conservación de Alimentos/métodos , Geles/química , Resistencia a la Tracción , Ananas/química , Espectroscopía Infrarroja por Transformada de Fourier
15.
Int J Biol Macromol ; 271(Pt 2): 132591, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38788873

RESUMEN

This study focused on synthesis of innovative hydrogels with electric field response from modified pineapple peel cellulose and hericium erinaceus chitosan and gelatin based on Schiff base reaction. A series of hydrogels were prepared by oxidized hydroxyethyl cellulose, gelatin and chitosan at different deacetylation degree via mild Schiff base reaction. Subsequently experiments towards the characterization of oxidized hydroxyethyl cellulose/gelatin/chitosan (OHGCS) hydrogel polymers were carried out by FTIR/XRD/XPS, swelling performances and electric response properties. The prepared hydrogels exhibited stable and reversible bending behaviors under repeated on-off switching of electric fields, affected by ionic strength, electric voltage and pH changes. The swelling ratio of OHGCS hydrogels was found reduced as deacetylation degree increasing and reached the maximum ratio âˆ¼ 2250 % for OHGCS-1. In vitro drug releasing study showed the both curcumin loading capacity and release amount of Cur-OHGCS hydrogels arrived about 90 % during 6 h. Antioxidation assessments showed that the curcumin-loaded hydrogels had good antioxidation activities, in which, 10 mg Cur-OHGCS-1 hydrogel could reach to the maximum of about 90 % DPPH scavenging ratio. These results indicate the OHGCS hydrogels have potentials in sensor and drug delivery system.


Asunto(s)
Ananas , Antioxidantes , Celulosa , Quitosano , Curcumina , Gelatina , Hidrogeles , Quitosano/química , Gelatina/química , Curcumina/química , Hidrogeles/química , Ananas/química , Celulosa/química , Celulosa/análogos & derivados , Antioxidantes/química , Antioxidantes/farmacología , Liberación de Fármacos , Basidiomycota/química , Portadores de Fármacos/química , Concentración de Iones de Hidrógeno , Electricidad , Celulosa Oxidada/química , Sistemas de Liberación de Medicamentos
16.
J Proteome Res ; 23(5): 1583-1592, 2024 May 03.
Artículo en Inglés | MEDLINE | ID: mdl-38651221

RESUMEN

MD2 pineapple (Ananas comosus) is the second most important tropical crop that preserves crassulacean acid metabolism (CAM), which has high water-use efficiency and is fast becoming the most consumed fresh fruit worldwide. Despite the significance of environmental efficiency and popularity, until very recently, its genome sequence has not been determined and a high-quality annotated proteome has not been available. Here, we have undertaken a pilot proteogenomic study, analyzing the proteome of MD2 pineapple leaves using liquid chromatography-mass spectrometry (LC-MS/MS), which validates 1781 predicted proteins in the annotated F153 (V3) genome. In addition, a further 603 peptide identifications are found that map exclusively to an independent MD2 transcriptome-derived database but are not found in the standard F153 (V3) annotated proteome. Peptide identifications derived from these MD2 transcripts are also cross-referenced to a more recent and complete MD2 genome annotation, resulting in 402 nonoverlapping peptides, which in turn support 30 high-quality gene candidates novel to both pineapple genomes. Many of the validated F153 (V3) genes are also supported by an independent proteomics data set collected for an ornamental pineapple variety. The contigs and peptides have been mapped to the current F153 genome build and are available as bed files to display a custom gene track on the Ensembl Plants region viewer. These analyses add to the knowledge of experimentally validated pineapple genes and demonstrate the utility of transcript-derived proteomics to discover both novel genes and genetic structure in a plant genome, adding value to its annotation.


Asunto(s)
Ananas , Genoma de Planta , Proteínas de Plantas , Proteogenómica , Espectrometría de Masas en Tándem , Ananas/genética , Ananas/química , Proteogenómica/métodos , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Cromatografía Liquida , Proteoma/genética , Proteoma/análisis , Anotación de Secuencia Molecular , Hojas de la Planta/genética , Hojas de la Planta/química , Péptidos/genética , Péptidos/análisis , Péptidos/química
17.
Food Chem ; 451: 139417, 2024 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-38678651

RESUMEN

In this study, an antibacterial material (CNF@CoMn-NS) with oxidase-like activity was created using ultrathin cobalt­manganese nanosheets (CoMn-NS) with a larger specific surface area grown onto pineapple peel cellulose nanofibrils (CNF). The results showed that the CoMn-NS grew well on the CNF, and the obtained CNF@CoMn-NS exhibited good oxidase-like activity. The imidazole salt framework of the CNF@CoMn-NS contained cobalt and manganese in multiple oxidation states, enabling an active redox cycle and generating active oxygen species (ROS) such as singlet molecular oxygen atoms (1O2) and superoxide radical (·O2-), resulting in the significant inactivation of Staphylococcus aureus (74.14%) and Escherichia coli (54.87%). Importantly, the CNF@CoMn-NS did not exhibit cytotoxicity. The CNF@CoMn-NS further self-assembled into a CNF@CoMn-NS paper with flexibility, stability, and antibacterial properties, which can effectively protect the wound of two varieties of pears from decay caused by microorganisms. This study demonstrated the potential of using renewable and degradable CNF as substrate combined with artificial enzymes as a promising approach to creating antibacterial materials for food preservation and even extending to textiles and biomedical applications.


Asunto(s)
Ananas , Antibacterianos , Celulosa , Escherichia coli , Conservación de Alimentos , Frutas , Nanofibras , Staphylococcus aureus , Ananas/química , Celulosa/química , Celulosa/farmacología , Antibacterianos/farmacología , Antibacterianos/química , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo , Frutas/química , Frutas/microbiología , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Nanofibras/química , Oxidorreductasas/química , Oxidorreductasas/metabolismo , Oxidorreductasas/genética , Pruebas de Sensibilidad Microbiana
18.
Chem Biodivers ; 21(5): e202400315, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38484117

RESUMEN

Pineapple (Ananas comosus), the succulent and vibrant tropical fruit, is a symbol of exoticism and sweetness that captures the hearts and palates of people around the world. The pineapple peel, often considered as waste, has garnered attention for its potential applications. The pineapple peel is rich in essential nutrients, including calcium, potassium, vitamin C, carbohydrates, dietary fiber, and water, making it beneficial for the digestive system, weight management, and overall balanced nutrition. It contains significant amounts of sugars such as sucrose, glucose, and fructose, along with citric acid as the predominant organic acid. The peel also contains bromelain, a proteolytic enzyme known for its digestive properties. Studies have highlighted the pharmacological properties of pineapple peel, such as its potential anti-parasitic effects, alleviation of constipation, and benefits for individuals with irritable bowel syndrome (IBS). Efforts are being made to promote the utilization of pineapple peel as a valuable resource rather than mere waste. Its applications range from the production of vinegar, alcohol, and citric acid to the development of various food products, including squash, syrup, jelly, and pickles. Further research and innovation are required to fully explore the potential of pineapple peel and establish sustainable practices for its utilization, contributing to waste reduction and the development of value-added products.


Asunto(s)
Ananas , Humanos , Ananas/química , Frutas/química , Valor Nutritivo , Extractos Vegetales/química , Extractos Vegetales/farmacología , Ácido Ascórbico/química , Potasio/química , Carbohidratos/química
19.
Environ Sci Pollut Res Int ; 31(16): 24113-24128, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38436853

RESUMEN

The presence of organic dyes in aquatic systems poses a significant threat to ecosystems and human well-being. Due to recycling challenges, traditional commercial activated carbon is not cost-effective. To address this, an imidazolate acid zeolite framework-8 (ZIF-8)-modified magnetic adsorbent (ZMPLB-800) was synthesized through the in-situ formation of ZIF-8 and subsequent carbonization at 800 °C, using magnetic pineapple leaf biochar (MPLB) as a carrier. The porous structure of ZMPLB-800 facilitates the rapid passage of dye molecules, enhancing adsorption performance. ZMPLB-800 exhibited remarkable adsorption capacity for methylene blue (MB) across a pH range of 3-13, with a maximum adsorption capacity of 455.98 mg g-1. Adsorption kinetics and thermodynamics followed the pseudo-second-order kinetic model and Langmuir isotherm model. Mechanisms of MB adsorption included pore filling, hydrogen bonding, electrostatic interactions, π-π interactions, and complexation through surface functional groups. Additionally, ZMPLB-800 demonstrated excellent regeneration performance, recording a removal efficiency exceeding 87% even after five adsorption/desorption cycles. This study provides a novel strategy for treating dye wastewater with MOF composites, laying the foundation for waste biomass utilization.


Asunto(s)
Ananas , Contaminantes Químicos del Agua , Zeolitas , Adsorción , Carbón Orgánico/química , Ecosistema , Concentración de Iones de Hidrógeno , Cinética , Fenómenos Magnéticos , Azul de Metileno/química , Hojas de la Planta , Aguas Residuales , Contaminantes Químicos del Agua/química
20.
Bioprocess Biosyst Eng ; 47(8): 1151-1161, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38536485

RESUMEN

A considerable amount of fruit waste is being produced every day worldwide. The green synthesis of metal nanoparticles from fruit peel waste can be an innovative, cost-effective, and eco-friendly alternative to traditional methods. Copper nanoparticles (CuNPs) were synthesized by a green method using the pineapple peels extract (PLX) and copper sulfate pentahydrate. The formation of CuNPs was visually identified and detected by UV-Visible spectroscopy. The CuNPs were characterized by Fourier-transform infrared (FTIR) spectroscopy, particle size analyzer, scanning electron microscopy (SEM), and X-ray diffraction (XRD). The antioxidant and reducing power of CuNPs were conducted by %DPPH scavenging and electron transfer-based ferric reducing antioxidant power (FRAP) assay, respectively. The antibacterial properties of CuNPs were determined in gram-positive, and gram-negative bacteria. The results showed that the CuNPs were spherical in shape with mean particle size 290.5 nm. The zeta potential of the nanoparticles was found to be - 12.3 mV indicating the instability in the colloidal state. The FTIR study confirmed the peaks of phytochemicals present in the PLX and the nanoparticles supporting the use of pineapple peels as stabilizing, reducing and capping agents. Both the DPPH and reducing power assay depicted that the synthesized CuNPs had significant antioxidant activity. However, the synthesized CuNPs had strong inhibitory capacity against both gram-positive and gram-negative test organisms. Thus, the CuNPS could be used for its viable antibacterial potential to preserve fruits, flowers, and vegetables from bacterial contamination.


Asunto(s)
Ananas , Antibacterianos , Cobre , Tecnología Química Verde , Nanopartículas del Metal , Ananas/química , Antibacterianos/farmacología , Antibacterianos/química , Nanopartículas del Metal/química , Cobre/química , Antioxidantes/química , Antioxidantes/farmacología , Espectroscopía Infrarroja por Transformada de Fourier , Extractos Vegetales/química , Pruebas de Sensibilidad Microbiana , Tamaño de la Partícula , Difracción de Rayos X
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