RESUMEN
As a widely used medicinal plant, Alisma orientale is always a possible target for fraudulent labeling. The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrDNA) of the six species of genus Alisma were sequenced, and two variant sites were found to be specific for A. orientale. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and amplification refractory mutation system (ARMS) analysis were applied to the ITS region for the identification of A. orientale. A restriction site for PSTI useful for PCR-RFLP analysis was detected and a pair of diagnostic primers DFZX-JB02S and DFZX-JB02X were designed for ARMS.
Asunto(s)
Alisma/genética , ADN de Plantas/análisis , ADN Espaciador Ribosómico/análisis , Fitoterapia , Alisma/clasificación , Cartilla de ADN , Humanos , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de RestricciónRESUMEN
OBJECTIVE: To study the seed quality of Alisma orientalis (Sam.) Juzep. METHOD: Collecting seeds from different habitats and studying the thousand-grain weight, moisture content, purity and germination percentage. RESULT: The seeds reaped in summer friom Pengshan, Sichuan province are better than those from other habitats. Its thousand-grain weight is 0.4682 g, moisture content is 12.48%, purity of seeds is 89.18% and germination percentage is above 95%. CONCLUSION: The cause of qualitative differences in seeds of Alisma orientalis from different habitats were analysed in this paper.
Asunto(s)
Alisma/química , Alisma/crecimiento & desarrollo , Plantas Medicinales/química , Semillas/química , Alisma/clasificación , Peso Corporal , Plantas Medicinales/clasificación , Plantas Medicinales/crecimiento & desarrollo , Control de Calidad , Estaciones del Año , Semillas/clasificación , Semillas/crecimiento & desarrollo , Temperatura , Agua/análisisRESUMEN
The paper reported that UV and IR spectrum were used to identify Alisma orientalis and A. canaliculatum. The results of determination showed that the solution of methanol extraction of A. orientalis absorbed in 204.1 +/- 0.5 nm, but which of Alisma canaliculatum absorbed in 205.7 nm and 285 nm in UV spectrum. The dry substance of water extraction of A. orientalis absorbed in 3402, 2933, 1642, 1054 and 926 cm-1 in IR spectrum. IR spectrum of A. orientalis is different from which of Alisma canaliculatum in fingerprint.