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The plasma bacterial killing ability (BKA) is modulated by the stress response in vertebrates, including amphibians. The complement system is an effector mechanism comprised of a set of proteins present in the plasma that once activated can promote bacterial lysis. Herein, we investigated whether changes in plasma BKA as a result of the acute stress response and an immune challenge are mediated by the complement system in Rhinella diptycha toads. Additionally, we investigated whether the observed changes in plasma BKA are associated with changes in plasma corticosterone levels (CORT). We subjected adult male toads to a restraint or an immune challenge (with three concentrations of Aeromonas hydrophila heat inactivated), and then evaluated the plasma BKA against A. hydrophila, in vitro. We determined the complement system activity on plasma BKA, by treating the plasma (baseline, 1 h and 24 h post-restraint, and after the immune challenge) with ethylenediaminetetraacetic acid, heat, or protease. Our results showed increased CORT 1 h and 24 h after restraint and decreased plasma BKA 24 h post-restraint. The inhibitors of the complement system decreased the plasma BKA compared with untreated plasma at all times (baseline, 1 h, and 24 h after restraint), demonstrating that the plasma BKA activity is partially mediated by the complement system. The immune challenge increased CORT, with the highest values being observed in the highest bacterial concentration, compared with control. The plasma BKA was not affected by the immune challenge but was demonstrated to be partially mediated by the complement system. Our results demonstrated that restraint and the immune challenge activated the hypothalamus-pituitary-interrenal axis, by increasing plasma CORT levels in R. diptycha. Also, our results demonstrated the complement system is participative in the plasma BKA for baseline and post-stress situations in these toads.

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This study investigated the effects of dietary supplementation with Mansoa alliacea hydroalcoholic extracts on growth, blood and immune parameters of Arapaima gigas. Fish were fed for 30 days with diets enriched with 0, 4, 8, and 12 g kg-1 of M. alliacea hydroalcoholic extract and subjected to infection with Aeromonas hydrophila and handling stress. Fish fed with 8 g kg-1 of extract showed significant increase in final weight, specific growth rate and feed efficiency when compared to the other groups. Glucose, triglycerides, total proteins, and globulins increased significantly in fish fed with 8 g kg-1 of extract, whereas albumin decreased. The number of thrombocytes increased significantly with the dietary supplementation of 8 and 12 g kg-1 of extract. After the challenge with A. hydrophila and handling stress, fish fed with 8 g kg-1 of extract had significantly higher levels of glucose, globulins, and albumins, and fish fed with 8 and 12 g kg-1 of extract showed an increment of respiratory burst. Triglyceride levels dropped significantly in fish fed with 4, 8, and 12 g kg-1 of extract, whereas the number of neutrophils increased, and total thrombocytes, leukocytes and lymphocytes were higher in fish fed with 12 g kg-1 of extract. Dietary supplementation with M. alliacea extract at 8 g kg-1 was efficient in improving the growth and innate immunity of A. gigas, being potentially useful in fish farming to control the development of A. hydrophila infections.(AU)

Investigou-se os efeitos da suplementação com extrato hidroalcólico de Mansoa alliacea sobre o crescimento e parâmetros sanguíneos e imunológicos de Arapaima gigas. Os peixes foram alimentados por 30 dias com dietas enriquecidas com 0, 4, 8 e 12 g kg-1 de extrato hidroalcoólico de M. alliacea e submetidos à infecção por Aeromonas hydrophila e estresse de manejo. Os peixes alimentados com 8 g kg-1 de extrato apresentaram aumento significativo no peso final, taxa de crescimento específico e eficiência alimentar quando comparados aos demais grupos. Glicose, triglicerídeos, proteínas totais e globulinas aumentaram significativamente nos peixes alimentados com 8 g kg-1 de extrato, enquanto a albumina diminuiu. O número de trombócitos aumentou significativamente com a suplementação dietética de 8 e 12 g kg-1 de extrato. Após infecção com A. hydrophila e estresse de manejo, os peixes alimentados com 8 g kg-1 de extrato apresentaram níveis significativamente mais altos de glicose, globulinas e albuminas, e os peixes alimentados com 8 e 12 g kg-1 de extrato apresentaram incremento de explosão respiratória. Os níveis de triglicerídeos decresceram nos peixes alimentados com 4, 8 e 12 g kg-1 de extrato, enquanto o número de neutrófilos aumentou, e o número total de trombócitos, leucócitos e linfócitos foi maior nos peixes alimentados com 12 g kg-1 de extrato. A suplementação com a 8 g kg-1 de extrato de M. alliacea foi eficiente em melhorar o crescimento e a imunidade inata de A. gigas, sendo potencialmente útil na piscicultura para controlar o desenvolvimento de infecções por A. hydrophila.(AU)

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Aeromonas hydrophila is responsible for outbreaks of a severe infectious disease in fish farms around the world and is one of the major causes of economic losses to the neotropical fish farmers. This study assessed the induction of immune responses and protection against A. hydrophila in pacu, Piaractus mesopotamicus, vaccinated through intraperitoneal and immersion route with inactivated virulent strain. Fish were randomly distributed in three vaccinated groups: intraperitoneal (i.p.) route; immersion; and immersion + booster; and control group (unvaccinated). All vaccination protocols used the concentration of 1.7 × 108 CFU mL-1 of inactivated A. hydrophila., and an oil adjuvant was used for vaccine prepararion for i.p. route vaccination. Blood and skin mucus from 9 fishes per treatment were collected at 14, 28, 42 and 84 days post-vaccination (DPV) for determination of lysozyme concentration in skin mucus, as well as antibodies anti-A. hydrophila in blood serum and skin mucus. Fish were challenged at 84 DPV with homologous and virulent strain of A. hydrophila for evaluation of resistance against bacterial infection. The results demonstrated that vaccination with inactivated A. hydrophila suspension by i.p. or immersion resulted in significant increase of skin mucus lysozyme and specific antibody levels in serum and skin mucus, at 28 and 42 DPV, and this increase in innate and adaptive immunity remained significant in pacu vaccinated through i.p. route up to 84 DPV. Although no significant differences were observed in the survival study, pacu vaccinated through i.p. route presented 31,33% of relative percentage survival (RPS) in LD50-96h when compared unvaccinated fish challenged at 84 DPV. The results observed in this study indicate that vaccination programs with inactivated A. hydrophila, including booster doses by i.p. or immersion routes, could result in more effective protection in pacu against this bacteriosis, by increasing innate and adaptive mucosal and systemic immune responses.

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This study attempts to describe the immunostimulatory effects of three fungal glucans on innate immunity responses in an in vitro assays using Pacific red snapper leukocytes. First, the yield glucans obtained was higher in Aspergillus niger, follow by Aspergillus ochraceus and Alternaria botrytis (40, 20 and 10%, respectively). Structural characterization of these fungal glucans by proton nuclear magnetic resonance (NMR) indicated structures containing (1-6)-branched (1-3)-ß-D-glucan. The immunostimulatory activity of fungal glucans were assessed in head-kidney leukocytes at 24 h using colorimetric assays and molecular gene expression. In addition, the response against bacterial infection using Aeromonas hydrophila was evaluated by flow cytometry with annexin V/propidium iodide. Leukocytes responded positively to fungal glucans where the viability was higher than 80%. Interestingly, A. niger ß-glucans enhanced the phagocytic ability and capacity in head-kidney leukocytes. Immunological assays reveled an increased in nitric oxide production, myeloperoxidase, superoxide dismutase and catalase activities, in fish stimulated with A. niger ß-glucans. Induction of cytokines (IL-1ß, TNF-α, IL-6, IL-8 and IL-12) were more pronounced in A. niger ß-glucans leukocytes stimulated compared to other group. Finally, flow cytometry assay showed that A. botrytis and A. niger ß-glucans were able to inhibit apoptosis caused by Aeromonas hydrophila in the Pacific red snapper leukocytes indicating an immunostimulant potent response by fungi derived-glucans. These results strongly support the idea that fungal ß-glucans can stimulate the immune mechanism in head-kidney leukocytes and that Aspergillus niger ß-glucan possess immunostimulatory properties cell increasing viability, and reducing necrotic cell death caused by Aeromonas hydrophila.

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In response to pathogens, the higher vertebrate innate immune system activates pro-inflammatory caspase-1 which is responsible for the processing and secretion of several important cytokines involved in the host's defence against infection. To date, caspase-1 has been described in few teleost fish, and its activity has been demonstrated through substrate cleavage and inhibition by pharmacological agents. In this study, the detection of the active form of caspase-1 during the immune response in salmonid fish is described, where two antibodies were produced. These antibodies differentially recognize the structural epitopes of the inactive pro-caspase-1 and the processed active form of the caspase. Firstly, caspase-1 activation was demonstrated in vitro by ELISA, Western blotting and immunocytochemistry in rainbow trout macrophages exposed to different pathogen-associated molecular patterns plus the pathogen Aeromonas hydrophila. This activity was clearly abrogated by a caspase inhibitor and seems to be unrelated to IL-1ß secretion. Caspase-1 activation was then validated in vivo in gill cells from fish challenged with Aeromonas salmonicida. These results represent the first demonstration of caspase-1 activation in salmonids, and the first evidence of the putative regulatory role which this protease plays in inflammatory response in this fish group, as described for some other teleosts and mammals.

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TWEAK and APRIL are important members of the TNF superfamily, which play a crucial role in several diseases. Here, we describe the identification of grass carp (Ctenopharyngodon idella) homologs of TWEAK and APRIL (designated gcTWEAK and gcAPRIL, respectively) and their response to Aeromonas hydrophila and Aquareovirus infection. The gcTWEAK cDNA sequence contains 2273 bases with an open reading frame of 753 bases encoding 250-amino acid residues. The gcTWEAK protein contains a predicted transmembrane domain, a putative furin protease cleavage site, 3 conserved cysteine residues, and a typical TNF homology domain. The gcAPRIL cDNA sequence contains 1408 bases with an open reading frame of 747 bases encoding 248-amino acid residues. The gcAPRIL protein contains a predicted transmembrane domain, a putative furin protease cleavage site, 2 conserved cysteine residues, and a typical TNF homology domain corresponding to other, known APRIL homologs. Reverse transcription-polymerase chain reaction analysis shows that both gcTWEAK and gcAPRIL transcripts are predominantly expressed in the skin, spleen, and head kidney, and they are significantly upregulated in most immune tissues by A. hydrophila and Aquareovirus infections. Our results demonstrate that liver is the most responsive tissue against bacterial infection, whereas gill is the most responsive tissue against viral infection. The association of increased gcTWEAK and gcAPRIL expression after bacterial and viral infections suggests that they play a potentially important role in the immune system of fish.

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Antibody can be assessed by agglutinating antibody titer which is a quantitative measure of circulating antibodies in serum from fish previously immunized. The antibody evaluation has been performed with different fish species, and is considered a reliable method that can be applied to confirm several hypothesis regarding acquired immunity, even in conjunction with precise methods to describe immune mechanisms. In order to provide appropriate analytical methods for future studies on the specific immune system of native fish, the present study standardized on assay to measure the serum agglutinating antibody titer produced after immunization with inactivated A. hydrophila and levamisole administration in pacu. It was possible to determine the agglutinating antibodies titer in a satisfactorily way in pacu immunized with inactive A. hydrophila, and the highest titers were observed on fish fed with levamisole.

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Fish vaccination has been increasingly exploited as a tool to control pathogen infection. The production of immunoglobulin following vaccination might be affected by several factors such as management procedures, water temperature, and the presence of xenobiotics. In the present study, we aimed to investigate the kinetics of immunoglobulin production in silver catfish (Rhamdia quelen) inoculated with inactivated Aeromonas hydrophila and kept at two different water temperatures (17.4±0.46 or 21.3±0.36C). The effect of a second antigen inoculation and exposure of fish to sublethal concentrations of the herbicides atrazine and glyphosate at 10% of the lethal concentration (LC50-96h) on specific serum antibodies were also investigated. Antibodies to A. hydrophila were detected as early as 7 days post-inoculation and increased steadily up to 35 days. The kinetics of antibody production were similar in fish kept at 17.4±0.46 and 21.3±0.36C, and reinoculation of antigen at 21 days after priming failed to increase specific antibody levels. Intriguingly, we found that, in fish exposed to atrazine and glyphosate, the secretion of specific antibodies was higher than in non-exposed inoculated fish. These findings are important for the design of vaccines and vaccination strategies in Neotropical fish species. However, because atrazine and glyphosate are widespread contaminants of soil and water, their immune-stimulating effect could be harmful, in that fish living in herbicide-contaminated water might have increased concentrations of nonspecific antibodies that could mediate tissue injury.

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Antibody can be assessed by agglutinating antibody titer which is a quantitative measure of circulating antibodies in serum from fish previously immunized. The antibody evaluation has been performed with different fish species, and is considered a reliable method that can be applied to confirm several hypothesis regarding acquired immunity, even in conjunction with precise methods to describe immune mechanisms. In order to provide appropriate analytical methods for future studies on the specific immune system of native fish, the present study standardized on assay to measure the serum agglutinating antibody titer produced after immunization with inactivated A. hydrophila and levamisole administration in pacu. It was possible to determine the agglutinating antibodies titer in a satisfactorily way in pacu immunized with inactive A. hydrophila, and the highest titers were observed on fish fed with levamisole.

Os anticorpos podem ser avaliados pelo título aglutinante de anticorpos, que é uma medida quantitativa de anticorpos no soro de peixe previamente imunizados. A determinação do título de anticorpos foi realizada com diversas espécies de peixes e é considerado um método confiável que pode ser aplicado para confirmar diversas hipóteses que envolvam o sistema adquirido de defesa, mesmo em conjunto com métodos precisos, para descrever mecanismos imunes. A fim de prover métodos analíticos adequados para futuros estudos sobre o sistema imune específico de peixes nativos, o presente estudo aperfeiçoou o ensaio para avaliar o título aglutinante de anticorpos em soro de pacu imunizados com A. hydrophila e alimentados com levamisol. Foi possível determinar o título aglutinante de anticorpos de forma satisfatória, em pacus imunizados com A. hydrophila inativa, e os maiores títulos foram observados em peixes alimentados com levamisol.

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Antibody can be assessed by agglutinating antibody titer which is a quantitative measure of circulating antibodies in serum from fish previously immunized. The antibody evaluation has been performed with different fish species, and is considered a reliable method that can be applied to confirm several hypothesis regarding acquired immunity, even in conjunction with precise methods to describe immune mechanisms. In order to provide appropriate analytical methods for future studies on the specific immune system of native fish, the present study standardized on assay to measure the serum agglutinating antibody titer produced after immunization with inactivated A. hydrophila and levamisole administration in pacu. It was possible to determine the agglutinating antibodies titer in a satisfactorily way in pacu immunized with inactive A. hydrophila, and the highest titers were observed on fish fed with levamisole.(AU)

Os anticorpos podem ser avaliados pelo título aglutinante de anticorpos, que é uma medida quantitativa de anticorpos no soro de peixe previamente imunizados. A determinação do título de anticorpos foi realizada com diversas espécies de peixes e é considerado um método confiável que pode ser aplicado para confirmar diversas hipóteses que envolvam o sistema adquirido de defesa, mesmo em conjunto com métodos precisos, para descrever mecanismos imunes. A fim de prover métodos analíticos adequados para futuros estudos sobre o sistema imune específico de peixes nativos, o presente estudo aperfeiçoou o ensaio para avaliar o título aglutinante de anticorpos em soro de pacu imunizados com A. hydrophila e alimentados com levamisol. Foi possível determinar o título aglutinante de anticorpos de forma satisfatória, em pacus imunizados com A. hydrophila inativa, e os maiores títulos foram observados em peixes alimentados com levamisol.(AU)

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A total of 360 pacus (Piaractus mesopotamicus) were used to study vascular permeability (VP) and inflammatory cell component (CC) in induced aerocystitis in P. mesopotamicus through inoculation of inactivated Aeromonas hydrophila, and the effect of steroidal and nonsteroidal anti-inflammatory drugs. It was observed that after inoculation of A. hydrophila, the maximum VP occurred 180 min post-stimulus (MPS). Pretreatment with anti-inflammatory drugs inhibited VP, and the inhibitory effect of dexamethasone was seen earlier than the effects caused by meloxicam and indomethacin. Inoculation of the bacterium caused a gradual increase in the accumulation of cells, which reached a maximum 24 h post-stimulus (HPS). Pretreatment with dexamethasone, indomethacin and meloxicam reduced the accumulation of lymphocytes, thrombocytes, granulocytes and macrophages. There was no significant difference between the different doses of the drugs tested. The results suggest that eicosanoids and pro-inflammatory cytokines participate in chemical mediation in acute inflammation in pacus.

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The Gram-negative bacteria Aeromonas hydrophila is a heterogeneous organism that causes the disease known as motile aeromonad septicaemia, which is responsible for serious economic loss in seabream culture due to bacterial infections. However, the immune mechanisms involved in this disease in fish are still poorly understood. For the purpose of this study, gilthead seabream (Sparus aurata L.) specimens received a double intraperitoneal injection of bacterial inoculums: a primary infection with 1 × 10(7) cell ml(-1) A. hydrophila, followed by a secondary infection with 1 × 10(8) cell ml(-1) fourteen days later. Changes in cellular innate immune parameters - phagocytosis, respiratory burst activity and peroxidase leucocyte content - were evaluated 24 and 48 h after each injection. Simultaneously, the expression levels of nine immune-relevant genes (TLR, NCCRP-1, HEP, TCR, IgM, MHC-IIα, IL-1ß, C3 and CSF-1R) were measured in the head-kidney, spleen, intestine and liver, by using q-PCR. Generally, the results showed a significant decrease in cellular immune responses during the primary infection and a significant enhanced during the second infection, principally in respiratory burst and peroxidase activity, thus indicating a recovery of the immune system against this bacterial pathogen. Finally, transcript levels of immune genes were down-regulated during the first infection, except for the IL-1ß gene. In contrast, mRNA expression levels during the re-infection were significantly up-regulated. The results seem to suggest a relatively fast elimination of the bacteria and recovery of fish during the secondary infection.

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Determinou-se a concentração eficaz de oxitetraciclina (OTC) e florfenicol (FFC) no tratamento de Aeromonas hydrophila em pacu (Piaractus. mesopotamicus). Os pacus foram submetidos à captura duas vezes ao dia por quatro dias e em seguida foram infectados com A. hydrophila (2,4x10(7) bactéria mL-1). Os tratamentos utilizados foram: controle sem infecção (CSI), controle com infecção (CCI) e tratados com 110,0; 140,0 e 170,0mgOTC.kg-1, e 5,0; 10,0 e 15,0mgFFC.kg-1. As variáveis de qualidade da água foram monitoradas diariamente. Após o tratamento, no CSI dos dois testes, ocorreu 100 por cento de sobrevivência. Nos testes com OTC, no CCI, a sobrevivência foi de 29,2 por cento; em 110,0mg.kg-1, 37,5 por cento; em 140,0mg.kg-1, 29,2 por cento; e em 170,0mg.kg-1, 50,0 por cento. Nos testes com FFC, foi eficaz com 10,0mg.kg-1, e no CCI a sobrevivência foi de 76,9 por cento; em 5,0mg.kg-1, 81,81 por cento; em 10,0mg/L.kg-1, 100 por cento e em 15,0mg.kg-1, 87,5 por cento. A OTC, em concentrações de até 170,0mg.kg-1 de ração, não é eficaz para o controle de A. hydrophila em pacu, e o FFC é eficaz na concentração de 10,0mg.kg-1 e ambos não alteram as variáveis de qualidade de água.

The effective concentration of antibiotics OTC and FFC in the treatment of Aeromonas hydrophila in pacu (Piaractus mesopotamicus). The pacus were subjected to capture twice daily (four days) and then were infected with A. hydrophila (2.4 x10 7 bacteria mL -1 ). The treatments were: control without infection (CSI), with infection control (CCI) and 110.0, 140.0 and 170.0mgOTC.kg -1 , and 5.0, 10.0 and 15.0mgFFC.kg -1 . The variables of water quality were monitored daily. After treatment, the CSI of the two experiments was 100 percent survival. In tests with OTC, the CCI was 29.17 percent, in 110.0mg.kg -1 , 37.5 percent, in 140.0mg.kg -1 , 29.17 percent and in 170.0mg.kg -1 , 50.0 percent. The FFC was effective with 10.0 mg kg -1 , and in the CCI the survival was 76.9 percent, in 5.0mg.kg -1 , 81.81 percent, in 10.0mg/L.kg -1 , 100 percent and in 15.0mg.kg -1 , 87.5 percent. The OTC in concentrations of up to 170.0 mg.kg -1 of ration is not effective in the control of A. hydrophila in pacu and the FFC is effective in the concentration of 10.0mg.kg -1 and this antibiotic does not change the variables of water quality.

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Determinou-se a concentração eficaz de oxitetraciclina (OTC) e florfenicol (FFC) no tratamento de Aeromonas hydrophila em pacu (Piaractus. mesopotamicus). Os pacus foram submetidos à captura duas vezes ao dia por quatro dias e em seguida foram infectados com A. hydrophila (2,4x10(7) bactéria mL-1). Os tratamentos utilizados foram: controle sem infecção (CSI), controle com infecção (CCI) e tratados com 110,0; 140,0 e 170,0mgOTC.kg-1, e 5,0; 10,0 e 15,0mgFFC.kg-1. As variáveis de qualidade da água foram monitoradas diariamente. Após o tratamento, no CSI dos dois testes, ocorreu 100 por cento de sobrevivência. Nos testes com OTC, no CCI, a sobrevivência foi de 29,2 por cento; em 110,0mg.kg-1, 37,5 por cento; em 140,0mg.kg-1, 29,2 por cento; e em 170,0mg.kg-1, 50,0 por cento. Nos testes com FFC, foi eficaz com 10,0mg.kg-1, e no CCI a sobrevivência foi de 76,9 por cento; em 5,0mg.kg-1, 81,81 por cento; em 10,0mg/L.kg-1, 100 por cento e em 15,0mg.kg-1, 87,5 por cento. A OTC, em concentrações de até 170,0mg.kg-1 de ração, não é eficaz para o controle de A. hydrophila em pacu, e o FFC é eficaz na concentração de 10,0mg.kg-1 e ambos não alteram as variáveis de qualidade de água.(AU)

The effective concentration of antibiotics OTC and FFC in the treatment of Aeromonas hydrophila in pacu (Piaractus mesopotamicus). The pacus were subjected to capture twice daily (four days) and then were infected with A. hydrophila (2.4 x10 7 bacteria mL -1 ). The treatments were: control without infection (CSI), with infection control (CCI) and 110.0, 140.0 and 170.0mgOTC.kg -1 , and 5.0, 10.0 and 15.0mgFFC.kg -1 . The variables of water quality were monitored daily. After treatment, the CSI of the two experiments was 100 percent survival. In tests with OTC, the CCI was 29.17 percent, in 110.0mg.kg -1 , 37.5 percent, in 140.0mg.kg -1 , 29.17 percent and in 170.0mg.kg -1 , 50.0 percent. The FFC was effective with 10.0 mg kg -1 , and in the CCI the survival was 76.9 percent, in 5.0mg.kg -1 , 81.81 percent, in 10.0mg/L.kg -1 , 100 percent and in 15.0mg.kg -1 , 87.5 percent. The OTC in concentrations of up to 170.0 mg.kg -1 of ration is not effective in the control of A. hydrophila in pacu and the FFC is effective in the concentration of 10.0mg.kg -1 and this antibiotic does not change the variables of water quality.(AU)

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Endosulfan is a persistent organochlorine insecticide which is extremely toxic to fish. It is known to induce immunological alterations in juvenile Nile tilapia (Oreochromis niloticus) such as increases in phagocytic activity and reactive oxygen species production of spleen macrophages. The purpose of the present study was to demonstrate the effects of acute exposure to a sublethal concentration of endosulfan (7 ppb, 96 h) on parameters of the adaptive humoral immune response of the aforementioned aquatic organism. The effect of endosulfan on the capacity of immune cells to produce interleukin-2 like (IL-2L) factor and immunoglobulin M (IgM) in response to a challenge with (1/2) LD50 of the infectious bacteria Aeromonas hydrophila was evaluated. Experimental results indicate that short, sublethal, endosulfan exposure triggers a succession of events beginning with non-specific activation of macrophages followed by an exacerbated synthesis of the IL-2L factor by activated B cells. This leads to significantly increased secretion of IgM and could in turn facilitate autoantibody production and the development of autoimmune pathologies.

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AIMS: to develop a monoclonal antibody (MAb) for the rapid detection of Aeromonas hydrophila in human faeces. METHODS AND RESULTS: A monoclonal antibody with strong specificity against Aer. hydrophila was obtained by the fusion of myeloma cells and splenocytes of a mouse immunized with vegetative cells of Aer. hydrophila ATCC 7966, followed by a two-step selection against other species of the genera. ELISA analyses revealed that MAb 5F3 strongly reacts with all the Aer. hydrophila strains evaluated, showing a just basal reactivity against other species of the genera, especially Aer. sobria and Aer. caviae. CONCLUSIONS: MAb 5F3 was characterized as an IgG that recognized a polypeptide of approximately 110 kDa. SIGNIFICANCE AND IMPACT OF THE STUDY: This MAb could be used to detect Aer. hydrophila in human stool samples.

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