RESUMEN

We describe the detection of ectopic ("illegitimate") transcripts of the proacrosin and protamine 2 genes, which are specific for human spermatogenesis, in non-cultured peripheral blood lymphocytes. After specifically-primed reverse transcription of total lymphocyte RNA, these rare transcripts can be directly visualised after two rounds of polymerase chain reaction with nested primers. Sequence and restriction analyses of the corresponding fragments have confirmed that transcripts of proacrosin and protamine 2 are present in the lymphocytes not only of males, but also of adult females.

Asunto(s)

Acrosina/sangre , Precursores Enzimáticos/sangre , Linfocitos/metabolismo , Protaminas/sangre , ARN Mensajero/sangre , Espermátides/metabolismo , Acrosina/genética , Secuencia de Bases , Precursores Enzimáticos/genética , Femenino , Humanos , Masculino , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Protaminas/genética , Transcripción Genética