RESUMEN

As a highly toxic aldehyde, acrolein is widely found in diet and environment, and can be produced endogenously, posing a serious threat to human health. Herein, we designed a novel fluorescent nanoplatform integrating carbon dots­manganese dioxide (CDs-MnO2) and glutathione (GSH) for all-in-one sensing and removal of acrolein. By converting Mn4+ to free Mn2+, GSH inhibited the inner filter effect (IFE) of MnO2 nanosheets, and the Michael addition of acrolein with GSH inhibited the GSH-induced Mn4+ conversion, forming an "off-on-off" fluorescence response of CDs. The developed fluorescent nanoplatform exhibited high sensitivity (LOD was 0.067 µM) and selectivity for the simultaneous detection and removal of acrolein. The combination of CDs-MnO2 hydrogels with smartphones realized the point-of-care detection of acrolein, yielding satisfactory results (recovery rates varied between 97.01-104.65%, and RSD ranged from 1.42 to 4.16%). Moreover, the capability of the nanoplatform was investigated for on-site evaluating acrolein scavengers' efficacy, demonstrating excellent potential for practical application.

Asunto(s)

Acroleína , Colorantes Fluorescentes , Compuestos de Manganeso , Óxidos , Puntos Cuánticos , Acroleína/química , Compuestos de Manganeso/química , Óxidos/química , Colorantes Fluorescentes/química , Puntos Cuánticos/química , Glutatión/química , Espectrometría de Fluorescencia , Límite de Detección , Carbono/química

RESUMEN

Reactive carbonyl species (RCS), including acrolein (ACR), methylglyoxal (MGO), and glyoxal (GO), are typically generated in food processing and accumulate in the body for ages, triggering various chronic diseases. Here, we investigated the capture capability and reaction pathways of mangiferin one-to-one and one-to-many on RCS in high temperatures using UPLC-MS/MS. We found that mangiferin can capture ACR/MGO/GO to form their adducts, yet, the ability to capture RCS is arranged in different orders, with ACR > MGO > GO for a single RCS and MGO > ACR > GO for multiple RCS. After synthesizing and identifying the structures of the ACR- and MGO-adducts of MGF, our results indicated that MGF-ACR-MGO produced in the multiple-RCS-MGF system was formed by capturing MGO through MGF-ACR rather than through MGF-MGO capturing ACR, which resulting in higher inhibitory activity of MGF against MGO than against ACR. Then, the capture ability and path of MGF on RCS were verified in the coffee-leaves tea and cake.

Asunto(s)

Acroleína , Glioxal , Calor , Piruvaldehído , Espectrometría de Masas en Tándem , Xantonas , Xantonas/química , Piruvaldehído/química , Glioxal/química , Acroleína/química , Acroleína/análogos & derivados , Cromatografía Líquida de Alta Presión , Manipulación de Alimentos

RESUMEN

Our study focuses on synthesizing and exploring the potential of three N-(4) substituted thiosemicarbazones derived from cinnamic aldehyde, alongside their Ru(II)-(η6 -p-cymene)/(η6-benzene) complexes. The synthesized compounds were comprehensively characterized using a range of analytical techniques, including FT-IR, UV-visible spectroscopy, NMR (1H, 13C), and HRMS. We investigated their electronic and physicochemical properties via density functional theory (DFT). X-ray crystal structures validated structural differences identified by DFT. Molecular docking predicted promising bioactivities, supported by experimental observations. Notably, docking with EGFR suggested an inhibitory potential against this cancer-related protein. Spectroscopic titrations revealed significant DNA/BSA binding affinities, particularly with DNA intercalation and BSA hydrophobic interactions. RuPCAM displayed the strongest binding affinity with DNA (Kb = 6.23 × 107 M-1) and BSA (Kb = 9.75 × 105 M-1). Assessed the cytotoxicity of the complexes on cervical cancer cells (HeLa), and breast cancer cells (MCF-7 and MDA-MB 231), revealing remarkable potency. Additionally, selectivity was assessed by examining MCF-10a normal cell lines. The active complexes were found to trigger apoptosis, a vital cellular process crucial for evaluating their potential as anticancer agents utilizing staining assays and flow cytometry analysis. Intriguingly, complexation with Ru(II)-arene precursors significantly amplified the bioactivity of thiosemicarbazones, unveiling promising avenues toward the creation of powerful anticancer agents.

Asunto(s)

Acroleína , Antineoplásicos , Ensayos de Selección de Medicamentos Antitumorales , Simulación del Acoplamiento Molecular , Rutenio , Tiosemicarbazonas , Humanos , Tiosemicarbazonas/química , Tiosemicarbazonas/farmacología , Antineoplásicos/farmacología , Antineoplásicos/química , Antineoplásicos/síntesis química , Rutenio/química , Rutenio/farmacología , Ligandos , Acroleína/análogos & derivados , Acroleína/química , Acroleína/farmacología , Estructura Molecular , Complejos de Coordinación/química , Complejos de Coordinación/farmacología , Complejos de Coordinación/síntesis química , Proliferación Celular/efectos de los fármacos , ADN/metabolismo , ADN/química , Ensayo de Materiales , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Materiales Biocompatibles/síntesis química , Tamaño de la Partícula , Albúmina Sérica Bovina/química , Albúmina Sérica Bovina/metabolismo , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga

RESUMEN

Ginger, valued for its culinary and medicinal properties, suffers substantial production loss-up to 90 %-due to fungal soft rot. To combat this, we have developed an environmentally sustainable antifungal polysaccharide gel derived from a water-soluble Schiff base of O-carboxymethyl chitosan (CMC) and cinnamaldehyde (CIN). Terpene incorporation was confirmed via various characterization techniques, including Fourier transform infrared (FT-IR), pH-dependent release, solubility, thermogravimetric analysis, and UV-vis spectra. Results showed successful grafting of CIN onto the polysaccharide, at a CIN:CMC ratio of 120 mg/g. In vitro evaluation demonstrated significant antifungal activity against F. oxysporum, with a MIC value of 159.25 µg/mL. Application of the CMC=CIN gel to ginger rhizomes inhibited spore germination in all evaluated wounds, enhancing gloss and appearance. These findings validate the efficacy of this novel, environmentally friendly gel in preventing ginger loss caused by fungal infections.

Asunto(s)

Acroleína , Quitosano , Geles , Bases de Schiff , Zingiber officinale , Quitosano/farmacología , Quitosano/química , Quitosano/análogos & derivados , Acroleína/análogos & derivados , Acroleína/farmacología , Acroleína/química , Zingiber officinale/química , Bases de Schiff/farmacología , Bases de Schiff/química , Geles/química , Geles/farmacología , Fusarium/efectos de los fármacos , Fusarium/crecimiento & desarrollo , Enfermedades de las Plantas/microbiología , Antifúngicos/farmacología , Antifúngicos/química , Solubilidad , Conservantes de Alimentos/farmacología , Conservantes de Alimentos/química

RESUMEN

Although cinnamaldehyde (CA) is an excellent antimicrobial agent, its application in the food industry was limited by its volatility and lack of antimicrobial persistence. Herein, aminated hollow mesoporous silica (NH2-HMSN) was prepared by selective etching and amino-modified. Subsequently, long-acting antibacterials with regulated release (NH2-HMSN@CA) were obtained by using NH2-HMSN as cinnamaldehyde carrier. NH2-HMSN@CA can effectively regulate the release of CA, and has 100 % inhibition effect on the growth of E. coli, S. aureus and C. acutatum. In addition, nanocellulose/NH2-HMSN@CA (CHA) coating film was prepared for postharvest preservation of loquat. The coating film effectively improved the storage quality and shelf life of loquat, and delayed the postharvest decay of loquat. The prepared coating film active packaging for long-term preservation is expected to provide a scheme for promoting sustainable preservation of postharvest loquat.

Asunto(s)

Acroleína , Antibacterianos , Celulosa , Conservación de Alimentos , Dióxido de Silicio , Acroleína/análogos & derivados , Acroleína/farmacología , Acroleína/química , Dióxido de Silicio/química , Celulosa/química , Celulosa/análogos & derivados , Conservación de Alimentos/métodos , Antibacterianos/farmacología , Antibacterianos/química , Embalaje de Alimentos/métodos

RESUMEN

A new antibacterial film was constructed to combat the severe spoilage of fruits and vegetables caused by microorganisms. Specifically, photoresponsive cinnamaldehyde-tannic­iron acetate nanospheres (CTF NPs) were prepared using ultrasonic-triggered irreversible equilibrium self-assembly and ionic cross-linking co-driven processes and were integrated into the matrix of κ-carrageenan (KC) (CTF-KC films) as functional fillers. The CTF0.4-KC film (KC film doped with 0.4 mg/mL CTF NPs) showed a 99.99% bactericidal rate against both E. coli and S. aureus, extended the storage period of cherry tomatoes from 20 to 32 days. The introduction of CTF enhanced the barrier, thermal stability, and mechanical strength properties, albeit with a slight compromise on transparency. Furthermore, the biosafety of the CTF0.4-KC film was confirmed through hemolysis and cytotoxicity tests. Together, the aforementioned results demonstrated the outstanding antibacterial and fresh-keeping properties of CTF0.4-KC. These desirable properties highlight the potential use of CTF0.4-KC films in food preservation applications.

Asunto(s)

Antibacterianos , Escherichia coli , Conservación de Alimentos , Staphylococcus aureus , Escherichia coli/efectos de los fármacos , Conservación de Alimentos/instrumentación , Conservación de Alimentos/métodos , Antibacterianos/farmacología , Antibacterianos/química , Staphylococcus aureus/efectos de los fármacos , Embalaje de Alimentos/instrumentación , Carragenina/química , Carragenina/farmacología , Solanum lycopersicum/química , Solanum lycopersicum/microbiología , Humanos , Acroleína/análogos & derivados , Acroleína/química , Acroleína/farmacología , Frutas/química

RESUMEN

Chronic wounds, such as diabetic ulcers and pressure sores, pose significant challenges in modern healthcare due to their prolonged healing times and susceptibility to infections. This study aims to engineer a bilayered wound dressing (BLWD) composed of soy protein isolate/collagen with the ability to release Cinnamaldehyde, Artemisia absinthium (AA), and oxygen. Cinnamaldehyde, magnesium peroxide (MgO2), and AA extract were encapsulated. Nanoparticles were evaluated using scanning electron microscopy (SEM), dynamic light scattering, and ZETA potential tests. Swelling, degradation, water vapor penetration, tensile, MTT, SEM, oxygen release, AA extract release, and antibacterial properties were performed. An in vivo study was carried out to assess the final wound dressing under Hematoxiline&Eosin and Masson trichrome staining analysis and compared to a commercial product. According to the results, the synthesized nanoparticles had an average diameter of about 20 nm with a zeta potential in the range of -20 to -30 mV. The layers had uniform and dense surfaces. The maximum swelling and degradation of the dressing was about 130 and 13% respectively. Generally, better mechanical properties were observed in BLWD than in the single-layer case. More than 90% biocompatibility for the wound dressing was reported. The BLWD could inhibit the growth of Gram-positive and Gram-negative microorganisms. Histopathological analysis showed an acceptable wound-healing property. To sum up, the engineered wound dressing can be a good candidate for more clinical trials.

Asunto(s)

Acroleína , Alginatos , Artemisia absinthium , Vendajes , Materiales Biocompatibles , Colágeno , Proteínas de Soja , Cicatrización de Heridas , Animales , Ratas , Acroleína/análogos & derivados , Acroleína/química , Acroleína/farmacología , Alginatos/química , Antibacterianos/farmacología , Antibacterianos/química , Antibacterianos/síntesis química , Artemisia absinthium/química , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Colágeno/química , Escherichia coli/efectos de los fármacos , Ensayo de Materiales , Pruebas de Sensibilidad Microbiana , Oxígeno/química , Tamaño de la Partícula , Proteínas de Soja/química , Cicatrización de Heridas/efectos de los fármacos

RESUMEN

Developing bifunctional innovative food packaging for maintaining and monitoring food freshness is crucial for food safety. Here, we prepared tannic acid cinnamaldehyde nanoemulsions through self-assembly and ionic cross-linking between the natural emulsifiers tannic acid and cinnamaldehyde, and were incorporated into chitosan as a protective outer layer. Sodium alginate anchored with alizarin was employed as the sensing inner layer. A pH-sensitive bilayer film integrating real-time monitoring and maintenance of food fresh food freshness was designed using layer-by-layer assembly (LBL) technology. The prepared bilayer film exhibited 100 % UV protection, >99 % antimicrobial effect, and 94.86 % and 97.91 % clearance rates for DPPH and ABTS free radicals, respectively. In addition, the bilayer film exhibited high biosafety and sensitive, reversible, and rapid response to pH/NH3. Shrimp preservation experiments showed that the smart bilayer film could effectively slow down the growth of microorganisms on the surface of shrimp, extend the freshness period of shrimp, and could monitor the freshness of shrimp in real-time through color changes. In conclusion, the prepared SL-CCT bilayer film has excellent potential for food preservation and freshness monitoring, providing a new perspective for design and development of multifunctional smart food packaging films.

Asunto(s)

Alginatos , Quitosano , Embalaje de Alimentos , Quitosano/química , Alginatos/química , Embalaje de Alimentos/métodos , Animales , Penaeidae/química , Taninos/química , Conservación de Alimentos/métodos , Concentración de Iones de Hidrógeno , Acroleína/análogos & derivados , Acroleína/química , Acroleína/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Picratos/química , Compuestos de Bifenilo/química , Rayos Ultravioleta , Ácidos Sulfónicos , Benzotiazoles

RESUMEN

Patients with impaired immune systems are particularly vulnerable to infections. With the increasing number of immunocompromised patients, it becomes necessary to design studies that evaluate the effects of toxic contaminants that are a part of our daily lives. Simultaneously, the management of these toxic components also becomes essential. Therefore, the present study evaluated the possible protective role of cinnamaldehyde (Cin) against tenuazonic acid-induced mycotoxicosis in the immunosuppressed murine model. Tenuazonic acid (TeA), a toxin usually produced by Alternaria species, is a common contaminant in tomato and tomato-based products. Evaluating the potential toxicity of a hazardous chemical necessitates the use of in vitro, in vivo, and in silico methods. Here, the immunomodulatory effect of TeA was assessed in vitro using mouse splenocytes. In silico docking was carried out for the tumour markers of eight organs and TeA. The haematological, histopathological, and biochemical aspects were analysed in vivo. The sub-chronic intoxication of mice with TeA showed elevated malondialdehyde, reduced catalase, and superoxide dismutase production, along with abnormal levels of aspartate aminotransferase and alanine transaminase. The treatment with Cin prevented TeA-induced alterations of antioxidant defense enzyme activities and significantly forbade TeA-induced organ damage, showing therapeutic effects and toxicity reduction in TeA-induced mycotoxicosis.

Asunto(s)

Acroleína , Micotoxicosis , Ácido Tenuazónico , Animales , Acroleína/análogos & derivados , Acroleína/toxicidad , Acroleína/farmacología , Acroleína/química , Ratones , Ácido Tenuazónico/farmacología , Micotoxicosis/prevención & control , Micotoxicosis/tratamiento farmacológico , Modelos Animales de Enfermedad , Superóxido Dismutasa/metabolismo , Catalasa/metabolismo , Masculino , Huésped Inmunocomprometido , Malondialdehído/metabolismo , Simulación del Acoplamiento Molecular , Alternaria , Antioxidantes/farmacología , Antioxidantes/metabolismo

RESUMEN

Oleum cinnamomi (OCM) is a volatile component of the Cinnamomum cassia Presl in the Lauraceae family, which displays broad-spectrum antibacterial properties. It has been found that OCM has a significant inhibitory effect against Cutibacterium acnes (C. acnes), but the precise target and molecular mechanism are still not fully understood. In this study, the antibacterial activity of OCM against C. acnes and its potential effect on cell membranes were elucidated. Metabolomics methods were used to reveal metabolic pathways, and proteomics was used to explore the targets of OCM inhibiting C. acnes. The yield of the OCM was 3.3% (w/w). A total of 19 compounds were identified, representing 96.213% of the total OCM composition, with the major constituents being phenylpropanoids (36.84%), sesquiterpenoids (26.32%), and monoterpenoids (15.79%). The main component identified was trans-cinnamaldehyde (85.308%). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of OCM on C. acnes were 60 µg/mL and 180 µg/mL, respectively. The modified proteomics results indicate that cinnamaldehyde was the main bioactive ingredient within OCM, which covalently modifies the ABC transporter adenosine triphosphate (ATP)-binding protein and nicotinamide adenine dinucleotide (NADH)-quinone oxidoreductase, hindering the amino acid transport process, and disrupting the balance between NADH and nicotinamide adenine dinucleoside phosphorus (NAD+), thereby hindering energy metabolism. We have reported for the first time that OCM exerts an antibacterial effect by covalent binding of cinnamaldehyde to target proteins, providing potential and interesting targets to explore new control strategies for gram-positive anaerobic bacteria.

Asunto(s)

Antibacterianos , Antibacterianos/farmacología , Antibacterianos/química , Pruebas de Sensibilidad Microbiana , Propionibacteriaceae/efectos de los fármacos , Extractos Vegetales/farmacología , Extractos Vegetales/química , Proteómica/métodos , Acroleína/análogos & derivados , Acroleína/farmacología , Acroleína/química , Metabolómica/métodos

RESUMEN

Most of postharvest agricultural produces are perishable due to microorganisms infections and physiological change. Herein, one kind of multifunctional coating film of SC-ECCNPs was developed by incorporating organic nanoparticles of ECCNPs into starch/carboxymethylcellulose (SC) to prolong shelf life of food with excellent performances. The SC-ECCNPs coating was prepared with starch and sodium carboxymethylcellulose as film substrate (SC) to incorporate with organic nanoparticles of ECCNPs formed by integrating epigallocatechin-3-gallate (EGCG), cysteine (Cys), and cinnamaldehyde (CA). The incorporation of ECCNPs improves the UV-resistance and physical properties of SC-ECCNPs coating and also endows it with excellent antioxidative and broad-spectrum antibacterial activity. The application possibilities of SC-ECCNPs coating were explored with strawberries and oranges as samples, validating that the SC-ECCNPs coating can prolong the shelf life of fruits at room temperature. The biosafety of the coating was further confirmed with hemolysis and MTT experiments. The SC-ECCNPs coating film was prepared with natural substrates via a simple and green method. The investigation provides an instructive way for developing advanced packaging materials with high performances.

Asunto(s)

Antibacterianos , Carboximetilcelulosa de Sodio , Nanopartículas , Almidón , Almidón/química , Carboximetilcelulosa de Sodio/química , Nanopartículas/química , Antibacterianos/química , Antibacterianos/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Conservación de Alimentos/métodos , Embalaje de Alimentos/métodos , Frutas/química , Acroleína/análogos & derivados , Acroleína/química

RESUMEN

Cancer incidence is increasing globally, presenting a growing public health challenge. While anticancer drugs are crucial in treatment, their limitations, including poor targeting ability and high toxicity, hinder effectiveness and patient safety, requiring relentless scientific research and technological advancements to develop safer and more effective therapeutics. Cinnamaldehyde (CA), an active compound derived from the natural plant cinnamon, has garnered attention in pharmacological research due to its diverse therapeutic applications. CA has potential in treating a wide array of conditions, including cardiovascular diseases, diabetes, inflammatory disorders and various forms of cancer. The present review comprehensively summarizes the physicochemical and pharmacokinetic profiles of CA, and delves into the latest advancements in elucidating its potential mechanisms and targets across various cancer types. CA and its derivatives have antitumor effects, which encompass inhibiting cell proliferation, arresting the cell cycle, inducing apoptosis, limiting cell migration and invasion, and suppressing angiogenesis. Additionally, the present review explores targeted formulations of CA, laying a scientific foundation for further exploration of its implications in cancer prevention and treatment strategies.

Asunto(s)

Acroleína , Antineoplásicos , Neoplasias , Acroleína/análogos & derivados , Acroleína/química , Acroleína/farmacología , Acroleína/uso terapéutico , Humanos , Neoplasias/tratamiento farmacológico , Neoplasias/metabolismo , Animales , Antineoplásicos/uso terapéutico , Antineoplásicos/farmacología , Antineoplásicos/química , Antineoplásicos/farmacocinética , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos

RESUMEN

Biocatalysis is becoming a powerful and sustainable alternative for asymmetric catalysis. However, enzymes are often restricted to metabolic and less complex reactivities. This can be addressed by protein engineering, such as incorporating new-to-nature functional groups into proteins through the so-called expansion of the genetic code to produce artificial enzymes. Selecting a suitable protein scaffold is a challenging task that plays a key role in designing artificial enzymes. In this work, we explored different protein scaffolds for an abiological model of iminium-ion catalysis, Michael addition of nitromethane into E-cinnamaldehyde. We studied scaffolds looking for open hydrophobic pockets and enzymes with described binding sites for the targeted substrate. The proteins were expressed and variants harboring functional amine groups - lysine, p-aminophenylalanine, or N6-(D-prolyl)-L-lysine - were analyzed for the model reaction. Among the newly identified scaffolds, a thermophilic ene-reductase from Thermoanaerobacter pseudethanolicus was shown to be the most promising biomolecular scaffold for this reaction.

Asunto(s)

Biocatálisis , Iminas , Iminas/química , Iminas/metabolismo , Ingeniería de Proteínas , Thermoanaerobacter/enzimología , Acroleína/química , Acroleína/análogos & derivados , Acroleína/metabolismo , Modelos Moleculares

RESUMEN

Acrolein (ACR), methylglyoxal (MGO), and glyoxal (GO) are a class of reactive carbonyl species (RCS), which play a crucial role in the pathogenesis of chronic and age-related diseases. Here, we explored a new RCS inhibitor (theanine, THE) and investigated its capture capacity on RCS in vivo by human experiments. After proving that theanine could efficiently capture ACR instead of MGO/GO by forming adducts under simulated physiological conditions, we further detected the ACR/MGO/GO adducts of theanine in the human urine samples after consumption of theanine capsules (200 and 400 mg) or green tea (4 cups, containing 200 mg of theanine) by using ultraperformance liquid chromatography-time-of-flight-high-resolution mass spectrometry. Quantitative assays revealed that THE-ACR, THE-2ACR-1, THE-MGO, and THE-GO were formed in a dose-dependent manner in the theanine capsule groups; the maximum value of the adducts of theanine was also tested. Furthermore, besides the RCS adducts of theanine, the RCS adducts of catechins could also be detected in the drinking tea group. Whereas, metabolite profile analysis showed that theanine could better capture RCS produced in the renal metabolic pathway than catechins. Our findings indicated that theanine could reduce RCS in the body in two ways: as a pure component or contained in tea leaves.

Asunto(s)

Glutamatos , Glioxal , Piruvaldehído , Té , Humanos , Té/química , Glutamatos/metabolismo , Glutamatos/análisis , Masculino , Piruvaldehído/metabolismo , Piruvaldehído/química , Glioxal/metabolismo , Glioxal/química , Adulto , Acroleína/metabolismo , Acroleína/química , Cápsulas/química , Camellia sinensis/química , Camellia sinensis/metabolismo , Femenino , Adulto Joven , Extractos Vegetales/química , Extractos Vegetales/metabolismo , Extractos Vegetales/administración & dosificación , Cromatografía Líquida de Alta Presión

RESUMEN

As cancer continues to rise globally, there is growing interest in discovering novel methods for prevention and treatment. Due to the limitations of traditional cancer therapies, there has been a growing emphasis on investigating herbal remedies and exploring their potential synergistic effects when combined with chemotherapy drugs. Cinnamaldehyde, derived from cinnamon, has gained significant attention for its potential role in cancer prevention and treatment. Extensive research has demonstrated that cinnamaldehyde exhibits promising anticancer properties by modulating various cellular processes involved in tumor growth and progression. However, challenges and unanswered questions remain regarding the precise mechanisms for its effective use as an anticancer agent. This article aims to explore the multifaceted effects of cinnamaldehyde on cancer cells and shed light on these existing issues. Cinnamaldehyde has diverse anti-cancer mechanisms, including inducing apoptosis by activating caspases and damaging mitochondrial function, inhibiting tumor angiogenesis, anti-proliferation, anti-inflammatory and antioxidant. In addition, cinnamaldehyde also acts as a reactive oxygen species scavenger, reducing oxidative stress and preventing DNA damage and genomic instability. This article emphasizes the promising therapeutic potential of cinnamaldehyde in cancer treatment and underscores the need for future research to unlock novel mechanisms and strategies for combating cancer. By providing valuable insights into the role and mechanism of cinnamaldehyde in cancer, this comprehensive understanding paves the way for its potential as a novel therapeutic agent. Overall, cinnamaldehyde holds great promise as an anticancer agent, and its comprehensive exploration in this article highlights its potential as a valuable addition to cancer treatment options.

Asunto(s)

Acroleína , Neoplasias , Acroleína/análogos & derivados , Acroleína/farmacología , Acroleína/química , Humanos , Neoplasias/tratamiento farmacológico , Neoplasias/metabolismo , Animales , Apoptosis/efectos de los fármacos , Antineoplásicos/farmacología , Antineoplásicos/uso terapéutico , Daño del ADN/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo

RESUMEN

The efficacy of DNA-damaging agents, such as the topoisomerase I inhibitor SN38, is often compromised by the robust DNA repair mechanisms in tumor cells, notably homologous recombination (HR) repair. Addressing this challenge, we introduce a novel nano-strategy utilizing binary tumor-killing mechanisms to enhance the therapeutic impact of DNA damage and mitochondrial dysfunction in cancer treatment. Our approach employs a synergistic drug pair comprising SN38 and the BET inhibitor JQ-1. We synthesized two prodrugs by conjugating linoleic acid (LA) to SN38 and JQ-1 via a cinnamaldehyde thioacetal (CT) bond, facilitating co-delivery. These prodrugs co-assemble into a nanostructure, referred to as SJNP, in an optimal synergistic ratio. SJNP was validated for its efficacy at both the cellular and tissue levels, where it primarily disrupts the transcription factor protein BRD4. This disruption leads to downregulation of BRCA1 and RAD51, impairing the HR process and exacerbating DNA damage. Additionally, SJNP releases cinnamaldehyde (CA) upon CT linkage cleavage, elevating intracellular ROS levels in a self-amplifying manner and inducing ROS-mediated mitochondrial dysfunction. Our results indicate that SJNP effectively targets murine triple-negative breast cancer (TNBC) with minimal adverse toxicity, showcasing its potential as a formidable opponent in the fight against cancer.

Asunto(s)

Acroleína , Camptotecina , Sistemas de Liberación de Medicamentos , Nanopartículas , Neoplasias de la Mama Triple Negativas , Neoplasias de la Mama Triple Negativas/tratamiento farmacológico , Nanopartículas/administración & dosificación , Nanopartículas/química , Animales , Humanos , Femenino , Línea Celular Tumoral , Acroleína/análogos & derivados , Acroleína/administración & dosificación , Acroleína/química , Camptotecina/análogos & derivados , Camptotecina/administración & dosificación , Camptotecina/uso terapéutico , Camptotecina/farmacología , Profármacos/administración & dosificación , Profármacos/uso terapéutico , Ácido Linoleico/química , Ácido Linoleico/administración & dosificación , Triazoles/administración & dosificación , Triazoles/farmacología , Triazoles/química , Daño del ADN/efectos de los fármacos , Antineoplásicos/administración & dosificación , Antineoplásicos/uso terapéutico , Antineoplásicos/farmacología , Ratones Desnudos , Ratones , Proteínas de Ciclo Celular/metabolismo , Factores de Transcripción/metabolismo , Inhibidores de Topoisomerasa I/administración & dosificación , Proteínas que Contienen Bromodominio , Azepinas

RESUMEN

In this study, a novel adsorbent, A-PEI/CS-Cu2+, was developed by crosslinking polyethyleneimine/chitosan hydrogel with acrolein and loading it with copper ions. The adsorption process of A-PEI/CS-Cu2+ on the anionic dye acid yellow 36 (AY36) was investigated by kinetic, isothermal and thermodynamic modeling. It was noteworthy that A-PEI/CS-Cu2+ exhibited rapid adsorption with a 90 % removal rate achieved within just 5 min, which was much faster than the adsorption rate of A-PEI/CS without load of copper ions and showed its potential for rapid adsorption applications. The maximum adsorption capacity for AY36 could reach up to 3114 mg g-1. In addition, the high concentration of saline wastewater was found to have almost no effect on the adsorption reaction in the salt effect test experiment. In five desorption-regeneration cycle experiments, the sample exhibited good recyclability and regeneration performance. The driving force of the adsorption process mainly originated from the electrostatic interaction, hydrogen bonding, and intermolecular interaction, in which the addition of copper ions led to the enhancement of the electrostatic interaction and chelation between A-PEI/CS-Cu2+ and AY36. Overall, the findings suggest the excellent potential of A-PEI/CS-Cu2+ for rapid and efficient adsorption, as well as its suitability for practical applications in wastewater treatment.

Asunto(s)

Acroleína , Quitosano , Cobre , Hidrogeles , Polietileneimina , Contaminantes Químicos del Agua , Quitosano/química , Cobre/química , Polietileneimina/química , Adsorción , Hidrogeles/química , Contaminantes Químicos del Agua/química , Cinética , Acroleína/química , Compuestos Azo/química , Termodinámica , Aniones/química , Purificación del Agua/métodos , Colorantes/química , Aguas Residuales/química , Iones/química , Concentración de Iones de Hidrógeno

RESUMEN

Sodium alginate (SA) is widely used in the food, biomedical, and chemical industries due to its biocompatibility, biodegradability, and excellent film-forming properties. This article introduces a simple method for preparing uniform alginate-based packaging materials with exceptional properties for fruit preservation. The alginate was uniformly crosslinked by gradually releasing calcium ions triggered by the sustained hydrolysis of gluconolactone (GDL). A cinnamaldehyde (CA) emulsion, stabilized by xanthan without the use of traditional surfactants, was tightly incorporated into the alginate film to enhance its antimicrobial, antioxidant, and UV shielding properties. The alginate-based film effectively blocked ultraviolet rays in the range of 400-200 nm, while allowing for a visible light transmittance of up to 70 %. Additionally, it showed an increased water contact angle and decreased water vapor permeability. The alginate-based film was also employed in the preparation of coated paper through the commonly used coating process in the papermaking industry. The alginate-based material displayed excellent antioxidant properties and antimicrobial activity against Escherichia coli, Staphylococcus aureus and Botrytis cinerea, successfully extending the shelf life of strawberries to 7 days at room temperature. This low-cost and facile method has the potential to drive advancements in the food and biomedical fields by tightly incorporating active oil onto a wide range of biomacromolecule substrates.

Asunto(s)

Acroleína , Alginatos , Antioxidantes , Frutas , Alginatos/química , Frutas/química , Acroleína/análogos & derivados , Acroleína/química , Acroleína/farmacología , Antioxidantes/farmacología , Antioxidantes/química , Conservación de Alimentos/métodos , Embalaje de Alimentos/métodos , Antiinfecciosos/farmacología , Antiinfecciosos/química , Escherichia coli/efectos de los fármacos

RESUMEN

Addressing the intertwined challenges of antimicrobial resistance and impaired wound healing in diabetic patients, an oil/water emulsion-based nano-ointment integrating phenylpropanoids-Eugenol and Cinnamaldehyde-with positively-charged silver nanoparticles was synthesized. The process began with the synthesis and characterization of nano-silver, aimed at ensuring the effectiveness and safety of the nanoparticles in biological applications. Subsequent experiments determined the minimum inhibitory concentration (MIC) against pathogens such as Streptococcus aureus, Pseudomonas aeruginosa and Candida albicans. These MIC values of all three active leads guided the strategic formulation of an ointment base, which effectively integrated the bioactive components. Evaluations of this nano-ointment revealed enhanced antimicrobial activity against both clinical and reference bacterial strains and it maintained stability after freeze-thaw cycles. Furthermore, the ointment demonstrated superior in-vitro diabetic wound healing capabilities and significantly promoted angiogenesis, as shown by enhanced blood vessel formation in the Chorioallantoic Membrane assay. These findings underscore the formulation's therapeutic potential, marking a significant advance in the use of nanotechnology for topical wound care.

Asunto(s)

Nanopartículas del Metal , Pruebas de Sensibilidad Microbiana , Pomadas , Plata , Cicatrización de Heridas , Plata/administración & dosificación , Plata/química , Plata/farmacología , Cicatrización de Heridas/efectos de los fármacos , Nanopartículas del Metal/química , Nanopartículas del Metal/administración & dosificación , Animales , Acroleína/análogos & derivados , Acroleína/administración & dosificación , Acroleína/farmacología , Acroleína/química , Candida albicans/efectos de los fármacos , Antiinfecciosos/administración & dosificación , Antiinfecciosos/farmacología , Pseudomonas aeruginosa/efectos de los fármacos , Administración Tópica , Humanos , Antibacterianos/administración & dosificación , Antibacterianos/farmacología , Staphylococcus aureus/efectos de los fármacos

RESUMEN

Ferroptosis is a pattern of cell death caused by iron-dependent accumulation of lipid peroxides and is closely associated with the occurrence and development of multiple diseases. Acrolein (ACR), one of the final metabolites of lipid peroxidation, is a reactive carbonyl species with strong biotoxicity. Effective detection of ACR is important for understanding its role in the progression of ferroptosis and studying the specific mechanisms of ferroptosis-mediated diseases. However, visualization detection of ACR during ferroptosis has not yet been reported. In this work, the first ratiometric fluorescent probe (HBT-SH) based on 2-(2'-hydroxyphenyl) benzothiazole (HBT) was designed for tracing endogenous ACR with an unprecedented regiospecific ACR-induced intramolecular cyclization strategy, which employs 2-aminoethanethiol as an ACR-selective recognition receptor. The experimental results showed that HBT-SH has excellent selectivity, high sensitivity (LOD = 0.26 µM) and good biocompatibility. More importantly, the upregulation of ACR levels was observed during ferroptosis in HeLa cells and zebrafish, indicating that ACR may be a specific active molecule that plays an essential biological role during ferroptosis or may serve as a potential marker of ferroptosis, which has great significance for studying the pathological process and treatment options of ferroptosis-related diseases.

Asunto(s)

Acroleína , Ferroptosis , Colorantes Fluorescentes , Pez Cebra , Ferroptosis/efectos de los fármacos , Acroleína/química , Acroleína/metabolismo , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Humanos , Células HeLa , Animales , Regulación hacia Arriba/efectos de los fármacos , Imagen Óptica , Estructura Molecular