RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: The Chinese traditional medicine frankincense, which can promote blood circulation, is often used to treat skin lesions, including frostbite. AIM OF THE STUDY: To explore the properties of frankincense oil extract (FOE) and its active ingredients and their effect on frostbite wound recovery as an approach to understand the mechanism associated with microcirculation-improvement therapy. MATERIALS AND METHODS: The microcirculation-improving effects of FOE and its active ingredients were evaluated using liquid nitrogen-induced frostbite animal models. The rewarming capacity of FOE on the skin was determined through infrared detection, and frostbite wound healing was evaluated following haematoxylin and eosin (H&E) staining and fibre analysis. Moreover, related factors were examined to determine the anti-apoptotic, anti-inflammatory, and microcirculatory properties of FOE and its active ingredients on affected tissue in the context of frostbite. RESULTS: FOE and its active ingredients rapidly rewarmed wound tissue after frostbite by increasing the temperature. Moreover, these treatments improved wound healing and restored skin structure through collagen and elastin fibre remodelling. In addition, they exerted anti-apoptotic effects by decreasing the number of apoptotic cells, reducing caspase-3 expression, and eliciting anti-inflammatory effects by decreasing COX-2 and ß-catenin expression. They also improved microcirculatory disorders by decreasing HIF-1α expression and increasing CD31 expression. CONCLUSIONS: FOE and its active components can effectively treat frostbite by enhancing microcirculation, inhibiting the infiltration of inflammatory cells, decreasing cell apoptosis, and exerting antinociceptive effects. These findings highlight FOE as a new treatment option for frostbite, providing patients with an effective therapeutic strategy.

Asunto(s)

Congelación de Extremidades , Microcirculación , Cicatrización de Heridas , Congelación de Extremidades/tratamiento farmacológico , Animales , Microcirculación/efectos de los fármacos , Masculino , Cicatrización de Heridas/efectos de los fármacos , Piel/efectos de los fármacos , Piel/irrigación sanguínea , Piel/patología , Apoptosis/efectos de los fármacos , Ratas , Modelos Animales de Enfermedad , Ratones , Administración Tópica , Ratas Sprague-Dawley , Aceites de Plantas/farmacología , Aceites de Plantas/uso terapéutico , Extractos Vegetales/farmacología

RESUMEN

Aquatic products are highly susceptible to spoilage, and preparing composite edible film with essential oil is an effective solution. In this study, composite edible films were prepared using perilla essential oil (PEO)-glycerol monolaurate emulsions incorporated with chitosan and nisin, and the film formulation was optimized by response surface methodology. These films were applied to ready-to-eat fish balls and evaluated over a period of 12 days. The films with the highest inhibition rate against Staphylococcus aureus were acquired using a polymer composition of 6 µL/mL PEO, 18.4 µg/mL glycerol monolaurate, 14.2 mg/mL chitosan, and 11.0 µg/mL nisin. The fish balls coated with the optimal edible film showed minimal changes in appearance during storage and significantly reduced total bacterial counts and total volatile basic nitrogen compared to the control groups. This work indicated that the composite edible films containing essential oils possess ideal properties as antimicrobial packaging materials for aquatic foods.

Asunto(s)

Antibacterianos , Quitosano , Películas Comestibles , Emulsiones , Embalaje de Alimentos , Lauratos , Monoglicéridos , Nisina , Aceites Volátiles , Staphylococcus aureus , Nisina/farmacología , Nisina/química , Aceites Volátiles/química , Aceites Volátiles/farmacología , Lauratos/química , Lauratos/farmacología , Embalaje de Alimentos/instrumentación , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo , Emulsiones/química , Quitosano/química , Quitosano/farmacología , Monoglicéridos/química , Monoglicéridos/farmacología , Antibacterianos/farmacología , Antibacterianos/química , Aceites de Plantas/química , Aceites de Plantas/farmacología , Perilla/química

RESUMEN

Perilla essential oil (PLEO) offers benefits for food preservation and healthcare, yet its instability restricts its applications. In this study, chitosan (CS) and TiO2 used to prepare composite particles. TiO2, after being modified with sodium laurate (SL), was successfully introduced at 0.1 %-3 % into the CS matrix. The resulting CS-SL-TiO2 composite particles can be formed by intertwining and rearranging through intramolecular and intermolecular interactions, and form an O/W interface with stability and viscoelasticity. The Pickering emulsions stabilized by these particles exhibit non-Newtonian pseudoplastic behavior, shear-thinning properties, and slow-release characteristics, along with antibacterial activity. Emulsions with 0.5 % and 1 % CS-SL-TiO2 composites demonstrated superior antibacterial effects against Escherichia coli and Staphylococcus aureus. The study revealed that all emulsions undergo Fickian diffusion and a sustained release of PLEO, with the Ritger-Peppas model best describing this release mechanism. The slow-release behaviors positively correlates with interfacial pressure, composite particle size, composite particle potential, composite contact angle, emulsion particle size and emulsion potential, but negatively correlates with diffusion rate, penetration rate, release kinetics and release rate. The findings lay groundwork for developing slow-release antimicrobial emulsions within polysaccharide matrices, showcasing promise for antimicrobial packaging solutions and enhanced food preservation techniques.

Asunto(s)

Antibacterianos , Quitosano , Emulsiones , Escherichia coli , Staphylococcus aureus , Titanio , Agua , Quitosano/química , Quitosano/farmacología , Titanio/química , Antibacterianos/química , Antibacterianos/farmacología , Staphylococcus aureus/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Agua/química , Aceites Volátiles/química , Aceites Volátiles/farmacología , Tamaño de la Partícula , Preparaciones de Acción Retardada/química , Aceites de Plantas/química , Aceites de Plantas/farmacología , Pruebas de Sensibilidad Microbiana , Liberación de Fármacos

RESUMEN

Mosquito-borne diseases, such as malaria, dengue fever, and the Zika virus, pose significant global health challenges, affecting millions annually. Due to increasing insecticide resistance, there is a growing interest in natural alternatives for mosquito control. Lemongrass essential oil, derived from Cymbopogon citratus, has shown promising repellent and larvicidal properties against various mosquito species. In this study, we investigated the larvicidal effect of lemongrass oil and its major compounds on Anopheles sinensis, the primary malaria vector in China. GC-MS analysis identified the major compounds of lemongrass oil as ( +)-citronellal (35.60%), geraniol (21.84%), and citronellol (13.88%). Lemongrass oil showed larvicidal activity against An. sinensis larvae, with an LC50 value of 119.20 ± 3.81 mg/L. Among the major components, citronellol had the lowest LC50 value of 42.76 ± 3.18 mg/L. Moreover, citronellol demonstrated inhibitory effects on acetylcholinesterase (AChE) activity in An. sinensis larvae, assessed by homogenizing larvae at different time points following treatment. Molecular docking studies further elucidated the interaction between citronellol and AChE, revealing the formation of hydrogen bonds and Pi-Sigma bonds. Aromatic amino acid residues such as Tyr71, Trp83, Tyr370, and Tyr374 played a pivotal role in these interactions. These findings may contribute to understanding lemongrass oil's larvicidal activity against An. sinensis and the mechanisms underlying these effects.

Asunto(s)

Monoterpenos Acíclicos , Anopheles , Inhibidores de la Colinesterasa , Insecticidas , Larva , Aceites Volátiles , Aceites de Plantas , Animales , Anopheles/efectos de los fármacos , Anopheles/enzimología , Larva/efectos de los fármacos , Insecticidas/farmacología , Insecticidas/química , Monoterpenos Acíclicos/farmacología , Aceites de Plantas/farmacología , Aceites de Plantas/química , Aceites Volátiles/farmacología , Aceites Volátiles/química , Inhibidores de la Colinesterasa/farmacología , Inhibidores de la Colinesterasa/química , Cymbopogon/química , Simulación del Acoplamiento Molecular , Terpenos/farmacología , Terpenos/química , Cromatografía de Gases y Espectrometría de Masas , China , Acetilcolinesterasa/metabolismo , Mosquitos Vectores/efectos de los fármacos , Monoterpenos/farmacología , Monoterpenos/química , Aldehídos/farmacología , Aldehídos/química

RESUMEN

INTRODUCTION: Essential oils are secondary metabolites of aromatic plants and are used in phytotherapy to treat various diseases. In the present study, eight selected essential oils - ajwain oil (Trachyspermum ammi L.), fennel oil (Foeniculum vulgare Mill. subsp. vulgare var. vulgare), thyme oil chemotype (ct.) thymol (Thymus vulgaris L.), tea tree oil (Melaleuca alternifolia Cheel.), oregano oil (Origanum vulgare L.), mountain savory oil (Satureja montana L.), lemongrass oil (Cymbopogon citratus (DC.) Stapf.) and eucalyptus oil (Eucalyptus globulus Labill.) -were examined for their antibacterial effect against Pasteurella (P.) multocida and Mannheimia (M.) haemolytica isolates from deep nasopharyngeal swab samples of fattening calves using agar diffusion and microdilution. All eight essential oils were effective against the tested isolates. Lemongrass oil proved to be the most potent of all eight essential oils, while fennel oil was only weakly effective. Different antimicrobial effects were observed between the two research methods. The effectiveness of ajwain, thyme, oregano and mountain savory oils was comparable in agar diffusion. However, this could not be reproduced using the microdilution method. P. multocida was found to be more sensitive to all essential oils tested than M. haemolytica. This study shows that the tested essential oils have antimicrobial in-vitro effects on P. multocida and M. haemolytica isolates and that the examination method is associated with the test result.

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INTRODUCTION: Les huiles essentielles sont des métabolites secondaires de plantes aromatiques et sont utilisées en phytothérapie pour le traitement de différentes maladies. Dans la présente étude, huit huiles essentielles sélectionnées ­ huile d'ajowan (Trachyspermum ammi L.), huile de fenouil (Foeniculum vulgare Mill. subsp. vulgare var. vulgare), huile de thym chémotype (ct.) thymol (Thymus vulgaris L.), huile d'arbre à thé (Melaleuca alternifolia Cheel.), huile d'origan (Origanum vulgare L.), huile de sarriette de montagne (Satureja montana L. ), huile de citronnelle (Cymbopogon citratus (DC.) Stapf.) et huile d'eucalyptus (Eucalyptus globulus Labill.) ­ ont été étudiées par diffusion sur gélose et microdilution pour leur effet antibactérien sur des isolats de Pasteurella (P.) multocida et de Mannheimia (M.) haemolytica provenant d'échantillons d'écouvillons nasaux profonds de veaux d'engraissement. Les huit huiles essentielles se sont révélées efficaces sur les isolats testés. L'huile de citronnelle s'est avérée être la plus puissante des huit huiles essentielles, tandis que l'huile de fenouil n'était que faiblement efficace. Des effets différents ont été observés entre les deux méthodes de recherche utilisées. Par exemple, l'efficacité des huiles d'ajowan, de thym, d'origan et de sarriette de montagne était comparable dans la diffusion sur gélose. Cependant, cela n>a pas pu être reproduit avec la méthode de microdilution. P. multocida s'est révélée plus sensible que M. haemolytica à toutes les huiles essentielles testées. Cette étude montre premièrement que les huiles essentielles testées ont une efficacité antimicrobienne in vitro sur des isolats cliniques de P. multocida et de M. haemolytica. Deuxièmement, elle montre que la méthode d'examen est associée au résultat du test.

Asunto(s)

Mannheimia haemolytica , Aceites Volátiles , Pasteurella multocida , Animales , Aceites Volátiles/farmacología , Pasteurella multocida/efectos de los fármacos , Bovinos , Mannheimia haemolytica/efectos de los fármacos , Antibacterianos/farmacología , Pruebas de Sensibilidad Microbiana , Nasofaringe/microbiología , Aceites de Plantas/farmacología

RESUMEN

Mentha arvensis is an herbaceous plant commonly known as peppermint or Japanese mint. This study investigated the toxic potential and repellent efficacy of M. arvensis essential oil (MaEO) at varying concentrations (15.625-250 mg/mL) in Nauphoeta cinerea, along with its impact on biochemical parameters in N. cinerea. The potential of the major compounds as a new analgesic target was investigated using molecular docking. The essential oil was analyzed by gas Chromatography-mass spectrometry (GC-MS) and the toxic potential, repellent property, and changes in lipid peroxidation (LPO) levels were evaluated as markers of oxidative stress. GC-MS results revealed that the main components were oxygenated monoterpenes such as menthol (71.31%), mentone (13.34%) and isomentone (5.35%). MaEO significantly reduced lipid peroxidation (LPO), the levels of non-protein thiols and iron(II) at the concentration of 125 mg/mL in N. cinerea. Furthermore, the major components, L-(-)-Menthol and menthone demonstrated high gastrointestinal absorption and high affinity with the target protein, suggesting possible links that contribute to the analgesic effect of MaEO.

Asunto(s)

Antioxidantes , Peroxidación de Lípido , Mentha , Aceites Volátiles , Mentha/química , Antioxidantes/farmacología , Antioxidantes/química , Aceites Volátiles/farmacología , Aceites Volátiles/química , Peroxidación de Lípido/efectos de los fármacos , Animales , Simulación del Acoplamiento Molecular , Estrés Oxidativo/efectos de los fármacos , Cromatografía de Gases y Espectrometría de Masas , Repelentes de Insectos/farmacología , Repelentes de Insectos/química , Aceites de Plantas/farmacología , Aceites de Plantas/química

RESUMEN

In vivo studies on the hazards of deep-fried foods were commonly done by feeding used-or heated-cooking oil to rats. The aim of this study was to determine the effect of feeding tempe deep-fried in palm, olive, and coconut oils and the used frying oil on the blood biochemical profile of laboratory rats. An in vivo randomized control group study with pre-test and post-test was conducted. This study included healthy male Sprague-Dawley rats aged 2-3 months and weighing 100-200 grams. After acclimatization, the rats were randomly assigned to seven groups, which were: (1) regular diet (control diet); (2) diet of tempe deep-fried in 5× used palm oil (Tempe-in-used-Po); (3) diet of tempe deep-fried in 5× used coconut oil (Tempe-in-used-Co); (4) diet of tempe deep-fried in 5× used olive oil (Tempe-in-used-Oo); (5) diet of 5× used palm oil (Used-Po); (6) diet of 5× used coconut oil (Used-Co); and (7) diet of 5× used olive oil (Used-Oo). Each rat received 15 grams of a treatment diet daily and blood samples were collected after four weeks for a complete blood count and serum biochemistry analysis. The results showed that the final body weight and the weight gain of Tempe-in-used-Po, Tempe-in-used-Co, Tempe-in-used-Oo group, and Used-Po groups increased significantly compared to the control, Used-Co, and Used-Oo groups. However, there was a significant increase in serum tumor necrosis factor-alpha (TNF-α) in the Used-Co and Used-Oo groups (p<0.05), suggesting the used oil's detrimental effect. The Used-Co and Used-Oo were the only two groups whose creatinine increased significantly (p<0.05). Subsequently, only the Used-Oo group had a significantly increased malondialdehyde (MDA) level compared to all groups (p<0.05). These results prove that the effect of feeding fried food differs from used oils. Feeding used oil did not reflect the consumption of fried foods as part of the whole diet and generally resulted in more harmful effects. This is the first study to report an in vivo rat feeding study of deep-fried tempe and the used oil as part of the diet.

Asunto(s)

Aceite de Coco , Culinaria , Creatinina , Malondialdehído , Aceite de Oliva , Ratas Sprague-Dawley , Factor de Necrosis Tumoral alfa , Animales , Masculino , Ratas , Factor de Necrosis Tumoral alfa/sangre , Factor de Necrosis Tumoral alfa/metabolismo , Creatinina/sangre , Malondialdehído/sangre , Malondialdehído/metabolismo , Aceite de Oliva/administración & dosificación , Aceite de Oliva/farmacología , Aceite de Palma/administración & dosificación , Aceite de Palma/farmacología , Aceite de Palma/química , Aceites de Plantas/farmacología , Aceites de Plantas/química

RESUMEN

Silver nanoparticles (AgNPs) synthesized from plant extracts have gained attention for their potential applications in biomedicine. Calotropis gigantea has been utilized to synthesize AgNPs, called AgNPs-LCg, and exhibit antibacterial activities against both Gram-positive and Gram-negative bacteria as well as antifungal. However, further enhancement of their antimicrobial properties is needed. The aim of this study was to synthesize AgNPs-LCg and to enhance their antimicrobial and antifungal activities through a hybrid green synthesis reaction using patchouli oil (PO), as well as to characterize the synthesized AgNPs-LCg. Optimization was conducted using the response surface method (RSM) with a central composite design (CCD). AgNPs-LCg were synthesized under optimal conditions and hybridized with different forms of PO-crude, distillation wastewater (hydrolate), and heavy and light fractions-resulting in PO-AgNPs-LCg, PH-AgNPs-LCg, LP-AgNPs-LCg, and HP-AgNPs-LCg, respectively. The samples were then tested for their antibacterial (both Gram-positive and Gram-negative bacteria) and antifungal activities. Our data indicated that all samples, including those with distillation wastewater, had enhanced antimicrobial activity. HP-AgNPs-LCg, however, had the highest efficacy; therefore, only HP-AgNPs-LCg proceeded to the characterization stage for comparison with AgNPs-LCg. UV-Vis spectrophotometry indicated surface plasmon resonance (SPR) peaks at 400 nm for AgNPs-LCg and 360 nm for HP-AgNPs-LCg. The Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the presence of O-H, N-H, and C-H groups in C. gigantea extract and AgNP samples. The smallest AgNPs-LCg were 56 nm, indicating successful RSM optimization. Scanning electron microscopy (SEM) analysis revealed spherical AgNPs-LCg and primarily cubic HP-AgNPs-LCg, with energy-dispersive X-ray spectroscopy (EDX) confirming silver's predominance. This study demonstrated that PO in any form significantly enhances the antimicrobial properties of AgNPs-LCg. The findings pave the way for the exploration of enhanced and environmentally sustainable antimicrobial agents, capitalizing on the natural resources found in Aceh Province, Indonesia.

Asunto(s)

Calotropis , Tecnología Química Verde , Nanopartículas del Metal , Pruebas de Sensibilidad Microbiana , Hojas de la Planta , Plata , Nanopartículas del Metal/química , Plata/química , Plata/farmacología , Tecnología Química Verde/métodos , Hojas de la Planta/química , Calotropis/química , Bacterias Grampositivas/efectos de los fármacos , Bacterias Gramnegativas/efectos de los fármacos , Extractos Vegetales/química , Extractos Vegetales/farmacología , Antiinfecciosos/farmacología , Antiinfecciosos/química , Antifúngicos/farmacología , Antifúngicos/química , Antifúngicos/síntesis química , Antibacterianos/farmacología , Antibacterianos/química , Antibacterianos/síntesis química , Aceites de Plantas/farmacología , Aceites de Plantas/química

RESUMEN

Tomato pomace, the main by-product of tomato processing, is also an underestimated source of many active substances. This study aimed to determine the possibility of using oil obtained from tomato pomace in a face cream formulation. The bacterial community structure, face skin biophysical parameters and protection against air pollution were examined after daily application of the cosmetic by volunteers. In the tomato pomace oil, the profile of fatty acids was determined by GC‒MS, and the profile of volatile compounds was determined using the HS-SPME technique. The dominant bioactive component in the oil was linoleic acid (63.6%), and among the volatile compounds, it was carvotanacetone (25.8%). The application of the cream with tomato pomace oil resulted in an increase in the dominant genera Staphylococcus, Anaerococcus and Cutibacterium in the epibiome, particularly beneficial Staphylococcus epidermidis, while limiting the growth of the potentially opportunistic pathogens Kocuria spp., Micrococcus spp., Veillonella spp., and Rothia spp. This study showed the usefulness of tomato pomace oil as a natural ingredient in skin care cosmetics, reducing skin inflammation, sensitivity and melanin level, with potential protective effects against air pollution and microbiome-balance properties. Tomato pomace, which is commonly considered waste after tomato processing, can be used in the development of new cosmetics and may additionally contribute to reducing environmental nuisance.

Asunto(s)

Microbiota , Solanum lycopersicum , Solanum lycopersicum/microbiología , Microbiota/efectos de los fármacos , Humanos , Femenino , Adulto , Compuestos Orgánicos Volátiles/análisis , Compuestos Orgánicos Volátiles/farmacología , Crema para la Piel/farmacología , Persona de Mediana Edad , Piel/microbiología , Piel/efectos de los fármacos , Bacterias/efectos de los fármacos , Bacterias/clasificación , Aceites de Plantas/farmacología , Masculino

RESUMEN

This study aimed to evaluate the toxicity of Piper hispidinervum essential oil (PHEO) against 11 Brazilian populations of Sitophilus zeamais (Coleoptera: Curculionidae). The effects of sublethal doses of PHEO on the behavior (walking and flying), respiration, and population growth (ri) of the insect populations were investigated. PHEO toxicity was determined through concentration-mortality bioassays, with mortality curves established using increasing PHEO concentrations ranging from 140.00 to 1000.00 µL kg-1. Behavior was evaluated based on walking distance, walking time, walking speed, walking time proportion, flight height, and flight takeoff success. Respiration was measured via the respiratory rate, while population growth (ri) was assessed through the instantaneous growth rate. All 11 populations of S. zeamais were susceptible to PHEO, showing no signs of resistance. The populations exhibited varying behavioral and physiological responses to sublethal exposure to PHEO, indicating different mitigation strategies. The results confirm that PHEO possesses insecticidal potential for controlling S. zeamais populations. However, the observed behavioral and physiological responses should be considered when establishing control measures in pest management programs for stored products.

Asunto(s)

Insecticidas , Aceites Volátiles , Piper , Gorgojos , Animales , Aceites Volátiles/farmacología , Aceites Volátiles/química , Gorgojos/efectos de los fármacos , Gorgojos/fisiología , Piper/química , Insecticidas/farmacología , Insecticidas/toxicidad , Conducta Animal/efectos de los fármacos , Aceites de Plantas/farmacología , Aceites de Plantas/química

RESUMEN

A nutritional approach could be a promising strategy to prevent or decrease the progression of neurodegenerative disorders such as Parkinson's disease (PD). The neuroprotective role of walnut oil (WO) was investigated in Drosophila melanogaster treated with rotenone (Rot), as a PD model, WO, or their combination, and compared to controls. WO reduced mortality and improved locomotor activity impairment after 3 and 7 days, induced by Rot. LC-MS analyses of fatty acid levels in Drosophila heads showed a significant increase in linolenic (ALA) and linoleic acid (LA) both in flies fed with the WO-enriched diet and in those treated with the association of WO with Rot. Flies supplemented with the WO diet showed an increase in brain dopamine (DA) level, while Rot treatment significantly depleted dopamine content; conversely, the association of Rot with WO did not modify DA content compared to controls. The greater intake of ALA and LA in the enriched diet enhanced their levels in Drosophila brain, suggesting a neuroprotective role of polyunsaturated fatty acids against Rot-induced neurotoxicity. The involvement of the dopaminergic system in the improvement of behavioral and biochemical parameters in Drosophila fed with WO is also suggested.

Asunto(s)

Modelos Animales de Enfermedad , Drosophila melanogaster , Juglans , Enfermedad de Parkinson , Aceites de Plantas , Animales , Drosophila melanogaster/efectos de los fármacos , Juglans/química , Enfermedad de Parkinson/tratamiento farmacológico , Enfermedad de Parkinson/metabolismo , Aceites de Plantas/farmacología , Aceites de Plantas/química , Dopamina/metabolismo , Rotenona , Encéfalo/metabolismo , Encéfalo/efectos de los fármacos , Fármacos Neuroprotectores/farmacología

RESUMEN

AIMS: Evaluate the in vitro efficacy of the essential oils derived from Aloysia citrodora (Verbenaceae), Cymbopogon winterianus (Poaceae), and Ocimum gratissimum (Lamiaceae) against Acanthamoeba polyphaga trophozoites. Additionally, microemulsions formulated with these essential oils, along with their major components, were analyzed. METHODS AND RESULTS: The prepared microemulsions were characterized using polarized light microscopy and rheological techniques. The amoebicidal activity was determined by measuring the inhibitory concentration (IC50). Flow cytometry was employed to detect membrane damage and alterations in trophozoites size. The results revealed transparent and thermodynamically stable microemulsions. The essential oil from O. gratissimum exhibited a lower IC50, with values of 280.66 and 47.28 µg ml-1 after 24 and 48 h, respectively. When microemulsions containing essential oils were tested, the IC50 values exhibited a reduction of over 80% after 24 h. Particularly, eugenol, a constituent of the O. gratissimum essential oil, displayed higher amoebicidal activity. The essential oils also caused damage to the cell membrane, resulting in the subsequent death of the trophozoites. CONCLUSIONS: The EOs of A. citrodora, C. winterianus, and O. gratissimum and their microemulsions showed antiparasitic effect against A. polyphaga trophozoites, representing promising alternatives for the treatment of diseases caused by this protozoan.

Asunto(s)

Acanthamoeba , Cymbopogon , Emulsiones , Ocimum , Aceites Volátiles , Trofozoítos , Verbenaceae , Aceites Volátiles/farmacología , Aceites Volátiles/química , Cymbopogon/química , Ocimum/química , Emulsiones/farmacología , Trofozoítos/efectos de los fármacos , Acanthamoeba/efectos de los fármacos , Verbenaceae/química , Amebicidas/farmacología , Aceites de Plantas/farmacología , Extractos Vegetales/farmacología

RESUMEN

Hazelnut oil cake (HOC) has the potential to be bioactive component source. Therefore, HOC was processed with a solid-state fermentation (SSF) by Aspergillus oryzae with two steps optimization: Plackett-Burman and Box-Behnken design. The variables were the initial moisture content (X1: 30-50%), incubation temperature (X2: 26-37 °C), and time (X3: 3-5 days), and the response was total peptide content (TPC). The fermented HOC (FHOC) was darker with higher protein, oil, and ash but lower carbohydrate content than HOC. The FHOC had 6.1% more essential amino acid and benzaldehyde comprised 48.8% of determined volatile compounds. Fermentation provided 14 times higher TPC (462.37 mg tryptone/g) and higher phenolic content as 3.5, 48, and 7 times in aqueous, methanolic, and 80% aqueous methanolic extract in FHOC, respectively. FHOC showed higher antioxidant as ABTS+ (75.61 µmol Trolox/g), DPPH (14.09 µmol Trolox/g), and OH (265 mg ascorbic acid/g) radical scavenging, and α-glucosidase inhibition, whereas HOC had more angiotensin converting enzyme inhibition. HOC showed better water absorption while FHOC had better oil absorption activity. Both cakes had similar foaming and emulsifying activity; however, FHOC produced more stable foams and emulsions. SSF at lab-scale yielded more bioactive component with better functionality in FHOC.

Asunto(s)

Antioxidantes , Aspergillus oryzae , Corylus , Fermentación , Aceites de Plantas , Aspergillus oryzae/metabolismo , Corylus/química , Antioxidantes/farmacología , Antioxidantes/química , Aceites de Plantas/química , Aceites de Plantas/farmacología , Fenoles/química

RESUMEN

α-tocopherol (α-T), ß-sitosterol (ß-S), canolol (CA), and sinapic acid (SA) are the four main endogenous lipid phytochemicals (LP) found in Brassica napus L. seed oil, which possess the bioactivity to prevent the risk of several chronic diseases via antioxidant-associated mechanisms. Discovering the enhancer effects or synergies between LP is valuable for resisting oxidative stress and improving health benefits. The objectives of this study were to identify a potentially efficacious LP combination by central composite design (CCD) and cellular antioxidant activity (CAA) and to investigate its protective effect and potential mechanisms against H2O2-induced oxidative damage in HepG2 cells. Our results indicated that the optimal concentration of LP combination was α-T 10 µM, ß-S 20 µM, SA 125 µM, and CA 125 µM, respectively, and its CAA value at the optimal condition was 10.782 µmol QE/100 g. At this concentration, LP combination exerted a greater amelioration effect on H2O2-induced HepG2 cell injury than either antioxidant (tea polyphenols or magnolol) alone. LP combination could reduce the cell apoptosis rate induced by H2O2, lowered to 10.06%, and could alleviate the degree of oxidative damage to cells (ROS↓), lipids (MDA↓), proteins (PC↓), and DNA (8-OHdG↓). Additionally, LP combination enhanced the antioxidant enzyme activities (SOD, CAT, GPX, and HO-1), as well as the T-AOC, and increased the GSH level in HepG2 cells. Furthermore, LP combination markedly upregulated the expression of Nrf2 and its associated antioxidant proteins. It also increased the expression levels of Nrf2 downstream antioxidant target gene (HO-1, SOD-1, MnSOD, CAT, GPX-1, and GPX-4) and downregulated the mRNA expression levels of Keap1. The oxidative-stress-induced formation of the Keap1/Nrf2 complex in the cytoplasm was significantly blocked by LP treatment. These results indicate that LP combination protected HepG2 cells from oxidative stress through a mechanism involving the activation of the Keap1/Nrf2/ARE signaling pathways.

Asunto(s)

Antioxidantes , Brassica napus , Peróxido de Hidrógeno , Proteína 1 Asociada A ECH Tipo Kelch , Factor 2 Relacionado con NF-E2 , Estrés Oxidativo , Fitoquímicos , Semillas , Transducción de Señal , Humanos , Células Hep G2 , Proteína 1 Asociada A ECH Tipo Kelch/metabolismo , Estrés Oxidativo/efectos de los fármacos , Factor 2 Relacionado con NF-E2/metabolismo , Transducción de Señal/efectos de los fármacos , Fitoquímicos/farmacología , Antioxidantes/farmacología , Semillas/química , Elementos de Respuesta Antioxidante/efectos de los fármacos , Aceites de Plantas/farmacología , Sitoesteroles/farmacología

RESUMEN

The current research was conducted to explore, for the first time, Tagetes erecta L. (family Asteraceae) fruits from northwest Iran in terms of the chemical composition of essential and fixed oils, their cytotoxic activities, and the inhibitory effect of essential oil on the PI3K/AKT/mTOR signaling pathway. The volatile oil was obtained through hydrodistillation (Clevenger apparatus). According to gas chromatography-mass spectrometry analysis, the essential oil was rich in cyclic monoterpenoids, 2-isopropyl-5-methyl-3-cyclohexen-1-one (19.99%), D-limonene (12.75%), terpinolene (11.64%) and also the saturated fatty acid palmitic acid (19.09%). Furthermore, the seeds of T. erecta were extracted using hexane by the maceration method. The analysis of fatty acid profile of the fixed oil by gas chromatography-flame ionization detector (GC-FID) demonstrated that the most predominant fatty acids in fixed oil were linoleic acid (59.53%), palmitic acid (13.70%), stearic acid (10.20%), and oleic acid (9.20%). The cytotoxic activity of essential oil, crude oil, and fraction A (obtained from fixed oil) were evaluated by using the MTT assay on MCF7 (human breast cancer cell line), PC3 (human prostate cancer cell line), and U87MG (human glioblastoma cell line). Finally, the effect of essential oil on inhibiting the PI3K/Akt/mTOR signaling pathway was evaluated using real-time PCR. The essential oil exhibited vigorous cytotoxic activity on the U87MG cell line, with an IC50 value of 32.65 µg/mL. Interestingly, the essential oil significantly inhibited the PI3K/AKT/mTOR cascade compared to the non-treated group. Our results suggest that the essential oil holds promise as an anticancer agent for glioblastoma cell lines. To the best of our knowledge, this study is the first to report on the profile of the essential oil of T. erecta fruits and its implications for targeting the PI3K/AKT/mTOR signaling pathway.

Asunto(s)

Frutas , Aceites Volátiles , Transducción de Señal , Tagetes , Humanos , Aceites Volátiles/farmacología , Aceites Volátiles/química , Transducción de Señal/efectos de los fármacos , Tagetes/química , Frutas/química , Línea Celular Tumoral , Irán , Proteínas Proto-Oncogénicas c-akt/metabolismo , Aceites de Plantas/farmacología , Aceites de Plantas/química , Serina-Treonina Quinasas TOR/metabolismo , Antineoplásicos Fitogénicos/farmacología , Antineoplásicos Fitogénicos/química , Fosfatidilinositol 3-Quinasas/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Proliferación Celular/efectos de los fármacos

RESUMEN

The term natural oil refers to a fixed (non-volatile) oil of animal or plant origin. These types of oils - in contrast to essential (volatile) oils, which are obtained by steam distillation methods of plant matter - are typically obtained from plant seeds and nuts by a mechanical pressing technique or solvent extraction. The natural movement in cosmetics of the 21st century has led to renewed interest in formulating skin care products with botanical ingredients. In this article, we discuss the benefits and caveats of natural oil treatments as moisturizing agents (as occlusives and emollients) as well as their utility in wound healing and treatment of skin diseases. We also address the paradoxical behaviour of natural oils in relation to barrier function and highlight the current state of our knowledge with respect to the use of natural oils in neonatal skin care. Finally, we provide a comparison of natural oils to conventional petroleum-based oils.

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Le terme huile naturelle fait référence à une huile fixe (non volatile) d'origine animale ou végétale. Ces types d'huiles, contrairement aux huiles essentielles (volatiles) qui sont obtenues par des méthodes de distillation à la vapeur de matières végétales, sont généralement obtenues à partir de graines et de noix de plantes par une technique de pressage mécanique ou d'extraction par solvant. Le mouvement naturel des cosmétiques du XXI siècle a suscité un regain d'intérêt pour la formulation de produits de soins pour la peau à base d'ingrédients botaniques. Dans cet article, nous examinons les avantages et les mises en garde des traitements à base d'huiles naturelles en tant qu'agents hydratants (comme occlusifs et émollients), ainsi que leur utilité dans la cicatrisation des plaies et le traitement des maladies de la peau. Nous abordons également le comportement paradoxal des huiles naturelles par rapport à la fonction barrière et mettons en évidence l'état actuel de nos connaissances en ce qui concerne l'utilisation des huiles naturelles dans les soins de la peau néonatale. Enfin, nous comparons les huiles naturelles aux huiles conventionnelles à base de pétrole.

Asunto(s)

Enfermedades de la Piel , Humanos , Enfermedades de la Piel/tratamiento farmacológico , Aceites de Plantas/farmacología , Aceites de Plantas/química , Aceites de Plantas/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos , Piel/efectos de los fármacos , Cosméticos

RESUMEN

PURPOSE: The flea Ctenocephalides felis (Siphonaptera: Pulicidae), parasitizes dogs and cats globally, acting as a vector for various pathogens affecting both animals and humans. Growing interest in environmentally friendly, plant-based products prompted this study. The aim of the study was to determine the chemical composition of essential oils (EOs) from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea, assessing their insecticidal and repellent properties, determining lethal concentrations (LC50 and LC90), and evaluating residual efficacy in vitro against Ctenocephalides felis felis. METHODS: Gas Chromatography with Flame Ionization Detector analyzed EO composition. In vitro tests involved preparing EO solutions at various concentrations. Ten specimens from each life stage (egg, larva, pupa, adult) were used for insecticidal activity assessment. Adulticidal activity was assessed using 10 cm2 filter paper strip, each treated with 0.200 mL of the test solution. Immature stages activities were evaluated using 23.76 cm2 discs of the same filter paper, each treated with 0.470 mL of the test solution. Mortality percentage was calculated using (number of dead insects × 100) / number of incubated insects. Probit analysis calculated LC50 values with a 95% confidence interval. RESULTS: Major EO constituents were ß-caryophyllene (EOCR), linalool (EOLH), linalyl acetate (EOSS), and limonene (EOCP). LC50 values were obtained for all stages except for the essential oil of C. paradisi. All oils showed repellent activity at 800 µg/cm2. OECR exhibited greater residual efficacy. CONCLUSION: Each EO demonstrated superior insecticidal activity against specific C. felis felis stages.

Asunto(s)

Ctenocephalides , Repelentes de Insectos , Insecticidas , Aceites Volátiles , Salvia , Animales , Aceites Volátiles/farmacología , Aceites Volátiles/química , Salvia/química , Repelentes de Insectos/farmacología , Repelentes de Insectos/química , Insecticidas/farmacología , Insecticidas/química , Ctenocephalides/efectos de los fármacos , Fabaceae/química , Lavandula/química , Larva/efectos de los fármacos , Pupa/efectos de los fármacos , Citrus/química , Monoterpenos Acíclicos/farmacología , Monoterpenos/farmacología , Monoterpenos/química , Aceites de Plantas/farmacología , Aceites de Plantas/química , Dosificación Letal Mediana

RESUMEN

In recent years, there has been a need for environmentally friendly compounds for weed management in agriculture. This study is aimed to assess the phytotoxic constituents of oils obtained from oleoresins of seven Copaifera species (known as copaiba oils). Copaiba oils were separated from the resins by hydro-distillation, and the distillates were analyzed using gas chromatography-mass spectrometry (GC-MS) to characterize their chemical compositions. Multivariate analyses and molecular networking of GC-MS data were conducted to discern patterns in the chemical composition and phytotoxic activity of the oils, with the aim of identifying key compounds associated with phytotoxic activity. Seed germination bioassay revealed strong or complete germination inhibition against the monocot, Agrostis stolonifera but not the dicot Lactuca sativa. GC-MS analysis showed variations in composition among Copaifera species with some common compounds identified across multiple species. Caryophyllene oxide and junenol were associated with the observed phytotoxic effects. Automated flash chromatography was used to isolate the major compounds of the oils. Isolated compounds exhibited differing levels of phytotoxicity compared to the oils, suggesting the importance of interactions or synergism among oil components. These findings highlight the potential of copaiba oils as natural herbicidal agents and underscore the importance of considering species-specific responses in weed management strategies.

Asunto(s)

Fabaceae , Cromatografía de Gases y Espectrometría de Masas , Germinación , Aceites Volátiles , Semillas , Fabaceae/química , Aceites Volátiles/química , Aceites Volátiles/toxicidad , Aceites Volátiles/farmacología , Semillas/química , Semillas/efectos de los fármacos , Semillas/crecimiento & desarrollo , Germinación/efectos de los fármacos , Aceites de Plantas/química , Aceites de Plantas/toxicidad , Aceites de Plantas/farmacología , Lactuca/efectos de los fármacos , Lactuca/crecimiento & desarrollo , Herbicidas/farmacología , Herbicidas/química , Herbicidas/toxicidad

RESUMEN

This study developed active packaging films of Polylactic acid incorporated with the plant-based essential oils of Trachyspermum ammi, T. ammi and Vanilla to package waffles, where the antimicrobial property was provided by T. ammi and its odor was masked by vanilla essential oil. Compared to conventional solvent-cast films of smaller sizes requiring a huge amount of solvents, bigger-size PLA-oil films with lower solvent demand were prepared by tape casting technique with 10, 30, and 50 wt% essential oil blends. Films were studied for their morphological, chemical, mechanical, barrier, and antimicrobial properties. The presence and time-bound release of volatile oils from the films was confirmed by infrared spectroscopy, with a continuous decrease of oils from the films till day 30. The plasticizing effect of oils in films was evidenced by decreased tensile strength and crystallinity. In contrast, an increase in elongation at break and water vapor permeability of oil films were also measured. Finally, when packed in PLA films containing 50 wt% blend of both oils, waffles shelf-life extended up to 30 days compared to 2 days for the neat PLA film, where Vanilla was found effective in masking the unpleasant odor of T.ammi as confirmed by sensory analysis.

Asunto(s)

Antiinfecciosos , Embalaje de Alimentos , Aceites Volátiles , Poliésteres , Poliésteres/química , Aceites Volátiles/química , Aceites Volátiles/farmacología , Embalaje de Alimentos/métodos , Antiinfecciosos/farmacología , Antiinfecciosos/química , Permeabilidad , Odorantes/análisis , Resistencia a la Tracción , Aceites de Plantas/química , Aceites de Plantas/farmacología

RESUMEN

The hepatoprotective effects of kiwifruit seed oil (KSO) were evaluated on acute liver injury (ALI) induced by carbon tetrachloride (CCl4) in vivo. Network pharmacology was used to predict active compounds and targets. Metabolomics and gut microbiota analyses were used to discover the activity mechanism of KSO. KSO improved the liver histological structure, significantly reduced serum proinflammatory cytokine levels, and increased liver antioxidant capacity. The metabolomics analysis showed that KSO may have hepatoprotective effects by controlling metabolites through its participation in signaling pathways like tryptophan metabolism, glycolysis/gluconeogenesis, galactose metabolism, and bile secretion. The gut microbiota analysis demonstrated that KSO improved the composition and quantity of the gut flora. Network pharmacological investigations demonstrated that KSO operated by altering Ptgs2, Nos2, Ppara, Pparg and Serpine1 mRNA levels. All evidence shows that KSO has a hepatoprotective effect, and the mechanism is connected to the regulation of metabolic disorders and intestinal flora.

Asunto(s)

Tetracloruro de Carbono , Enfermedad Hepática Inducida por Sustancias y Drogas , Microbioma Gastrointestinal , Hígado , Metabolómica , Aceites de Plantas , Semillas , Microbioma Gastrointestinal/efectos de los fármacos , Animales , Tetracloruro de Carbono/efectos adversos , Masculino , Aceites de Plantas/farmacología , Hígado/metabolismo , Hígado/efectos de los fármacos , Semillas/química , Ratones , Sustancias Protectoras/farmacología , Ratas