RESUMEN
The plant-specific WRINKLED1 (WRI1) is a member of the AP2/EREBP class of transcription factors that positively regulate oil biosynthesis in plant tissues. Limited information is available for the role of WRI1 in oil biosynthesis in castor bean (Ricinus connunis L.), an important industrial oil crop. Here, we report the identification of two alternatively spliced transcripts of RcWRI1, designated as RcWRI1-A and RcWRI1-B. The open reading frames of RcWRI1-A (1341 bp) and RcWRI1-B (1332 bp) differ by a stretch of 9 bp, such that the predicted RcWRI1-B lacks the three amino acid residues "VYL" that are present in RcWRI1-A. The RcWRI1-A transcript is present in flowers, leaves, pericarps and developing seeds, while the RcWRI1-B mRNA is only detectable in developing seeds. When the two isoforms were individually introduced into an Arabidopsiswri1-1 loss-of-function mutant, total fatty acid content was almost restored to the wild-type level, and the percentage of the wrinkled seeds was largely reduced in the transgenic lines relative to the wri1-1 mutant line. Transient expression of each RcWRI1 splice isoform in N. benthamiana leaves upregulated the expression of the WRI1 target genes, and consequently increased the oil content by 4.3-4.9 fold when compared with the controls, and RcWRI1-B appeared to be more active than RcWRI1-A. Both RcWRI1-A and RcWRI1-B can be used as a key transcriptional regulator to enhance fatty acid and oil biosynthesis in leafy biomass.
Asunto(s)
Aceite de Ricino/biosíntesis , Ácidos Grasos/biosíntesis , Nicotiana/genética , Proteínas de Plantas/genética , Factores de Transcripción/genética , Transgenes , Empalme Alternativo , Aceite de Ricino/genética , Ácidos Grasos/genética , Regulación de la Expresión Génica de las Plantas , Hojas de la Planta/metabolismo , Proteínas de Plantas/metabolismo , Ricinus/genética , Nicotiana/metabolismo , Factores de Transcripción/metabolismo , Regulación hacia ArribaRESUMEN
Bioenergy production combined with phytoremediation has been suggested to help in solving two critical world problems: the gradual reduction of fossil fuels and soil contamination. The aim of this research was to investigate the potential for the use of Ricinus communis L. (castor oil plant) as an energy crop and plant species to remediate metal-polluted sites. This study was performed in mine tailings containing high concentrations of Cu, Zn, Mn, Pb and Cd. Physico-chemical characterization, total, DTPA-extractable and water-soluble metals in rhizospheric tailings heap samples were carried. Metal concentrations in plant tissues and translocation factors (TFs) were also determined. The Ricinus seed-oil content was high between 41 and 64%, seeds from San Francisco site 6 had the highest oil content, while these from site 7 had the lowest. No trend between oil yield vs seed origin site was observed. Seed-oil content was negatively correlated with root concentration of Cu, Zn, Pb and Cd, but no correlation was observed with the extractable-metals. According to its shoot metal concentrations and TFs, castor bean is not a metal accumulator plant. This primary colonizing plant is well suited to cope with the local toxic conditions and can be useful for the stabilization of these residues, and for then decreasing metal bioavailability, dispersion and human health risks on these barren tailings heaps and in the surrounding area. Our work is the first report regarding combined oil production and a phytostabilization role for Ricinus plants in metal mine tailings and may give a new value to suitable metal-polluted areas.
Asunto(s)
Biodegradación Ambiental , Biocombustibles/análisis , Aceite de Ricino/biosíntesis , Ricinus communis/metabolismo , Ricinus communis/microbiología , Residuos Industriales/análisis , Metales Pesados/análisis , Minería , Micorrizas/fisiología , Contaminantes del Suelo/análisisRESUMEN
Acyl-acyl carrier protein (ACP) thioesterases are enzymes that terminate the intraplastidial fatty acid synthesis in plants by hydrolyzing the acyl-ACP intermediates and releasing free fatty acids to be incorporated into glycerolipids. These enzymes are classified in two families, FatA and FatB, which differ in amino acid sequence and substrate specificity. In the present work, both FatA and FatB thioesterases were cloned, sequenced and characterized from castor (Ricinus communis) seeds, a crop of high interest in oleochemistry. Single copies of FatA and FatB were found in castor resulting to be closely related with those of Jatropha curcas. The corresponding mature proteins were heterologously expressed in Escherichia coli for biochemical characterization after purification, resulting in high catalytic efficiency of RcFatA on oleoyl-ACP and palmitoleoyl-ACP and high efficiencies of RcFatB for oleoyl-ACP and palmitoyl-ACP. The expression profile of these genes displayed the highest levels in expanding tissues that typically are very active in lipid biosynthesis such as developing seed endosperm and young expanding leaves. The contribution of these two enzymes to the synthesis of castor oil is discussed.
Asunto(s)
Ricinus communis/enzimología , Tioléster Hidrolasas/metabolismo , Ricinus communis/metabolismo , Aceite de Ricino/análisis , Aceite de Ricino/biosíntesis , Datos de Secuencia Molecular , Estructura Molecular , Análisis de Secuencia de Proteína , Homología de Secuencia de Ácido NucleicoRESUMEN
SUMMARY: A central goal of green chemistry is to produce industrially useful fatty acids in oilseed crops. Although genes encoding suitable fatty acid-modifying enzymes are available from many wild species, progress has been limited because the expression of these genes in transgenic plants produces low yields of the desired products. For example, Ricinus communis fatty acid hydroxylase 12 (FAH12) produces a maximum of only 17% hydroxy fatty acids (HFAs) when expressed in Arabidopsis. cDNA clones encoding R. communis enzymes for additional steps in the seed oil biosynthetic pathway were identified. Expression of these cDNAs in FAH12 transgenic plants revealed that the R. communis type-2 acyl-coenzyme A:diacylglycerol acyltransferase (RcDGAT2) could increase HFAs from 17% to nearly 30%. Detailed comparisons of seed neutral lipids from the single- and double-transgenic lines indicated that RcDGAT2 substantially modified the triacylglycerol (TAG) pool, with significant increases in most of the major TAG species observed in native castor bean oil. These data suggest that RcDGAT2 prefers acyl-coenzyme A and diacylglycerol substrates containing HFAs, and biochemical analyses of RcDGAT2 expressed in yeast cells confirmed a strong preference for HFA-containing diacylglycerol substrates. Our results demonstrate that pathway engineering approaches can be used successfully to increase the yields of industrial feedstocks in plants, and that members of the DGAT2 gene family probably play a key role in this process.
Asunto(s)
Acilcoenzima A/metabolismo , Diacilglicerol O-Acetiltransferasa/genética , Ácidos Ricinoleicos/metabolismo , Ricinus communis/enzimología , Ricinus communis/genética , Secuencia de Aminoácidos , Arabidopsis/enzimología , Arabidopsis/genética , Aceite de Ricino/biosíntesis , ADN Complementario/genética , ADN de Plantas/genética , Biblioteca de Genes , Vectores Genéticos , Datos de Secuencia Molecular , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente/enzimología , Plantas Modificadas Genéticamente/genética , Saccharomyces cerevisiae/enzimología , Saccharomyces cerevisiae/genética , Semillas/enzimología , Semillas/genética , Análisis de Secuencia de Proteína , Homología de Secuencia de Aminoácido , Transformación Genética , Triglicéridos/biosíntesisRESUMEN
Polyamines with diamine structures of chain length longer than 3C were essential for the synthesis of phosphatidic acid (PA) from ricinoleoyl-CoA and lysophosphatidic acid (LPA) by the castor LPA acyltransferase reaction, suggesting that polyamines modulate enzyme affinity for the acyl-CoA substrate in vivo.
Asunto(s)
Aceite de Ricino/biosíntesis , Lisofosfolípidos/metabolismo , Poliaminas/farmacología , Acilcoenzima A/metabolismo , Acilación/efectos de los fármacos , Ácidos Fosfatidicos/biosíntesis , Ácidos Ricinoleicos/metabolismoRESUMEN
As part of a program to elucidate castor oil biosynthesis, we have identified 36 molecular species of PC and 35 molecular species of PE isolated from castor microsomes after incubations with [14C]-labeled FA. The six [14C]FA studied were ricinoleate, stearate, oleate, linoleate, linolenate, and palmitate, which were the only FA identified in castor microsomal incubations. The incorporation of each of the six FA into PC was better than that into PE. The [14C]FA were incorporated almost exclusively into the sn-2 position of both PC and PE. The incorporation of [14C]stearate and [14C]palmitate into 2-acyl-PC was slower compared to the other four [14C]FA. The incorporation does not show any selectivity for the various lysoPC molecular species. The level of incorporation of [14C]FA in PC was in the order of: oleate > linolenate > palmitate > linoleate > stearate > ricinoleate, and in PE: linoleate > linolenate > oleate > palmitate > stearate > ricinoleate. In general, at the sn-1 position of both PC and PE, linoleate was the most abundant FA, palmitate was the next, and oleate, linolenate, stearate, and ricinoleate were minor FA. The activities of oleoyl-12-hydroxylase, oleoyl-12-desaturase seem unaffected by the FA at the sn-1 position of 2-oleoyl-PC. The FA in the sn-1 position of PC does not significantly affect the activity of phospholipase A2, whereas ricinoleate is preferentially removed from the sn-2 position of PC. The results show that (i) [14C]oleate is most actively incorporated to form 2-oleoyl-PC, the immediate substrate of oleoyl-12-hydroxylase; (ii) 2-ricinoleoyl-PC is formed mostly by the hydroxylation of 2-oleoyl-PC, not from the incorporation of ricinoleate into 2-ricinoleoyl-PC; and (iii) 2-oleoyl-PE is less actively formed than 2-oleoyl-PC.
Asunto(s)
Aceite de Ricino/biosíntesis , Fosfatidilcolinas/análisis , Fosfatidiletanolaminas/análisis , Ricinus communis/química , Ricinus communis/metabolismo , Radioisótopos de Carbono , Ricinus communis/citología , Aceite de Ricino/química , Cromatografía Líquida de Alta Presión , Microsomas/química , Microsomas/metabolismo , Fosfatidilcolinas/aislamiento & purificación , Fosfatidiletanolaminas/aislamiento & purificación , EstereoisomerismoRESUMEN
We have examined the role of 2-oleoyl-PE (phosphatidylethanolamine) in the biosynthesis of triacylglycerols (TAG) by castor microsomes. In castor microsomal incubation, the label from 14C-oleate of 1-palmitoyl-2-[1-(14)C]oleoyl-sn-glycero-3-phosphoethanolamine is incorporated into TAG containing ricinoleate. The enzyme characteristics, such as optimal pH, and the effect of incubation components of the oleoyl-12-hydroxylase using 2-oleoyl-PE as incubation substrate are similar to those for 2-oleoyl-PC (phosphatidylcholine). However, compared to 2-oleoyl-PC, 2-oleoyl-PE is a less efficient incubation substrate of oleoyl-12-hydroxylase in castor microsomes. Unlike 2-oleoyl-PC, 2-oleoyl-PE is not hydroxylated to 2-ricinoleoyl-PE by oleoyl-12-hydroxylase and is not desaturated to 2-linoleoyl-PE by oleoyl-12-desaturase. We have demonstrated the conversion of 2-oleoyl-PE to 2-oleoyl-PC and vice versa. The incorporation of label from 2-[14C]oleoyl-PE into TAG occurs after its conversion to 2-oleoyl-PC, which can then be hydroxylated or desaturated. We detected neither PE-N-monomethyl nor PE-N,N-dimethyl, the intermediates from PE to PC by N-methylation. The conversion of 2-oleoyl-PE to 2-oleoyl-PC likely occurs via hydrolysis to 1,2-diacyl-sn-glycerol by phospholipase C and then by cholinephosphotransferase. This conversion does not appear to play a key role in driving ricinoleate into TAG.
Asunto(s)
Aceite de Ricino/biosíntesis , Microsomas/metabolismo , Ácidos Oléicos/metabolismo , Fosfatidilcolinas/metabolismo , Fosfatidilcolinas/fisiología , Fosfatidiletanolaminas/metabolismo , Fosfatidiletanolaminas/fisiología , Cromatografía Líquida de Alta Presión , Escherichia coli/metabolismo , Ácidos Grasos/metabolismo , Concentración de Iones de Hidrógeno , Hidroxilación , Ácidos Oléicos/fisiología , Proteínas Recombinantes/química , Ácidos Ricinoleicos/química , Streptomyces antibioticus/enzimología , Factores de TiempoRESUMEN
Castor oil is 90% ricinoleate (12-hydroxy-oleate) and has numerous industrial uses. Components of castor bean (Ricinus communis L.) pose serious problems to processors. We are evaluating two complementary approaches to providing a safe source of castor oil.