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1.
Materials (Basel) ; 17(12)2024 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-38930314

RESUMEN

Polypropylene fiber reinforcement is an effective method to enhance the durability of concrete structures. With the increasing public interest in the widespread use of polypropylene fiber reinforced concrete (PFRC), the necessity of evaluating the mechanism of polypropylene fiber (PF) on the permeability of concrete has become prominent. This paper describes the influence of PF on the concrete permeability exposed to freeze-thaw cycles under compressive and tensile stress. The permeability of PFRC under compressive and tensile loads is accurately measured by a specialized permeability setup. The permeability of PFRC under compressive and tensile loads, the volume change of PFRC under compressive load, and the relationship between compressive stress levels at minimum permeability and minimum volume points of PFRC are discussed. The results indicate that the addition of PF adversely affects the permeability of concrete without freeze-thaw damage and cracks. However, it decreases the permeability of concrete specimens exposed to freeze-thaw cycles and cracking. Under compressive load, the permeability of PFRC initially decreases slowly and follows by a significant increase as the compressive stress level increases. This phenomenon correlates with the volume change of the specimen. The compressive stress level of the minimum permeability point and compressive stress level of the minimum volume point of PFRC exhibit a linear correlation, with a fitted proportional function parameter γ ≈ 0.98872. Under tensile load, the permeability of PFRC increases gradually with radial deformation and follows by a significant increase. The strain-permeability curves of PFRC under loading are studied and consist of two stages. In stage I, the permeability of PFRC gradually decreases with the increase of strain under compressive load, while the permeability increases with the increase of strain under tensile load. In stage II, under compressive load, the permeability of PFRC increases with the increase of freeze-thaw cycles, whereas under tensile load, the permeability gradually decreases with the increase of freeze-thaw cycles. The reduction of PF on the permeability of PFRC under tensile load is greater than that under compressive load. In future research, the relationship between strain and permeability of PFRC can be integrated with its constitutive relationship between stress and strain to provide a reference for the application of PF in the waterproofing of concrete structures.

2.
Materials (Basel) ; 17(9)2024 Apr 30.
Artículo en Inglés | MEDLINE | ID: mdl-38730922

RESUMEN

Hybrid bonded-bolted composite material interference connections significantly enhance the collaborative load-bearing capabilities of the adhesive layer and bolts, thus improving structural load-carrying capacity and fatigue life. So, these connections offer significant developmental potential and application prospects in aircraft structural assembly. However, interference causes damage to the adhesive layer and composite laminate around the holes, leading to issues with interface damage. In this study, we employed experimental and finite element methods. Initially, different interference-fit sizes were selected for bolt insertion to analyze the damage mechanism of the adhesive layer during interference-fit bolt installation. Subsequently, a finite element tensile model considering damage to the adhesive layer and composite laminate around the holes post-insertion was established. This study aimed to investigate damage in composite bonded-bolted hybrid joints, explore load-carrying rules and failure modes, and reveal the mechanisms of interference effects on structural damage and failure. The research results indicate that the finite element prediction model considering initial damage around the holes is more effective. As the interference-fit size increases, damage to the adhesive layer transitions from surface debonding to local cracking, while damage to the composite matrix shifts from slight compression failure to severe delamination and fiber-bending fracturing. The structural strength shows a trend of initially increasing and then decreasing, with the maximum strength observed at an interference-fit size of 1.1%.

3.
Materials (Basel) ; 17(8)2024 Apr 10.
Artículo en Inglés | MEDLINE | ID: mdl-38673091

RESUMEN

This paper describes the effects of macro fibers on permeability and crack surface topography of layered fiber-reinforced concrete (FRC) specimens with different layering ratios under uniaxial tensile load. The crack permeability of layered FRC specimens is investigated by a self-designed permeability setup. The topographical analysis of crack surfaces is investigated by a custom-designed laser scanning setup. The results show that when the fiber volume content and layering ratio of the FRC layer are constant, the tensile toughness of layered FRC specimens depends on the proportion of steel fiber in macro fibers, and with an increase in the proportion of steel fiber, the tensile toughness of layered FRC specimens increases. For the layered FRC specimens, the crack permeability is much lower than that of the normal concrete (NC) specimen. A significant positive synergistic effect on crack impermeability can be achieved by the combination of steel fiber and polypropylene fiber in the SF80PP2.3 specimen. The crack surface roughness parameter (Rn) values of the NC layer in layered FRC specimens are all higher than those of the NC specimen, and the crack surface Rn of the FRC layer in layered FRC specimens is higher than that of the unlayered FRC specimens. This can effectively increase the head loss of cracks and reduce the crack permeability of layered FRC specimens.

4.
Materials (Basel) ; 17(8)2024 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-38673176

RESUMEN

This paper describes hybrid fiber's influence on the crack permeability of cracked concrete exposed to freeze-thaw cycles. A permeability setup and a laser-scanning setup have been designed to measure the crack permeability and the fractured surface roughness of cracked hybrid fiber-reinforced concrete, containing polypropylene fiber and steel fiber, under a splitting tensile load. The results show that, when the effective crack width of the specimens is less than 25 µm, the rough crack surface significantly reduces the concrete's crack permeability. As the crack width increases, the effect of the concrete crack surface on crack permeability gradually decreases, and the crack permeability of the concrete is closer to the Poiseuille flow model. The permeability parameter α derived from the Poiseuille flow model is effective for assessing the crack permeability of concrete. Compared to the modified factor ξ of crack permeability, the permeability parameter α can effectively evaluate and quantify the development trend of crack permeability within a certain range of crack widths. The permeability parameter α of SF20PP2.3, subjected to the same freeze-thaw cycles, decreases by 16.3-94.8% compared to PP4.6 and SF40, and SF20PP2.3 demonstrates a positive synergistic effect on the crack impermeability of cracked concrete. The crack impermeability of SF40PP2.3, subjected to the same freeze-thaw cycles, lies between that of PP6.9 and SF60. The roughness of crack surface (X) and the crack permeability (Y) are highly correlated and follow an exponential curve (Y = 1.0415 × 107·e-6.025·X) in concrete. This demonstrates that hybrid fibers enhance crack impermeability by increasing the crack surface roughness. Furthermore, the combination of polypropylene fiber and steel fiber effectively promotes the formation of micro-cracks and facilitates the propagation of multiple cracks in the concrete matrix. This combination increases the head loss of water flow through the concrete and decreases the crack permeability.

5.
BMC Oral Health ; 23(1): 186, 2023 03 30.
Artículo en Inglés | MEDLINE | ID: mdl-36997908

RESUMEN

BACKGROUND: In implant prosthetic dentistry, the adhesive connection of individualized ceramic crowns and prefabricated titanium bases leads to several benefits. However, the durability of the bonding could be a weak point and especially depends on sufficient surface pretreatment. Cold atmospheric-pressure plasma (CAP) is a pretreatment method that should improve the surface properties without physical damage. Thus, the purpose of this study was to investigate the influence of CAP treatment on pull-off tensile load in two-piece abutment crowns. METHODS: Eighty zirconia crowns and titanium bases were divided into eight groups (n = 10) according to their surface pretreatment prior to cementation with Panavia V5: no treatment (A); sandblasting (B); 10-MDP primer (C); sandblasting and primer (D); CAP (AP); sandblasting and CAP (BP); CAP and primer (CP); sandblasting, CAP and primer (DP). The specimens were thermocycled (5°/55°, 5000 cycles), and then the pull-off tensile load (TL) was measured. Statistical analyses were performed using three-way ANOVA with Tukey post-hoc and Fisher's exact tests. RESULTS: The results showed that the TL was highest in group D (p < 0.0001). Some combinations of different treatments led to effects that were greater than the sum of the individual effects. These effects were modified by interactions. Only in combination with primer, CAP treatment had a small but positive significant effect (group CP vs. C and CP vs. AP, p < 0.0001) which however did not come close to the strong interaction effect that resulted from the combination of sandblasting and primer. CONCLUSION: Within the limitations of this study, CAP treatment cannot be recommended in this specific field of indication due to its unreliable influence on TL in combination with other pretreatment methods.


Asunto(s)
Coronas , Titanio , Humanos , Cementación , Ensayo de Materiales , Análisis del Estrés Dental , Propiedades de Superficie , Cementos de Resina
6.
Appl Biochem Biotechnol ; 195(6): 3981-3993, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-35451793

RESUMEN

The influence of temperature, pollutant, and pH on the local corrosion rate of insulators installed in industrial, marine, and rural installation sites is investigated based on experimental and statistical investigations. The tensile load test confirms that corroded insulator specimens collected from industrial sites aged more than 10 years represent a minimum fracture load, 19,892 lbs. It was further observed that more than 91.24% and 64.62% corroded insulator specimens suffered from shell break and pin detachment, respectively. The microstructural and XRF analysis reveal that insulator specimens collected from industrial sites (age > 10 years), represented the highest wt% of O (19.2) and lowest wt% of Zn (0.34) among industrial, marine, and rural installation sites. The 3D stationery mechanical simulation reveals that insulator specimens aged > 10 years experienced maximum stress (600 MPa) in the pin-cement interface. Using full two-level factorial designs, temperature, concentration of pollutants, and pH were found significant factors for corrosion rate. The immersion test results further confirm the above-mentioned factors significant for the dissolution behavior of galvanized coating of insulator pin. Following immersion test results, the industrial region shows the highest corrosion rate (5.58-12 µm/year) among all installation sites.


Asunto(s)
Aleaciones , Ensayo de Materiales , Aleaciones/química , Corrosión , Temperatura
7.
Materials (Basel) ; 14(19)2021 Oct 06.
Artículo en Inglés | MEDLINE | ID: mdl-34640229

RESUMEN

In recent years, many investigations on the development of innovative dressing materials with potential applications, e.g., for cytostatics delivery, have been performed. One of the most promising carriers is albumin, which tends to accumulate near cancer cells. Here, chitosan-based hydrogels containing albumin spheres and Aloe vera juice, designed for the treatment of skin cancers or burn wounds resulting from radiotherapy, were developed. The presence of albumin in hydrogel matrices was confirmed via Fourier transform infrared (FT-IR) and Raman spectroscopy. Albumin spheres were clearly visible in microscopic images. It was proved that the introduction of albumin into hydrogels resulted in their increased resistance to the tensile load, i.e., approximately 30% more force was needed to break such materials. Modified hydrogels showed approximately 10% more swelling ability. All hydrogels were characterized by hydrophilicity (contact angles were <90°) which may support the regeneration of epithelial cells and non-cytotoxicity towards murine fibroblasts L929 and released Aloe vera juice more effectively in an acidic environment than in a neutral one wherein spheres introduced into the hydrogel matrix extended the release time. Thus, the developed materials, due to their chemical composition and physicochemical properties, constitute promising materials with great application potential for biomedical purposes.

8.
Materials (Basel) ; 14(11)2021 May 25.
Artículo en Inglés | MEDLINE | ID: mdl-34070350

RESUMEN

The aim of this study is to obtain the stress intensity factor (SIF) along the crack front of elliptical cracks located in finite-thickness plates subjected to imposed displacement or applied tensile load, for different crack geometries (relative depths and aspect ratios) and crack configurations (embedded, surface, and corner). The SIF was calculated from the J-integral, obtained by the finite element method. The results show how the SIF grows with the increase in the relative crack depth and with the decrease in the aspect ratio, with the corner crack being the most dangerous configuration and the embedded crack the most favorable configuration. By increasing the plate length, the SIF rises when the plate is under imposed displacement and decreases when the plate is subjected to applied tensile load, both cases tending towards the same SIF curve.

9.
Carbohydr Polym ; 230: 115615, 2020 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-31887906

RESUMEN

Mechano-sorptive creep, i.e. the increased rate of creep that occurs during changing relative humidity, when loading paper or wood, is a phenomenon still not fully understood. This phenomenon was here investigated by examining the changes occurring at the molecular level utilising FTIR spectroscopy. By subjecting the paper to deuterated water, the changes involving both the crystalline hydroxyls as well as those in accessible regions could be examined. During loading, the cellulose molecular chains are stretched taking the load. In addition, during mechano-sorptive creep a further exchange from OH to OD groups occurred. This was interpreted as caused by slippage between cellulose fibrils allowing previously non-accessible hydroxyls to become available for deuterium exchange. Thus, the loosening of the structure, during the changing moisture conditions, is interpreted as what has led to the increased creep and the possibility for new areas of cellulose fibril/fibril aggregates to be exposed to the deuterium exchange.


Asunto(s)
Celulosa/química , Celulosa/normas , Humedad , Espectroscopía Infrarroja por Transformada de Fourier , Resistencia a la Tracción , Soporte de Peso , Humectabilidad
10.
Polymers (Basel) ; 11(6)2019 Jun 12.
Artículo en Inglés | MEDLINE | ID: mdl-31212820

RESUMEN

This paper reports the auxetic behavior of modified conventional non-woven fabric. The auxetic behavior of fabric was achieved by forming rotating square unit geometry with a highly ordered pattern of slits by laser cutting. Two commercial needle-punched non-woven fabric used as lining and the reinforcement fabric for the footwear industry were investigated. The influence of two rotating square unit sizes was analyzed for each fabric. The original and modified fabric samples were subjected to quasi-static tensile load by using the Tinius Olsen testing machine to observe the in-plane mechanical properties and deformation behavior of tested samples. The tests were recorded with a full high-definition (HD) digital camera and the video recognition technique was applied to determine the Poisson's ratio evolution during testing. The results show that the modified samples exhibit a much lower breaking force due to induced slits, which in turn limits the application of such modified fabric to low tensile loads. The samples with smaller rotating cell sizes exhibit the highest negative Poisson's ratio during tensile loading through the entire longitudinal strain range until rupture. Non-woven fabric with equal distribution and orientation of fibers in both directions offer better auxetic response with a smaller out-of-plane rotation of rotating unit cells. The out-of-plane rotation of unit cells in non-homogenous samples is higher in machine direction.

11.
J Hazard Mater ; 366: 65-77, 2019 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-30502574

RESUMEN

Highly pressurized injection pipelines in CO2 flooding often suffer from different types of corrosion defects and mechanical stresses and can easily cause serious damage to the environment. Currently, the descending rule of pipe burst pressures under different defect parameters and tensile loads has not been studied systematically. In this study, a section of injection pipe was used to simulate the burst process of pipe with groove and general corrosion defects. The crack appearance showed that the fracture began at the centre of the longitudinal defect line as an instantaneous ductile rupture and then extended along both sides as a rapid brittle rupture under high stress. The burst pressure in the groove corrosion defect tests showed that the defect depth played a more dominant role in pipe burst than the defect length did, and when a 30 MPa axial tensile load was added, the pipe burst pressure was reduced approximately 20-30%. In general corrosion defect tests, the burst pressures were generally much lower than the groove corrosion defect tests produced, and when adding the axial tensile load, the burst mode changed. Moreover, the value of the burst pressure can be a sign of BLEVE (boiling liquid expanding vapor explosion) severity.

12.
MethodsX ; 5: 924-932, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30167382

RESUMEN

Mechanical loading is an important cue for directing stem cell fate and engineered tissue formation in vitro. Stem cells cultured on 2-dimensional (D) substrates and in 3D scaffolds have been shown to differentiate toward bone, tendon, cartilage, ligament, and skeletal muscle lineages depending on their exposure to mechanical stimuli. To apply this mechanical stimulus in vitro, mechanical bioreactors are needed. However, current bioreactor systems are challenged by their high cost, limited ability for customization, and lack of force measurement capabilities. We demonstrate the use of 3-dimensional printing (3DP) technology to design and fabricate a low-cost custom bioreactor system that can be used to apply controlled mechanical stimuli to cells in culture and measure the mechanical properties of small soft tissues. The results of our in vitro studies and mechanical evaluations show that 3DP technology is feasible as a platform for developing a low-cost, customizable, and multifunctional mechanical bioreactor system. • 3DP technology was used to print a multifunctional bioreactor system/tensile load frame for a fraction of the cost of commercial systems. • The system mechanically stimulated cells in culture and evaluated the mechanical properties of soft tissues. • This system is easily customizable and can be used to evaluate multiple types of soft tissues.

13.
Scand J Med Sci Sports ; 27(12): 1681-1687, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-28207972

RESUMEN

In this study, detailed investigations into the shape of the inferior patellar pole, the site of the patellar tendon attachment, and the length and course of the patellar tendon were performed with the aim of examining the anatomical factors involved in the developmental mechanism of patellar tendinitis. The investigation examined 100 legs from 50 cadavers. The inferior patellar pole was classified into three types: pointed, intermediate, and blunt. The attachment of the patellar tendon to the inferior patellar pole was classified into two types: an anterior and a posterior. The length of the patellar tendon was measured from the tibial tuberosity to the inferior patellar pole. The pointed type was seen in 57% of legs, the intermediate type in 21%, and the blunt type in 22%. Twenty-one legs were the pointed type, as well as the anterior type. The patellar tendon was significantly shorter with the posterior type than with the anterior type. The blunt type also had a significantly shorter patellar tendon than the pointed type. In legs that were both the pointed type and the anterior type, the inferior patellar pole and the proximal posterior surface of the patellar tendon impinged during knee flexion due to the posterior tilt of the patella, suggesting the possibility that this may induce damage. With the posterior type and blunt type, on the other hand, the possibility of strong tensile stress on the tendon fibers of the posterior facet of the inferior patellar pole was suggested.


Asunto(s)
Rótula/anatomía & histología , Ligamento Rotuliano/anatomía & histología , Anciano , Anciano de 80 o más Años , Pueblo Asiatico , Cadáver , Disección , Femenino , Humanos , Japón , Masculino , Resistencia a la Tracción , Tibia/anatomía & histología
14.
Ann Bot ; 118(4): 747-762, 2016 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-27456136

RESUMEN

Background and Aims Storms can cause huge damage to European forests. Even pole-stage trees with 80-cm rooting depth can topple. Therefore, good anchorage is needed for trees to survive and grow up from an early age. We hypothesized that root architecture is a predominant factor determining anchorage failure caused by strong winds. Methods We sampled 48 seeded or planted Pinus pinaster trees of similar aerial size from four stands damaged by a major storm 3 years before. The trees were gathered into three classes: undamaged, leaning and heavily toppled. After uprooting and 3D digitizing of their full root architectures, we computed the mechanical characteristics of the main components of the root system from our morphological measurements. Key Results Variability in root architecture was quite large. A large main taproot, either short and thick or long and thin, and guyed by a large volume of deep roots, was the major component that prevented stem leaning. Greater shallow root flexural stiffness mainly at the end of the zone of rapid taper on the windward side also prevented leaning. Toppling in less than 90-cm-deep soil was avoided in trees with a stocky taproots or with a very big leeward shallow root. Toppled trees also had a lower relative root biomass - stump excluded - than straight trees. Conclusions It was mainly the flexural stiffness of the central part of the root system that secured anchorage, preventing a weak displacement of the stump. The distal part of the longest taproot and attached deep roots may be the only parts of the root system contributing to anchorage through their maximum tensile load. Several designs provided good anchorage, depending partly on available soil depth. Pole-stage trees are in-between the juvenile phase when they fail by toppling and the mature phase when they fail by uprooting.

15.
J Orthop Res ; 34(5): 852-9, 2016 05.
Artículo en Inglés | MEDLINE | ID: mdl-26509464

RESUMEN

The optimal mechanical loading regimen for the healing of a tendon graft in a bone tunnel is unknown. We developed a rat model that directly tensions a healing tendon graft, without the use of confounding joint motion. Fifty cycles of either 0, 3, or 6 N of tension were applied to groups daily for 3 or 6 weeks. At 3 weeks the low load (3 N) group had the highest failure load (p = 0.009), but by 6 weeks there were no differences in failure load among groups. At 3 weeks the high load (6 N) group had greater osteoclast activity compared to the immobilized (0 N) group (p < 0.05), and by 6 weeks there were significantly more osteoclasts in the high load group compared to the low load group (p = 0.01). Bone volume fraction was higher in the immobilized group compared to the 3 N load group at 3 weeks (p = 0.014) and 6 weeks (p = 0.007). At 6 weeks, the immobilized group had greater trabecular number compared to both loading groups (p < 0.05). In conclusion, low magnitude loading had a beneficial early effect but continued loading led to poorer new bone formation over time and no beneficial effect at 6 weeks, perhaps due to delayed maturation from cumulative loads. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:852-859, 2016.


Asunto(s)
Plastía con Hueso-Tendón Rotuliano-Hueso , Inmovilización , Modelos Animales , Regeneración , Tendones/fisiología , Animales , Masculino , Distribución Aleatoria , Ratas Sprague-Dawley , Tendones/patología , Soporte de Peso , Microtomografía por Rayos X
16.
J Vet Sci ; 14(2): 175-84, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23628657

RESUMEN

We evaluated the biological scaffold properties of canine small intestinal submucosa (SIS) compared to a those of polypropylene mesh in growing rats with full-thickness abdominal defects. SIS is used to repair musculoskeletal tissue while promoting cell migration and supporting tissue regeneration. Polypropylene mesh is a non-resorbable synthetic material that can endure mechanical tension. Canine SIS was obtained from donor German shepherds, and its porous collagen fiber structure was identified using scanning electron microscopy (SEM). A 2.50-cm(2) section of canine SIS (SIS group) or mesh (mesh group) was implanted in Sprague-Dawley rats. At 1, 2, 4, 12, and 24 weeks after surgery, the implants were histopathologically examined and tensile load was tested. One month after surgery, CD68+ macrophage numbers in the SIS group were increased, but the number of CD8+ T cells in this group declined more rapidly than that in rats treated with the mesh. In the SIS group, few adhesions and well-developed autologous abdominal muscle infiltration into the SIS collagen fibers were observed. No significant differences in the tensile load test results were found between the SIS and mesh groups at 24 weeks. Canine SIS may therefore be a suitable replacement for artificial biological scaffolds in small animals.


Asunto(s)
Pared Abdominal/cirugía , Materiales Biocompatibles/uso terapéutico , Intestino Delgado/trasplante , Polipropilenos/uso terapéutico , Andamios del Tejido , Trasplante Heterólogo/métodos , Cicatrización de Heridas , Animales , Perros , Femenino , Mucosa Intestinal/citología , Mucosa Intestinal/trasplante , Intestino Delgado/citología , Ratas , Ratas Sprague-Dawley , Resistencia a la Tracción , Adherencias Tisulares
17.
Knee ; 20(6): 577-80, 2013 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23340094

RESUMEN

PURPOSE: Comparison of the mechanical characteristics of meniscal repair fixation using horizontal sutures and six different sutures under submaximal cyclic and load to failure test conditions may aid physicians in selecting a suture type. METHODS: A 2-cm long anteroposterior vertical longitudinal incision was created in six groups of bovine medial menisci. Lesions were repaired using a No. 2 suture either composed of polyester or polyester and ultra high-molecular weight polyethylene (UHMWPE), or UHMWPE and polydioxanone or pure UHMWPE. Endpoints included ultimate failure load (N), pull-out stiffness (N/mm), pull-out displacement (mm), cyclic displacement (mm) after 100cycles, after 500cycles, and mode of failure. RESULTS: Polyester suture had lower ultimate load than all groups except the suture composed of polyester and UHMWPE (P<.05). Pure UHMWPE suture had higher ultimate failure load than sutures composed of either polyester or polyester plus UHMWPE (P<.05). Predominant failure mode was suture cutting through the meniscus for the groups except for polyester suture which failed by suture rupture. CONCLUSION: Under cyclic loading conditions in bovine meniscus, braided polyester suture fixation provided lower initial fixation strength than fixation with various high strength sutures composed of pure UHMWPE or a combination of absorbable monofilament polydioxanone and UHMWPE, except for combination of polyester and UHMWPE sutures. CLINICAL RELEVANCE: Present study does not support the usage of the braided polyester sutures instead of high strength sutures composed either partially or totally of ultra-high molecular weight polyethylene for the horizontal suture configuration of meniscus repair.


Asunto(s)
Meniscos Tibiales/cirugía , Anclas para Sutura , Técnicas de Sutura , Suturas , Resistencia a la Tracción , Análisis de Varianza , Animales , Fenómenos Biomecánicos , Bovinos , Cápsula Articular/cirugía , Articulación de la Rodilla/cirugía , Ensayo de Materiales , Modelos Animales , Poliésteres/farmacología , Polietilenos/farmacología , Estrés Mecánico , Recolección de Tejidos y Órganos
18.
Int J Organ Transplant Med ; 4(3): 96-101, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-25013660

RESUMEN

BACKGROUND: Human skin allograft has been used as wound coverage for a long time; it is one of the most successful and widely used dressings for burn wounds in the world. OBJECTIVE: To prepare a freeze-dried human split-thickness skin allograft and evaluate its cytotoxicity, the structure and physical properties after processing methods and clinical efficacy in burn patients. METHODS: After ensuring tissue safety, we lyophilized human cadaveric partial thickness skin and exposed it to gamma radiation. Histopathological and immunohistochemical properties, tensile strength and in vitro cytotoxicity were assayed for the skin samples. Then, we tested the samples in 11 patients with deep skin burn. RESULTS: On histological and histopathological examinations, we found a normal skin structure. The tensile strength of the rehydrated freeze-dried human skin allograft was not lesser than the fresh human skin. Cell viability in MTT testing was more than 95%. None of our patients showed any signs of immunological reactions or complications. CONCLUSION: Gamma-irradiated freeze-dried human split-thickness skin is safe and non-toxic and can be used for the treatment of patients with deep skin burn.

19.
Artículo en Inglés | WPRIM (Pacífico Occidental) | ID: wpr-104701

RESUMEN

We evaluated the biological scaffold properties of canine small intestinal submucosa (SIS) compared to a those of polypropylene mesh in growing rats with full-thickness abdominal defects. SIS is used to repair musculoskeletal tissue while promoting cell migration and supporting tissue regeneration. Polypropylene mesh is a non-resorbable synthetic material that can endure mechanical tension. Canine SIS was obtained from donor German shepherds, and its porous collagen fiber structure was identified using scanning electron microscopy (SEM). A 2.50-cm2 section of canine SIS (SIS group) or mesh (mesh group) was implanted in Sprague-Dawley rats. At 1, 2, 4, 12, and 24 weeks after surgery, the implants were histopathologically examined and tensile load was tested. One month after surgery, CD68+ macrophage numbers in the SIS group were increased, but the number of CD8+ T cells in this group declined more rapidly than that in rats treated with the mesh. In the SIS group, few adhesions and well-developed autologous abdominal muscle infiltration into the SIS collagen fibers were observed. No significant differences in the tensile load test results were found between the SIS and mesh groups at 24 weeks. Canine SIS may therefore be a suitable replacement for artificial biological scaffolds in small animals.


Asunto(s)
Animales , Perros , Femenino , Ratas , Pared Abdominal/cirugía , Materiales Biocompatibles/uso terapéutico , Mucosa Intestinal/citología , Intestino Delgado/citología , Polipropilenos/uso terapéutico , Ratas Sprague-Dawley , Resistencia a la Tracción , Adherencias Tisulares , Andamios del Tejido , Trasplante Heterólogo/métodos , Cicatrización de Heridas
20.
Artículo en Coreano | WPRIM (Pacífico Occidental) | ID: wpr-32841

RESUMEN

OBJECTIVES: The purpose of this study is to compare ultimate tensile load of newly designed loop suture technique, to those of Pulvertaft fishmouth suture technique and Kessler suture technique with core strands. MATERIALS AND METHODS: Eight week-old Habbard chickens were sacrificed to harvest flexor digitorum logus tendon of long toe. They were divided into four groups according to suture technique; interweave suture group, loop suture group, Kessler suture group, and normal control group. Twenty tendons were tested in each group. Comparison of cross-sectional areas between each technique was verified by statistical method and the difference was not statistically significant (p>0.05). Tensile load and deformed length were checked by Instron (Model 1000, Instron Corp, Canton, MA). ANOVA test was used for statistical analysis. RESULTS: Ultimate tensile loads were 22.83+/-7.89 N in interweave suture group, 30.58+/-5.96 N in loop suture group, and 10.83+/-4.47 N in Kessler suture group. These results showed statistically significant differences (p<0.001). The values were 33 % in interweave suture, 44% in loop suture, and 15 % in Kessler's suture respectively. Absorbed energy were 0.48+/-0.32 J in interweave suture group, 0.61+/-0.18 J in loop suture group, and 0.22+/-0.15 J in Kessler suture group, and 1.01+/-0.20 J in normal control group. There were statisti - cally significant differences between each groups (p<0.01). CONCLUSION: The loop suture technique showed better biomechanical properties than interweave or Kessler technique. We think the loop suture technique is a simple and useful method, especially for tendon transfer or tendon graft when tendon length is sufficiently long to make a good tendon overlap.


Asunto(s)
Pollos , Técnicas de Sutura , Suturas , Transferencia Tendinosa , Tendones , Dedos del Pie , Trasplantes
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