RESUMEN

We present in this paper a framework for damage detection and localization using neural networks. The data we use to train the network are m×d pixel images consisting of measurements of the relative variations of m natural frequencies of the structure under monitoring over a period of d-days. To measure the relative variations of the natural frequencies, we use the stretching method, which allows us to obtain reliable measurements amidst fluctuations induced by environmental factors such as temperature variations. We show that even by monitoring a single natural frequency over a few days, accurate damage detection can be achieved. The accuracy for damage detection significantly improves when a small number of natural frequencies is monitored instead of a single one. More importantly, monitoring multiple natural frequencies allows for damage localization provided that the network can be trained for both healthy and damaged scenarios. This is feasible under the assumption that damage occurs at a finite number of damage-prone locations. Several results obtained with numerically simulated data illustrate the effectiveness of the proposed approach.

Asunto(s)

Redes Neurales de la Computación

RESUMEN

The objective of the present study was to prepare and evaluate artemether-loaded poly (lactic-co-glycolic acid) (PLGA) nanorods by mechanical stretching of nanospheres. Artemether-loaded PLGA nanospheres were prepared by the standard nanoprecipitation method. To prepare the nanorods, nanospheres (129 nm) were embedded in polyvinyl alcohol film. The film was stretched by using an in-house fabricated film stretching apparatus in one dimension at the rate of 10 mm/min in acetone or silicon oil. Nanorods were recovered by dissolving the film in Milli-Q-water after stretching. The effect of film thickness (100 µm vs 150 µm), the ratio of lactide to glycolide in PLGA (50:50 vs 75:25), extent of stretching (2x vs 4x), on the aspect ratio of the nanorods was studied. A sustained release of artemether was observed from both nanospheres and nanorods with almost 85% drug release at the end of 72 h. In cytotoxicity study, almost 90% cell viability was found when THP-1 cells were treated with artemether, nanospheres, and nanorods equivalent to 0.001 to 100 µg/mL of artemether. At all the concentrations of artemether, nanorods showed less haemolysis of RBCs than the nanospheres. Artemether-loaded PLGA nanorods could be successfully prepared by the film stretching method for intravenous delivery of antimalarial drugs.

Asunto(s)

Nanosferas , Nanotubos , Arteméter , Portadores de Fármacos , Ácido Láctico , Tamaño de la Partícula , Ácido Poliglicólico , Copolímero de Ácido Poliláctico-Ácido Poliglicólico

RESUMEN

We present in this paper a structural health monitoring study of the Egyptian lighthouse of Rethymnon in Crete, Greece. Using structural vibration data collected on a limited number of sensors during a 3-month period, we illustrate the potential of the stretching method for monitoring variations in the natural frequencies of the structure. The stretching method compares two signals, the current that refers to the actual state of the structure, with the reference one that characterizes the structure at a reference healthy condition. For the structure under study, an 8-day time interval is used for the reference quantity while the current quantity is computed using a time window of 24 h. Our results indicate that frequency shifts of 1% can be detected with high accuracy allowing for early damage assessment. We also provide a simple numerical model that is calibrated to match the natural frequencies estimated using the stretching method. The model is used to produce possible damage scenarios that correspond to 1% shift in the first natural frequencies. Although simple in nature, this model seems to deliver a realistic response of the structure. This is shown by comparing the response at the top of the structure to the actual measurement during a small earthquake. This is a preliminary study indicating the potential of the stretching method for structural health monitoring of historical monuments. The results are very promising. Further analysis is necessary requiring the deployment of the instrumentation (possibly with additional instruments) for a longer period of time.

RESUMEN

Particle shape has been recognized as one of the key properties of nanoparticles in biomedical applications including targeted drug delivery. Targeting ability of shape-engineered particles depends largely on targeting ligands conjugated on the particle surface. However, poor capacity for surface ligand conjugation remains a problem in anisotropic nanoparticles made with biodegradable polymers such as PLGA. In this study, we prepared anisotropic PLGA nanoparticles with abundant conjugatable surface functional groups by a film stretching-based fabrication method with poly (ethylene-alt-maleic acid) (PEMA). Scanning electron microscopy images showed that microrods and nanorods were successfully fabricated by the PEMA-based film stretching method. The presence of surface carboxylic acid groups was confirmed by confocal microscopy and zeta potential measurements. Using the improved film-stretching method, the amount of protein conjugated to the surface of nanorods was increased three-fold. Transferrin-conjugated, nanorods fabricated by the improved method exhibited higher binding and internalization than unmodified counterparts. Therefore, the PEMA-based film-stretching system presented in this study would be a promising fabrication method for non-spherical biodegradable polymeric micro- and nanoparticles with high capacity of surface modifications for enhanced targeted delivery.