RESUMEN

Background: Breast cancer presents significant challenges due to the limited effectiveness of available treatments and the high likelihood of recurrence. iRGD possesses both RGD sequence and C-terminal sequence and has dual functions of targeting and membrane penetration. iRGD-modified nanocarriers can enhance drug targeting of tumor vascular endothelial cells and penetration of new microvessels, increasing drug concentration in tumor tissues. Methods: The amidation reaction was carried out between SiO2/AuNCs and iRGD/PTX, yielding a conjugated drug delivery system (SiO2/AuNCs-iRGD/PTX, SAIP@NPs). The assessment encompassed the characterization of the morphology, particle size distribution, physicochemical properties, in vitro release profile, cytotoxicity, and cellular uptake of SAIP@NPs. The tumor targeting and anti-tumor efficacy of SAIP@NPs were assessed using a small animal in vivo imaging system and a tumor-bearing nude mice model, respectively. The tumor targeting and anti-tumor efficacy of SAIP@NPs were assessed utilizing a small animal in vivo imaging system and an in situ nude mice breast cancer xenograft model, respectively. Results: The prepared SAIP@NPs exhibited decent stability and a certain slow-release effect in phosphate buffer (PBS, pH 7.4). In vitro studies had shown that, due to the dual functions of transmembrane and targeting of iRGD peptide, SAIP@NPs exhibited strong binding to integrin αvß3, which was highly expressed on the membrane of MDA-MB-231 cells, improving the uptake capacity of tumor cells, inhibiting the rapid growth of tumor cells, and promoting tumor cell apoptosis. The results of animal experiments further proved that SAIP@NPs had longer residence time in tumor sites, stronger anti-tumor effect, and no obvious toxicity to major organs of experimental animals. Conclusion: The engineered SAIP@NPs exhibited superior functionalities including efficient membrane permeability, precise tumor targeting, and imaging, thereby significantly augmenting the therapeutic efficacy against breast cancer with a favorable safety profile.

Asunto(s)

Neoplasias de la Mama , Oro , Nanopartículas del Metal , Ratones Desnudos , Oligopéptidos , Dióxido de Silicio , Animales , Dióxido de Silicio/química , Femenino , Neoplasias de la Mama/tratamiento farmacológico , Humanos , Oligopéptidos/química , Oligopéptidos/farmacocinética , Oligopéptidos/farmacología , Oro/química , Oro/farmacocinética , Oro/farmacología , Ratones , Línea Celular Tumoral , Nanopartículas del Metal/química , Ensayos Antitumor por Modelo de Xenoinjerto , Ratones Endogámicos BALB C , Paclitaxel/química , Paclitaxel/farmacología , Paclitaxel/farmacocinética , Paclitaxel/administración & dosificación , Sistemas de Liberación de Medicamentos/métodos , Antineoplásicos/química , Antineoplásicos/farmacología , Antineoplásicos/farmacocinética , Portadores de Fármacos/química , Portadores de Fármacos/farmacocinética , Tamaño de la Partícula , Células MCF-7

RESUMEN

Materials with a soft tissue regenerative capacity can be produced using biopolymer scaffolds and nanomaterials, which allow injured tissue to recover without any side effects or limitations. Four formulations were prepared using polyvinyl alcohol (PVA) and chitosan (CS), with silicon dioxide nanoparticles (NPs-SiO2) incorporated using the freeze-drying method at a temperature of -50 °C. TGA and DSC showed no change in thermal degradation, with glass transition temperatures around 74 °C and 77 °C. The interactions between the hydroxyl groups of PVA and CS remained stable. Scanning electron microscopy (SEM) indicated that the incorporation of NPs-SiO2 complemented the freeze-drying process, enabling the dispersion of the components on the polymeric matrix and obtaining structures with a small pore size (between 30 and 60 µm) and large pores (between 100 and 160 µm). The antimicrobial capacity analysis of Gram-positive and Gram-negative bacteria revealed that the scaffolds inhibited around 99% of K. pneumoniae, E. cloacae, and S. aureus ATCC 55804. The subdermal implantation analysis demonstrated tissue growth and proliferation, with good biocompatibility, promoting the healing process for tissue restoration through the simultaneous degradation and formation of type I collagen fibers. All the results presented expand the boundaries in tissue engineering and regenerative medicine by highlighting the crucial role of nanoparticles in optimizing scaffold properties.

Asunto(s)

Quitosano , Liofilización , Nanopartículas , Alcohol Polivinílico , Dióxido de Silicio , Ingeniería de Tejidos , Andamios del Tejido , Quitosano/química , Alcohol Polivinílico/química , Dióxido de Silicio/química , Andamios del Tejido/química , Nanopartículas/química , Ingeniería de Tejidos/métodos , Animales , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Antibacterianos/farmacología , Antibacterianos/química , Medicina Regenerativa/métodos , Regeneración/efectos de los fármacos

RESUMEN

Silicon dioxide nanoparticles (SiO2-NPs) can be found in many products, such as composites, paints, ceramics, consumer products, and food additives. We recently demonstrated that via breastfeeding, SiO2-NPs transfer to the offspring's brain, interfering negatively with hippocampus development. In this work, we evaluated the protective effect of grape seed extract (GSE) against the adverse effects of SiO2-NPs. After delivery, animals were administered 25 mg/kg SiO2-NPs with/without GSE (300 mg/kg) for 20 days (from 2nd to 21st days post-delivery) by gavage. SiO2-NPs increased malondialdehyde concentration and decreased antioxidant activity in the offspring's hippocampi. The mean number of dark neurons (DNs) was significantly higher in the hippocampi of the SiO2-NPs group, whereas the mean number of DCX + cells was significantly lower than in the control group. The offspring in the SiO2-NPs groups had a weak cognitive performance in adulthood. Interestingly, these adverse effects of SiO2-NPs were alleviated in the GSE-treated groups. Therefore, GSE can attenuate the damaging effects of maternal exposure to SiO2-NPs during lactation.

Asunto(s)

Extracto de Semillas de Uva , Hipocampo , Nanopartículas , Dióxido de Silicio , Animales , Extracto de Semillas de Uva/farmacología , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Dióxido de Silicio/toxicidad , Femenino , Ratas , Masculino , Embarazo , Ratas Wistar , Efectos Tardíos de la Exposición Prenatal/prevención & control , Efectos Tardíos de la Exposición Prenatal/inducido químicamente , Síndromes de Neurotoxicidad/prevención & control , Antioxidantes/farmacología , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Fármacos Neuroprotectores/farmacología

RESUMEN

With environmental problems such as climate global warming, drought has become one of the major stress factors, because it severely affects the plant growth and development. Silicon dioxide nanoparticles (SiO2 NPs) are crucial for mitigating abiotic stresses suffered by plants in unfavorable environmental conditions and further promoting plant growth, such as drought. This study aimed to investigate the effect of different concentrations of SiO2 NPs on the growth of the Ehretia macrophylla Wall. seedlings under severe drought stress (water content in soil, 30-35%). The treatment was started by starting spraying different concentrations of SiO2 NPs on seedlings of Ehretia macrophyla, which were consistently under normal and severe drought conditions (soil moisture content 30-35%), respectively, at the seedling stage, followed by physiological and biochemical measurements, transcriptomics and metabolomics analyses. SiO2 NPs (100 mg·L-1) treatment reduced malondialdehyde and hydrogen peroxide content and enhanced the activity of antioxidant enzymes under drought stress. Transcriptomic analysis showed that 1451 differentially expressed genes (DEGs) in the leaves of E. macrophylla seedlings were regulated by SiO2 NPs under drought stress, and these genes mainly participate in auxin signal transduction and mitogen-activated protein kinase signaling pathways. This study also found that the metabolism of fatty acids and α-linolenic acids may play a key role in the enhancement of drought tolerance in SiO2 NP-treated E. macrophylla seedlings. Metabolomics studies indicated that the accumulation level of secondary metabolites related to drought tolerance was higher after SiO2 NPs treatment. This study revealed insights into the physiological mechanisms induced by SiO2 NPs for enhancing the drought tolerance of plants.

RESUMEN

Herein, a high-performance sucrose-tannin bio-based adhesive is developed based on consisting of oxidized sucrose (OS), tannin acid (TA), SiO2 nanoparticles and 2,2'-disulfanediylbis (ethan-1-amine) (DBA) by a facile chemical cross-linking strategy. The OS-TA and OS-TA@SiO2 bio-based adhesives are characterized by XPS, FTIR, and 13C NMR, while the bonding performance is also investigated using shear strength test. Results show that the optimal formulation of OS-TA bio-based adhesive is a 2:1:1 mass ratio for OS: TA: DBA. When the mass fraction of SiO2 is 15 % and the solid content of main components is 50 %, the OS-TA@SiO2 bio-based adhesive has excellent bonding strength. Relative to OS-TA, the wet bonding strength of the OS-TA@SiO2 enhanced from 1.16 MPa to 1.85 MPa, while the dry bonding strength improved from 1.90 MPa to 2.50 MPa. The wood failure rate of the plywood fabricated by using the OS-TA@SiO2 bio-based adhesive reaches 80 %. Therefore, relying on the high flexibility of dynamic disulfide bonds, adding SiO2 nanoparticles into the adhesive system can facilitate greatly the mechanical interlocking effect and make the chemical cross-linking network more compact through the synergistic chemical interactions. This work provides new insights into producing green and renewable bio-based wood adhesives using sucrose and tannin.

Asunto(s)

Sacarosa , Taninos , Taninos/química , Adhesivos/química , Bases de Schiff , Dióxido de Silicio , Disulfuros

RESUMEN

Engineered nanoparticles (NPs) have recently attracted a lot of attention after being tested in various agricultural plants. This paper reports the green synthesis of CeO2 NPs and SiO2 NPs from leaf extracts of Nyctanthes arbor-tristis. The physical characteristics of the produced nanoparticles were then determined using UV-visible spectroscopy, transmission electron microscopy (TEM), fluorescence spectroscopy, and Fourier transform infrared spectroscopy (FTIR). Furthermore, the interaction effects of cerium oxide NPs (C1, C2, and C3) and silicon dioxide NPs (S1, S2, and S3) at 10 mg/L on blackgram (Vigna mungo L.) were evaluated. CeO2 and SiO2 NPs treatments enhanced the growth performance of the plants by causing a decrease in superoxide radical (SOR) and H2O2 via improving antioxidant enzymes. These findings imply that the size and shape of CeO2 and SiO2 NPs provide defense against oxidative damage to the blackgram.

Asunto(s)

Cerio , Nanopartículas del Metal , Nanopartículas , Vigna , Antioxidantes , Dióxido de Silicio , Peróxido de Hidrógeno , Nanopartículas/química , Espectroscopía Infrarroja por Transformada de Fourier , Cerio/química , Nanopartículas del Metal/química

RESUMEN

Copper (Cu) is an essential micronutrient for plants; however, the excessive accumulation of Cu due to various anthropogenic activities generates progressive pollution of agricultural land and that causes a major constraint for crop production. Excess Cu (80 mg kg-1) in the soil diminished growth and biomass, photosynthetic efficiency and essential oil (EO) content in Mentha arvensis L., while amplifying the antioxidant enzyme's function and reactive oxygen species (ROS) production. Therefore, there is a pressing need to explore effective approaches to overcome Cu toxicity in M. arvensis plants. Thus, the present study unveils the potential of foliar supplementation of two distinct forms of silicon dioxide nanoparticles (SiO2 NPs) i.e., Aerosil 200F and Aerosil 300 to confer Cu stress tolerance attributes to M. arvensis. The experiment demonstrated that applied forms of SiO2 NPs (120 mg L-1), enhanced plants' growth and augmented the photosynthetic efficiency along with the activities of CA (carbonic anhydrase) and NR (nitrate reductase), however, the effects were more accentuated by Aerosil 200F application. Supplementation of SiO2 NPs also exhibited a beneficial effect on the antioxidant machinery of Cu-disturbed plants by raising the level of proline and total phenol as well as the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutathione reductase (GR), thereby lowering ROS and electrolytic leakage (EL). Interestingly, SiO2 NPs supplementation upscaled EO production in Cu-stressed plants with more pronounced effects received in the case of Aerosil 200F over Aerosil 300. We concluded that the nano form (Aerosil 200F) of SiO2 proved to be the best in improving the Cu-stress tolerance in plants.

Asunto(s)

Nanopartículas , Aceites Volátiles , Antioxidantes/metabolismo , Cobre/toxicidad , Especies Reactivas de Oxígeno , Dióxido de Silicio/toxicidad , Aceites Volátiles/toxicidad , Nanopartículas/toxicidad , Homeostasis , Peróxido de Hidrógeno , Estrés Oxidativo

RESUMEN

The wide application of silicon dioxide nanoparticles (SiO2NPs) has raised concerns about their harmful effects on reproduction. The purpose of this research was to investigate the toxic effects and the possible mechanisms by which SiO2NPs affect decidualization and pregnancy progression. We found that SiO2NPs could inhibit decidualization, both in mice and in human endometrial stromal cells (HESCs). Embryo resorption was also evident in mice treated with SiO2NPs. When HESCs were treated with SiO2NPs, decidualization was inhibited and there was an increase in intracellular lysosomes and autophagosomes as well as the blockage of autophagic flux. Interestingly, a reduction of autophagosome accumulation via 3-methyladenine (3MA) significantly restored the decidualization of HESCs. In summary, our results indicate that SiO2NPs can affect embryo survival by impairing decidualization through a dysfunctional autophagic process.

Asunto(s)

Decidua , Nanopartículas , Embarazo , Femenino , Humanos , Ratones , Animales , Dióxido de Silicio/toxicidad , Pérdida del Embrión , Autofagia , Nanopartículas/toxicidad , Células del Estroma

RESUMEN

Background: The ultimate goal of endodontic therapy is to eliminate all microorganisms present inside root canal and thereby sealing all the possible communicating pathways between pulpal and periradicular tissues, which prevents all the factors that cause recontamination and reinfection of the root canal system. If endodontic treatment fails, next approach is surgical endodontics. Bioceramics are recently introduced materials specifically designed for their potential use in medical field and dentistry. Aim: To evaluate and compare the push-out bond strength of mineral trioxide aggregate (MTA) by adding titanium dioxide (TiO2), silver, and silicon dioxide nanoparticles. Materials and Methods: Totally, 60 single-rooted human teeth were used. Middle third of the root was sectioned to obtain 2-mm thick root section. Acrylic was adapted to the section to obtain disks of 5 mm diameter and 2 mm thickness. Canal was prepared by GG Drill. Samples were divided into four groups of 15 each (n = 15): • Group I (control): MTA • Group II: MTA + TiO2 nanoparticles. • Group III: MTA + silver nanoparticles. • Group IV: MTA + silicon dioxide nanoparticles. The cement mixture was compacted into the canal. Samples were subjected to push-out bond strength using universal testing machine. Statistical Analysis Used: The data were analyzed statistically by analysis of variance and post hoc comparison by Tukey's t-test. Results: The highest push-out bond strength was shown by Group II (MTA with TiO2 nanoparticles), followed by Group III (MTA with silver nanoparticles) and Group I (MTA control group). The lowest push-out bond strength was shown by Group III (MTA with silicon dioxide nanoparticles). Conclusions: TiO2 and silver nanoparticles when added into MTA lead to an increase in push-out bond strength of MTA.

RESUMEN

Three types of modified silicon dioxide nanoparticles (SiO2, 10−20 nm) with additives of epoxy, silane and amino groups, used independently and in combination with the entomopathogenic bacteria Bacillus thuringiensis subsp. morrisoni and fungus Metarhizium robertsii were tested against Colorado potato beetle (Leptinotarsa decemlineata) and cabbage beetles (Phyllotreta spp.). All three nanoparticles were found to have an entomocidal effect on Colorado potato beetle larvae and crucifer flea beetles when ingested. Increased susceptibility of insects to B. thuringiensis or M. robertsii blastospores and their metabolites was shown after exposure to the modified silicon dioxide nanoparticles. The potential of modified silicon dioxide nanoparticles to enhance the efficiency of biopesticides based on the bacteria B. thuringiensis and fungi M. robertsii is considered in the paper.

RESUMEN

Silicon dioxide nanoparticles (SiO2-NPs) are among the most widely used nanoparticles because of their chemical-physical properties. Since most brain maturation occurs in the neonatal period in humans and many mammals, it is important to understand how NPs may affect this process. This study tested the hypothesis that SiO2-NPs from treated dams could affect the hippocampus of neonatal rats during lactation. Twenty-four pregnant rats, after delivery, were divided into three groups of control, SiO2-NPs (25 mg/kg) and SiO2-NPs (100 mg/kg). The rats were treated from 2nd to 21st days post-delivery by gavage and the effects of these NPs were evaluated in the offspring's hippocampi to reveal the effects of maternal exposure to SiO2-NPs during lactation on the offspring's hippocampi. The offspring in the SiO2-NPs groups had higher malondialdehyde concentration and lower antioxidant activity in the hippocampi than the non-treated control group. The mean number of doublecortin positive (DCX+) cells and synaptophysin expression in the hippocampi of the SiO2-NPs groups were significantly lower than the control group, whereas the mean number of dark neurons was significantly higher. Also, animals in the SiO2-NPs groups had a weak cognitive performance in adulthood. In conclusion, maternal exposure to SiO2-NPs via breastfeeding could affect offspring's hippocampal neurogenesis and synaptogenesis, leading to impaired cognitive performance.

Asunto(s)

Hipocampo/efectos de los fármacos , Hipocampo/crecimiento & desarrollo , Exposición Materna/efectos adversos , Nanopartículas/toxicidad , Neurogénesis/efectos de los fármacos , Dióxido de Silicio/toxicidad , Animales , Femenino , Lactancia , Embarazo , Ratas

RESUMEN

The increasingly widespread use of engineered nanoparticles in medical, industrial, and food applications has raised concerns regarding their potential toxicity to humans and the environment. Silicon dioxide nanoparticles (SiO2 NPs), which have relatively low direct toxicity, have been increasingly applied in both consumer products and biomedical applications, leading to significantly higher exposure for humans and the environment. We carried out a toxicity assessment of SiO2 NPs using the common water flea D. magna by focusing on physiological and behavioral indicators such as heart rate, swimming performance, and growth. Exposure to SiO2 NPs did not produce acute or chronic toxicity at limited concentrations (<100 µg/mL), but did have statistically significant negative effects on heart rate, swimming distance, and body size. The use of fluorescein isothiocyanate in a silica matrix allowed the tracing and visualization of clear SiO2 NP accumulation in D. magna, which was confirmed by ICP-MS. Although exposure to SiO2 NPs seemed to affect cardiac and swimming performance, such end-point experiments may be insufficient to fully understand the toxicity of these nanoparticles. However, the physiological and behavioral changes shown here suggest potential adverse effects on the aquatic environment by substances previously considered nontoxic.

RESUMEN

Nano-SiO2 is increasingly used in diagnostic and biomedical research because of its ease of production and relatively low cost and which is generally regarded as safe and has been approved for use as a food or animal feed ingredient. Although recent literature reveals that nano-SiO2 may present toxicity and DNA damage, however, the underlying mechanism remains poorly understood. Since in previous studies, we found that nano-SiO2 treatment down-regulated the expression of the poly(ADP-ribose) polymerases-1 (PARP-1), a pivotal DNA repair gene, in human HaCaT cells and PAPR-1 knockdown can aggravate DNA damage induced by nano-SiO2. Therefore, we speculate whether PARP-1 overexpression can protect DNA from damage induced by nano-SiO2. However, our data demonstrated that overexpression of PARP-1 in HaCaT cells slightly enhanced the cellular proliferation of undamaged cells, when compared with both empty vector control cells and parental cells, but had drastic consequences for cells treated with nano-SiO2. The PARP-1 overtransfected cells were sensitized to the cytotoxic effects and DNA damage of nano-SiO2 compared with control parental cells. Meanwhile, flow cytometric analysis of nano-SiO2 stimulated poly(ADP-ribose) synthesis revealed consistently larger fractions of cells positive for this polymer in the PARP-1 overexpression cells than in control clones. Combining our previous research on PARP-1 knockdown HaCaT cells, we hypothesize that an optimal level of cellular poly(ADP-ribose) accumulation exists for the cellular recovery from DNA damage.

RESUMEN

BACKGROUND: Silicon dioxide nanoparticles (SiO2NPs) are widely used as additive in the food industry with controversial health risk. Gut microbiota is a new and hot topic in the field of nanotoxicity. It also contributes a novel and insightful view to understand the potential health risk of food-grade SiO2NPs in children, who are susceptible to the toxic effects of nanoparticles. METHODS: In current study, the young mice were orally administrated with vehicle or SiO2NPs solution for 28 days. The effects of SiO2NPs on the gut microbiota were detected by 16S ribosomal RNA (rRNA) gene sequencing, and the neurobehavioral functions were evaluated by open field test and Morris water maze. The level of inflammation, tissue integrity of gut and the classical indicators involved in gut-brain, gut-liver and gut-lung axis were all assessed. RESULTS: Our results demonstrated that SiO2NPs significantly caused the spatial learning and memory impairments and locomotor inhibition. Although SiO2NPs did not trigger evident intestinal or neuronal inflammation, they remarkably damaged the tissue integrity. The microbial diversity within the gut was unexpectedly enhanced in SiO2NPs-treated mice, mainly manifested by the increased abundances of Firmicutes and Patescibacteria. Intriguingly, we demonstrated for the first time that the neurobehavioral impairments and brain damages induced by SiO2NPs might be distinctively associated with the disruption of gut-brain axis by specific chemical substances originated from gut, such as Vipr1 and Sstr2. Unapparent changes in liver or lung tissues further suggested the absence of gut-liver axis or gut-lung axis regulation upon oral SiO2NPs exposure. CONCLUSION: This study provides a novel idea that the SiO2NPs induced neurotoxic effects may occur through distinctive gut-brain axis, showing no significant impact on either gut-lung axis or gut-liver axis. These findings raise the exciting prospect that maintenance and coordination of gastrointestinal functions may be critical for protection against the neurotoxicity of infant foodborne SiO2NPs.

Asunto(s)

Encéfalo/efectos de los fármacos , Microbioma Gastrointestinal/efectos de los fármacos , Microbioma Gastrointestinal/fisiología , Nanopartículas/química , Dióxido de Silicio/farmacología , Animales , Microbioma Gastrointestinal/genética , Inflamación , Hígado/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos C57BL , Neuronas , Dióxido de Silicio/química

RESUMEN

Drought tolerant plant growth-promoting rhizobacteria (PGPR) can confer drought tolerance in plants, when inoculated, and this effect can be more pronounced by their combined application with silicon oxide nanoparticles (SiO2 NPs). In this research, drought-tolerant and plant growth-promoting rhizobacterial strains were isolated from the rhizospheric soil of wheat plants growing in the arid region of Pakistan. Out of 30 isolated strains, three rhizobacterial strains were selected based on their drought tolerance, higher phytohormones (indole acetic acid (IAA), abscisic acid (ABA), and cytokinin (CK), and osmolyte (proline and sugar) production ability. These strains were identified as Bacillus sp. Azospirillum lipoferum and Azospirillum brasilense by 16S rRNA sequencing and accession numbers (MT482404, MT742664, and MT 742666, respectively) were obtained. Inoculation of these strains, alone and in combination, improved the germination attributes of wheat seeds under drought stress conditions. However, the combination of all three bacterial strains gave the best results. SiO2 NPs were prepared from silicon dioxide and characterized by scanning electron microscopy (SEM), Energy dispersive X-rays pattern (EDX), and UV-visible spectrum. The effect of SiO2 NPs was also tested on wheat seeds under drought stress and it was observed that SiO2 NPs (150 mg/L) create pronounced drought ameliorative potential in wheat seedlings. In the pot experiment, the combined application of SiO2 NPs and PGPR exhibited a synergistic role and improved the growth and yield of wheat. The interaction between SiO2 NPs and bacterial combination improved biomass (fresh and dry weight), and chlorophyll-a, b content by 138.78%, 65.70%, 128.57%, and 283.33% respectively as compared to untreated but drought exposed plants. They also improved relative water content (71.66%), gas exchange attributes, increased nutrients uptake, and osmolytes production of wheat. Up-regulation of antioxidant enzymes; superoxide dismutase (60.49%), peroxidase (55.99%), and catalase (81.69%) was also observed. This research work suggested that the application of SiO2 NPs and PGPR strains induced drought tolerance in wheat by modulating different physiological and metabolic processes in plants which ultimately improved the growth and yield of wheat under drought stress.

Asunto(s)

Sequías , Triticum , Desarrollo de la Planta , Raíces de Plantas , ARN Ribosómico 16S , Dióxido de Silicio

RESUMEN

Silicon dioxide nanoparticles (SiO2 NPs) are extensively used in cosmetics, food, and drug delivery. The main mechanism of SiO2 NPs toxicities depends on oxidative stress. Ginseng (Panax ginseng Meyer) is used in various medicinal applications because of its antioxidant efficiency. Therefore, the present study was carried out to investigate the possible combated role of ginseng against SiO2 NPs toxicity in rat liver. Thirty-five male rats (160-180 g) were allocated into five groups of seven rats each, randomly. The first group was used as a control while groups 2, 3, 4, and 5 were treated orally with ginseng (Gin; 75 mg/kg, 1/10 LD50 ), SiO2 NPs, (200 mg/kg, 1/10 LD50 ), Gin + SiO2 NPs (protection group), and SiO2 NPs + Gin (therapeutic group) for 5 weeks, respectively. Treatment with SiO2 NPs increased lipid peroxidation, liver function enzymes, and decreased antioxidant enzymes (SOD, CAT, GPx, GST) activity and non-enzymatic antioxidant (GSH) level. SiO2 NPs administration motivated liver apoptosis as revealed by the upregulation of the apoptotic genes, Bcl2-associated x protein (Bax), and Beclin 1 and downregulation of the anti-apoptotic gene, B-cell lymphoma 2 (Bcl2) as well as increase in DNA damage. Also, SiO2 NPs administration caused inflammation as indicated by upregulation of the inflammation-related genes (interleukin 1 beta [IL1ß], tumor necrosis factor-alpha [TNFα], nuclear factor kappa B [NFκB], cyclooxygenase 2 [Cox2], transforming growth factor-beta 1 [TGFß1]) as well as cell cycle arrest in the G0/G1 phase of liver cells. Moreover, histopathological examination proved the biochemical and molecular perturbations occurred due to SiO2 NPs toxicity. On the other hand, ginseng caused a significant modulation on the deleterious effects induced by SiO2 NPs in rat liver. In conclusion, ginseng has a potent preventive effect than the therapeutic one and might be used in the treatment of SiO2 NPs hepatotoxicity.

Asunto(s)

Nanopartículas , Panax , Animales , Apoptosis , Daño del ADN , Inflamación/inducido químicamente , Masculino , Nanopartículas/toxicidad , Estrés Oxidativo , Ratas , Dióxido de Silicio/toxicidad

RESUMEN

Objective: To investigate the protective and therapeutic role of ginseng against silicon dioxide nanoparticles (SiO

RESUMEN

Dissipative particle dynamics (DPD) simulations was adopted to investigate the modification of polyvinylidene fluoride (PVDF) membrane by adding zwitterionic polymer brush poly(sulfobetaine methacrylate)- tetraethyl orthosilicate (PSBMA-TEOS) grafted silicon nanoparticles (SNPs) to the casting solution. The effects of polymer concentration and grafting architecture (PSBMA length and SNPs grafting ratio) on membrane morphology are discussed. When the polymer concentration reaches 40%, part of the SNPs is embedded in the membrane; the optimal polymer concentration is around 25-30%. In the SNPs system with the grafting ratio of 1, some SNPs are eluted into solution during phase separation. Compared with different grafting architectures, M8-5, M10-5 and M12-5 system (Mx-y, where x represents the length of the zwitterionic polymer brush and y represents the grafting ratio of the silica nanoparticles) exhibited stable membrane morphologies. This work can provide guidance for the design and modification of organic-inorganic composite membrane and help understand the distribution of modified materials on the membrane surface.

RESUMEN

Morphological analysis of the respiratory tract of Wistar rats was performed after a single parenteral administration of 12-nm silicon dioxide nanoparticles (1 ml, 2 mg/ml, intravenously) was performed. On day 21 and in 2, 4, and 6 months after the administration of nanoparticles, the development of macrophage infiltration in the interstitium of the respiratory tract was demonstrated by histological and immunohistochemical methods. The pool of alveolar macrophages increased in 4 months after administration (p=0.004) and returned to the control values in 6 months. The number of mast cells did not significantly change at all stages of the experiment. Connective tissue remodeling in the interstitium of the respiratory tract was not observed throughout the observation period.

Asunto(s)

Pulmón/efectos de los fármacos , Macrófagos Alveolares/efectos de los fármacos , Mastocitos/efectos de los fármacos , Nanopartículas/química , Dióxido de Silicio/farmacología , Animales , Recuento de Células , Movimiento Celular/efectos de los fármacos , Histocitoquímica , Inyecciones Intravenosas , Pulmón/patología , Macrófagos Alveolares/patología , Masculino , Mastocitos/patología , Nanopartículas/ultraestructura , Tamaño de la Partícula , Ratas , Ratas Wistar

RESUMEN

Species of Hyoscyamus are rich sources of medicinally important tropane alkaloids, which have anticholinergic, antispasmodic and sedative effects and are competitive inhibitors of acetylcholine. The application of nanotechnology and nanomaterials for elicitation is rapidly expanding and recent research indicates that silicon dioxide nanoparticles (SiO2 NPs) can be used as an efficient elicitor to increase the production of hyoscyamine and scopolamine in Hyoscyamus species. Thus, in this work, the effect of SiO2 NPs (0, 25, 50, 100 and 200 mg L-1) with two treatment times (24 and 48 h) on the growth rate, total phenol and flavonoid content (TPC, TFC), antioxidant enzyme activity, tropane alkaloid yield and pmt (putrescine N-methyltransferase) and h6h (hyoscyamine 6-hydroxylase) gene expression levels in hairy roots of two Hyoscyamus species (H. reticulatus and H. pusillus) was investigated. The highest TPC and TFC accumulation was obtained in H. reticulatus elicited by SiO2 NPs (100 and 200 mg L-1), respectively, at 24 h of treatment. High-performance liquid chromatography (HPLC) revealed the highest amount of hyoscyamine (140.15 µg g-1 FW) and scopolamine (67.71 µg g-1 FW) accumulated in H. reticulatus transformed roots treated with 100 mg L-1 SiO2 NPs at 24 h, with a respective increase of 1212% and 272% compared to non-treated roots. In H. pusillus, the highest hyoscyamine (7.42 µg g-1 FW) and scopolamine (15.56 µg g-1 FW) production (about 82% and 241% higher, respectively, compared to the lowest amounts) was achieved with 25 and 100 mg L-1 SiO2 NPs, respectively, at 48 h of treatment. Semi-quantitative RT-PCR analysis determined the highest expression level of pmt and h6h genes in H. reticulatus transformed roots supplemented with 100 mg L-1 SiO2 NPs.

Asunto(s)

Hyoscyamus/metabolismo , Nanopartículas , Raíces de Plantas/metabolismo , Dióxido de Silicio/farmacología , Tropanos/metabolismo , Regulación de la Expresión Génica de las Plantas , Hyoscyamus/genética , Raíces de Plantas/genética , Técnicas de Cultivo de Tejidos