RESUMEN

Aluminum alloy tubes are widely used in various industries because of their excellent performance. Up to now, when the tube is bent, the elastoplastic deformation evolution mechanism of the cross-section has not been clear, and no direct analytical proof has been found. In this paper, based on the bilinear material model assumption, a new mechanical model of tube plane bending deformation is constructed. The analytical model can describe in detail the evolution mechanism of elastic-plastic deformation on the cross-section of the tube after bending deformation, the position of the elastic-plastic boundary, the position of the radius of the strain neutral layer, and the relationship between the bending moment over the section and the bending radius. According to this model, the deformation law of the tube cross-section during bending is elucidated. The results are as follows: (1) the deformation evolution of the cross-section of the bending deformed tube calculated by the analytical model is in good agreement with the finite element model (FEM) of pure bending. (2) By comparing the results of the analytical model with FEM results, and the processing test of the self-designed four-axis free bending forming tube bender, the bending moments are in good agreement. (3) Compared with the bending moments calculated by several other analytical models of tube bending, this model has a relatively small deviation value.

RESUMEN

With the ongoing developments in laser scanning technology, applications for describing tunnel deformation using rich point cloud data have become a significant topic of investigation. This study describes the independently developed CNU-TS-2 mobile tunnel monitoring system for data acquisition, which has an electric system to control its forward speed and is compatible with various laser scanners such as the Faro and Leica models. A comparison with corresponding data acquired by total station data demonstrates that the data collected by CNU-TS-2 is accurate. Following data acquisition, the overall and local deformation of the tunnel is determined by denoising and 360° deformation analysis of the point cloud data. To enhance the expression of the analysis results, this study proposes an expansion of the tunnel point cloud data into a two-dimensional image via cylindrical projection, followed by an expression of the tunnel deformation through color difference to visualize the deformation. Compared with the three-dimensional modeling method of visualization, this method is easier to implement and facilitates storage. In addition, it is conducive to the performance of comprehensive analysis of problems such as water leakage in the tunnel, thereby achieving the effect of multiple uses for a single image.

RESUMEN

The optical disector, a three-dimensional counting frame or probe in stereology, is often positioned in the middle (depth) of a thick section for unbiased nuclear counting. Using 30-40 µm thick methacrylate or paraffin sections for nuclear counting of neurons with the optical disector, however, some studies showed markedly higher nuclear densities at 10% of the section thickness near the top or bottom surface of the section, suggestive of deformation of section along its z axis and thus affecting the number estimation. To verify the findings, this study obtained two sets of 12-14 methacrylate sections (average thicknesses 21.7 and 29.4 µm) and two sets of 12 paraffin sections (average thicknesses 13.8 and 29.2 µm) from mature rat testes. Each section was used to count round spermatid nuclei in the seminiferous epithelium densely packed with the cells, using 3-4 consecutive disectors placed vertically (along the z axis of the section) from the top surface of the section, through the whole section thickness (two sets of methacrylate and paraffin sections) or in 80-83% of the thickness (other sections). The results demonstrated that, overall, there were no considerable nonuniform changes of the nuclear densities along the z axis of the sections.

RESUMEN

Stereology is a collection of methods which makes it possible to produce interpretations about actual three-dimensional features of objects based on data obtained from their two-dimensional sections or images. Quantitative morphological studies of the central nervous system have undergone significant development. In particular, new approaches known as design-based methods have been successfully applied to neuromorphological research. The morphology of macroscopic and microscopic structures, numbers of cells in organs and structures, and geometrical features such as length, volume, surface area and volume components of the organ concerned can be estimated in an unbiased manner using stereological techniques. The most practical and simplest stereological method is the fractionator technique, one of the most widely used methods for total particle number estimation. This review summarizes fractionator methods in theory and in practice. The most important feature of the methods is the simplicity of its application and underlying reasoning. Although there are three different types of the fractionator method, physical, optical and isotropic (biochemical), the logic underlying its applications remains the same. The fractionator method is one of the strongest and best options among available methods for estimation of the total number of cells in a given structure or organ. The second part of this review focuses on recent developments in stereology, including how to deal with lost caps, with tissue section deformation and shrinkage, and discusses issues of calibration, particle identification, and the role of stereology in the era of a non-histological alternative to counting of cells, the isotropic fractionator (brain soup technique).

Asunto(s)

RESUMEN

The local deformation and variations in section thickness are studied in 100-µm thick vibratome sections of well-fixed human brain tissue. During processing, including drying on glass slides, the section thickness is reduced to less than half, but close to the edges there is less shrinkage of the section thickness. Close to both surfaces there is a pronounced reduction in the number of neuronal nucleoli. At the scale of the original section, the upper 15 µm and the lower 10 µm are depleted. The loss is most pronounced at the upper surface, which is unprotected during processing. In the central 70% of the section height, where one would ordinarily use an optical disector for sampling, there is no indication of non-uniform shrinkage. The simplest explanation for the observed loss of nucleoli is that all cells opened by the knife may lose their nuclei across an unprotected section surface. The observations do not generalize to other tissues and other preparation techniques, but illustrate the magnitude of some of the problems for uniform sampling and unbiased estimation in very thick sections. The uniform optical disector sampling of nucleoli in thick sections, as opposed to that of cell nuclei, raises a special problem, which is discussed briefly.