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Drought and salinity stress reduce root hydraulic conductivity of plant seedlings, and melatonin application positively mitigates stress-induced damage. However, the underlying effect of melatonin priming on root hydraulic conductivity of seedlings under drought-salinity combined remains greatly unclear. In the current report, we investigated the influence of seeds of three wheat lines' 12 h priming with 100 µM of melatonin on root hydraulic conductivity (Lpr) and relevant physiological indicators of seedlings under PEG, NaCl, and PEG + NaCl combined stress. A previous study found that the combined PEG and NaCl stress remarkably reduced the Lpr of three wheat varieties, and its value could not be detected. Melatonin priming mitigated the adverse effects of combined PEG + NaCl stress on Lpr of H4399, Y1212, and X19 to 0.0071 mL·h-1·MPa-1, 0.2477 mL·h-1·MPa-1, and 0.4444 mL·h-1·MPa-1, respectively, by modulating translation levels of aquaporin genes and contributed root elongation and seedlings growth. The root length of H4399, Y1212, and X19 was increased by 129.07%, 141.64%, and 497.58%, respectively, after seeds pre-treatment with melatonin under PEG + NaCl combined stress. Melatonin -priming appreciably regulated antioxidant enzyme activities, reduced accumulation of osmotic regulators, decreased levels of malondialdehyde (MDA), and increased K+ content in stems and root of H4399, Y1212, and X19 under PEG + NaCl stress. The path investigation displayed that seeds primed with melatonin altered the modification of the path relationship between Lpr and leaf area under stress. The present study suggested that melatonin priming was a strategy as regards the enhancement of root hydraulic conductivity under PEG, NaCl, and PEG + NaCl stress, which efficiently enhanced wheat resistant to drought-salinity stress.

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Alterations in root hydraulics in response to varying moisture conditions remain a subject of debate. In our investigation, we subjected common reeds (Phragmites australis) to a 45-day treatment with four distinct soil moisture levels. The findings unveiled that, in response to drought stress, the total root length, surface area, volume, and average diameter exhibited varying degrees of reduction. Anatomically, drought caused a reduction in root diameter (RD), cortex thickness (CT), vessel diameter (VD), and root cross-sectional area (RCA). A decrease in soil moisture significantly reduced both whole- and single-root hydraulic conductivity (Lpwr, Lpsr). The total length, surface area, volume, and average diameter of the reed root system were significantly correlated with Lpwr, while RD, CT, and RCA were significantly correlated with Lpsr. A decrease in soil moisture content significantly influenced root morphological and anatomical characteristics, which, in turn, altered Lpr, and the transcriptome results suggest that this may be associated with the variation in the expression of abscisic acid (ABA) and aquaporins (AQPs) genes. Our initial findings address a gap in our understanding of reed hydraulics, offering fresh theoretical insights into how herbaceous plants respond to external stressors.

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Water shortages and crop responses to drought and salt stress are related to the efficient use of water resources and are closely related to food security. In addition, PEG or NaCl stress alone affect the root hydraulic conductivity (Lpr). However, the effects of combined PEG and NaCl stress on Lpr and the differences among wheat varieties are unknown. We investigated the effects of combined PEG and NaCl stress on the root parameters, nitrogen (N) and carbon content, antioxidant enzymes, osmotic adjustment, changes in sodium and potassium, and root hydraulic conductivity of Yannong 1212, Heng 4399, and Xinmai 19. PEG and NaCl stress appreciably decreased the root length (RL), root surface area (RS), root volume (RV), K+ and N content in shoots and roots, and Lpr of the three wheat varieties, while the antioxidant enzyme activity, malondialdehyde (MDA), osmotic adjustment, nonstructural carbon and Na+ content in shoots and roots, etc., remarkably remained increased. Furthermore, the root hydraulic conductivity had the greatest positive association with traits such as RL, RS, and N and K+ content in the shoots of the three wheat varieties. Moreover, the RL/RS directly and actively determined the Lpr, and it had an extremely positive effect on the N content in the shoots of wheat seedlings. Collectively, most of the root characteristics in the wheat seedlings decreased under stress conditions, resulting in a reduction in Lpr. As a result, the ability to transport nutrients-especially N-from the roots to the shoots was affected. Therefore, our study provides a novel insight into the physiological mechanisms of Lpr.

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Climate change is leading to combined drought and high temperature stress in many areas, drastically reducing crop production, especially for high-water-consuming crops such as maize. This study aimed to determine how the co-inoculation of an arbuscular mycorrhizal (AM) fungus (Rhizophagus irregularis) and the PGPR Bacillus megaterium (Bm) alters the radial water movement and physiology in maize plants in order to cope with combined drought and high temperature stress. Thus, maize plants were kept uninoculated or inoculated with R. irregularis (AM), with B. megaterium (Bm) or with both microorganisms (AM + Bm) and subjected or not to combined drought and high temperature stress (D + T). We measured plant physiological responses, root hydraulic parameters, aquaporin gene expression and protein abundances and sap hormonal content. The results showed that dual AM + Bm inoculation was more effective against combined D + T stress than single inoculation. This was related to a synergistic enhancement of efficiency of the phytosystem II, stomatal conductance and photosynthetic activity. Moreover, dually inoculated plants maintained higher root hydraulic conductivity, which was related to regulation of the aquaporins ZmPIP1;3, ZmTIP1.1, ZmPIP2;2 and GintAQPF1 and levels of plant sap hormones. This study demonstrates the usefulness of combining beneficial soil microorganisms to improve crop productivity under the current climate-change scenario.

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Salt stress reduces plant water flow during day and night. It is not known to which extent root hydraulic properties change in parallel. To test this idea, hydroponically grown wheat plants were grown at four levels of salt stress (50, 100, 150 and 200 mM NaCl) for 5-8d before harvest (d14-18) and subjected to a range of analyses to determine diurnal changes in hydraulic conductivity (Lp) at cell, root and plant level. Cell pressure probe analyses showed that the Lp of cortex cells was differentially affected by salt stress during day and night, and that the response to salt stress differed between the main axis of roots and lateral roots. The Aquaporin (AQP) inhibitor H2 O2 reduced Lp to a common, across treatments, level as observed in salt-stressed plants during the night. Analyses of transpiring plants and exuding root systems provided values of root Lp which were in the same range as values modeled based on cell-Lp. The results can best be explained through a change in root Lp in response to salt stress and day/night, which results from an altered activity of AQPs. qPCR gene expression analyses point to possible candidate AQP isoforms.

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The root system in plants absorbs water and minerals. However, the relationship among root size, yield, and water use efficiency (WUE) is controversial. Two pot experiments were conducted to explore these relationships by using two maize varieties with contrasting root sizes and reducing the root-shoot ratio (R/S) through root pruning to eliminate genotypic effects. Maize plants were grown in an open rainout shelter under both water-sufficient and deficient conditions. Yield-related parameters, root hydraulic conductivity (Lpr), and WUE were determined. The results showed that the small root variety (XY) has a higher yield and WUE compared to large root variety (QL) under both soil moisture conditions, likely related to the higher Lpr of XY. XY also had a higher leaf water potential than QL under drought stress, indicating that small root system could provide enough water to the shoot. Further pot experiment showed that both small and large root pruning on QL (cut off about 1/5 roots, RP1; and cut off about 1/3 roots, RP2, respectively) improved WUE and Lpr, and the RP1 yield increased by 12.9% compared to the control under well-watered conditions. Root pruning decreased transpiration and increased photosynthesis. Thus, this study reveals that it is possible to increase water absorption, yield, and WUE by reducing R/S in modern maize varieties, which may be important for the future breeding of new cultivars suitable for arid regions.

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In this study, a first experiment was conducted with the objective of determining how drought stress alters the radial water flow and physiology in the whole maize nested association mapping (NAM) population and to find out which contrasting maize lines should be tested in a second experiment for their responses to drought in combination with an arbuscular mycorrhizal (AM) fungus. Emphasis was placed on determining the role of plant aquaporins and phytohormones in the responses of these contrasting maize lines to cope with drought stress. Results showed that both plant aquaporins and hormones are altered by the AM symbiosis and are highly involved in the physiological responses of maize plants to drought stress. The regulation by the AM symbiosis of aquaporins involved in water transport across cell membranes alters radial water transport in host plants. Hormones such as IAA, SA, ABA and jasmonates must be involved in this process either by regulating the own plant-AM fungus interaction and the activity of aquaporins, or by inducing posttranscriptional changes in these aquaporins, which in turns alter their water transport capacity. An intricate relationship between root hydraulic conductivity, aquaporins and phytohormones has been observed, revealing a complex network controlling water transport in maize roots.

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Root systems are an important component of plants that impact crop water-use efficiency (WUE) and yield. This study examined the effects of root pruning on maize yield, WUE, and water uptake under pot and hydroponic conditions. The pot experiment showed that root pruning significantly decreased root/shoot ratio. Both small root pruning (cut off about 1/5 of the root system, RP1) and large root pruning (cut off about 1/3 of the root system, RP2) improved WUE and root hydraulic conductivity (Lpr) in the residual root system. Compared with that in the un-cut control, at the jointing stage, RP1 and RP2 increased Lpr by 43.9% and 31.5% under well-watered conditions and 27.4% and 19.8% under drought stress, respectively. RP1 increased grain yield by 12.9% compared with that in the control under well-watered conditions, whereas both pruning treatments did not exhibit a significant effect on yield under drought stress. The hydroponic experiment demonstrated that root pruning did not reduce leaf water potential but increased residual root hydraulic conductivity by 26.2% at 48 h after root pruning under well-watered conditions. The foregoing responses may be explained by the upregulation of plasma membrane intrinsic protein gene and increases in abscisic acid and jasmonic acid in roots. Increased auxin and salicylic acid contributed to the compensated lateral root growth. In conclusion, root pruning improved WUE in maize by root water uptake.

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The formation of Casparian strips (CS) and the deposition of suberin at the endodermis of plant roots are thought to limit the apoplastic transport of water and ions. We investigated the specific role of each of these apoplastic barriers in the control of hydro-mineral transport by roots and the consequences on shoot growth. A collection of Arabidopsis thaliana mutants defective in suberin deposition and/or CS development was characterized under standard conditions using a hydroponic system and the Phenopsis platform. Mutants altered in suberin deposition had enhanced root hydraulic conductivity, indicating a restrictive role for this compound in water transport. In contrast, defective CS directly increased solute leakage and indirectly reduced root hydraulic conductivity. Defective CS also led to a reduction in rosette growth, which was partly dependent on the hydro-mineral status of the plant. Ectopic suberin was shown to partially compensate for defective CS phenotypes. Altogether, our work shows that the functionality of the root apoplastic diffusion barriers greatly influences the plant physiology, and that their integrity is tightly surveyed.

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Soil salinity reduces root hydraulic conductivity (Lpr) of several plant species. However, how cellular signaling and root hydraulic properties are linked in plants that can cope with water restriction remains unclear. In this work, we exposed the halotolerant species red beet (Beta vulgaris) to increasing concentrations of NaCl to determine the components that might be critical to sustaining the capacity to adjust root hydraulics. Our strategy was to use both hydraulic and cellular approaches in hydroponically grown seedlings during the first osmotic phase of salt stress. Interestingly, Lpr presented a bimodal profile response apart from the magnitude of the imposed salt stress. As well as Lpr, the PIP2-aquaporin profile follows an unphosphorylated/phosphorylated pattern when increasing NaCl concentration while PIP1 aquaporins remain constant. Lpr also shows high sensitivity to cycloheximide. In low NaCl concentrations, Lpr was high and 70 % of its capacity could be attributed to the CHX-inhibited cell-to-cell pathway. More interestingly, roots can maintain a constant spontaneous exudated flow that is independent of the applied NaCl concentration. In conclusion, Beta vulgaris root hydraulic adjustment completely lies in a dominant cell-to-cell pathway that contributes to satisfying plant water demands.

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Zinc (Zn) is involved in plant growth and stress resistance and is known to increase crop yield. Here, we investigated the effect of Zn on water absorption in the roots of maize (Zea mays L.), a crop which is sensitive to Zn deficiency, during water stress conditions. Seedlings of the maize variety "Zhengdan 958" were cultivated with 0.1 or 6 µM ZnSO4·7H2O. To simulate drought stress, three-week-old seedlings were exposed to 15% polyethylene glycol (PEG). Root growth parameters, root antioxidant enzyme activity, root hydraulic conductivity, root aquaporin gene expression, root and leaf anatomy structure, leaf water potential, chlorophyll content, leaf area, and gas exchange parameters were measured. Under water stress, moderate Zn treatment promoted root growth; maintained root and leaf anatomy structural integrity. Moderate Zn significantly increased roots hydraulic conductivity (51%) and decreased roots antioxidant enzyme activity (POD: -11.1%, CAT: -35.1%, SOD: -3.1%) compared with low-level Zn under water stress. The expression of ZmPIP1;1, ZmPIP1;2, and ZmPIP2;2 was significantly higher with moderate Zn treatment than that of low-level Zn treatment. The leaf water potential, chlorophyll content, leaf area, and gas exchange parameters with moderate Zn treatment increased significantly under water stress compared with low-level Zn treatment. The moderate concentration of Zn improved root hydraulic conductivity in maize and increased resistance to simulated drought conditions by maintaining root structural integrity, decreasing antioxidant enzyme activity, and increasing aquaporin gene expression. Moderate Zn application increased root water absorption and leaf transpiration, thereby maintaining maize water balance under water stress conditions.

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Environments with short growing seasons and variable climates can have soil temperatures that are suboptimal for chilling-sensitive crops. These conditions can adversely affect root growth and physiological performance thus impairing water and nutrient uptake. Four greenhouse trials and a field study were conducted to investigate if rootstocks can enhance tomato performance under suboptimal soil temperatures (SST). In a controlled greenhouse environment, we exposed four commercial rootstocks (Estamino, Maxifort, RST-04-106-T, and Supernatural) grafted with a common scion (cv. BHN-589) to optimal (mean: 24°C) and SST (mean: 13.5°C) and compared their performance with the non-grafted BHN-589 cultivar. Several root and shoot physiological traits were evaluated: root hydraulic conductivity and conductance, root anatomy, leaf gas exchange, leaf δ13C, shoot C and N, and biomass. Under field conditions, the same five phenotypes were evaluated for canopy growth, normalized difference vegetation index (NDVI), leaf nutrients, biomass, and yield. Under SST, root hydraulic conductivity (Lp) and conductance (K R), stomatal conductance (g s), and plant biomass decreased. Hydrostatic Lp decreased more than osmotic Lp (Lp ∗ hyd: 39-65%; Lp ∗ os: 14-40%) and some of the reduced conductivity was explained by the increased cortex area of primary roots observed under SST (67-140%). Under optimal soil temperatures, all rootstocks conferred higher g s than the non-grafted cultivar, but only two rootstocks maintained higher g s under SST. All phenotypes showed greater reductions in shoot biomass than root biomass resulting in greater (∼20%) root-to-shoot ratios. In the field, most grafted phenotypes increased early canopy cover, NDVI, shoot biomass, and fruit yield. Greenhouse results showed that Lp ∗ os may be less affected by SST than Lp ∗ hyd and that reductions in Lp may be offset by enhanced root-to-shoot ratios. We show that some commercial rootstocks possess traits that maintained better rates of stomatal conductance and shoot N content, which can contribute toward better plant establishment and improved performance under SST.

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In order to improve the understanding of plant water relations under drought stress, the water use behavior of two Fragaria x ananassa Duch. cultivars, contrasting in their drought stress phenotype, is identified. Under drought, stomatal closure is gradual in Figaro. Based on this, we associate Figaro with conservative water use behavior. Contrarily, drought stress causes a sudden and steep decrease in stomatal conductance in Flair, leading to the identification of Flair as a prodigal water use behavior cultivar. Responses to progressive drought on the one hand and an osmotic shock on the other hand are compared between these two cultivars. Tonoplast intrinsic protein mRNA levels are shown to be upregulated under progressive drought in the roots of Figaro only. Otherwise, aquaporin expression upon drought or osmotic stress is similar between both cultivars, i.e. plasma membrane intrinsic proteins are downregulated under progressive drought in leaves and under short term osmotic shock in roots. In response to osmotic shock, root hydraulic conductivity did not change significantly and stomatal closure is equal in both cultivars. De novo abscisic acid biosynthesis is upregulated in the roots of both cultivars under progressive drought.

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Eutrema salsugineum is considered as extremophile model species. To gain insights into the root hydraulic conductivity and the role played by aquaporins in E. salsugineum, we investigated the aquaporin family profiles, plant water status and root hydraulic conductivity under standard (salt-free) and salt stress conditions. We found that there was no variation in the relative electric conductivity of the leaves when the salt concentration was less than 200 mM NaCl, and the transpiration rate dropped to 60.6% at 100 mM NaCl for 14 days compared to that at standard conditions. The pressure chamber techniques indicated that the root hydraulic conductivity of E. salsugineum was repressed by salt stress. However, propionic acid, usually used as an aquaporin inhibitor, unexpectedly enhanced the root hydraulic conductivity of E. salsugineum. The aquaporin family in E. salsugineum was profiled and the PIP aquaporin expression was investigated at the transcriptional and translational levels. Finally, two EsPIPs were identified to play a role in salt stress. The overall study provides evidence on how halophytes maintain their water status and aquaporin regulation pattern under salt stress conditions.

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BACKGROUND: Mineral nutrient limitation affects the water flow through plants. We wanted to test on barley whether any change in root-to-shoot ratio in response to low supply of nitrogen and phosphate is accompanied by changes in root and cell hydraulic properties and involves changes in aquaporin (AQP) gene expression and root apoplastic barriers (suberin lamellae, Casparian bands). METHODS: Plants were grown hydroponically on complete nutrient solution or on solution containing only 3.3 % or 2.5 % of the control level of nutrient. Plants were analysed when they were 14-18 d old. RESULTS: Nutrient-limited plants adjusted water flow to an increased root-to-shoot surface area ratio through a reduction in root hydraulic conductivity (Lp) as determined through exudation analyses. Cortex cell Lp (cell pressure probe analyses) decreased in the immature but not the mature region of the main axis of seminal roots and in primary lateral roots. The aquaporin inhibitor HgCl2 reduced root Lp most in nutrient-sufficient control plants. Exchange of low-nutrient for control media caused a rapid (20-80 min) and partial recovery in Lp, though cortex cell Lp did not increase in any of the root regions analysed. The gene expression level (qPCR analyses) of five plasma membrane-localized AQP isoforms did not change in bulk root extracts, while the formation of apoplastic barriers increased considerably along the main axis of root and lateral roots in low-nutrient treatments. CONCLUSIONS: Decrease in root and cortex cell Lp enables the adjustment of root water uptake to increased root-to-shoot area ratio in nutrient-limited plants. Aquaporins are the prime candidate to play a key role in this response. Modelling of water flow suggests that some of the reduction in root Lp is due to increased formation of apoplastic barriers.

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With large forested urban areas, the city of Edmonton, Alberta, Canada, faces high annual costs of replacing trees injured by deicing salts that are commonly used for winter road maintenance. Ectomycorrhizal fungi form symbiotic associations with tree roots that allow trees to tolerate the detrimental effects of polluted soils. Here, we examined mycorrhizal colonization of Pinus contorta by germinating seeds in soils collected from different locations: (1) two urban areas within the city of Edmonton, and (2) an intact pine forest just outside Edmonton. We then tested the responses of seedlings to 0-, 60-, and 90-mM NaCl. Our results showed lower abundance and diversity of ectomycorrhizal fungi in seedlings colonized with the urban soils compared to those from the pine forest soil. However, when subsequently exposed to NaCl treatments, only seedlings inoculated with one of the urban soils containing fungi from the genera Tuber, Suillus, and Wilcoxina, showed reduced shoot Na accumulation and higher growth rates. Our results indicate that local ectomycorrhizal fungi that are adapted to challenging urban sites may offer a potential suitable source for inoculum for conifer trees designated for plating in polluted urban environments.

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Current theory and supporting research suggests that radial transport is the most limiting factor to root water uptake, raising the question whether only absorbing root length and radial conductivity matter to water uptake. Here, we extended the porous pipe analytical model of root water uptake to entire root networks in 3D and analysed the relative importance of axial and radial characteristics to total uptake over parameter ranges reported in the literature. We found that network conductance can be more sensitive to axial than radial conductance of absorbing roots. When axial transport limits uptake, more dichotomous topology, especially towards the base of the network, increases water uptake efficiency, while the effect of root length is reduced. Whole root system conductance was sensitive to radial transport and length in model lupin (Lupinus angustifolius L.), but to axial transport and topology in wheat (Triticum aestivum L.), suggesting the root habit niche space of monocots may be constrained by their loss of secondary growth. A deep tap root calibrated to oak (Quercus fusiformis J. Buchholz) hydraulic parameters required 15 times more xylem volume to transport comparable amounts of water once recalibrated to parameters from juniper (Juniperus ashei Small 1901), showing that anatomical constraints on axial conductance can lead to significant trade-offs in woody roots as well. Root system water uptake responds to axial transport and can be limited by it in a biologically meaningful way.

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The argane tree is a remarkable essence by its botanical interest and its socioeconomic value. It is endemic species in the southwest of Morocco, where prolonged drought stress may occur. Although its tolerance has been commonly attributed to various mechanisms at the whole plant, the root system has a main role in the whole process of adaptation. We studied in argane tree plants the change in hydraulic conductivity, electrolyte leakage in root as well as root growth under drought stress and recovery. Our findings showed that the root hydraulic conductivity (Lpr) value significantly decreased under drought stress treatment. This was associated with an increase of root electrolyte leakage, signaling the occurrence of an injury to root cell membranes. At root growth level, stressed plants managed to maintain their root elongation despite decreased root mass. After short period of rehydration, the argane tree plants exhibited a tendency of increased hydraulic conductivity during recovery after drought stress, suggesting that this root physiological response may be intimately linked to drought stress tolerance strategies. These results also could be important to contribute to selection of tolerant genotypes and develop argane tree regeneration programs in regions that suffer from lack of water.

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End-of-season drought or "terminal drought," which occurs after flowering, is considered the most significant abiotic stress affecting crop yields. Wheat crop production in Mediterranean-type environments is often exposed to terminal drought due to decreasing rainfall and rapid increases in temperature and evapotranspiration during spring when wheat crops enter the reproductive stage. Under such conditions, every millimeter of extra soil water extracted by the roots benefits grain filling and yield and improves water use efficiency (WUE). When terminal drought develops, soil dries from the top, exposing the top part of the root system to dry soil while the bottom part is in contact with available soil water. Plant roots sense the drying soil and produce signals, which on transmission to shoots trigger stomatal closure to regulate crop water use through transpiration. However, transpiration is linked to crop growth and productivity and limiting transpiration may reduce potential yield. While an early and high degree of stomatal closure affects photosynthesis and hence biomass production, a late and low degree of stomatal closure exhausts available soil water rapidly which results in yield losses through a reduction in post-anthesis water use. The plant hormone abscisic acid (ABA) is considered the major chemical signal involved in stomatal regulation. Wheat genotypes differ in their ability to produce ABA under drought and also in their stomatal sensitivity to ABA. In this viewpoint article we discuss the possibilities of exploiting genotypic differences in ABA response to soil drying in regulating the use of water under terminal drought. Root density distribution in the upper drying layers of the soil profile is identified as a candidate trait that can affect ABA accumulation and subsequent stomatal closure. We also examine whether leaf ABA can be designated as a surrogate characteristic for improved WUE in wheat to sustain grain yield under terminal drought. Ease of collecting leaf samples to quantify ABA compared to extracting xylem sap will facilitate rapid screening of a large number of germplasm for drought tolerance.

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MAIN CONCLUSION: The effect of ethylene and its precursor ACC on root hydraulic properties, including aquaporin expression and abundance, is modulated by relative air humidity and plant sensitivity to ethylene. Relative air humidity (RH) is a main factor contributing to water balance in plants. Ethylene (ET) is known to be involved in the regulation of root water uptake and stomatal opening although its role on plant water balance under different RH is not very well understood. We studied, at the physiological, hormonal and molecular levels (aquaporins expression, abundance and phosphorylation state), the plant responses to exogenous 1-aminocyclopropane-1-carboxylic acid (ACC; precursor of ET) and 2-aminoisobutyric acid (AIB; inhibitor of ET biosynthesis), after 24 h of application to the roots of tomato wild type (WT) plants and its ET-insensitive never ripe (nr) mutant, at two RH levels: regular (50%) and close to saturation RH. Highest RH induced an increase of root hydraulic conductivity (Lpo) of non-treated WT plants, and the opposite effect in nr mutants. The treatment with ACC reduced Lpo in WT plants at low RH and in nr plants at high RH. The application of AIB increased Lpo only in nr plants at high RH. In untreated plants, the RH treatment changed the abundance and phosphorylation of aquaporins that affected differently both genotypes according to their ET sensitivity. We show that RH is critical in regulating root hydraulic properties, and that Lpo is affected by the plant sensitivity to ET, and possibly to ACC, by regulating aquaporins expression and their phosphorylation status. These results incorporate the relationship between RH and ET in the response of Lpo to environmental changes.

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