RESUMEN
This investigation was directed to examine the influence of copper oxide nanoparticles (CuO-NPs) on the hatchability traits, and chick quality of newly hatched broiler chicks. A total of 480 eggs were randomly divided into four treatment groups, each consisting of three duplicates. As a negative control (NC), the first group was not injected; the second group was injected with saline and served as a positive control (PC), the third and fourth groups were injected with 30 and 60 ppm of (CuO-NPs)/egg. Eggs were injected into the amniotic fluid on the eighteenth day of the incubation period. Results showed that the hatchability, chick yield %, yolk free-body mass (YFBM), chick length, shank length (SL), and relative weight of the heart, gizzard and intestine of day-old broiler chicks were all unaffected by the in ovo injection of CuO-NPs. The Pasgar Score was slightly improved compared to the NC and PC groups. Also, the in ovo administration of CuO-NPs (60 ppm/egg) significantly increased the intestine length. Both levels of CuO-NPs significantly increased the concentration of Cu ions in the hepatic tissue. Additionally, different levels of tissue damage were seen in the liver of the birds that were given low or high dosages of CuO-NPs. Conclusively, the in ovo injection of CuO-NPs has a good result on the appearance of the chicks (Pasgar score). However, negative effect of CuO-NPs on liver tissue may raise concerns about the potential risks of applying CuO-NPs in ovo administration.
RESUMEN
The effect of supplementing drinking water with sweet citrus peel powder (SCPP) on the performance, ileal microbial count, and relative weight of organs of broiler chickens reared in a tropical environment was investigated. Ninety-six (96) 1-day-old Ross broiler chickens, after brooding, were randomly allotted into four treatment groups: T1 (control) received drinking water without SCPP, while birds in T2, T3, and T4 were given water supplemented with 2, 4, and 6 g of SCPP per liter of water, respectively. Each treatment was replicated three times with eight birds per replicate in a completely randomized design. Feed intake and weight gain were not significantly (p > 0.05) different among the treatments. However, significant variations (p < 0.05) were observed in the final weight and feed conversion ratio (FCR) with birds on T4 (6 g SCPP) having the highest final weight of 2164.60 g and the lowest FCR of 2.11 compared with 1838.40 g final weight and 2.40 FCR recorded for birds in the control group. Total bacteria count (TBC) of 2.07 × 106 CFU/ml (T1), 1.20 × 106 CFU/ml (T2), 1.27 × 106 CFU/ml (T3), and 1.33 × 106 CFU/ml (T4) recorded showed no significant (p > 0.05) variations among the treatments. However, orthogonal contrast between control and SCPP treatment groups showed significant variation (p < 0.05) in TBC. Significant variations (p < 0.05) were observed in the live weight and relative weight of heart and pancreas. Higher live weight with lower FCR and TBC recorded in this study showed the positive effect of SCPP on the performance and ileal microbial count of broiler chickens.