RESUMEN
Muhlenbergia spatha, previously known only from near the type locality in San Luis Potosí, is reported from two localities in Zacatecas, Mexico. Historically, botanists have overlooked this diminutive annual. To clarify affinities of M. spatha, we present a molecular phylogeny emphasising species in M. subg. Pseudosporobolus using sequence data from two plastid markers (rpl32-trnL and rps16 intron) and nrDNA ITS. In addition, we include an updated description, illustration and discussion of the habitat of M. spatha.
Resumen Muhlenbergia spatha, anteriormente conocida solo cerca de la localidad tipo en San Luis Potosí, se reporta en dos localidades en Zacatecas, México. Históricamente, los botánicos han pasado por alto esta diminuta anual. Para aclarar las afinidades de M. spatha, presentamos una filogenia molecular que enfatiza especies en M. subg. Pseudosporobolus usando datos de secuencia de dos marcadores plástidos (rpl32-trnL e rps16 intron) y nrADN EIT. Además, incluimos una descripción actualizada, ilustración y discusión del hábitat de M. spatha.
RESUMEN
A taxonomic treatment, phylogeny based on analysis of six DNA sequence markers (ITS, ndhA intron, rpl32-trnL, rps3, rps16 intron and rps16-trnK) and classification of Muhlenbergia for Peru is given. Seventeen species and one presumed hybrid are recognised. Muhlenbergiaromaschenkoi sp. nov. is newly described from the Río Huallaga Valley, northeast of Huánuco. The type of Podosemumangustatum [≡ Muhlenbergiaangustata] clearly aligns with what we had been referring to as the hybrid between this species and M.rigida. Therefore, we adopt the next available heterotypic name, Muhlenbergiacoerulea, for what we had been calling M.angustata and change the hybrid designation to M.coerulea × M.rigida. Lectotypes are designated for Epicampescoerulea Griseb., Muhlenbergiaaffinis Trin., Muhlenbergiaberlandieri Trin., Muhlenbergiabeyrichiana Kunth, Muhlenbergiaelegansvar.atroviolacea Kuntze, Muhlenbergiaelegansvar.subviridis Kuntze and Muhlenbergiaphragmitoides Griseb.
ResumenBrindamos un tratamiento taxonómico, una filogenia basado en el análisis de seis marcadores de secuencia de ADN (ITS, intrón ndhA, rpl32-trnL, rps3, intrón rps16, rps16-trnK) y la clasificación de Muhlenbergia para Perú. Se reconocen diecisiete especies y un supuesto híbrido. Muhlenbergiaromaschenkoi sp. nov. es descrita como especie nueva procedente del valle de Huallaga, al noreste de Huánuco. El tipo de Podosemumangustatum [≡ Muhlenbergiaangustata] se alinea claramente con lo que nosotros habíamos referido como el híbrido entre esta especie y M.rigida. Por lo tanto, adoptamos el siguiente nombre heterotípico disponible, Muhlenbergiacoerulea para lo que habíamos estado llamando M.angustata, y cambiamos la designación híbrida a M.coerulea × M.rigida. Los lectotipos son designados para Epicampescoerulea Griseb., Muhlenbergiaaffinis Trin., Muhlenbergiaberlandieri Trin., Muhlenbergiabeyrichiana Kunth, Muhlenbergiaelegansvar.atroviolacea Kuntze, Muhlenbergiaelegansvar.subviridis Kuntze y Muhlenbergiaphragmitoides Griseb.
RESUMEN
Mountain uplifts have generated new ecologic opportunities for plants, and triggered evolutionary processes, favouring an increase on the speciation rate in all continents. Moreover, mountain ranges may act as corridors or barriers for plant lineages and populations. In South America a high rate of diversification has been linked to Andean orogeny during Pliocene/Miocene. More recently, Pleistocene glacial cycles have also shaped species distribution and demography. The endemic genus Escallonia is known to have diversified in the Andes. Species with similar morphology obscure species delimitation and plants with intermediate characters occur naturally. The aim of this study is to characterize genetic variation and structure of two widespread species of Escallonia: E. alpina and E. rubra We analyzed the genetic variation of populations of the entire distribution range of the species and we also included those with intermediate morphological characters; a total of 94 accessions from 14 populations were used for the Amplified Fragment Length Polymorphism (AFLP) analysis. Plastid DNA sequences (trnS-trnG, 3'trnV-ndhC intergenic spacers and the ndhF gene) from sixteen accessions of Escallonia species were used to construct a Statistical Parsimony network. Additionally, we performed a geometric morphometrics analysis on 88 leaves from 35 individuals of the two E. alpina varieties to further study their differences. Wright's Fst and analysis of molecular variance tests performed on AFLP data showed a significant level of genetic structure at the species and population levels. Intermediate morphology populations showed a mixed genetic contribution from E. alpina var. alpina and E. rubra both in the Principal Coordinates Analysis (PCoA) and STRUCTURE. On the other hand, E. rubra and the two varieties of E. alpina are well differentiated and assigned to different genetic clusters. Moreover, the Statistical Parsimony network showed a high degree of divergence between the varieties of E. alpina: var. alpina is more closely related to E. rubra and other species than to its own counterpart E. alpina var. carmelitana Geometric morphometrics analysis (Elliptic Fourier descriptors) revealed significant differences in leaf shape between varieties. We found that diversity in Escallonia species analyzed here is geographically structured and deep divergence between varieties of E. alpina could be associated to ancient evolutionary events like orogeny. Admixture in southern populations could be the result of hybridization at the margins of the parental species' distribution range.