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The application of ureolytic bacteria for bioremediation of soil contaminated with heavy metals, including cadmium (Cd), allows for the efficient immobilization of heavy metals by precipitation or coprecipitation with carbonates. Microbially-induced carbonate precipitation process may be useful also in the case of the cultivation of crop plants in various agricultural soils with trace but legally permissible Cd concentrations, which may be still uptaken by plants. This study aimed to investigate the influence of soil supplementation with metabolites containing carbonates (MCC) produced by the ureolytic bacterium Ochrobactrum sp. POC9 on the Cd mobility in the soil as well as on the Cd uptake efficiency and general condition of crop plants (Petroselinum crispum). In the frame of the conducted studies (i) carbonate productivity of the POC9 strain, (ii) the efficiency of Cd immobilization in soil supplemented with MCC, (iii) crystallization of cadmium carbonate in the soil enriched with MCC, (iv) the effect of MCC on the physico-chemical and microbiological properties of soil, and (v) the effect of changes in soil properties on the morphology, growth rate, and Cd-uptake efficiency of crop plants were investigated. The experiments were conducted in soil contaminated with a low concentration of Cd to simulate the natural environmental conditions. Soil supplementation with MCC significantly reduced the bioavailability of Cd in soil with regard to control variants by about 27-65% (depending on the volume of MCC) and reduced the Cd uptake by plants by about 86% and 74% in shoots and roots, respectively. Furthermore, due to the decrease in soil toxicity and improvement of soil nutrition with other metabolites produced during the urea degradation (MCC), some microbiological properties of soil (quantity and activity of soil microorganisms), as well as the general condition of plants, were also significantly improved. Soil supplementation with MCC enabled efficient Cd stabilization and significantly reduced its toxicity for soil microbiota and plants. Thus, MCC produced by POC9 strain may be used not only as an effective Cd immobilizer in soil but also as a microbe and plant stimulators.

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Numerous reports of graphene-family nanomaterials (GFNs) promoting plant growth have opened up a wide range of promising potential applications in agroforestry. However, several toxicity studies have raised growing concerns about the biosafety of GFNs. Although these studies have provided clues about the role of GFNs from different perspectives (such as plant physiology, biochemistry, cytology, and molecular biology), the mechanisms by which GFNs affect plant growth remain poorly understood. In particular, a systematic collection of data regarding differentially expressed genes in response to GFN treatment has not been conducted. We summarize here the fate and biological effects of GFNs in plants. We propose that soil environments may be conducive to the positive effects of GFNs but may be detrimental to the absorption of GFNs. Alterations in plant physiology, biochemistry, cytological structure, and gene expression in response to GFN treatment are discussed. Coincidentally, many changes from the morphological to biochemical scales, which are caused by GFNs treatment, such as affecting root growth, disrupting cell membrane structure, and altering antioxidant systems and hormone concentrations, can all be mapped to gene expression level. This review provides a comprehensive understanding of the effects of GFNs on plant growth to promote their safe and efficient use.

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Epichloë endophytes are biotrophic fungi that establish mutualistic symbiotic relationship with grasses and affect performance of the host under different environments. Wild barley (Hordeum brevisubulatum) is an important forage grass and often infected by Epichloë bromicola, thus showing tolerances to stresses. Since the plant growth correlates with both microbial infection and nutrient stoichiometry, this study was performed to investigate whether the function of Epichloë bromicola endophyte to improve host growth depend upon the nitrogen (N), phosphorus (P) fertilization. Epichloë-infected (E+) and Epichloë-free (E-) wild barley plants were subjected to nine types of mixed N (0.2 mM, 3 mM, 15 mM) and P (0.01 mM, 0.1 mM, 1.5 mM) levels treatments for 90 d to collect plant samples and determine multiple related indexes. We found that E. bromicola and N, P additions positively affected seed germination. Further, E. bromicola significantly enhanced chlorophyll content and root metabolic activity under N-deficiency, and meanwhile, might alter allocation of photosynthate under different conditions. The contents of N, P and stoichiometry of C:N:P of E+ plants were significantly higher than that of E- under nutrient deficiency, but contrary results were observed under adequate nutrients. Therefore, we propose that the growth-promoting ability of E. bromicola is closely correlated with N and P additional levels. Under low N, P additions, positive roles of endophyte are significant as opposed to negative roles under high N, P additions.

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Perennial ryegrass (Lolium perenne) is widely cultivated around the world for turf and forage. However, the plant is highly susceptible to disease and is sensitive to drought. The present study aims to determine the effect of the fungal endophyte Epichloë festucae var. lolii of perennial ryegrass on the combined stresses of drought and disease caused by Bipolaris sorokiniana in the greenhouse. In the experiment, plants infected (E+) or not infected (E-) with the fungal endophyte were inoculated with Bipolaris sorokiniana and put under different soil water regimes (30%, 50%, and 70%). The control treatment consisted of E+ and E- plants not inoculated with B. sorokiniana. Plant growth, phosphorus (P) uptake, photosynthetic parameters, and other physiological indices were evaluated two weeks after pathogen infection. The fungal endophyte in E+ plants increased P uptake, plant growth, and photosynthetic parameters but decreased the malondialdehyde concentration, proline content, and disease incidence of perennial ryegrass (p < 0.05). E+ plants had the lowest disease incidence at 70% soil water (p < 0.05). The study demonstrates that the fungal endophyte E. festucae var. lolii is beneficial for plant growth and stress tolerance in perennial ryegrass exposed to the combined stresses of drought and B. sorokiniana.

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The aim of this study is to evaluate the effects of biochar on the plant's growth. A pot experiment was carried out in our study. Rice straw-derived biochar were charred at two heating temperatures (400 °C/800 °C) and two oxygen-limited atmospheres (CO2/N2), respectively. The FESEM/EDS technique (field emission scanning electron microscopy with X-ray energy-dispersive spectroscopy) was used to study soils, biochar and plant samples. FESEM images indicated that the structure of the biochar was highly heterogeneous with larger macropores, which can enhance soil porosity. Fine soil mineral particles blocked the biochar inner pores and channels after returning biochar to soil. EDS analysis indicated that the Al and Fe contents increased on the surface of biochar after their returning, which reduced the toxicity of Al and Fe in the soil. The returning straw directly inhibited the growth of leaf-used lettuce. Four returning biochar all significantly improved leaf-used lettuce growth, and the effects of biochar prepared under 400 °C and a CO2 atmosphere were better than those prepared under 800 °C and a N2 atmosphere. Changes of nitrogen content in the biochar before and after their returning were consistent with the improvement of soil available nitrogen, and plant growth was positively correlated with the nitrogen content of biochar. This study explored the impact of biochar on soil nutrients and revealed the mechanism of biochar returning to the field to promote plant growth. It is of great significance in studying and improving the characteristics of soil nutrients.

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In this paper, we present a novel method for vision based plants phenotyping in indoor vertical farming under artificial lighting. The method combines 3D plants modeling and deep segmentation of the higher leaves, during a period of 25-30 days, related to their growth. The novelty of our approach is in providing 3D reconstruction, leaf segmentation, geometric surface modeling, and deep network estimation for weight prediction to effectively measure plant growth, under three relevant phenotype features: height, weight and leaf area. Together with the vision based measurements, to verify the soundness of our proposed method, we also harvested the plants at specific time periods to take manual measurements, collecting a great amount of data. In particular, we manually collected 2592 data points related to the plant phenotype and 1728 images of the plants. This allowed us to show with a good number of experiments that the vision based methods ensure a quite accurate prediction of the considered features, providing a way to predict plant behavior, under specific conditions, without any need to resort to human measurements.

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The challenge of environment management is a sustainable development of both environmental systems and the green economy. The main objective of this study was the optimization and testing of plant growth-promoting rhizobacteria soil biopreparation and soil amendments as the alternative to or to help offset use of mineral soil fertilizers. A field study was conducted on a degraded area to improve plant biomass yield using inoculated bacteria. Moreover, organic additives (sewage sludge) and inorganic fertilizers were also used to compare their effectiveness. The conducted study investigated the combined effect of immobilized microbial consortium and soil substrates for plant growth (red fescue) on degraded areas and confirmed synergistic interactions between plants, microorganisms and soil substrates in the process of phytoremediation. The formulation consisted of immobilized bacterial consortia having mechanisms for the synthesis of compounds preferably affecting growth and development of (crop) plants. The conducted studies confirmed that the PGPR bacteria used in the experiment have the potential to promote plant growth, increase organic nitrogen the bioavailable P pool in soil. The results of the field study (synergy effect of alternative fertilization and bacteria) can be used to create effective methods in assisted soil bioremediation. This approach has a high social acceptance and reduces resource consumption of fertilizers having an impact on sustainable development. Implementing methods of supporting plant growth using microorganisms can undoubtedly contribute to the development of 'green' resource management.

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The outcome of competition between adjoining interspecific colonies of Phragmites and Typha in two large field pilot-scale free water surface (FWS) and subsurface flow (SSF) CWs is evaluated. According to findings, the effect of interspecific competition was notable for Phragmites australis, whereby it showed the highest growth performance in both FWS and SSF wetland. In a mixed-culture, P. australis demonstrates superiority in terms of competitive interactions for space between plants. Furthermore, the interspecific competition among planted species seemed to cause different ecological responses of plant species in the two CWs. For example, while relatively high density and shoot height determined the high aboveground dry weight of P. australis in the FWS wetland, this association was not evident in the SSF. Additionally, while plants nutrients uptake accounts for a higher proportion of the nitrogen removal in FWS, that in the SSF accounts for a higher proportion of the phosphorous removal.

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A produção de mudas de alta qualidade torna-se uma estratégia importante para o sucesso de uma cultura. Desta forma, o presente trabalho teve como objetivo avaliar o efeito de diferentes doses de bioestimulante Stimulate na formação de mudas de maracujazeiro. O experimento foi conduzido em casa de vegetação, em delineamento experimental em blocos casualizados, com parcelas subdivididas no tempo, com seis tratamentos e quatro repetições, sendo cada parcela composta de 24 plantas. Os tratamentos foram constituídos de diferentes doses de bioestimulante: (água destilada), 4, 12, 16, e 20 mL kg-1 sementes. Foram coletadas duas plantas por parcela aos 15, 22, 29, 36, 43 e 50 dias após a emergência. As características avaliadas foram altura de plantas, área foliar, massa seca de folhas, parte aérea, raízes, massa seca total, razão raiz/parte aérea, taxa de crescimento absoluto e relativo, taxa assimilatória líquida, razão de área foliar e área foliar específica. Verificou-se efeito positivo do bioestimulante sobre a maioria das variáveis avaliadas, em relação à testemunha. Doses entre 4 e 6 mL de Stimulate kg-1 de sementes promoveram maior equilíbrio entre o sistema radicular e a parte aérea, o que pode favorecer o estabelecimento da muda no campo

The production of seedlings of high quality becomes an important strategy for the success of a crop.The present research had the objective of evaluating the effect of different bioestimulant doses,Stimulate®, in the formation of passion fruit seedlings. The experiment was carried out in greenhouseconditions, in randomize complete blocks experimental design with six treatments and four replications,with 24 plants per plots. The treatments were constituted of different bioestimulante doses: 0 (distilledwater), 4, 12, 16 and 20 mL kg-1 seeds. Plants were collected 15, 22, 29, 36, 43 and 50 days after theemergency. Evaluations were plant height, leaf area, dries mass of leaves, shoot, root and total dry mass;root/shoot relation, absolute and relative growth rate, net assimilate rate, leaf area ratio and specific leafarea. Positive effect of the bioestimulant was verified on most of the appraised variables, in relation tothe control. Doses between 4 and 6 mL of Stimulate® kg-1 of seeds promoted better balance between theroot and shoot, which can improve the establishment of the seedlings in the field

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