RESUMEN

The most widely used technique for the production of DNA aptamers/oligonucleotides is chemical synthesis. Despite its effectiveness, this technique cannot be performed "in house", making the user fully dependent on a supplier. In this work, we present a simplified method by which it is possible to enzymatically produce DNA aptamers "in house". This new method uses the rolling circle replication followed by a unique cleavage step using the SchI endonuclease. Potentially, any oligonucleotide can be produced by the enzymatic method proposed in this study. To illustrate, we present the production of three variations of the 31-TBA aptamer, a single stranded DNA which has anticoagulant action.

Asunto(s)

Aptámeros de Nucleótidos/biosíntesis , ADN de Cadena Simple/biosíntesis , Técnicas de Amplificación de Ácido Nucleico , Oligodesoxirribonucleótidos/biosíntesis , Anticoagulantes/síntesis química , Anticoagulantes/metabolismo , Aptámeros de Nucleótidos/genética , Secuencia de Bases , Enzimas de Restricción del ADN/metabolismo , ADN de Cadena Simple/genética , G-Cuádruplex , Humanos , Oligodesoxirribonucleótidos/genética