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ETHNOPHARMACOLOGICAL RELEVANCE: Angola has an extraordinary plant diversity and a great ethnobotanical potential. However, there is a general lack of information about the first botanical explorations in the country and their contribution to the knowledge of the medicinal flora. AIM OF THE STUDY: The main aim of this study was to unveil the ethnobotanical legacy of José Maria Antunes and Eugène Dekindt, priests of the first Catholic mission in Huíla (Angola) and shed light on their contribution to the knowledge of medicinal wild plants of the country, including information on the uses, plant parts used, and preparation methods documented in the late 19th century. The findings are discussed considering recent ethnobotanical studies to offer a more comprehensive understanding of the historical and traditional uses of plants in Angola over the last two centuries. MATERIALS AND METHODS: Based on the information available in manuscripts and on the study of botanical collections preserved in herbaria of Portugal and Angola, we extracted relevant information about the species used in traditional medicine by the rural population of Huíla, the health conditions treated, and the mode of preparation and application. RESULTS: Our results revealed that Antunes and Dekindt conducted the first ethnobotanical study in Huíla, and documented a large number of medicinal wild plants. From these, we report 191 medicinal species, including 25 endemic and four introduced species, belonging to 56 plant families and 146 genera. Fabaceae family presents the highest richness of medicinal plants (39 taxa), followed by Rubiaceae (13), Asteraceae (10), and Apocynaceae (9). The illnesses reported were classified into 15 different categories, with the highest number of species (49) corresponding to unspecific conditions, such as general pains, chills, and fever. Thirty-seven species were reported for respiratory diseases, 31 for musculoskeletal problems, and 30 for digestive issues. Leaves were the most used plant part for medicinal purposes (84 species). Infusion was the most frequently described preparation method (40 species), followed by maceration (24 species), and powdering (36 species). CONCLUSIONS: The legacy of Antunes and Dekindt's work improves our understanding of Angola's botanical richness and traditional uses of plant resources. Our findings highlight the presence of unique medicinal resources in Angola, especially among endemic species, which hold the potential to improve the quality of life of rural communities. Moreover, our research underscores the lack of knowledge of medicinal species, emphasizing the risk of losing valuable historical information.

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Arbuscular mycorrhizal fungi (AMF) have a broad distribution and establish symbiotic relationships with vascular plants in tropical regions. They play a crucial role in enhancing plant nutrient absorption, mitigating pathogenic infections, and boosting the resilience of host plants to abiotic stresses, including drought under specific conditions. Many natural forests in Ethiopia are being replaced by monospecific plantations. However, the impact of these actions on AMF is unknown and, despite their ecological functions, AMF communities in various forest systems have not been thoroughly investigated. In this study, we assessed soil AMF communities in natural and plantation forests by DNA metabarcoding of the ITS2 rDNA region and assessed the influence of climate and environmental variables on the AMF community. In total, 193 AMF operational taxonomic units (OTUs), comprising nine families and 15 genera, were recorded. Glomerales was the dominant order (67.9 % of AMF OTUs) and Septoglomus fuscum, Diversispora insculpta, and Funneliformis mosseae were the dominant species. AMF were more abundant in natural forests than in plantation forests and the composition of AMF communities differed significantly from those of plantation forest. In plantation forests, soil pH, organic carbon, total nitrogen, and available phosphorus significantly influenced the composition of AMF communities, whereas in natural forest, electrical conductivity, annual rainfall, and cumulative rainfall before sample collection were significantly correlated with AMF. SIMPER analysis identified the AMF responsible for composition variances among different forest types, with the Glomeraceae family being the most significant contributor, accounting for nearly 60 % of the dissimilarity. Our findings further our understanding of the ecological niche function and the role of AMF in Ethiopia's natural forest systems and highlight the importance of prioritizing the sustainable development of degraded natural forests rather than plantations to ensure the preservation of habitats conducive to maintaining various AMF communities when devising conservation and management strategies.

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Paper is one of the most significant inventions in human civilization, which considerably advanced global cultural development. Pulping is a key step in the conversion of fiber raw materials into paper. Since its inception, pulping has rapidly evolved, continually adapting to technological advancements. Researchers are constantly investigating various types of raw materials for pulping. In this review, some of the materials employed in pulping are outlined, and the fiber content, pulping method, as well as the strength of wood and non-wood crop straw as pulping raw materials are analyzed and discussed. In addition, this review explores the effects of different materials under various pulping conditions and assesses the future trends in raw material selection for pulping while considering the current global environmental pressures.

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One of the biggest challenges for designers and manufacturers of furniture is to reduce the thickness of conventional furniture materials such as particleboard (PB), medium-density fibreboard (MDF) and plywood. Designing furniture based on thin (less than 16 mm) and ultra-thin materials (less than 10 mm) is desirable for aesthetic reasons and because of the substantial material savings. However, the use of thin and ultra-thin materials reduces the strength of the furniture, especially the strength and deformation resistance of the joints. This study aimed to establish the possibilities for efficient furniture construction made of thin and ultra-thin materials using mitre joints. For this purpose, 14 types of L-type joints were tested: 12 glued and 2 detachable. The joints were made of eight wood-based panels and one non-wood panel. The bending moments and the stiffness coefficient under compression were determined. The obtained results show that the mitre joints made of laminated material with high-pressure laminate (HPL), 8 mm thick, MDF achieved the highest bending moment, and the highest stiffness coefficient was achieved by joints made of 10 mm thick compact HPL. Compact HPL joints were significantly affected by the type of adhesive used. Detachable joints had a relatively high bending strength but very low stiffness.

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With the alarming increase in dying trees and massive logging in the Czech forests due to bark beetle infestation, the collection of non-wood forest products, a beneficial recreational activity in the Czech Republic, is now being promoted as an alternative to wood provisioning services. This paper aims to present findings on the non-wood forest product preferences in the country as part of a baseline assessment for promoting the usage. This study relied on the 2019 national survey data of public preferences in collecting forest berries, mushrooms, honey, and medicinal herbs. K-means cluster analysis was employed to classify the respondents. A binary logistic regression with a conditional forward approach was employed to identify the potential predictors of the high preference for each non-wood forest product. Data from 1,050 online respondents were included, and two groups of respondents were clustered based on their preferences for the entire non-wood forest, i.e., higher and lower utilization. The regression analysis revealed that frequent forest visitors were the primary predictor of high utilization of all non-wood forest products (between 1.437 to 4.579 odd ratios), in addition to age, gender, and location of the forest property. By clustering the respondents based on the high and low preferences in utilizing non-wood forest products, the promotion of this service, from recreational to potential livelihood activities and economic benefits, can be better targeted, e.g., target customer, infrastructure development in the location with high preferences, scenarios based on the type of owners (municipal or private forest owners), which in accordance to the national forest policy and laws, and, at the same time, maintain the ecological stability.

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Greenhouse culture is a practical approach to obtain non-wood forest products from berry fruit at a higher efficacy than resource silviculture in natural understory. In this study, three-year old black chokeberry (Aronia melanocarpa (Michx.) Elliott 1821) seedlings were transplanted to a greenhouse where sunlight was complemented by red- (69.4% red, 30.2% green, 0.4% blue) and blue-color (15.3% red, 64.9% green, 19.8% blue) light-emitting diode (LED) illuminations. Half of the planting soils were amended by spent mushroom residue (SMR) (not amendment as the control) and half the seedlings were sprayed by chitosan oligosaccharide (CO) on leaves. All treatments can increase seedling height, but only blue light reinforces the basal diameter growth. Compared to sunlight, exposure to blue light can promote leaf nitrogen and phosphorus concentrations, superoxide dismutase activity, and fruit proanthocyanidin content. The combination with CO addition will further increase chlorophyl a content, acid phosphatase activity, and total phenolics in fruit. SMR amended can induce the steady state uptake of nutrients but failed to impact fruit quality. Overall, we recommend the combination of blue light LED illumination plus CO addition to culture black chokeberry for the purpose to gain natural bioactive compounds.

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Increased demand for wood affects its price and thus contributes to the growing interest in raw materials that can be used as a partial or total substitute for wood in the production of particleboard. One of the raw materials for the production of particleboard can be Cannabis sativa or, more precisely, hemp shives. In this work, 7 variants of panels with a density of 650 kg/m3 with 10 and 25% hemp shives substitution in different layers were produced. Particleboards containing hemp shives were characterized by lower density compared to conventional particleboards. The shares of hemp shives at the levels of 10% and 25% have a slight impact on the MOR and MOE; additional IB showed no statistically significant differences between the conventional particleboards and particleboards with a share of hemp shives. For particleboards with 25% hemp shives, a reduction in swelling was observed relative to particleboards made entirely of industrial wood particles.

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High-resolution forest mapping technology is a powerful data source to assess the production capacity of forests regarding wood and non-wood ecosystem services. The study shows how to evaluate the potential benefits from forest management treatments devoted to increase mushroom supply. The study was developed in Central Spain, over a forest with important cultural and economic values attached to mushrooms. Airborne laser scanning (ALS), mushroom production models and mathematical programming as spatial optimization method are used to sequence, spatially and temporally, silviculture-oriented actions to enlarge mushroom provisioning. We present a tactical forest planning solution to incentivize mushroom yield driven by clustered silvicultural treatments applied to fine-grained segments derived from ALS data, and along a 5-year plan while embedding temporal and spatial dependencies. Mushroom yield can increase up to 18 % from current conditions if all area is treated. Our model integrates constraints to optimize the selection of segments yielding the highest benefits in terms of mushroom yield and timber removals during the treatments. The temporal sequencing was successful, so the annual interventions are scheduled aligned in space and in time to ease the actionability and realism of model outputs. The assessment of production potential is an informative, spatially and temporally explicit exercise to inform decision-makers on investment opportunities to enhance the supply of non-wood ecosystem services, tested with mushroom in this study but extendable to more non-wood ecosystem services.

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The current trends in micro-/nanofibers offer a new and unmissable chance for the recovery of cellulose from non-woody crops. This work assesses a technically feasible approach for the production of micro- and nanofibrillated cellulose (MNFC) from jute, sisal and hemp, involving refining and enzymatic hydrolysis as pretreatments. Regarding the latter, only slight enhancements of nanofibrillation, transparency and specific surface area were recorded when increasing the dose of endoglucanases from 80 to 240 mg/kg. This supports the idea that highly ordered cellulose structures near the fiber wall are resistant to hydrolysis and hinder the diffusion of glucanases. Mechanical MNFC displayed the highest aspect ratio, up to 228 for hemp. Increasing the number of homogenization cycles increased the apparent viscosity in most cases, up to 0.14 Pa·s at 100 s-1 (1 wt.% consistency). A shear-thinning behavior, more marked for MNFC from jute and sisal, was evidenced in all cases. We conclude that, since both the raw material and the pretreatment play a major role, the unique characteristics of non-woody MNFC, either mechanical or enzymatically pretreated (low dose), make it worth considering for large-scale processes.

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Climate change is considered a major factor affecting honeybees' behavior and productivity with major consequences in both honey and agricultural production. Many research studies have expressed serious concerns about the mass losses of bee colonies and the role of bees as pollinators, while others have underlined important issues for the impact of the increase in temperature on honeybee abundance and honey yields. In the present work, we draw our attention to Marchalina hellenica, which is the most important honeydew-producing insect in Greece. A statistically significant forecasting model for the effect of cold periods in February on the life cycle of the insect is constructed, with the aid of the Cumulative Logit Model and the theory of runs. The forecasting model may help beekeepers plan the timely exploitation of honeydew secretions of pine trees, which will be beneficial for beekeepers, the rural economy, and forest protection. The new suggested model also indicates that, in view of the climate change scenarios seen in the literature, the life cycle of M. hellenica is expected to be drastically shorter.

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To develop a facile and low-cost nanofibrils process with excellent feedstock adaptability, high-yield lignocellulose nanofibrils (LCNF) are produced directly from wood and non-wood biomass using glycerol solvent via screw extrusion pretreatment. Different LCNFs are obtained from four classical raw materials (polar, pine, bamboo, and wheat straw) in this research, followed by comparing their morphological, thermochemical, and mechanical properties. More than 70 wt% of LCNF could be obtained from low-cost substrates except for LCNF from wheat straw with 62.3 wt% yield. Besides, the morphology property of wood LCNF exhibit more uniform distribution over that of non-wood LCNF due to narrower size distribution. Strikingly, despite of the slightly lower LCNF crystallinity various from 52.4% to 62.6% obtained from four substrates, all the LCNFs separated from wood and non-wood biomass exhibit high thermal stability (Tmax over 330 °C), which is higher than conventional nanocellulose, indicating that the crystal area could be well maintained during the pretreated process. Moreover, all the LCNF films show excellent tensile strength which is close to nanocellulose materials. Besides, the Young's modulus of wood-based LCNF films is higher than that of non-wood based LCNF films. Overall, LCNF with excellent performance could be achieved from low-cost biomass by our facile process, which provides a feasible route for industrial production of bio-based nanofilms.

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The Philaenus spumarius L. (Hemiptera Aphrophoridae) is a xylem-sap feeder vector that acquires Xylella fastidiosa subsp. pauca ST53 during feeding on infected plants. The bacterium is the plant pathogen responsible for olive quick decline syndrome that has decimated olive trees in Southern Italy. Damage originates mainly from the insect vector attitude that multiplies the pathogen potentialities propagating Xf in time and space. The principal action to manage insect-borne pathogens and to contain the disease spread consists in vector and transmission control. The analysis of an innovative and sustainable integrated pest management quantitative strategy that targets the vector and the infection by combining chemical and physical control means demonstrates that it is possible to stop the Xylella invasion. This review updates the available topics addressing vectors' identification, bionomics, infection management, and induced disease by Xylella invasion to discuss major available tools to mitigate the damage consequent to the disease.

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The main objective of this work was to evaluate pulp produced by kraft cooking for wood materials (WMT) (Bougainvillea spectabilis, Ficus altissima, and F. elastica) and non-wood materials (NWMT) (Sorghum bicolor and Zea mays stalks) and to study the fungal activity of handsheets treated with Melia azedarach heartwood extract (MAHE) solutions. Through the aforementioned analyses, the ideal cooking conditions were determined for each raw material based on the lignin percentage present. After cooking, pulp showed a decrease in the Kappa number produced from WMT, ranging from 16 to 17. This was in contrast with NWMT, which had Kappa numbers ranging from 31 to 35. A difference in the optical properties of the pulp produced from WMT was also observed (18 to 29%) compared with pulp produced from NWMT (32.66 to 35.35%). As for the evaluation of the mechanical properties, the tensile index of the pulp ranged from 30.5 to 40 N·m/g for WMT and from 44.33 to 47.43 N·m/g for NWMT; the tear index ranged from 1.66 to 2.55 mN·m2/g for WMT and from 4.75 to 5.87 mN·m2/g for NWMT; and the burst index ranged from 2.35 to 2.85 kPa·m2/g for WMT and from 3.92 to 4.76 kPa·m2/g for NWMT. Finally, the double fold number was 3 compared with that of pulp produced from pulp, which showed good values ranging from 36 to 55. In the SEM examination, sheets produced from treated handsheets with extract from MAHE showed no growth of Aspergillus fumigatus over paper discs manufactured from B. speclabilis pulp wood. Pulp paper produced from Z. mays and S. bicolor stalks was treated with 1% MAHE, while pulp paper from F. elastica was treated with 0.50% and 1% MAHE. With the addition of 0.5 or 1% MAHE, Fusarium culmorum showed no increase in growth over the paper manufactured from B. speclabilis, F. altissima, F. elastica and Zea mays pulps with visual inhibition zones found. There was almost no growth of S. solani in paper discs manufactured from pulps treated with 1% MAHE. This is probably due to the phytochemical compounds present in the extract. The HPLC analysis of MAHE identified p-hydroxybenzoic acid, caffeine, rutin, chlorogenic acid, benzoic acid, quinol, and quercetin as the main compounds, and these were present in concentrations of 3966.88, 1032.67, 834.13, 767.81, 660.64, 594.86, and 460.36 mg/Kg extract, respectively. Additionally, due to the importance of making paper from agricultural waste (stalks of S. bicolor and Z. mays), the development of sorghum and corn with high biomass is suggested.

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BACKGROUND: The use of plants in rituals is a little explored corner of biocultural diversity which has developed through time within a complex socio-ecological system. Indeed, rituals are complex interactions between humans and biodiversity shaped by history, culture, and ethnic belonging. Yet, in Western Ukraine, such rituals were forbidden for over 50 years (1939-1991). The current revival of rituals by rural inhabitants is an untapped reservoir of local ecological knowledge. The aim of the present study was to identify the ritual use of wild and cultivated plants in two regions of Western Ukraine, Bukovina and Roztochya, and to compare the findings with historical data. Moreover, we analyzed attitudes toward the ritual use of plants and interactions with the local environment. METHODS: We conducted 31 in-depth semi-structured interviews among Orthodox Hutsuls of Bukovina and 16 interviews among Greek Catholic rural inhabitants of Roztochya during summer 2018 focusing on the ritual uses of plants. RESULTS: We documented  28 plant taxa among Bukovinian Hutsuls and 58 plant taxa among inhabitants in Roztochya that were used in 7 religious festivals (of which two were celebrated differently in the two communities). Plants were mainly used in bouquets, but also for decorating churches and houses or in fruit baskets. In both communities, almost 25% of the interviewees could not name the plants they collected for bouquets, but rather referred to "just beautiful green herbs" one can get in meadows, forests, and gardens. Comparison with historical data shows a smaller number of taxa currently used (wild taxa have been lost), yet the persistence of 18 taxa used both now and a century ago. CONCLUSIONS: Contemporary practices concerning the use of plants in Christian rituals in Bukovina and Roztochya can be contextualized in the broader phenomenon of the revitalization of traditional environmental knowledge and practices that have characterized Europe over the past 30 years and in particular Eastern Europe after socialism. The current religious use of plants is to a certain extent the revitalization of historical rituals supported by various internal (knowledge from older generations) and external (church authorities and fashion in the region) drivers. Further research should address changes in regions with longer and more severe prohibition of religious practices and their revival.

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Cellulose nanocrystals (CNCs) have been recognized as one of the most promising nanofillers in modern science and technology owing to their outstanding characteristics of renewability, biodegradability, excellent mechanical strength, and liquid crystalline behavior. Interestingly, these properties are dependent on their genetic and also on the isolation process. Therefore, this research aimed to unveil how the biological variations of cellulose can influence on the physical properties of the extracted CNCs. A standard optimized extraction process was adopted to isolate the CNCs from different sources. Extracted CNCs were compared through characterization tools, including Fourier Transformation Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetry Analysis (TGA), Dynamic Light Scattering (DLS), Field Emission Scanning Electron Microscopy (FE-SEM), Atomic Force Microscopy (AFM), and Polarized Optical Microscopy (POM). Different self-assembly patterns were observed for different CNCs, owing to their biological variations. The resultant nanocrystals displayed variable morphologies such as spherical, rod, and needle shape. The hydrodynamic diameter, crystallinity index, decomposition temperature, liquid crystallinity, and storage modulus were varied. Nanocrystals isolated from non-wood feedstock have shown a higher degree of polymerization of 108.2 and a high Crystllinity Index (C·I.) of 55.1%. The rod-like morphology with the liquid crystalline pattern was obtained at 3 wt% concentration for SCNC.

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One way of satisfying increased market demand and simultaneously achieving a reduced environmental load in the industrial paper production is the use of fibrous agricultural residues. The aims of this study were i) to investigate the effect of alkaline - hydrodynamic cavitation (HC) pre-treatments on the delignification of Miscanthus × giganteus stalks (MGS) and ii) establishing the suitability of MGS as feedstock and their exploitation in pulp and paper manufacturing. It was demonstrated that the proposed treatment is an efficient delignification method for the non-wood fiber sources, such as miscanthus. A significant outcome of this work was the observation that HC treatment preserved the fibres lengths and surface quality of raw MGS, but at the same time increased the amount of kinked and curled fibers present in cavitated miscanthus fibers. The average miscanthus fiber length was found to be relatively short at 0.45 (±0.28) mm, while the slenderness ratio, the flexibility coefficient and Runkel ratio values were calculated to be 28.13, 38.16 and 1.62, respectively. The estimated physical properties of MGS pulp hand-sheets were 24.88 (±3.09) N m g-1 as the tensile index, 0.92 (±0.06) kPa m2 g-1 as the burst index and 4.0 (±0.37) mN m2 g-1 as the tear index. Overall the current work demonstrated effective use of hydrodynamic cavitation for improving the processing in pulp and paper manufacturing.

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Local cuisine is an important reservoir of local ecological knowledge shaped by a variety of socio-cultural, economic, and ecological factors. The aim was to document and compare the current use of wild and semi-cultivated plant food taxa by Romanians living in Romania and Ukraine. These two groups share similar ecological conditions and historically belonged to the same province, but were divided in the 1940s by the creation of a state border. We conducted 60 semi-structured interviews with rural residents. The contemporary use of 46 taxa (plus 5 cultivated taxa with uncommon uses), belonging to 20 families, for food consumption were recorded. Romanians in Romanian Bukovina used 27 taxa belonging to 15 families, while in Ukraine they used 40 taxa belonging to 18 families. Jams, sarmale, homemade beer, and the homemade alcoholic drink "socata" are used more by Romanians in Southern Bukovina, while tea, soups, and birch sap are used more in Northern Bukovina. We discuss the strong influence of socio-political scenarios on the use of wild food plants. Cross-ethnic marriages, as well as markets and women's networks, i.e., "neighbors do so", may have had a great impact on changes in wild food use. In addition, rapid changes in lifestyle (open work market and social migration) are other explanations for the abandonment of wild edible plants.

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The modern-day paper industry is highly capital-intensive industries in the core sector. Though there are several uses of paper for currency, packaging, education, information, communication, trade and hygiene, the flip side of this industry is the impact on the forest resources and other ecosystems which leads to increasing pollution in water and air, influencing several local communities. In the present paper, the authors have tried to explore potential and alternate source of industrial pulp through ruminant animal dung, which is widely available as a rural resource in India. Three types of undigested animal dung fibers from Indigenous cow (IDF), Jersey cow (JDF), and Buffalo (BDF) were taken. Wheat straw (WS) was the main diet of all animals. The cellulose, hemicellulose and lignin content for all animal dung samples were found in a range of (29-31.50%), (21-23.50%), and (11-13%), respectively. The abundant holocellulose and low lignin contents are suitable for handmade pulp and paper. Surface characteristics of fodder (WS) and all dung fibers have been investigated using Fourier Transform Infrared Spectroscopy (FTIR), scanning electron microscopy (SEM), and SEM-Energy dispersive X-ray spectroscopy (SEM-EDX). To increase paper production without damaging forest cover, it is essential to explore unconventional natural resources, such as dung fiber, which have the huge potential to produce pulp and paper, reinforcement components, etc.

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Although indigenous forest management practices have been used effectively by local people in the management and conservation of forest resources, these practices are eroding, causing negative consequences on the welfare of the people and their forests. To stem the erosion of the indigenous practices and instead stimulate, preserve, or improve their use, this study determines the socioeconomic factors that drive the household's use of the practices in the management and conservation of plant species of non-wood forest products (NWFPs). The study was carried out in Nigeria derived savannah. Data was collected from 200 randomly selected households in 10 randomly selected forest communities. Multivariate probit model was used to estimate the socioeconomic factors that influence the simultaneous use of indigenous forest management practices by households. Given multiple use of the practices, the result shows that the indigenous forest management practices used by the households are selective weeding (82.98%), controlled harvesting (82.45%), enrichment planting (75.53%), fire breaks (76.06%) and indigenous protective mechanism (45.74%). The majority (71.28%) of the respondents said they managed bush mango (Irvingia gabonensis and wombulu) using the practices, while the lowest proportion (21.28%) managed bush buck (Gongronema latifolium). The result of the multivariate probit model shows that virtually all the indigenous forest management practices are positively and significantly associated and are thus, complements. However, local protective mechanisms and controlled harvesting, local protective mechanisms and selective weeding, and local protective mechanisms and enrichment planting are not significantly associated. Farming occupation significantly increases the likelihood of simultaneous use of controlled harvesting, enrichment planting, and fire breaks as indigenous forest management practices in the management and conservation of NWFP. On the other hand, age significantly reduces the likelihood of the use of controlled harvesting and selective weeding. The study recommends the provision of support for young people who are more likely to be involved in the indigenous forest management practices; support to farmers who simultaneously use the practices, for example, through the provision of credit facilities; and a proper definition of user rights in community forests.

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This data article focuses on sustainability indicators for bioenergy generation from Brazilian Amazon׳s non-woody native biomass sources, considered to be modern forms of biomass. In the construction of the indicators, the Indicator-based Framework for Evaluation of Natural Resource Management Systems (MESMIS, from the original Spanish) method was used, with the application of the seven sustainability attributes to identify critical points and limiting and favorable factors for sustainability. The data yielded a list of 29 indicators distributed across 27 critical points, selected from three system evaluation areas: 11 environmental indicators, 11 social indicators, and 7 economic indicators.