RESUMEN

Aspergillus fungi constitute a pivotal element within ecosystems, serving as both contributors of biologically active compounds and harboring the potential to cause various diseases across living organisms. The organism's proteolytic enzyme complex, termed the degradome, acts as an intermediary in its dynamic interaction with the surrounding environment. Using techniques such as genome and transcriptome sequencing, alongside protein prediction methodologies, we identified putative extracellular peptidases within Aspergillus ochraceus VKM-F4104D. Following manual annotation procedures, a total of 11 aspartic, 2 cysteine, 2 glutamic, 21 serine, 1 threonine, and 21 metallopeptidases were attributed to the extracellular degradome of A. ochraceus VKM-F4104D. Among them are enzymes with promising applications in biotechnology, potential targets and agents for antifungal therapy, and microbial antagonism factors. Thus, additional functionalities of the extracellular degradome, extending beyond mere protein substrate digestion for nutritional purposes, were demonstrated.

Asunto(s)

RESUMEN

In order to expand the arsenal of tools and areas for practical use of BODIPY dyes as bifunctional fluorescent theranostics, we studied the effect of the meso-substituents nature and medium properties on photo- and pH-stability, efficiency of singlet oxygen generation, and affinity to biostructures of terpene-BODIPY conjugates. The BODIPYs fused with myrtenol or thiotherpenoid via carboxylic acid residues exhibit high stability over a wide pH range and the presence of a bulky substituent at the meso-position of BODIPY conjugates increases their photostability two-fold compared to structurally related meso-unsubstituted analogues. Furthermore, the photodegradation rate of the conjugates directly depends on their ability to generate singlet oxygen and the course probability of the corresponding red-ox reactions involving reactive oxygen species. The conjugate of BODIPY with a thiotherpenoid demonstrated high ability to penetrate the membranes of filamentous and yeast-like fungi and bind to membrane of organelles in the fungal cell. At the same time, this compound also had a high ability to penetrate into biofilms of Staphylococcus aureus and Klebsiella pneumoniae and into bacterial cells within the matrix, which makes this compound promising for staining intracellular structures of eukaryotic cells and bacteria embedded into biofilms.

Asunto(s)

RESUMEN

Activity of extracellular enzymes was assessed in 20 strains of microscopic fungi involved in biodegradation of technical objects exploited under tropical climate conditions (Vietnam). It was found that 19 strains possessed catalase activity, 18 strains had phenol oxidase activity, and eight strains had protease activity. The effect of industrial biocides on the activity of these enzymes was also assessed. The biocides Bior-1, Bioneutral A 10, and Bioneutral A 101 were shown to inhibit the enzymatic activity to various extent. All biocides inhibited extracellular catalase activity in most fungal strains studied. The inhibition of protease and phenol oxidase activity of same test strains was less pronounced. The response to biocides varied at the strain level; its characteristics could differ significantly even between strains of the same species. In several cases, it was observed that exposure to biocides resulted in an increase in enzyme activity.

Asunto(s)

RESUMEN

For the first time, mycological analysis of dust, including assessment of opportunistic fungal species, was carried out for the urban ecosystems of air, vegetation, and paved surfaces in different climate areas (the cities of Murmansk and Moscow). The combined effect of environmental factors (climate, functional zone, and substrate type) on qualitative and quantitative parameters of micromycete communities was assessed using MANOVA and cluster analysis. It was found that the abundance of culturable mycobiota in the air, on tree leaves, and on paved surfaces was lower in Murmansk than in Moscow. In both cities, approximately a half of fungal species were opportunistic pathogens. The relative abundance of opportunistic fungi of the BSL-2 group was higher in the air of the traffic zone in both cities and of the residential zone in Moscow. In the residential and traffic zones of Moscow, the most abundant species in the air in on the road dust were Aspergillus fumigatus and A. niger, while in Murmansk communities were dominated by members of the genera Cephalosporium, Scopulariopsis, and Trichoderma, which are less pathogenic for humans. The most significant factors affecting the abundance and species diversity of micromycetes, including opportunistic fungi, were the substrate type (air, leaves, or paved surfaces) and the climate, while the effect of the functional zone was not significant. The recreation zones of cities located in different climate regions are the most favorable for humans due to lower abundance of opportunistic fungi in the air and to lack of micromycetes of the BSL-2 and BSL-3 groups. However, the abundance of potentially pathogenic species on the surfaces of leaves and roads in this zone was higher than in the air. Therefore, it can be recommended that city residents minimize their contact with the leaves surface and road pavements, which is especially relevant for toddlers, so as to diminish the probability of encountering opportunistic mycobiota that potentially represents a health hazard.

Asunto(s)

RESUMEN

The sterol 3ß,5α,6ß,7α-tetrahydroxyergosta-8(14),22-diene was obtained from bio-guided fractioning of the chloroform extract of 50 L of liquid culture of Acremonium persicinum. This fungal strain was selected because of its anti-proliferative activity against solid human tumour cell lines (GI50 ≤ 50 µg/mL) in a bio-prospective study of fungi isolated from plant material, sediment and water samples obtained from alkaline lakes Alchichica and Atexcac in Puebla, Mexico. This compound showed GI50 (µM) values of: 16, 24, 18, 15 and 12 against tumour cell lines A-549, HBL-100, HeLa, T-47D and WiDr respectively. GI50 effects against tumour lines T-47D and WiDr were found to be greater than the clinically used drugs Etoposide and Cisplatin. Because of this, the results obtained support the pharmacological importance of the microorganisms that develop in these ecosystems and strengthen the non-invasive bio-prospection studies that our work group has developed in recent years.

Asunto(s)

RESUMEN

The studies of plant bacterial endophytes, colonizing the plant tissues without any signs of diseases, are essential for understanding of ecological interactions. The aim of our study is to detect microbiological contamination and to assess the antimicrobial, antioxidant activity, total phenolic, carotenoid content, genome size, and ploidy of non-cultivated Rosa canina sampled from urban areas. Samples of Rosa canina fruits were collected in three locations in Slovakia. The highest total viable count and the Enterobacteriaceae count in fruits were 4.32 log CFU/g and 4.29 log CFU/g, respectively. Counts of the mesophilic anaerobic sporulating bacteria, Pseudomonas spp., and of the microscopic fungi and yeasts were 3.00, 2.15 log CFU/g, 3.65 log CFU/g, and 2.76 log CFU/g, respectively. Regarding the antimicrobial activity, Escherichia coli and Klebsiela oxytoca were the most sensitive species among the assayed microorganisms to the treatment with the ethanolic extracts of Rosa canina fruits. The fruits were rich in bioactive compounds, polyphenols, and carotenoids, that could be related to their antioxidant activity. Genome sizes of analyzed samples ranged from 2.3 to 2.96. DNA-based fingerprinting obtained by iPBS markers of the Rosa canina var. lapidicola Heinr. Braun., was characterized by some distinctive inserted loci. An interdisciplinary study was performed for the dog roses from different parts of Slovakia that resulted in deeper characterization of this species.

Asunto(s)

RESUMEN

The study showed that the hen housing system and egg storage conditions had significant influence on differences in the qualitative traits of eggs. The most rapid qualitative traits changes were observed in the eggs laid by the hens kept in the free-range and litter systems. After the first week of storage fungi were found on the eggshell surface but significant growth of fungi on the eggshell surface was noted after 3 wk, regardless of the storage conditions. Higher humidity during egg storage causes the dynamic development of fungi in the albumen. The principal component analysis proved the dependency between ergosterol and the trichothecene concentration in the albumen. Mycotoxins in the albumen indicate the presence of pathogenic species of microscopic fungi. The albumen of the eggs laid by the hens kept in cages had the lowest concentration of mycotoxins. The results of the study showed that the conditions of the henhouse environment significantly influenced the initial contamination of eggshells. Egg storage conditions, i.e., high air humidity, favor the development of fungi on the eggshell surface, affect the dynamics of fungal growth into the albumen and the production of mycotoxins.

Asunto(s)

RESUMEN

Distribution and biodiversity of soil microscopic fungi in 5 areas of old environmental loads in Slovakia were studied in relation to very low amount of organic matter (%TOC from 0.2 to 3.54) and to the pH gradient from ultra-acidic (< 3.5) to very strongly alkaline (> 9.0). All soil samples were affected by several hundred years of mining activities and contained heavy metals and other toxic elements: arsenic, cadmium, copper, zinc, antimony, lead. Concentrations of toxicants highly exceeded their limited values. Fifty-three genera and 112 species of microscopic fungi were identified. Among them, Zygomycota occurred very rarely (8 genera and 12 species), except of samples with the highest content of TOC (2.01-3.54% - samples 2 and 6), regardless their pH. Though, on the other hand, from some similar samples (3, 5 and 9), incl. those with relatively high TOC (0.14-2.62%), the lower fungi were not recovered. Forty one genera and 95 species of Ascomycota represented the most abundant fungal phylum in all investigated samples. Among them, Penicillium chrysogenum var. chrysogenum, Aspergillus niger and Neosartorya fischeri were isolated the most often. Phytopathogenic moulds of Bionectria ochroleuca, Lewia infectoria, Phoma macrostoma and Phlebia acerina were also occurred frequently. The highest biodiversity of microfungal community was recorded in the extreme acidic environment, followed by the neutral, ultra-acidic and the very strong acidic ones. There was no similarity in microfungal spectrum found in the samples studied. Except of the ultra acidic and extreme acidic samples (1-2) as well as the ultra acidic and strong acidic ones (1-4) with the most rich mycobiota, that may indicate a certain similarity degree.

Asunto(s)

RESUMEN

Studies on plant metabolism, including those on cereals, increasingly focus on plant phenolic compounds, e.g. phenolic acids and flavonoids. The aim of this study was to present a comprehensive picture of major phenolic acids in grain, starting from their biosynthesis, their occurrence and finally their role in the vegetation of cereals. It is clearly connected with the polygenic plant resistance to pathogens, particularly toxin-forming fungi. Other crucial aspects include the transformations that take place during the technological processing of grain, their metabolic pathway in the human organism as well as the presentation of the health-promoting effect of grain processing products containing phenolic acids. These compounds are used as precursors of bioactive compounds commonly applied both for therapeutic purposes and in the cosmetics, engineering and food industries. An advantage of phenolic acids is the fact that they may be metabolized by microorganisms found in nature and thus they provide an alternative to the increasing load of man-made chemicals in the environment.

Asunto(s)

RESUMEN

Microbial safety is an important factor contributing to the egg quality. During egg acquisition, there is significant risk of contamination of the eggshell surface with microscopic fungi. Mycelial hyphae may grow on the eggshell surface and penetrate into the egg content. However, there is no information on the populations of microscopic fungi on the eggshell surface and, consequently, on possible production of mycotoxins. Therefore, the aim of the study was to identify the species of microscopic fungi present on the eggshell surface acquired from different breeding systems and to measure the number of selected mycotoxins. The qualitative analysis resulted in the identification of 41 isolates on the surface of eggs. There were 7 isolates from the organic production system, 11 from the free-range production system, 14 from the deep litter indoor housing system and 9 from the cage farming production system. The research proved that the diversification in the population of mycobiota on the eggshells depended on the egg-laying hen breeding system. The microscopic fungi isolated from the eggshells included toxigenic and pathogenic species such as Fusarium culmorum and F. equiseti. As the egg storage time increased, fungi, including the pathogenic species, penetrated through the eggshells. In consequence, mycotoxins were identified in the egg whites. Type-A and type-B trichothecenes were found in the eggshell samples containing F. culmorum.

Asunto(s)

RESUMEN

Highly acidic soils (pH < 3) represent an environment which might potentially offer new biotechnologically interesting fungi. Nevertheless, only little data on fungal communities in highly acidic habitats are available. Here, we focused on the diversity of cultivable filamentous microfungi in highly acidic soils (pH < 3) in the Czech Republic. Altogether, 16 soil samples were collected from four sampling sites and were processed by various approaches. In total, 54 fungal taxa were isolated and identified using classical as well as molecular markers. All dominant species were found both as living mycelia and as resistant stages. Numerous recently described or unknown taxa were isolated. The core of the fungal assemblage under study consisted of phylogenetically unrelated and often globally distributed fungi exclusively inhabiting highly acidic habitats like Acidiella bohemica, Acidomyces acidophilus, and unidentified helotialean fungus, as well as taxa known from less acidic and often extreme environments like Acidea extrema, Penicillium simplicissimum s.l., and Penicillium spinulosum. The large number of identified specialized species indicates that highly acidic environments provide suitable conditions for the evolution of specialist species. The occurrence of ubiquitous fungi in highly acidic substrates points to the principal role of competition in the colonization of such environments. The detected taxa did not require low pH to survive, because they can grow in a broad range of pH.

Asunto(s)

RESUMEN

Se determinó la calidad del aire en el interior y exterior de la Micoteca Licenciado Rúben Mayorga Peralta (MICG), el Herbario de Biología de Guatemala (Herbario BIGU), el Museo de Historia Natural (MUSHNAT) y el Museo de la Universidad de San Carlos de Guatemala (MUSAC). Para la recolección de los hongos microscópicos se utilizó la técnica volumétrica por impactación haciendo uso de un biocolector. Los muestreos fueron realizados de octubre de 2011 a marzo de 2012, la identificación microscópica se realizó por medio de preparaciones con azul de lactofenol y para la identificación de levaduras se utilizó el API 20C AUX. Los resultados obtenidos en los ambientes Micoteca Licenciado Rúben Mayorga Peralta indican que la mayor concentración fúngica fue de 1,780, (unidad formadora de colonias por metro cúbico UFC/m3) en el exterior y para el interior fue de 1,270 UFC/m3, en el caso del Herbario BIGU en el exterior fue de 2,790 UFC/m3y en el interior de 1,450 UFC/m3, para el MUSAC la mayor concentración observada fue de 990 UFC/m3 para el exterior y de 1,010 UFC/m3 para el interior, y para el MUSHNAT en el caso del ambiente exterior fue de 1,630 UFC/m3 y para el interior fue de 2,850 UFC/m3. Los géneros predominantes durante los muestreos en ambos ambientes en todas las áreas muestreadas fueron Penicillium sp., Cladosporium sp. y Aspergillus sp. Se logró el aislamiento de otros géneros fúngicos de gran importancia Fusarium sp. y Paecilomyces sp. Se llevó a cabo la elaboración de una guía que contiene información acerca de procedimientos de limpieza y bioseguridad acorde a la infraestructura y materia prima de cada establecimiento.

Air quality was determined both inside and outside the Micoteca Licenciado Rubén Mayorga Peralta (MICG), the Herbarium of the School of Biology (BIGU), the Museum of Natural History (MUSHNAT) and the Museum of the University of San Carlos of Guatemala (MUSAC). Volumetric sampling (impaction) with a bio-colector was used to gather microscopic fungi. The samples were collected from Oct 2011 to Mar 2012. Microscopic identification was performed using slides with lactophenol blue; while API 20C AUX was used for the identification of yeasts. The results obtained were 1,780 CFU/m3 outside and 1,270CFU/m3 inside for MICG; 2,790 CFU/m3 outside and 1,450 CFU/m3 inside for BIGU; 990CFU/m3 outside and 1,010 CFU/m3 inside for MUSAC, and finally 1,630 CFU/m3 outside and2,850 CFU/m3 inside for MUSHNAT. The predominant genera found in both environmentsof all sites were Penicillium sp., Cladosporium sp. and Aspergillus sp. It was also possible toisolate other extremely important fungal genera such as Fusarium sp. and Paecilomyces sp. A guide was developed with information about the proper cleaning and biosafety proceduresaccording to the infrastructure and raw materials of each site.