RESUMEN

BACKGROUND: Microgravity, a condition experienced in a spatial environment, poses unique challenges to the skeletal system, particularly in juvenile organisms. This study aimed to investigate alterations in bone biomechanics of juvenile mice due to unloading - that simulates microgravity in the laboratory-and the effects of a bone-loading intervention. We compared bone compositional and mechanical properties between 21-six-week-old C57Bl/6 from a control group (wild type) and a group that underwent a tail-suspension unloading protocol to mimic microgravity (MG). The second group (MG) experienced additional in vivo loading protocol (MG + LDG) on the right hind leg, where dynamic compressive loading was applied to the right knee using a custom-built loading device. RESULTS: Our results show that after two weeks, we successfully induced bone alterations by (i) decreasing the energy dissipated before fracture and (ii) decreasing the yield and maximum stress. In addition, we showed that Mineral to matrix component [ν1PO4/Amide I], Carbonate to Amide [CO3/Amide I], and Crystallinity [1/FWHM(ν1PO4)] are strongly linked in physiological bone but not in microgravity even after loading intervention. While Crystallinity is very sensitive to bone deformation (strain) alterations coming from simulated microgravity, we show that Carbonate to Amide [CO3/Amide I] - a common marker of turnover rate/remodeling activity-is a specific predictor of bone deformation for bone after simulated microgravity. Our results also invalidate the current parameters of the loading intervention to prevent bone alterations entirely in juvenile mice. CONCLUSIONS: Our study successfully induced bone alterations in juvenile mice by using an unloading protocol to simulate microgravity, and we provided a new Raman Spectroscopy (RS) dataset of juvenile mice that contributes to the prediction of cortical bone mechanical properties, where the degree of interrelationship for RS data for physiological bone is improved compared to the most recent evidence.

RESUMEN

Recent events in conflict zones have emphasized that the successful detection and characterisation of buried clandestine complexes, bunkers and tunnels is vitally important for forensic investigators globally, to reduce or solve criminal activities, address national security threats and avoid potential terrorist attacks. However, this can often prove very difficult, particularly in urban areas, with potentially both below-ground non target items and above-ground infrastructures present, that can interfere with detecting target(s). Here we provide selected successful case studies where forensic geoscience techniques were used to detect and characterise buried clandestine complexes, bunkers and tunnels using different geophysical techniques. Generally, desktop studies assessing pre-existing information, including local geology, soils, historical/modern remote sensing, maps and photographs inform appropriate geophysical survey technique(s) selection. Subsequent near-surface geophysical techniques are then employed to produce accurate plans of sub-surface targets, with numerical modelling and correction for the interfering effects of above ground infrastructure, enabling the calibration of geophysical datasets to provide confidence in their respective interpretations. All forensic investigations are, of course, unique to every site, and thus require an individual approach to their respective ground conditions. Investigations should be both phased and iterative, with techniques tailored to local conditions: the selection of geophysical method(s) is crucial to improve successful detection rates of such important buried targets.

RESUMEN

Propellant mass gauging under micro-gravity conditions is a challenging task due to the unpredictable position and shape of the fuel body inside the tank. Micro-gravity conditions are common for orbiting satellites and rockets that operate on limited fuel supplies. Capacitance sensors have been investigated for this task in recent years; however, the effect of various positions and shapes of the fuel body is not analyzed in detail. In this paper, we investigate this with various fill types, such as annular, core-annular, and stratified fills at different positions. We compare the performance among several curve-fitting-based approaches and a machine-learning-based approach, the latter of which offers superior performance in estimating the fuel content.

RESUMEN

An increasing number of collaborative projects between artists and scientists raises the question regarding their value, particularly when considering the redirection of resources. Here we provide a personal account of our collaborative efforts, as an artist and a scientist. We propose that one of the most significant outcomes is something that cannot be planned for in advance: serendipitous events. Such events lead to fresh perspectives and imaginative ideas, the fairy dust underlying many great works of art and science. The unexpected nature of these desired outcomes requires from us a leap of faith on the one hand, and a deep trust in our 'partner in crime' on the other.

RESUMEN

This paper describes the protocol of the microgravity experiment BEAT (Ballistocardiography for Extraterrestrial Applications and Long-Term Missions). The current study makes use of signal acquisition of cardiac parameters with a high-precision Ballistocardiography (BCG)/Seismocardiography (SCG) measurement system, which is integrated in a smart shirt (SmartTex). The goal is to evaluate the feasibility of this concept for continuous wearable monitoring and wireless data transfer. BEAT is part of the "Wireless Compose-2" (WICO2) project deployed on the International Space Station (ISS) that will provide wireless network infrastructure for scientific, localization and medical experiments.

Asunto(s)

Balistocardiografía , Balistocardiografía/métodos , Corazón , Frecuencia Cardíaca

RESUMEN

Gut microbiota, a major contributor to human health, is influenced by physical activity and diet, and displays a functional cross-talk with skeletal muscle. Conversely, few data are available on the impact of hypoactivity, although sedentary lifestyles are widespread and associated with negative health and socio-economic impacts. The study aim was to determine the effect of Dry Immersion (DI), a severe hypoactivity model, on the human gut microbiota composition. Stool samples were collected from 14 healthy men before and after 5 days of DI to determine the gut microbiota taxonomic profiles by 16S metagenomic sequencing in strictly controlled dietary conditions. The α and ß diversities indices were unchanged. However, the operational taxonomic units associated with the Clostridiales order and the Lachnospiraceae family, belonging to the Firmicutes phylum, were significantly increased after DI. Propionate, a short-chain fatty acid metabolized by skeletal muscle, was significantly reduced in post-DI stool samples. The finding that intestine bacteria are sensitive to hypoactivity raises questions about their impact and role in chronic sedentary lifestyles.

Asunto(s)

Microbioma Gastrointestinal/fisiología , Descanso/fisiología , Conducta Sedentaria , Adulto , Heces/química , Heces/microbiología , Voluntarios Sanos , Humanos , Inmersión/fisiopatología , Masculino , Propionatos/metabolismo , Simulación de Ingravidez

RESUMEN

Cell encapsulation in hydrogels has been extensively used in cytotherapy, regenerative medicine, 3D cell culture, and tissue engineering. Herein, we fabricated microencapsulated cells through microcapsules loaded with C5.18 chondrocytes alginate/chitosan prepared by a high-voltage electrostatic method. Under optimized conditions, microencapsulated cells presented uniform size distribution, good sphericity, and a smooth surface with different cell densities. The particle size distribution was determined at 150⁻280 µm, with an average particle diameter of 220 µm. The microencapsulated cells were cultured under static, shaking, and 3D micro-gravity conditions with or without bFGF (basic fibroblast growth factor) treatment. The quantified detection (cell proliferation detection and glycosaminoglycan (GAG)/type II collagen (Col-II)) content was respectively determined by cell counting kit-8 assay (CCK-8) and dimethylmethylene blue (DMB)/Col-II secretion determination) and qualitative detection (acridine orange/ethidium bromide, hematoxylin-eosin, alcian blue, safranin-O, and immunohistochemistry staining) of these microencapsulated cells were evaluated. Results showed that microencapsulated C5.18 cells under three-dimensional microgravity conditions promoted cells to form large cell aggregates within 20 days by using bFGF, which provided the possibility for cartilage tissue constructs in vitro. It could be found from the cell viability (cell proliferation) and synthesis (content of GAG and Col-II) results that microencapsulated cells had a better cell proliferation under 3D micro-gravity conditions using bFGF than under 2D conditions (including static and shaking conditions). We anticipate that these results will be a benefit for the design and construction of cartilage regeneration in future tissue engineering applications.

RESUMEN

The International Space Station (ISS) is the largest technical vehicle ever built by mankind. It provides a living area for six astronauts and also represents a laboratory in which scientific experiments are conducted in an extraordinary environment. The deployed sensor technology contributes significantly to the operational and scientific success of the station. The sensors on board the ISS can be thereby classified into two categories which differ significantly in their key features: (1) sensors related to crew and station health, and (2) sensors to provide specific measurements in research facilities. The operation of the station requires robust, long-term stable and reliable sensors, since they assure the survival of the astronauts and the intactness of the station. Recently, a wireless sensor network for measuring environmental parameters like temperature, pressure, and humidity was established and its function could be successfully verified over several months. Such a network enhances the operational reliability and stability for monitoring these critical parameters compared to single sensors. The sensors which are implemented into the research facilities have to fulfil other objectives. The high performance of the scientific experiments that are conducted in different research facilities on-board demands the perfect embedding of the sensor in the respective instrumental setup which forms the complete measurement chain. It is shown that the performance of the single sensor alone does not determine the success of the measurement task; moreover, the synergy between different sensors and actuators as well as appropriate sample taking, followed by an appropriate sample preparation play an essential role. The application in a space environment adds additional challenges to the sensor technology, for example the necessity for miniaturisation, automation, reliability, and long-term operation. An alternative is the repetitive calibration of the sensors. This approach, however, increases the operational overhead significantly. But meeting especially these requirements offers unique opportunities for testing these sensor technologies in harsh and dedicated environments which are not available on Earth, therefore pushing the related technologies and methodologies to their limits. The scientific objectives for selected experiments, representing a wide range of research fields, are presented, including the instrument setups and the implemented sensor technologies, and where available, the first scientific results are presented.

RESUMEN

Future space missions and implementation of permanent bases on Moon and Mars will greatly depend on the availability of ambient air and sustainable food supply. Therefore, understanding the effects of altered gravity conditions on plant metabolism and growth is vital for space missions and extra-terrestrial human existence. In this mini-review we summarize how plant cells are thought to perceive changes in magnitude and orientation of the gravity vector. The particular advantages of several single-celled model systems for gravity research are explored and an overview over recent advancements and potential use of these systems is provided.