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Objective: To compare the accuracy of influenza-like illness (ILI) surveillance by automatic computer analysis based on electronic medical records and by doctor's report. Methods: A total of 3 542 patients who presented to Yichang Central Hospital fever clinic, respiratory department or emergency department between April to October 2019 with an ICD-10 code for acute respiratory illness (J00-J22) and complete electronic medical information of ILI related syndromes were drawn as the study sample. Taking the classification of the study sample according to the ILI case definition by influenza surveillance professionals as the gold standard, draw the receiver operating characteristic (ROC) curve and calculate sensitivity, specificity, diagnostic consistency to compared the accuracy of ILI surveillance by automatic computer analysis and by doctor's report. Results: Median age of 3 542 cases was 30 (24, 38) years old; 1 179 cases (33.29%) compliance with the case definition, ILI reported by doctors was 1 306 cases (36.87%), and computer automatic identification ILI were 1 150 cases (32.47%); 1 391 (39.27%) cases were men. The results of automatic computer analysis and doctor report consistency of kappa values with gold standard judgment were 0.97 and 0.66 respectively; area under the ROC curve was 0.98 and 0.84, respectively. And the sensitivity and specificity of automatic computer analysis were higher than that of doctor's report (all P values were <0.001), the sensitivity was 96.95% and 82.27%, and the specificity was 99.70% and 85.78%, respectively. Conclusion: The automatic computer analysis based on electronic medical records can identified ILI cases with good sensitivity and specificity in ILI case surveillance.

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O objetivo do estudo é descrever o percurso utilizado por uma equipe de pesquisadores para elaboração e desenvolvimento de um projeto de pesquisa para avaliação da Rede de Cuidados à Pessoa com Deficiência em Minas Gerais. Trata-se de relato de experiência, no qual foram destacadas as etapas percorridas pela equipe e a importância da discussão do tema da pesquisa, bem como a realização de reuniões periódicas; dos resultados preliminares com base no estudo dos dados secundários; da elaboração dos instrumentos de coleta; da seleção de novos integrantes e realização da instrumentalização; da realização do cálculo amostral e do estudo piloto. É de relevância o registro deste percurso para que novos estudos possam reproduzir este processo e, consequentemente, gerar a comparação com resultados obtidos em outros cenários.

This study is aiming to describe the course taken by a team of researchers to elaborate and develop a research project to evaluate the Disabled People Care Network in the state of Minas Gerais. This is an experience report, in which the stages taken by the team were highlighted and the importance upon the discussion of the research topic, as well as the regular meetings occurrences; preliminary results based on the regarding the study of secondary data; the preparation of collection instruments; the selection of new members and holding the instrumentalization; the completion of the sample calculation and the pilot study. It isimportant to register the route taken in a way to allow new studies to replicate this process and thus generate the comparison with results in different scenarios.

El objetivo del estudio es describir el recorrido metodológico de un equipo de investigadores a planear y desarrollar un proyecto de investigación para evaluar la Red de cuidado de la persona con discapacidad en Minas Gerais. Se trata de un relato de experiência, dividido por pasos. Fueron aspectos destacados en el manuscrito: las medidas tomadas por el equipo, la discusión del tema de la investigación, las reuniones periódicas; los resultados preliminares sobre la base del estudio de datos secundarios; el desarrollo de los instrumentos de recolecta de datos; la capacitación de nuevos miembros; la realización del cálculo de la muestra y un estudio piloto. Es importante registrar este camino para que nuevos estudios pueden replicar este proceso y por lo tanto generar la comparación con los resultados en otros contextos.

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Objective To perform the methodological evaluation on Beckman Immage 800 automatic special protein analyzer for determining serum freeκ,λlight chains (sFLC) .Methods The Beckman Immage800 automatic special protein analyzer was used to quantitatively detect sFLC values for investigating its precision and accuracy ,analyzing its detecting range ,interference and residual contamination rate ,meanwhile verifying its reference intervals .Results The low and high values of within‐run precision coefficients of variation(CV) forκsFLC were 7 .84% and 2 .95% respectively ;which of between‐run precision CV were 7 .38% and 5 .57% re‐spectively .The within‐run precision CV for λFLC were 4 .59% and 3 .94% respectively ,which of between‐run precision CV were 3 .97% and 2 .01% respectively ;bias for detecting theκFLC andλFLC fixed quality serum and the target values was less than 5% , when the analytical detection ranges of κFLC andλFLC were 10 .8-128 .0 mg/L and 10 .1-121 .0 mg/L ,a value was 0 .95-1 .05 , r2 ≥0 .98;the carry‐over rates of κFLC andλFLC were 0 .411% and 0 .216% respectively .The interference test results showed that when free hemoglobin≤342 .0 μmol/L ,conjugated bilirubin ≤342 .0 μmol/L ,hemoglobin≤5 g/L and chyle ≤2 400 turbidness , which had no influence on sFLC results ;among 40 healthy subjects undergoing the physical examination ,1 case of κFLC detection results exceeded the reference interval recommended by the manufacturer ,while theλFLC detection values in these 40 cases were all within the reference interval recommended by the manufacturer .Conclusion The main analytical performance of the Beckman Im‐mage800 automatic special protein analyzer for detecting sFLC meets the requirements of quality objectives and can provides the sci‐entific and precise evidence of diagnosis and treatment for clinic .

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Orchid bees are increasingly applied on Neotropical biomonitoring and bioindication studies due to the relative easiness of sampling and identification when compared to other bee groups. A considerable number of orchid bee community studies have been adopting baited traps as a sampling method, especially for replication purposes. However, the trap attributes are variable, and hitherto no evaluation of different designs was carried out. Here, five attributes of baited traps were tested: trap volume, number of entrance holes, presence of landing platform, kind of landing platform, and fixation content. We use Mann-Whitney tests to access differences in richness and abundance capture rates for each trap design. We found that volume, number of entrance holes, and fixation content do not influence orchid bees capture. However, the design without landing platforms had a significantly higher capture rate for richness when compared with sanded landing platforms. On the other hand, analyzing the kind of landing platform, we detected a significantly higher richness and abundance for the trap with landing platforms glued with sand. Despite the fact that the effects of different designs tested here were very punctual, we consider that results from samples taken with different baited trap designs are comparable. Some adjustments on trap design can be done according to the particularities of future studies.

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Objective To establish a method for quantitative detection of total immunoglobulin E(TIgE) by Time resolved Flu‐oroimmunoassay(TRFIA ) .Methods The method for quantitative detection of TIgE by TRFIA was established on the basis of solidphase double sandwich enzyme linked immunosorbent assay(ELISA) .The methodology was evaluated .Results The TIgE TR‐FIA intra assay and inter assay coefficients of variation (CV) were 1 .59% -1 .68% and 5 .23% -7 .33% ,respectively .The lower limit of detection was 0 .25 IU/mL .The linear range was 1 .47-1 510 .00 IU/mL .The accuracy was within the allowable deviation ( ± 10% ) .The recovery rate was 97 .00% -106 .75% .The cross reaction test and interference experiment could meet the testing requirements .The TIgE TRFIA showed no HOOK effect at least up to 15 000 IU/mL TIgE ,compared with EUROIMMUN ELISA ,the correlation coefficient(r) was 0 .999 2(P<0 .01)for 40 blood specimens in the range of 14 .43-518 .81 IU/mL ,and the expected bias was in the range of acceptable bias (± 12 .50% ) .The reference value 100 IU/mL could be used for a normal ,allergy free adult sample TIgE level detected by TRFIA .Conclusion The established TRFIA for TIgE detection meets the demand of clini‐cal application with good precision ,high sensitivity ,wide linear range ,high accuracy ,specificity and other advantages .

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OBJECTIVE To evaluate the detect method of neopterin(Npt) by enzyme-linked immunosorbent assay(ELISA) and discuss the application of Npt in the viral encephalitis.METHODS The Npt was detected by ELISA.The methodology was investigated by sensitivity,precision,rate of recovery,interference and reference value.The npt was detected by ELISA assay in peripheral blood of 50 healthy people,30 cases of cerebrospinal fluid in children with viral encephalitis and 12 cases of cerebrospinal fluid in the control group.RESULTS In this method,within-run CVs were 4.94% and 5.55%;between-run CVs were 5.99% and 6.57%.The sensitivity was 1.08 nmol/L;the rate of recovery was 95.8-107.8%.Various indexes of the methodology coincided with the requirements of clinical laboratory.The reference value of serum Npt was 0-7.84 nmol/L.The Npt of cerebrospinal fluid was(34.09 ? 36.34) nmol/L in the viral encephalitis group,and(4.55 ? 2.89)nmol/L in the control group,and the Npt of cerebrospinal fluid in patient was significantly higher than that in control group(P

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Objective To evaluate an immunochemiluminescence assay for human plasma B-type natriuretic peptide(BNP),and to investigate the reference interval of BNP in healthy Chinese.Methods EDTA anti-coagulant plasmas from 421 healthy volunteers(202 men and 219 women) were included.BNP was measured with Triage BNP kit developed by Biosite on the Beckman Coulter Access II.Referring to the EP documents,a series of tests were designed to investigate the assay′s linearity,detection limit,sensitivity,precision,interference factors,and BNP′s stability under different storage conditions.Results The assay had a linearity of 15~5 040 ng/L,and the detection limit was 9.2 ng/L.The concentration at 10% of CV was 30 ng/L.There was no obvious interference in the assay when bilirubin was 75 years old persons was 100.7 ng/L.Conclusion The immunochemiluminescence assay for human BNP is highly sensitive.The characteristic of its wide detection linearity,precision,and anti-interference ability meets the clinical requirement.We recommend that the 97.5% percentile be a reference interval;when the age is 55 years old,the value is