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The acute phase response (APR), coordinated by a complex network of components of the immune and neuroendocrine systems, plays a key role in early immune defense. This response can be elicited by a wide variety of pathogens at different intensities (frequencies and doses), hence experimental immune challenges with antigen gradients makes it possible to evaluate sickness progression with a better representation of what occurs in natural systems. However, how infection intensity could shape the APR magnitude in wild species is still poorly understood. Here, the immune response was activated in the subterranean rodent Ctenomys talarum with a gradient of lipopolysaccharide (LPS) doses (0.5, 1, 1.5, and 2 mg/kg of body mass). Changes in body temperature, body mass, and energetic costs were evaluated over time. We also assessed cortisol levels, white blood cells counts and neutrophil: lymphocyte ratios, before and after injection. Results indicated that during the APR, C. talarum shows a hyperthermic response, which is maintained for 6 h, with slight differences among antigen doses in the pattern of thermal response and body mass change. A maximum increase in body temperature of 0.83°C to 1.63°C was observed during the first hour, associated with a metabolic cost that ranged from 1.25 to 1.41 ml O2 /gh. Although no clear effects of treatment were detected on leukocyte abundance, we found increments in neutrophil: lymphocyte ratios and gradual increases in cortisol levels corresponding to the intensity of simulated infection, which may indicate redistribution of immune cells and enhancement of immune function. An evident sickness syndrome was observed even at the lowest LPS dose that was characterized by an increase in body temperature, energy expenditure, and N: L ratio, as well as a dose-dependent increase in cortisol levels. Although in nature, other constraints and challenges could affect the magnitude and costs of immune responses, C. talarum mounts an effective APR with a low increase in their daily energy expenditure, regardless of LPS dose.

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PURPOSE: Unilateral skipping is an asymmetrical gait only exceptionally used by humans, due to high energetic demands. In skipping, the cost of transport decreases as speed increases, and the spring-mass model coexists with the vaulting pendular one. However, the mechanisms of energy transfers and recovery between the vaulting and the bouncing steps are still unclear in this gait. The objective of this work is to study how spatiotemporal and spring-mass asymmetries impact on metabolic cost, lowering it despite speed augmentation. METHODS: Kinematics and metabolic rates of healthy subjects were measured during running and skipping on a treadmill at controlled speeds. RESULTS: Metabolic power in skipping and running increased with similar slope but different intercepts. This fact determined the different behaviour of the cost of transport: constant in running, decreasing in skipping. In skipping the step time asymmetry remained constant, while the step length asymmetry decreased with speed, almost disappearing at 2.5 m/s-1. Leg stiffness in trailing limb increased with higher slope than in leading limb and running; however, the relative leg stiffness asymmetry remained constant. CONCLUSIONS: Slow skipping presents short bouncing steps, even shorter than the vaulting, impacting the stride mechanics and the metabolic cost. Faster speeds were achieved by taking longer bouncing steps and a stiffer trailing limb, allowing to improve the effectiveness of the spring-mass mechanism. The step asymmetries' trends with respect to speed in skipping open the possibility to use this gait as an experimental paradigm to study mechanisms of metabolic cost reduction in locomotion.

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PURPOSE: A controlled study evaluated the effect of condensed tannins (CT) from Gymnopodium floribundum leaf meal (GF), infection with Haemonchus contortus (I) and their interaction, on feed intake, diet digestibility and retention of N (NR) and energy (ER) in hair sheep lambs. METHODS: Thirty-six, worm-free hair sheep lambs (14.9 ± 1.56 kg body weight) were housed in metabolic cages. Eighteen animals were infected with 6000 H. contortus L3, while other 18 lambs were kept non-infected. On day 28th post-infection (PI), infected lambs were assigned to three diet groups: a diet without GF (I-NONGF), a diet with GF (I + GF) and a diet with GF + polyethylene glycol (PEG) (I + GF + PEG). Non-infected (NI) lambs were assigned to similar diet groups: NI-NONGF, NI + GF and NI + GF + PEG. The packed cell volume (% PCV), ante-mortem faecal egg counts and post-mortem worm burdens were also evaluated. RESULTS: Infection did not affect digestibility, NR and ER. Meanwhile, CT intake from the GF diet reduced the digestibility of dry matter, organic matter and crude protein, as well as NR, compared to lambs consuming the NONGF and GF + PEG diets (P < 0.05). Although, the digestible energy was similar between lambs consuming NONGF and GF + PEG diets, the ER was higher for lambs consuming the control NONGF diet. Diets did not affect the PCV, or the ante-mortem and post-mortem parasitological variables. CONCLUSION: The costs on N and energy metabolism were mainly associated with the CT content of the GF diet, but other features of the diet such as the high lignin content, seemed to affect animals consuming GF meal. Meanwhile, the H. contortus infection had a non-significant impact.

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Purpose: This study aimed to determine whether triceps surae's muscle architecture and Achilles tendon parameters are related to running metabolic cost (C) in trained long-distance runners. Methods: Seventeen trained male recreational long-distance runners (mean age = 34 years) participated in this study. C was measured during submaximal steady-state running (5 min) at 12 and 16 km h-1 on a treadmill. Ultrasound was used to determine the gastrocnemius medialis (GM), gastrocnemius lateralis (GL), and soleus (SO) muscle architecture, including fascicle length (FL) and pennation angle (PA), and the Achilles tendon cross-sectional area (CSA), resting length and elongation as a function of plantar flexion torque during maximal voluntary plantar flexion. Achilles tendon mechanical (force, elongation, and stiffness) and material (stress, strain, and Young's modulus) properties were determined. Stepwise multiple linear regressions were used to determine the relationship between independent variables (tendon resting length, CSA, force, elongation, stiffness, stress, strain, Young's modulus, and FL and PA of triceps surae muscles) and C (J kg-1m-1) at 12 and 16 km h-1. Results: SO PA and Achilles tendon CSA were negatively associated with C (r 2 = 0.69; p < 0.001) at 12 km h-1, whereas SO PA was negatively and Achilles tendon stress was positively associated with C (r 2 = 0.63; p = 0.001) at 16 km h-1, respectively. Our results presented a small power, and the multiple linear regression's cause-effect relation was limited due to the low sample size. Conclusion: For a given muscle length, greater SO PA, probably related to short muscle fibers and to a large physiological cross-sectional area, may be beneficial to C. Larger Achilles tendon CSA may determine a better force distribution per tendon area, thereby reducing tendon stress and C at submaximal speeds (12 and 16 km h-1). Furthermore, Achilles tendon morphological and mechanical properties (CSA, stress, and Young's modulus) and triceps surae muscle architecture (GM PA, GM FL, SO PA, and SO FL) presented large correlations with C.

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PURPOSE: The aim of this study was to verify whether physiological components [vertical jumps (Squat Jump - SJ and Countermovement Jump - CMJ), eccentric utilization ratio (EUR) of vertical jumps, running economy (RE), metabolic cost (C MET ), first and second ventilatory threshold (VT1 and VT2) maximal oxygen uptake (VO2MAX)] can predict maximal endurance running performance. METHODS: Twenty male runners performed maximal vertical jumps, submaximal running at constant speeds, and maximal incremental running test. Before, we measured anthropometric parameters (body mass and height) and registered the training history and volume. SJ and CMJ tests were evaluated prior to running tests. Initially, the oxygen uptake (VO2) was collected at rest in the orthostatic position for 6 min. Soon after, a 10-min warm-up was performed on the treadmill at 10 km⋅h-1, followed by two 5-min treadmill rectangular tests at 12 and 16 km⋅h-1 monitored by a gas analyzer. After that, the runners performed a maximal incremental test, where the VT1, VT2, and VO2MAX were evaluated, as well as the maximum running speed (vVO2MAX). Thus, RE and C MET were calculated with data obtained during rectangular running tests. Multivariate stepwise regression analyses were conducted to measure the relationship between independent variables (height and power of SJ and CMJ, EUR; RE and C MET 12 and 16 km⋅h-1 ; VT1, VT2, and VO2MAX), as predictors of maximal running performance (vVO2MAX), with significance level at α = 0.05. RESULTS: We found that VO2MAX and RE at 16 km⋅h-1 predict 81% of performance (vVO2MAX) of endurance runners (p < 0.001). CONCLUSION: The main predictors of the maximal incremental running test performance were VO2MAX and RE.

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Locomotion is the most common form of movement in nature. Its study allows analysis of interactions between muscle functions (motor) and lever system arrangements (transmission), thereby facilitating performance analysis of various body organs and systems. Thus, it is a powerful model to study various aspects of integrative physiology. The results of this model can be applied in understanding body functions and design principles as performance outputs of interest for medical and biological sciences. The overall efficiency (effoverall ) during locomotion is an example of an integrative parameter, which results from the ratio between mechanical output and metabolic input. Although the concepts of cost (i.e., metabolic expenditure relative to distance) and power (i.e., metabolic expenditure relative to time) are included in its calculation, the effoverall establishes peculiar relations with these variables. For a better approach to these aspects, in this study, we presented the physical-mathematical formulation of efficiency, as well as its conceptual definitions and applications. Furthermore, the concepts of efficiency, cost, and power are discussed from the biological and medical perspectives. Terrestrial locomotion is a powerful model to study integrative physiology in humans, because by analyzing the mechanical and metabolic determinants, we may verify the efficiency and economy relationship through locomotion type, and its characteristics and restrictions. Thus, it is possible to elaborate further on various improved intervention strategies, such as physical training, competition strategies, and ergogenic supplementation.

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Although studied at level surface, the trunk kinematics and pelvis-shoulder coordination of incline walking are unknown. The aim of this study was to evaluate the speed effects on pelvis-shoulder coordination and trunk movement and the cost of transport (C) during unloaded and loaded (25% of body mass) 15% incline walking. We collected 3-dimensional kinematic and oxygen consumption data from 10 physically active young men. The movements were analyzed in the sagittal plane (inclination and range of trunk motion) and the transverse plane (range of shoulder and pelvic girdle motion and phase difference). The rotational amplitude of the shoulder girdle decreased with load at all speeds, and it was lower at the highest speeds. The rotational amplitude of the pelvic girdle did not change with the different speeds. The phase difference was greater at optimal speed (3 km.hr-1, at the lowest C) in the loaded and the unloaded conditions. The trunk inclination was greater with load and increased with speed, whereas the range of trunk motion was lower in the loaded condition and decreased with increasing speed. In conclusion, the load decreased the range of girdles and trunk motion, and the pelvis-shoulder coordination seemed to be critical for the incline walking performance.

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Carnivorans are a diverse group of mammals that includes carnivorous, omnivorous and herbivorous, domesticated and wild species, with a large range of brain sizes. Carnivory is one of several factors expected to be cognitively demanding for carnivorans due to a requirement to outsmart larger prey. On the other hand, large carnivoran species have high hunting costs and unreliable feeding patterns, which, given the high metabolic cost of brain neurons, might put them at risk of metabolic constraints regarding how many brain neurons they can afford, especially in the cerebral cortex. For a given cortical size, do carnivoran species have more cortical neurons than the herbivorous species they prey upon? We find they do not; carnivorans (cat, mongoose, dog, hyena, lion) share with non-primates, including artiodactyls (the typical prey of large carnivorans), roughly the same relationship between cortical mass and number of neurons, which suggests that carnivorans are subject to the same evolutionary scaling rules as other non-primate clades. However, there are a few important exceptions. Carnivorans stand out in that the usual relationship between larger body, larger cortical mass and larger number of cortical neurons only applies to small and medium-sized species, and not beyond dogs: we find that the golden retriever dog has more cortical neurons than the striped hyena, African lion and even brown bear, even though the latter species have up to three times larger cortices than dogs. Remarkably, the brown bear cerebral cortex, the largest examined, only has as many neurons as the ten times smaller cat cerebral cortex, although it does have the expected ten times as many non-neuronal cells in the cerebral cortex compared to the cat. We also find that raccoons have dog-like numbers of neurons in their cat-sized brain, which makes them comparable to primates in neuronal density. Comparison of domestic and wild species suggests that the neuronal composition of carnivoran brains is not affected by domestication. Instead, large carnivorans appear to be particularly vulnerable to metabolic constraints that impose a trade-off between body size and number of cortical neurons.

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Objetivo: Verificar o efeito da bandagem, por meio de esparadrapo (esparadrapagem), para estabilização da inversão do tornozelo, na economia de corrida, determinada pelo custo de transporte (CT). Métodos: Dez corredores de 10 km com velocidade média de prova entre 13,5 e 15 km.h-1 realizaram os seguintes procedimentos: (1) Mensuração do consumo de oxigênio em repouso ortostático (VO2orto) por seis minutos; (2) três baterias de cinco minutos de corrida a 14 km.h-1 para determinação do VO2teste:sem esparadrapagem (SE); esparadrapagem em um tornozelo (E1T); e esparadrapagem nos dois tornozelos (E2T). O CT foi determinado segundo equação proposta na literatura. Resultados: Não houve diferenças no CT entre as baterias: SE (3,5 ± 0,2), E1T (3,6 ± 0,2) e E2T (3,6 ± 0,2) ANOVA medidas repetidas F(2;18) = 2,866; p=0,083. Conclusão: A esparadrapagem não influenciou a economia de corrida de atletas amadores de provas de longas distâncias.

Objective: To verify the effect of adhesive tape (adhesive taping) for stabilization of ankle reversals on running economy, determined by the cost of transport (CT). Methods: Ten male recreational long distance runners with average race speed between 13.5 and 15.0 km.h-1 performed the following procedures: (1) Measurement of oxygen consumption at rest (VO 2orto) for six minutes; (2) three bouts of five minutes running test at 14.0 km.h-1 to determined the Vo 2test; without taping (SE); taping in one ankle (E1T); and two ankles (E2T). Results: No difference in CT among the groups SE (3.5 ± 0.2), E1T (3.6 ± 0.2) and E2T (3.6 ± 0.2) was found (F(2;18)= 2.866; p=0.083). Conclusion: Adhesive taping does not influence the running economy in recreational long distance runners.

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