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The increase in vegetable consumption has underlined the importance of minimizing the risks associated with microbiological contamination of fresh produce. The critical stage of the vegetable washing process has proven to be a key point for cross-contamination and the persistence of pathogens. In this context, the agri-food industry has widely adopted the use of disinfectants to reduce the bacterial load in the wash water. Therefore, we conducted laboratory-scale experiments in order to demonstrate the antimicrobial activity of disinfectants used in the wash tank of agro-food industries. Different wash water matrices of shredded lettuce, shredded cabbage, diced onion, and baby spinach were treated with sodium hypochlorite (NaClO), chlorine dioxide (ClO2), and per-oxyacetic acid (PAA) at recommended concentrations. To simulate the presence of pathogenic bacteria, a cocktail of E. coli O157:H7 was inoculated into the process water samples (PWW) to determine whether concentrations of disinfectants inhibit the pathogen or bring it to a viable non-culturable state (VBNC). Hereby, we used quantitative qPCR combined with different photo-reactive dyes such as ethidium monoazide (EMA) and propidium monoazide (PMA). The results indicated that concentrations superior to 20 ppm NaClO inhibit the pathogen E. coli O157:H7 artificially inoculated in the process water. Concentrations between 10-20 ppm ClO2 fail to induce the pathogen to the VBNC state. At concentrations of 80 ppm PAA, levels of culturable bacteria and VBNC of E. coli O157:H7 were detected in all PWWs regardless of the matrix. Subsequently, this indicates that the recommended concentrations of ClO2 and PAA for use in the fresh produce industry wash tank do not inhibit the levels of E. coli O157:H7 present in the wash water.

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SUMMARY OBJECTIVE: The aim of this study was to evaluate the consumption of ultra-processed foods by Brazilian pregnant women and its association with the nutritional quality of the diet. METHODS: This is a prospective and cross-sectional study with food consumption data of Brazilian pregnant women from the 2017 to 2018 Family Budgets Survey (Pesquisa de Orçamentos Familiares). Food consumption was measured using two 24-h food recalls, and the foods were categorized according to the NOVA classification. The averages of absolute and relative energy consumption for each of the NOVA groups and subgroups were estimated. The sociodemographic characteristics described the diet's caloric contribution of ultra-processed and non-ultra-processed food fractions. Linear regression models were used to describe the association between quintiles of the caloric contribution of ultra-processed foods and the average content of nutrients in the diet. RESULTS: Consumption of ultra-processed foods represented 20.9% of the total calories in the diet of Brazilian pregnant women. There was a higher energy contribution of ultra-processed foods in the diet of pregnant women living in urban areas (22%), with higher per capita income (23.7%), and in the south region of the country (26.9%). In addition, the data showed an association between higher consumption of ultra-processed foods with reduced intake of protein, carbohydrate, fiber, potassium, iron, zinc, and folate and increased intake of total fat, saturated fat, trans fat, and free sugar. CONCLUSION: Results show that higher consumption of ultra-processed foods is associated with a reduction in the nutritional quality of the diet of Brazilian pregnant women.

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Despite evidence showing that the intake of ultra-processed food has a negative impact on health, diet quality and dietary vitamin E, its impact on vitamin E nutritional status and breast milk remains unknown. This study aimed to assess the influence of the consumption of ultra-processed foods on vitamin E biomarkers of lactating women. A cross-sectional study was performed with 294 lactating women. Food consumption was obtained by 24-h dietary recall, and foods were grouped according to the NOVA classification. Levels of α-tocopherol were analysed by HPLC. Breast milk vitamin E (BMVE) adequacy was based on the quantity of the vitamin in the estimated intake volume. The Kruskal­Wallis test was used to compare the tertiles and linear regression to association between ultra-processed food consumption and biomarkers. Ultra-processed foods accounted for 16 % of energy intake and vitamin E intakes by all women were considered low. Serum α-tocopherol was 26·55 (sd 7·98) µmol/l, 5 % (n 11) showed inadequate vitamin E (< 12 µmol/l) and 78 % had an inadequate BMVE content (< 4 mg/780 ml). The regression showed that a higher dietary share of ultra-processed foods was associated with lower concentrations of serum α-tocopherol (ß = ­0·168, 95 % CI ­0·047, 0·010, P = 0·003) and inadequate BMVE content (ß = ­0·144, 95 % CI = ­0·505, 0·063, P = 0·012) (adjustment for income and maternal age). Thus, higher dietary shares of ultra-processed foods had an impact on vitamin E biomarkers, suggesting that inadequate dietary intake practices during lactation may reduce the supply of vitamin E to women and breast milk.

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ABSTRACT Objective To investigate the consumption of processed and ultra-processed foods and evaluate its relationship with the nutritional and metabolic status of hemodialysis patients in a single center in Brazil. Methods This cross-sectional study enrolled 73 individuals in hemodialysis (50 men and 23 women, 21-87 years-old). Clinical and lifestyle variables were assessed by a semi-structured questionnaire and dietary data by food frequency questionnaire. Anthropometric and metabolic data are collected from medical records. Results Processed and ultra-processed foods represented 11.0% of daily caloric intake, 53.0% of trans fatty acid, and 12.5% of salt consumed in the study sample. Individuals who had high intake of this food group (≥128.4g/day, median intake) had higher serum phosphorus and pre-dialysis urea values (p=0.038; p=0.013, respectively). Also, individual with higher consumption of processed meat, sausages and ready prepared food had higher pre-dialysis serum urea (p=0.021), while serum potassium was higher among the subjects who consumed more sauces and salt-based seasonings (p=0.002). Conclusion Higher consumption of processed and ultra-processed foods was associated with important biomarkers of metabolic control for hemodialysis subjects, probably due to non-health dietary composition. Nutritional guidelines and intervention strategies must be promoted to reduce consumption of these food-group in thisspecific population.

RESUMO Objetivo Investigar o consumo de alimentos processados e ultraprocessados e avaliar sua relação com o estado nutricional e metabólico de pacientes em hemodiálise em um único centro brasileiro. Métodos Este é um estudo transversal com 73 indivíduos em hemodiálise (50 homens e 23 mulheres, 21-87 anos de idade). As variáveis clínicas e de estilo de vida foram avaliados por um questionário semi-estruturado, bem como dados dietéticos por questionário de frequência alimentar. Dados antropométricos e metabólicos foram coletados de registros médicos. Resultados Os alimentos processados e ultraprocessados representaram 11,0% das calorias diárias, 53,0% dos ácidos graxos trans e 12,5% de sódio consumido na amostra estudada. Os indivíduos que tiveram alta ingestão deste grupo alimentar (≥128,4 g/dia, ingestão mediana) apresentaram maiores valores de fósforo sérico e uréia pré-diálise (p=0,038; p=0,013, respectivamente). Também, indivíduos com maior consumo de carne processada, embutidos e refeições prontas apresentaram maior ureia sérica pré-diálise (p=0,021), enquanto o potássio sérico foi maior entre os indivíduos que consumiram mais molhos e temperos á base de sal (p=0,002). Conclusão O maior consumo de alimentos processados e ultraprocessados foi associado a importantes biomarcadores de controle metabólico para indivíduos em hemodiálise, provavelmente devido à composição dietética não saudável. Orientações nutricionais e estratégias de intervenção devem ser promovidas para reduzir o consumo desses alimentos nessa população específica.

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ABSTRACT Objective To investigate the consumption of processed and ultra-processed foods and evaluate its relationship with the nutritional and metabolic status of hemodialysis patients in a single center in Brazil. Methods This cross-sectional study enrolled 73 individuals in hemodialysis (50 men and 23 women, 21-87 years-old). Clinical and lifestyle variables were assessed by a semi-structured questionnaire and dietary data by food frequency questionnaire. Anthropometric and metabolic data are collected from medical records. Results Processed and ultra-processed foods represented 11.0% of daily caloric intake, 53.0% of trans fatty acid, and 12.5% of salt consumed in the study sample. Individuals who had high intake of this food group (≥128.4g/day, median intake) had higher serum phosphorus and pre-dialysis urea values (p=0.038; p=0.013, respectively). Also, individual with higher consumption of processed meat, sausages and ready prepared food had higher pre-dialysis serum urea (p=0.021), while serum potassium was higher among the subjects who consumed more sauces and salt-based seasonings (p=0.002). Conclusion Higher consumption of processed and ultra-processed foods was associated with important biomarkers of metabolic control for hemodialysis subjects, probably due to non-health dietary composition. Nutritional guidelines and intervention strategies must be promoted to reduce consumption of these food-group in thisspecific population.

RESUMO Objetivo Investigar o consumo de alimentos processados e ultraprocessados e avaliar sua relação com o estado nutricional e metabólico de pacientes em hemodiálise em um único centro brasileiro. Métodos Este é um estudo transversal com 73 indivíduos em hemodiálise (50 homens e 23 mulheres, 21-87 anos de idade). As variáveis clínicas e de estilo de vida foram avaliados por um questionário semi-estruturado, bem como dados dietéticos por questionário de frequência alimentar. Dados antropométricos e metabólicos foram coletados de registros médicos. Resultados Os alimentos processados e ultraprocessados representaram 11,0% das calorias diárias, 53,0% dos ácidos graxos trans e 12,5% de sódio consumido na amostra estudada. Os indivíduos que tiveram alta ingestão deste grupo alimentar (≥128,4 g/dia, ingestão mediana) apresentaram maiores valores de fósforo sérico e uréia pré-diálise (p=0,038; p=0,013, respectivamente). Também, indivíduos com maior consumo de carne processada, embutidos e refeições prontas apresentaram maior ureia sérica pré-diálise (p=0,021), enquanto o potássio sérico foi maior entre os indivíduos que consumiram mais molhos e temperos á base de sal (p=0,002). Conclusão O maior consumo de alimentos processados e ultraprocessados foi associado a importantes biomarcadores de controle metabólico para indivíduos em hemodiálise, provavelmente devido à composição dietética não saudável. Orientações nutricionais e estratégias de intervenção devem ser promovidas para reduzir o consumo desses alimentos nessa população específica.

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Resumo Parte-se do princípio que um sistema alimentar insustentável não é capaz de produzir alimentos saudáveis para o consumo. A alimentação só pode ser considerada saudável se for também sustentável, devendo ultrapassar a perspectiva nutricional. Assim, realizou-se uma revisão narrativa de literatura acerca de sistemas alimentares saudáveis e sustentáveis, englobando aspectos de produção, processamento, comercialização e consumo, visando levantar seus desafios e perspectivas de consolidação. A alimentação saudável e sustentável deve estar relacionada à produção de alimentos que protejam a biodiversidade e promovam o consumo variado, resgatando alimentos, preparações e hábitos culturais tradicionais. Deve ser acessível e disponível a todos, em quantidade e qualidade, baseada em alimentos produzidos e processados na região, por agricultores familiares, de maneira agroecológica, fundamentada na comercialização justa, aproximando a produção do consumo. Além disso, deve ser isento de contaminantes físicos, biológicos ou químicos que causem malefícios a todos os envolvidos, de maneira aguda ou crônica.

Abstract Based on the principle that a non-sustainable food system is not capable of producing healthy food for consumption, food can only be considered healthy if it is also sustainable, going beyond the nutritional perspective. Therefore, a narrative review of the scientific literature on the sustainable and health food system was conducted, regarding aspects of production, processing, marketing and consumption, seeking to pinpoint the challenges and perspectives for its consolidation. Food systems needs to be related to a food production and consumption system which protects biodiversity and promote a diverse consumption, bringing back traditional dishes and preparation techniques. It should also be accessible and available for everyone, both in quantity and in quality, based on food that is locally produced by family farmers through agroecology and founded on fair trade, bringing production and consumption closer together. In addition, it must be free from physical, biological or chemical contaminants that cause damage to everyone involved, whether it be an isolated incident or chronically.

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OBJECTIVE: To analyze the contribution of natural, processed and ultra-processed foods to energy and nutrient supply in Mexican households. MATERIALS AND METHODS: The database of the National Household Expenditure Survey 2013 was analyzed (n=58 001), which is a cross-sectional survey. Food supply (g/adult equivalent/day) and energy, macro- and micro-nutrient supplies were estimated. Foods were classified following the NOVA system. Households sociodemographic characteristics were analyzed as covariates. RESULTS: Natural foods (NF) contributed with more energy (55.0%) followed by ultra-processed foods (UPF, 21.2%). NF were the main source of most nutrients. Processed culinary ingredients (PCI) and processed foods (PF) had high content of energy, total fats, and saturated fats, but low content of certain micronu- trients. Sodium was mainly available in PF (34.6%) and UPF (31.4%). Sugar-sweetened beverages, fast foods, and biscuits and cookies were the main UPF in terms of energy supply. CONCLUSIONS: In Mexican households, the PCI, PF and UPF had low nutritional quality.

OBJETIVO: Analizar la contribución de los alimentos naturales, procesados y ultraprocesados a la disponibilidad de energía y nutrientes en los hogares mexicanos. MATERIAL Y MÉTODOS: Se analizó la base de datos de la Encuesta Nacional de Gasto de los Hogares 2013 (n = 58 001), la cual es una encuesta transversal. Se estimó la disponibilidad de alimentos (g/adulto equivalente/día), energía y nutrientes. Los alimentos fueron clasificados siguiendo el sistema NOVA. RESULTADOS: Los alimentos naturales (AN) y los ultraprocesados (AUP) contribuyeron con más energía. Los AN fueron la principal fuente de la mayoría de los nutrimentos. Los ingredientes culinarios procesados (ICP) y los alimentos procesados (AP) tenían alto contenido de energía, grasas totales y grasas saturadas pero bajo contenido de ciertos micronutrientes. El sodio estaba disponible principalmente en AP y AUP. Las bebidas azucaradas, comidas rápidas, galletas y panes fueron los principales AUP. CONCLUSIONES: En México, los ICP, AP y AUP tienen baja calidad nutricional.

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Abstract: Objective: To analyze the contribution of natural, processed and ultra-processed foods to energy and nutrient supply in Mexican households. Materials and methods: The database of the National Household Expenditure Survey 2013 was analyzed (n=58 001), which is a cross-sectional survey. Food supply (g/adult equivalent/day) and energy, macro- and micro-nutrient supplies were estimated. Foods were classified following the Nova system. Households sociodemographic characteristics were analyzed as covariates. Results: Natural foods (NF) contributed with more energy (55.0%) followed by ultra-processed foods (UPF, 21.2%). NF were the main source of most nutrients. Processed culinary ingredients (PCI) and processed foods (PF) had high content of energy, total fats, and saturated fats, but low content of certain micronutrients. Sodium was mainly available in PF (34.6%) and UPF (31.4%). Sugar-sweetened beverages, fast foods, and biscuits and cookies were the main UPF in terms of energy supply. Conclusions; In Mexican households, the PCI, PF and UPF had low nutritional quality.

Resumen: Objetivo: Analizar la contribución de los alimentos naturales, procesados y ultraprocesados a la disponibilidad de energía y nutrientes en los hogares mexicanos. Material y métodos: Se analizó la base de datos de la Encuesta Nacional de Gasto de los Hogares 2013 (n= 58 001), la cual es una encuesta transversal. Se estimó la disponibilidad de alimentos (g/adulto equivalente/día), energía y nutrientes. Los alimentos fueron clasificados siguiendo el sistema Nova. Resultados: Los alimentos naturales (AN) y los ultraprocesados (AUP) contribuyeron con más energía. Los AN fueron la principal fuente de la mayoría de los nutrimentos. Los ingredientes culinarios procesados (ICP) y los alimentos procesados (AP) tenían alto contenido de energía, grasas totales y grasas saturadas pero bajo contenido de ciertos micronutrientes. El sodio estaba disponible principalmente en AP y AUP. Las bebidas azucaradas, comidas rápidas, galletas y panes fueron los principales AUP. Conclusión: En México, los ICP, AP y AUP tienen baja calidad nutricional.

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INTRODUÇÃO: Distúrbios osteomusculares relacionados ao trabalho constituem o grupo de doenças ocupacionais mais registradas no Brasil, sendo as indústrias ambientes favoráveis para seu desenvolvimento. Este estudo justifica-se pela necessidade de aprofundar investigações sobre a manifestação de sintomas osteomusculares no ramo industrial agroavícola, temática pouco explorada na literatura nacional. OBJETIVOS: Analisar a prevalência de sintomas osteomusculares em trabalhadores da linha de produção e do setor administrativo de uma indústria agroavícola, caracterizar o perfil sociodemográfico e ocupacional dos dois grupos e identificar ocorrência de associações entre dor e variáveis sociodemográficas e ocupacionais. MÉTODO: Estudo correlacional e transversal, realizado por meio de caracterização sociodemográfica e ocupacional, Questionário Nórdico de Sintomas Osteomusculares e Escala Numérica de Intensidade da Dor. RESULTADOS: Participaram 154 trabalhadores do setor de produção e 24 do de administração. O único sintoma osteomuscular apresentado foi a dor, com prevalência superior a 85% para ambos os setores. Mulheres do setor de produção apresentaram 2,9 vezes mais chances de terem dor do que homens. As regiões mais afetadas no setor administrativo foram pescoço e partes inferior e superior das costas. Já no setor de produção, prevaleceram a região inferior das costas e dos ombros e a parte superior das costas. CONCLUSÕES: A dor foi descrita como o sintoma osteomuscular mais prevalente entre os trabalhadores da indústria agroavícola. Apesar das diferenças nos processos de trabalho entre os trabalhadores administrativos e produtivos, não houve diferenças estatisticamente significativas entre os setores. Por outro lado, ambos apresentaram um altíssimo nível de adoecimento, o que sugere a necessidade de aprofundamento em estudos da relação causal desses sintomas.

BACKGROUND: Work-related musculoskeletal disorders are the most frequently reported group of diseases in Brazil, their occurrence being favored by work in the industrial sector. The present study is justified by the need to investigate more thoroughly the occurrence of musculoskeletal symptoms among poultry farming workers, since this subject is scarcely explored in the Brazilian literature. OBJECTIVE: To analyze the prevalence of musculoskeletal symptoms among production and administrative employees of a poultry farming company, describe the sociodemographic and occupational profile of both groups of workers and test associations between pain and sociodemographic and occupational variables. METHODS: Cross-sectional correlational study which included sociodemographic and occupational characterization and administration of the Nordic Musculoskeletal Questionnaire and a numeric pain rating scale. RESULTS: The sample comprised 154 production employees and 24 administrative workers. Pain was the single musculoskeletal symptom reported, with prevalence of over 85% in both groups. Female production employees exhibited 2.9 times higher odds of pain compared to the males. The body sites most frequently involved were the neck and upper and lower back among the administrative employees and the shoulders and the upper and lower back among the production workers. CONCLUSION: Pain was the most prevalent musculoskeletal symptom in the analyzed sample of poultry farming workers. Statistically significant difference was not found between the groups despite their different work processes. Both groups exhibited extremely high rates of illness, which points to the need for more thorough investigation of the causal relationships of musculoskeletal symptoms

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BACKGROUND: Work-related musculoskeletal disorders are the most frequently reported group of diseases in Brazil, their occurrence being favored by work in the industrial sector. The present study is justified by the need to investigate more thoroughly the occurrence of musculoskeletal symptoms among poultry farming workers, since this subject is scarcely explored in the Brazilian literature. OBJECTIVE: To analyze the prevalence of musculoskeletal symptoms among production and administrative employees of a poultry farming company, describe the sociodemographic and occupational profile of both groups of workers and test associations between pain and sociodemographic and occupational variables. METHODS: Cross-sectional correlational study which included sociodemographic and occupational characterization and administration of the Nordic Musculoskeletal Questionnaire and a numeric pain rating scale. RESULTS: The sample comprised 154 production employees and 24 administrative workers. Pain was the single musculoskeletal symptom reported, with prevalence of over 85% in both groups. Female production employees exhibited 2.9 times higher odds of pain compared to the males. The body sites most frequently involved were the neck and upper and lower back among the administrative employees and the shoulders and the upper and lower back among the production workers. CONCLUSION: Pain was the most prevalent musculoskeletal symptom in the analyzed sample of poultry farming workers. Statistically significant difference was not found between the groups despite their different work processes. Both groups exhibited extremely high rates of illness, which points to the need for more thorough investigation of the causal relationships of musculoskeletal symptoms.

INTRODUÇÃO: Distúrbios osteomusculares relacionados ao trabalho constituem o grupo de doenças ocupacionais mais registradas no Brasil, sendo as indústrias ambientes favoráveis para seu desenvolvimento. Este estudo justifica-se pela necessidade de aprofundar investigações sobre a manifestação de sintomas osteomusculares no ramo industrial agroavícola, temática pouco explorada na literatura nacional. OBJETIVOS: Analisar a prevalência de sintomas osteomusculares em trabalhadores da linha de produção e do setor administrativo de uma indústria agroavícola, caracterizar o perfil sociodemográfico e ocupacional dos dois grupos e identificar ocorrência de associações entre dor e variáveis sociodemográficas e ocupacionais. MÉTODO: Estudo correlacional e transversal, realizado por meio de caracterização sociodemográfica e ocupacional, Questionário Nórdico de Sintomas Osteomusculares e Escala Numérica de Intensidade da Dor. RESULTADOS: Participaram 154 trabalhadores do setor de produção e 24 do de administração. O único sintoma osteomuscular apresentado foi a dor, com prevalência superior a 85% para ambos os setores. Mulheres do setor de produção apresentaram 2,9 vezes mais chances de terem dor do que homens. As regiões mais afetadas no setor administrativo foram pescoço e partes inferior e superior das costas. Já no setor de produção, prevaleceram a região inferior das costas e dos ombros e a parte superior das costas. CONCLUSÕES: A dor foi descrita como o sintoma osteomuscular mais prevalente entre os trabalhadores da indústria agroavícola. Apesar das diferenças nos processos de trabalho entre os trabalhadores administrativos e produtivos, não houve diferenças estatisticamente significativas entre os setores. Por outro lado, ambos apresentaram um altíssimo nível de adoecimento, o que sugere a necessidade de aprofundamento em estudos da relação causal desses sintomas.

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FUNDAMENTOS: El objetivo de esta investigación fue analizar las que jas de dolores y la temperatura superficial de la piel en tres sitios corporales localizados en las muñecas y manos (bilateralmente) de trabajadores expuestos a los ambientes artificialmente climatizados para verificar la relación de las quejas de dolor con la temperatura. MÉTODOS: Para la realización del estudio fueron recolectados datos cualitativos con el uso del cuestionario y datos cuantitativos con equipos de monitoreo ambiental y una cámara de detección de radia ción infrarroja. El estudio fue realizado con diecinueve trabajadores de un sector de un matadero de pollos. RESULTADOS: Los resultados apuntan que fueron identificadas meno res temperaturas superficiales de la piel, alcanzando un valor de 16,67 °C y fueron evidentes en los dedos de la mano que aseguran el producto, estando de acuerdo con el modelo adaptado de la ISO 11079 tratándose de una condición causadora de dolor. CONCLUSIONES: Podemos concluir que este modelo de negocio, en las circunstancias que se presentan, exponen a los trabajadores a condiciones que pueden causar malestar, dolor, accidentes y enfermedades profesionales relacionadas con la exposición al frío. Este resultado es preocupante debido a su potencial impacto en una mayor cobertura de la salud del trabajador, teniendo en cuenta que las estadísticas oficiales se refieren al crecimiento de este tipo de actividad debido al crecimiento del sector.

BACKGROUND: The objective of this research was to analyse the com plaints about pains and the surface temperature of the skin in three body sites located in the wrists and hands (bilaterally) of workers exposed to artificially conditioned environment to verify the relation ship of pain complaints with the temperature. METHODS: To conduct the study qualitative data were collected using a questionnaire and quantitative data using environmental monitoring equipment and an infrared radiation detecting camera. The study was carried out with nineteen workers in a chicken slaughterhouse sector. RESULTS: The results indicate that the lower surface temperatures of the skin reached 16,67 °C, were highlighted on the fingers that hold the product, considered according to the adapted model of ISO 11079 as a condition causing pain. CONCLUSION: Therefore, it can be concluded that this business model, under the presented circumstances, expose workers to conditions that may cause discomfort, pain, accidents and occupational diseases related to exposure to cold. This result is worrying due to its potential impact on the worker's health and greater coverage, considering that that official statistics refer to the growth of this type of activity due to the sector's expansion.

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Resumo: O presente estudo examina a influência do aumento da disponibilidade intradomiciliar de refrigerantes e biscoitos sobre os efeitos de uma intervenção de promoção do consumo de frutas e hortaliças. Foram analisados os dados de 70 famílias que viviam em comunidades de baixa renda na cidade do Rio de Janeiro, Brasil, que foram selecionadas por meio de uma amostra probabilística estratificada e completaram registro alimentar de 30 dias antes e depois da intervenção. A intervenção contribuiu para um aumento significativo na disponibilidade intradomiciliar de frutas e hortaliças (+2,7 p.p.; IC95%: 1,5; 4,0), superando a tendência de estagnação na população brasileira. Já a aquisição de refrigerantes e biscoitos, que não foi objeto da intervenção, acompanhou a tendência crescente de consumo destes produtos (+5,8 p.p.; IC95%: 3,3; 8,4). As famílias que aumentaram a aquisição de refrigerantes e biscoitos apresentaram menores aumentos, ou decréscimos, na aquisição de frutas e hortaliças (p < 0,05) e tiveram uma chance quase quatro vezes menor de experimentar algum aumento na disponibilidade intradomiciliar de frutas e hortaliças.

Abstract: This study examines the influence of increasing household availability of sodas and cookies on the effects of an intervention to promote the consumption of fruits and vegetables. The study analyzed data from 70 families living in low-income communities in the city of Rio de Janeiro, Brazil, selected in a stratified probabilistic sample, and who completed a 30-day food record before and after the intervention. The intervention contributed to a significant increase in the household availability of fruit and vegetables (+2.7 p.p.; 95%CI: 1.5; 4.0), contrary to the trend towards stagnation of such availability in the general population in Brazil. Meanwhile, the purchase of sodas and cookies, which was not the intervention's target, mirrored the upward trend in the consumption of these products (+5.8 p.p.; 95%CI: 3.3; 8.4). Families that increased their purchase of sodas and cookies showed lower increases, or even decreases, in the purchase of fruits and vegetables (p < 0.05), and had nearly fourfold lower odds of experiencing any increase in the household availability of fruits and vegetables.

Resumen: El presente estudio examina la influencia del aumento de la disponibilidad intradomiciliaria de refrescos y galletas, sobre los efectos de una intervención de promoción del consumo de frutas y hortalizas. Se analizaron los datos de 70 familias que vivían en comunidades de baja renta en la ciudad de Río de Janeiro, Brasil, que fueron seleccionadas mediante una muestra probabilística estratificada, y completaron un registro alimentario de 30 días antes y después de la intervención. La intervención contribuyó a un aumento significativo en la disponibilidad intradomiciliaria de frutas y hortalizas (+2,7 p.p.; IC95%: 1,5; 4,0), superando la tendencia de estancamiento en la población brasileña. Ya la adquisición de refrescos y galletas, que no fue objeto de la intervención, acompañó la tendencia creciente de consumo de estos productos (+5,8 p.p.; IC95%: 3,3; 8,4). Las familias que aumentaron la adquisición de refrescos y galletas presentaron menores aumentos, o decrementos, en la adquisición de frutas y hortalizas (p < 0,05) y tuvieron una probabilidad casi cuatro veces menor de experimentar algún aumento en la disponibilidad intradomiciliaria de frutas y hortalizas.

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Background Aspartic proteases are a subfamily of endopeptidases that are useful in a variety of applications, especially in the food processing industry. Here we describe a novel aspartic protease that was purified from Peptidase R, a commercial protease preparation derived from Rhizopus oryzae. Results An aspartic protease sourced from Peptidase R was purified to homogeneity by anion exchange chromatography followed by polishing with a hydrophobic interaction chromatography column, resulting in a 3.4-fold increase in specific activity (57.5 × 10³ U/mg) and 58.8% recovery. The estimated molecular weight of the purified enzyme was 39 kDa. The N-terminal sequence of the purified protein exhibited 63-75% identity to rhizopuspepsins from various Rhizopus species. The enzyme exhibited maximal activity at 75°C in glycine-HCl buffer, pH 3.4 with casein as the substrate. The protease was stable at 35°C for 60 min and had an observed half-life of approximately 30 min at 45°C. Enzyme activity was not significantly inhibited by chelation with ethylenediamine tetraacetic acid (EDTA), and the addition of metal ions to EDTA-treated protease did not significantly change enzyme activity, indicating that proteolysis is not metal ion-dependent. The purified enzyme was completely inactivated by the aspartic protease inhibitor Pepstatin A. Conclusion Based on the observed enzyme activity, inhibition profile with Pepstatin A, and sequence similarity to other rhizopuspepsins, we have classified this enzyme as an aspartic protease.

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O açaí é uma fruta típica da Amazônia, cuja planta é uma palmeira tropical que se desenvolve principalmente no norte do Brasil, porém atualmente com desenvolvimento em outras regiões e outros países como a Venezuela. A polpa de açaí tem sido submetida ao processo de desidratação, que além de ser utilizado como um método de conservação, impedindo a deterioração e perda do valor comercial, objetiva o beneficiamento do alimento, possibilitando de instalação de um novo produto no mercado. A operação de secagem é o método de conservação de alimentos mais antigo e está entre os mais importantes, mas apresenta algumas desvantagens como alto consumo de energia e emprego de temperaturas elevadas. As características finais de produtos desidratados, como o pó de açaí, dependem de algumas variáveis de processo, tais como as características reológicas, como a viscosidade. Portanto, é importante que estas variáveis sejam estudadas, a fim de se obter produtos com melhor qualidade de solubilidade e reconstituição do pó e melhor efetividade no processo industrial. Este trabalho teve como objetivo avaliar o efeito da temperatura no comportamento reológico de pó de açaí reconstituído. As medidas reológicas foram feitas nas temperaturas de 8, 15, 25, 35 e 45º C. Os modelos de Newton e Casson foram ajustados aos parâmetros reológicos através de regressão não linear. Para todas as temperaturas estudadas, observou-se que o aumento da taxa de deformação acarretou aumento da tensão de cisalhamento. O pó de açaí reconstituído, nas diferentes temperaturas estudadas, apresentou comportamento Newtoniano.

Açaí is a typical fruit from the Amazon. The açaí plant is a tropical palm tree that grows mainly in North of Brazil, but with current development in other regions and countries such as Venezuela. The pulp of açaí has been submitted to dehydration process, used as a method of food preservation, which avoids spoilage and commercial losses and also aim at the food improvement. The drying operation is the oldest and one of the most important methods for food preservation, but it has some disadvantages like high energy consumption and use of high temperatures. The final characteristics of dehydrated products like the açaí powder depend on some process variables, such as rheological properties, like the viscosity. Thus, it is important to study these variables in order to obtain products with better quality. This study aimed to evaluate the effect of temperature on the rheological behavior of reconstituted açaí powder. The rheological measurements were carried out at 8, 15, 25, 35 and 45 °C. Newton and Casson models were fitted to rheological parameters using nonlinear regression. For all temperatures studied, it was observed that the increase of the shear rate resulted in an increase of the shear stress. The reconstituted açaí powder studied at different temperatures showed Newtonian behavior.

RESUMEN

Protective clothing is required in the food-processing industry, to protect workers from contamination by bacteria, fungi, viruses, prions etc. contained in the secretions and raw meat of slaughtered animals, and to protect the meat from being contaminated by microorganisms carried by the workers. It is well-understood that textiles are a control point (CP), and must be appropriately cleaned and disinfected in order to prevent biocontamination. Although the laundering procedure itself is important for achieving disinfection, it is also essential to maintain an appropriate hygiene level in the laundry, in order to prevent recontamination of textiles by environmental viable microorganisms. In this study, a sanitary-microbiological analysis was carried out in selected CPs in two laundries. Chemo-thermal washing efficiency was determined by evaluating the anti-bacterial effect against Enterococcus faecium and Staphylococcus aureus. The hygienic state of the laundries was determined by evaluating the number and type of microorganisms at selected CPs throughout the whole laundering procedure. The results indicated that the sanitary condition of both laundries did not reach the required levels and that several microbes were resistant to cleaning and disinfecting agents. It is obvious from the results that achievement of an appropriate hygiene level during laundering textiles from the food processing industry requires the implementation of appropriate corrective monitoring measures.

Na indústria de alimentos é necessário o uso de roupas de proteção, para proteger os trabalhadores da contaminação por bactérias, fungos, virus, prions, etc, encontrados nas secreções e carne dos animais abatidos, assim como proteger a carne da contaminação com microrganismos carreados pelos trabalhadores. Os têxteis são um Ponto de Controle (PC), e devem ser limpos e desinfetados de forma adequada para prevenir a biocontaminação. Embora o processo de lavagem seja importante para obter a desinfecção, é também essencial manter um nível apropriado de higiene dentro da lavanderia para prevenir recontaminação dos têxteis com microrganismos do ambiente. Nesse trabalho, realizou-se uma análise microbiológica de Pontos Críticos de duas lavanderias. A eficiência da lavagem termoquímica foi determinada através da análise do efeito antibacteriano contra Enterococcus faecium e Staphylococcus aureus. A higiene nas lavanderias foi avaliada através da determinação do número e tipos de microrganismos presentes em PCs selecionados no processo de lavagem. Os resultados indicaram que as condições de higiene nas duas lavanderias não atingiram os níveis necessários, e que vários microrganismos apresentaram resistência aos agentes sanificantes e de limpeza, indicando a necessidade de implementação de medidas corretivas apropriadas.