RESUMEN

The aim of this work was to study the, so far, unexplored possibility that non-genetic inheritance of animal behavioral characteristics could depend on the state of the parents at the time of conception. In this study, we measured the levels of motor and exploratory activity in rats at the ages of 2 and 5 months. Male and female rats were mated at the age of 5 months. The following groups were used: male and female rats with high motor activity at ages of 2 and 5 months (ACT+); male and female rats with high activity at the age of 2 months, but low activity at the age of 5 months (ACT-); male and female rats with low activity at the ages of 2 and 5 months (PAS-); male and female rats with low activity at the age of 2 months, but high activity at the age of 5 months (PAS+). It was found that both males and females ACT+ had significantly higher motor activity, which was observed in the first 10minutes, in the next 20-60minutes, in the center of the cage and more rearings as compared with PAS-rats. Significant differences in the severity of exploratory activity were found between the male offspring of ACT+ and ACT- rats. Differences between the offspring of PAS+ and PAS- rats were observed in both the male and female rats. The motor activity of animals in the period from 20minutes after the start of registration did not differ between groups. Thus, it can be considered that individual characteristics of general motor activity are due to genetically inherited factors, while differences in the level of exploratory activity, apparently, are formed due to non-genetic influences from parents during mating.

RESUMEN

Understanding how both visual and contextual in-game information influences player's attempts to gain an advantage over their opponent is key to understanding skilled decision-making in fast-ball sports. In the present study, we conducted semi-structured interviews with 15 male professional football players to explore their reported behaviours and perspectives on their in-game decision-making and the ways in which they adapt to gain an advantage over their opponent. Professional players who have competed internationally at either Under 17, Under 19, Under 21, or senior level took part in semi-structured interviews. The data were analysed using reflexive thematic analysis which generated four higher-order themes about players reported behaviours and perspectives on their decision-making and ways of gaining an advantage: (i) being "unpredictable"; (ii) option generation and invitation, comprising two sub-themes: (iia) generating and realising options in action; and (iib) act on what invites you in the moment; (iii) anticipation and awareness; and (iv) dictating and controlling the game. A key finding was that players attempts to gain an advantage were largely grounded in the aim of 'being unpredictable' through the use of deception and disguise, as well as deliberate manipulation of action sequences in order to make their actions 'hard to read'. A further important finding was that players do not always seek a universal optimal decision, as their individual action capabilities influence their available options. These findings have important implications for the assessment of decision-making performance in future empirical research.

RESUMEN

In orbital and ground-based experiments, it has been demonstrated that ionizing radiation (IR) can stimulate the locomotor and exploratory activity of rodents, but the underlying mechanism of this phenomenon remains undisclosed. Here, we studied the effect of combined IR (0.4 Gy γ-rays and 0.14 Gy carbon-12 nuclei) on the locomotor and exploratory activity of rats, and assessed the sensorimotor cortex volume by magnetic resonance imaging-based morphometry at 1 week and 7 months post-irradiation. The sensorimotor cortex tissues were processed to determine whether the behavioral and morphologic effects were associated with changes in neurotrophin content. The irradiated rats were characterized by increased locomotor and exploratory activity, as well as novelty-seeking behavior, at 3 days post-irradiation. At the same time, only unirradiated rats experienced a significant decrease in the sensorimotor cortex volume at 7 months. While there were no significant differences at 1 week, at 7 months, the irradiated rats were characterized by higher neurotrophin-3 and neurotrophin-4 content in the sensorimotor cortex. Thus, IR prevents the age-associated decrease in the sensorimotor cortex volume, which is associated with neurotrophic and neurogenic changes. Meanwhile, IR-induced increases in locomotor activity may be the cause of the observed changes.

Asunto(s)

RESUMEN

Differences in individual behaviour within a group can give rise to functional dissimilarities between groups, particularly in social animals. However, how individual behavioural phenotypes translate into the group phenotype remains unclear. Here, we investigate whether individual behavioural type affects group performance in a eusocial species, the ant Aphaenogaster senilis. We measured individual behavioural traits and created groups of workers with similar behavioural type, either high-exploratory or low-exploratory workers. We tested these groups in four different, ecologically relevant, tasks: reaction to an intruder, prey retrieval from a maze, nest relocation and tool use. We show that, compared to groups of low-exploratory workers, groups of high-exploratory workers were more aggressive towards intruders, more efficient in collecting prey, faster in nest relocation and more likely to perform tool use. Our results demonstrate a strong link between individual and collective behaviour in ants. This supports the 'behavioural type hypothesis' for group dynamics, which suggests that an individual's behaviour in a social environment reflects its own behavioural type. The average behavioural phenotype of a group can therefore be predicted from the behavioural types of individual group members.

Asunto(s)

RESUMEN

Various studies have shown a relationship between the development of language and object play in children. Children with Autism spectrum disorder (ASD) show difficulties in both of these areas of functioning. But the knowledge about the relationships between the severity of ASD symptoms and object play in children at risk of developmental disorders remains limited. To explore these relationships, 44 children aged 13-37 months took part in this study. Object play and ASD symptoms were assessed in two age groups (13-18 months and 24-37 months). The results show that ASD symptom intensity is related to object play complexity in children at risk of developmental disorders. However, these relationships are different depending on the age of the children.

RESUMEN

Chronic stress due to social isolation (SI) can lead to distress with negative consequences for both humans and animals. Numerous disorders caused by SI include disorders in the emotional-motivational domain and cognitive functions, as well as changes in social behavior. There are currently no data identifying the sequelae of SI when its duration is significantly increased. Although female rats have been shown to be highly sensitive to stress, research on them is lacking. The present study assessed sociability and preference for "social novelty" in a three-chamber social test in female Wistar rats in two series of experiments at different time points during prolonged SI, which began at adolescence and continued to ages 5.5 and 9.5 months. At two months of SI, rats showed an increased preference for a social object over a non-social object (increased sociability) simultaneously with the appearance of signs of a decrease in the preference for a new social object over an already familiar social object (signs of a decrease in the preference for social novelty). In a social interaction test, the rats also displayed increases in the durations of social contacts, including aggressive interactions; they showed a decrease in exploratory risk assessments (head dips from the open arms) in the elevated plus maze test and a decrease in exploratory activity. After SI lasting 8.5 months, the rats showed signs of social deficit and a marked decrease in the preference for social novelty. No signs of increased aggressiveness were found. Thus, the impact of SI on social behavior depended on its duration and, we believe, was accompanied by a change in coping strategies.

RESUMEN

We studied exploratory activity and learning ability in sexually mature male rhesus monkeys (Macaca mulatta) and hamadryas baboons (Papio hamadryas). The interspecies differences were analyzed by the following parameters: the level of exploratory activity, diversity of exploratory activity, concentration on the object, learning ability, training levels, and dynamics of learning. The studied group of hamadryas baboons showed higher levels of exploratory activity and learning ability than the group of rhesus monkeys.

Asunto(s)

RESUMEN

Objectives: The impact of chronic exposure to environmental adversities on brain regions involved in cognition and mental health depends on whether it occurs during the perinatal period, childhood, adolescence or adulthood. The effects of these adversities on the brain and behavior arise as a function of the timing of the exposure and their co-occurrence with the development of specific regions. Here we aimed to explore the behavioral phenotypes derived from two nutritional stress paradigms which differed in the timing of exposure: a low-protein perinatal diet during gestation and lactation and a low-protein diet during adolescence.Methods: Locomotor and exploratory activity, recognition memory and aversive memory were measured in CF-1 8-week-old male mice subjected to perinatal malnutrition (LP-P) or adolescent malnutrition (LP-A), and their respective controls with normal protein diet (NP-P and NP-A).Results: By using the open field test, we found that LP-P and LP-A mice showed reduced exploratory activity compared to controls, but no alterations in their locomotor activity. Recognition memory was impaired only in LP-P mice. Interestingly, aversive memory was not altered in LP-P mice but was enhanced in LP-A mice. Considering the stress-inoculation theory, we hypothesized that protein malnutrition during adolescence represents a challenging but still moderate stressful environment, which promotes active coping in face of later adversity.Conclusion: Our results indicate that while perinatal malnutrition impairs recognition memory, adolescent malnutrition enhances aversive memory, showing dissimilar adaptive responses.

Asunto(s)

RESUMEN

The shorter life spans of mice provide an exceptional experimental gerontology scenario. We previously described increased bizarre (disruptive) behaviors in the 6-month-old 3xTg-AD mice model for Alzheimer's disease (AD), compared to C57BL/6J wildtype (NTg), when confronting new environments. In the present work, we evaluated spontaneous gait and exploratory activity at old age, using 16-month-old mice. Male sex was chosen since sex-dependent psychomotor effects of aging are stronger in NTg males than females and, at this age, male 3 × Tg-AD mice are close to an end-of-life status due to increased mortality rates. Mice's behavior was evaluated in a transparent test box during the neophobia response. Stretching, jumping, backward movements and bizarre circling were identified during the gait and exploratory activity. The results corroborate that in the face of novelty and recognition of places, old 3xTg-AD mice exhibit increased bizarre behaviors than mice with normal aging. Furthermore, bizarre circling and backward movements delayed the elicitation of locomotion and exploration, in an already frail scenario, as shown by highly prevalent kyphosis in both groups. Thus, the translational study of co-occurrence of psychomotor impairments and anxiety-like behaviors can be helpful for understanding and managing the progressive functional deterioration shown in aging, especially in AD.

Asunto(s)

RESUMEN

Introduction: Single housing of laboratory mice is a common practice to meet experimental needs, or to avoid intermale aggression. However, single housing is considered to negatively affect animal welfare and may compromise the scientific validity of experiments. The aim of this study was to investigate whether the use of a cage with a cage divider, which avoids physical contact between mice while maintaining sensory contact, may be a potential refinement strategy for experiments in which group housing of mice is not possible. Methods: Eight-week-old male C57BL/6JRj mice were single housed, pair housed or pair housed with a cage divider for four (experiment 1) or ten (experiment 2) weeks, after which we performed an open field test, Y-maze spontaneous alternation test, elevated plus maze test, an auditory fear conditioning task, and assessed responsiveness of the hypothalamic-pituitary-adrenal (HPA) axis. Results: Housing conditions did not affect body weight, exploratory activity, anxiety, working memory, fear memory processing or markers for HPA-axis functioning in either experiment 1 or experiment 2. There was an increased distance traveled in mice housed with a cage divider compared to pair housed mice after 4 weeks, and after 10 weeks mice housed with a cage divider made significantly more arm entries in the Y-maze spontaneous alternation test. Conclusion: Taken together, our study did not provide evidence for robust differences in exploratory activity, anxiety, working memory and fear memory processing in male C57BL/6JRj mice that were single housed, pair housed or pair housed with a cage divider.

RESUMEN

Accumulating clinical and preclinical data indicate a prominent role of gut microbiota in regulation of physiological functions. The gut-brain axis imbalance due to gut dysbiosis is associated with a range of neurodegenerative diseases. Probiotics were suggested not only to restore intestinal dysbiosis but also modulate stress response and improve mood and anxiety symptoms. In this study, we assessed the effects of probiotic lactobacilli on behavioral reactions, the level of oxidative stress and microbiota content in mice administered to broad-spectrum antibiotics. Our study demonstrates that antibiotic treatment of adolescent mice for two weeks resulted in higher mortality and lower weight gain and induced significant changes in behavior including lower locomotor and exploratory activity, reduced muscle strength, visceral hypersensitivity, higher level of anxiety and impaired cognitive functions compared to the control group. These changes were accompanied by decreased diversity and total amount of bacteria, abundance of Proteobacteria and Verrucomicrobia phyla, and reduced Firmicutes/Bacteroides ratio in the gut microbiota. Moreover, a higher level of oxidative stress was found in brain and skeletal muscle tissues of mice treated with antibiotics. Oral administration of two Lactobacillus strains prevented the observed changes and improved not only microbiota content but also the behavioral alterations, suggesting a neuroprotective and antioxidant role of probiotics.

RESUMEN

Neuropeptide S (NPS) is a peptide neurotransmitter that in animal studies promotes wakefulness and arousal with simultaneous anxiety reduction, in some inconsistency with results in humans. We examined the effect of NPS on rat ultrasonic vocalizations (USV) as an index of affective state and on behaviour in novel environments in rats with persistent inter-individual differences in exploratory activity. Adult male Wistar rats were categorised as of high (HE) or low (LE) exploratory activity and NPS was administered intracerebroventricularly (i.c.v.) at a dose of 1.0 nmol/5 µL, after which USVs were recorded in the home-cage and a novel standard housing cage, and behaviour evaluated in exploration/anxiety tests. NPS induced a massive production of long and short 22 kHz USVs in the home cage that continued later in the novel environment; no effect on 50 kHz USVs were found. In LE-rats, the long 22 kHz calls were emitted at lower frequencies and were louder. The effects of NPS on behaviour appeared novelty- and test-dependent. NPS had an anxiolytic-like effect in LE-rats only in the elevated zero-maze, whereas in HE-rats, locomotor activity in the zero-maze and in a novel standard cage was increased. Thus NPS appears as a psychostimulant peptide but with a complex effect on dimensions of affect.

RESUMEN

Maximizing performance success in sports is about continuous learning and adaptation processes. Aside from physiological, technical and emotional performance factors, previous research focused on perceptual skills, revealing their importance for decision-making. This includes deriving relevant environmental information as a result of eye, head and body movement interaction. However, to evaluate visual exploratory activity (VEA), generally utilized laboratory settings have restrictions that disregard the representativeness of assessment environments and/or decouple coherent cognitive and motor tasks. In vivo studies, however, are costly and hard to reproduce. Furthermore, the application of elaborate methods like eye tracking are cumbersome to implement and necessitate expert knowledge to interpret results correctly. In this paper, we introduce a virtual reality-based reproducible assessment method allowing the evaluation of VEA. To give insights into perceptual-cognitive processes, an easily interpretable head movement-based metric, quantifying VEA of athletes, is investigated. Our results align with comparable in vivo experiments and consequently extend them by showing the validity of the implemented approach as well as the use of virtual reality to determine characteristics among different skill levels. The findings imply that the developed method could provide accurate assessments while improving the control, validity and interpretability, which in turn informs future research and developments.

Asunto(s)

RESUMEN

The purpose of this study was to analyse the scanning behaviour of elite youth football players across different playing positions and age groups during high-level matches. Data was obtained by filming the 2018 UEFA European U17 and U19 Championship semi-finals and finals. A total of 53 outfield players from the four teams that reached the finals were analysed in both their respective semi-final and final matches, resulting in a total of 1686 attacking play situations. Ecological psychology provided us with the theoretical rationale for the study and informed our research hypotheses and interpretations. We found that U19 players performed more scans than U17 players. A positive relationship between scan frequency and pass success was also found. The results further suggest that opponent pressure and pitch position are both critical contextual factors that may influence scanning behaviour. In addition, central midfielders and central defenders were found to have higher scan frequencies than players in other positions. Our results support and extend previous research, suggesting that playing positions and age groups are important factors that impact visual perception and specifically scanning in football. Potential implications for coaches and recommendations for future studies are discussed.

Asunto(s)

RESUMEN

Cognitive abilities of an animal can be influenced by distinct social experiences. However, the extent of this modulation has not been addressed in different learning scenarios: are all tasks similarly affected by social experiences? In the present study, we analyzed the effect of social dominance in aversive and appetitive memory processes in the crab Neohelice granulata. In addition, we studied the influence of social isolation on memory ability. Social dominance experiments consisted of an agonistic phase immediately followed by a memory phase. During the agonistic phase, matched pairs of male crabs were staged in 10-min encounters and the dominant or subordinate condition of each member of the dyad was determined. During the memory phase, crabs were trained to acquire aversive or appetitive memory and tested 24 h later. Results showed that the agonistic encounter can modulate long-term memory according to the dominance condition in such a way that memory retention of subordinates results higher than their respective dominant. Remarkably, this result was found for both aversive and appetitive memory tasks. In addition, we found that isolated animals showed no memory retention when compared with animals that remained grouped. Altogether this work emphasizes the importance of social context as a modulator of cognitive abilities.

Asunto(s)

RESUMEN

Several studies have used zebrafish to investigate the effects of environmental enrichment on behavior and physiology. However, to date there are no studies evaluating the behavioral responses, such as habituation and exploration, of enriched-housed zebrafish when they are submitted to novelty paradigms. The present work was, therefore, designed to evaluate the habituation and exploratory responses of zebrafish exposed to enriched- (EE) and non-enriched (NE) environments when they face novelty. Adult wild-type zebrafish were used. Three different enriched contexts were designed. In Context 1, zebrafish was exposed to enrichment during 7 days, which reduced their total distance traveled in novel tank and social preference tests in comparison to the non-enriched animals. In Context 2, animals were exposed to same enrichment during 14 days. EE exposure did not alter the behavioral responses of zebrafish compared to NE. In Context 3, fish were exposed to enrichment during 14 days, with changing the enriching elements at day 8. Similarly to Context 1, total distance traveled was reduced by EE exposure when compared to NE. Our results suggest a modulatory effect of EE on adult zebrafish locomotion that may be dependent on the time of exposure and on the physical structure of the enriched environment.

Asunto(s)

RESUMEN

The fundamental difference between the enactive approach and Gibson's ecological approach lies in the view toward our shared environment. For Varela et al. (1991), a pregiven environment that exists "out there" is incompatible with the worlds enacted by various histories of life. For Gibson (1979/2015), the environment with its unlimited possibilities that exists out there offers many ways of life. Drawing on the recent empirical studies on the mechanical basis of information and pattern formation in a wide range of fields, this paper illustrates a principle regarding how pattern and change that are formed in an environmental medium, under certain conditions, could serve as the reservoir of information that makes available a variety of opportunities for perception. The second part of this paper offers a discussion about how the consideration of the materials that make up the terrestrial environment-the particles in the atmosphere and the textured surfaces-led Gibson to replace the concept of "space" with the notion of "medium" that allows for the open-ended activities of perception. Finally, I argue that given due consideration of the ambient information available in the medium, the apparent incompatibility between the world independent of the perceiver that exist out there and the worlds enacted by various histories of life could be resolved.

RESUMEN

Pain measures traditionally used in rodents record mere reflexes evoked by sensory stimuli; the results thus may not fully reflect the human pain phenotype. Alterations in physical and emotional functioning, pain-depressed behaviors and facial pain expressions were recently proposed as additional pain outcomes to provide a more accurate measure of clinical pain in rodents, and hence to potentially enhance analgesic drug development. We aimed to review how preclinical pain assessment has evolved since the development of the tail flick test in 1941, with a particular focus on a critical analysis of some nonstandard pain outcomes, and a consideration of how sex differences may affect the performance of these pain surrogates. We tracked original research articles in Medline for the following periods: 1973-1977, 1983-1987, 1993-1997, 2003-2007, and 2014-2018. We identified 606 research articles about alternative surrogate pain measures, 473 of which were published between 2014 and 2018. This indicates that preclinical pain assessment is moving toward the use of these measures, which may soon become standard procedures in preclinical pain laboratories.

Asunto(s)

RESUMEN

Olfaction is an essential sensory modality of insects which is known to vary with age. In short-lived insects odour response generally declines rapidly with increasing age, but how increasing age affects the olfactory response of long-lived insects is less known and there may be different life-time patterns of olfactory response. Here, we examine the effect of age on olfactory response and exploratory activity of a long-lived tephritid fruit fly, Bactrocera tryoni from sexual maturity (3 weeks) to advanced age (15 weeks). Males were tested against a male-specific attractant, cue-lure, which is associated with courtship and sexual selection in this species; while females were tested against guava-juice, a highly attractive oviposition host fruit odour. Trials were done in the laboratory using a Y-tube olfactometer at three weekly intervals. The probability of olfactory response of both males and females to tested odours declined with age. Males retained a constant attraction to cue-lure until 12 weeks of age, but then showed a significant drop in olfactory response at 15 weeks. However, females showed the highest attraction to guava-juice odour until six weeks of age and declined gradually thereafter. The change on odour response over time can be associated with an age-related change in initial locomotor activity for females as there was no change, over the life of the experiment, in selective female orientation to the odour source once flies started exploring within the olfactometer. However, for 15 week-old males, there was a simultaneous drop in both locomotor activity and selective olfactory orientation. The consistent attraction of male to cue-lure might be related to life-long reproductive activities of males, as males are thought to mate continuously during life. On the other hand, females' highest attraction to guava-juice odour in early life followed by a gradual decline might be linked with their oviposition rate which peaks in early life.

Asunto(s)

RESUMEN

Research on exploratory behavior plays a key part in behavioral science. Studying exploratory behavior of laboratory rodents may provide important data about many developmental and neurobiological processes occurring in animal ontogenesis. The proposed protocol for measuring the free (low-stress) exploration behavior in rats is straightforward, requires minimal resources and very little animal training. It can therefore be broadly applied to studying animal cognition, animal behavior in general, the aging processes, and several animal models of various phenomena.