RESUMEN
There is currently no consensus for the quantification and identification of weak gammaglobulin monoclonal spike. We conducted a study of 12 months including 410 serum protein electrophoresis (SPE) with suspected weak monoclonal spike in gammaglobulin area without anteriority, which led to the realization of an immunofixation (IF); 276 SPE has a weak spike in gammaglobulins (defined with perpendicular drop method (orthogonal (O) quantification <5 g/L) but only 157 (57%) has monoclonal immunoglobulin by immunofixation of serum. The monitoring of 50 true positive monoclonal immunoglobulins showed the stability of the spike at more than half of the patients and its disappearance in 28% of cases. The number of true positives spike raise when using the tangent skimming method quantification (T) rather than the orthogonal method (O). The distribution of true positives based on T/O ratio propose a 6% threshold below which a monoclonal immunoglobulin is rarely found (VPNR>6%=73.7%), and a 10% threshold above more than 80% of true positives are detected (VPPR>10%=94.4%). The use of the T/O ratio could i) help to predict the presence of an IM when low intensity spike is detected, and help to choose between the realization of an immunofixation or a control of SPE later; ii) be a key tool for an inter-laboratory harmonization of IM follow up.
Asunto(s)
Inmunoglobulina G/análisis , Paraproteinemias/sangre , Anciano , Anciano de 80 o más Años , Estudios Transversales , Electroforesis/métodos , Femenino , Francia , Humanos , Inmunoglobulina G/sangre , Masculino , Persona de Mediana EdadRESUMEN
A significant role is played in inflammation by the liver, which, stimulated by inflammatory mediators, synthetizes plasma proteins with various dynamics. The purpose of these studies is to generate a detailed dynamic analysis of changes to concentrations of plasma and serum protein fractions and selected acute-phase proteins as well as nonspecific biochemical indices during the course of an induced pleurisy. The studies were conducted on female inbred Buffalo rats, which were divided into two groups: a control group (C) and an experimental group (IP) in which pleurisy was induced. In the IP group, significant changes in biochemical indices were observed between the 48th and 96th hours of pleurisy. A reduction of albumin, transferrin, urea, and creatinine concentrations was observed, while concentrations of the complement components C3 and C4, haptoglobin, and fibrinogen increased. An early increase of IL-1 was observed, while increases of IL-6 and TNF were noted in the later period. The maximum intensity of the processes described above occurred between the 72nd and 96th hours of pleurisy.