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Recently, fish consumption has been increasing; subsequently, the number of by-products has also increased. However, generated residues are frequently discarded, and an appropriate management is necessary to properly use all fish by-products. Fishery by-products are well known for their content of bioactive compounds, such as unsaturated fatty acids, amino acids, minerals, peptides, enzymes, gelatin, collagen, and chitin. Several studies have reported that fishery by-products could provide significant properties, including antioxidant, antihypertensive, antimicrobial, anti-inflammatory, and antiobesity. Consequently, fish discards are of considerable interest to different industrial sectors, including food, nutraceuticals, medical, and pharmacology. In the food industry, the interest in using fishery by-products is focused on hydrolysates as food additives, collagen and gelatin as protein sources, chitin and chitosan to form edible films to protect food during storage, and oils as a source of Omega-3 and useful as antioxidants. Although different studies reported good results with the use of these by-products, identifying new applications in the food sector, as well as industrial applications, remains necessary.

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The continuous discharge of pharmaceutical compounds into the aquatic environment has raised concerns over the contamination of water resources. Urban activities and intensive animal breeding are important sources of contamination. The accumulation of antibiotics may lead to the transfer or alternatively maintain the presence of resistance genes in natural microbial communities existing in epilithic biofilms. The objective of this study was to evaluate the pharmaceutical contamination levels and the presence of resistance genes in biofilms from a South Brazilian watershed. The Guaporé watershed exhibits a high diversity of land use, including agricultural and urban areas with differing levels of anthropogenic pressure. Seventeen sites along the Guaporé watershed were monitored. Biofilm samples were collected in two seasons (winter and summer), and the pharmaceutical concentration and quantity of resistance genes were analyzed. All monitored sites were contaminated with pharmaceuticals. Agricultural activities contribute through transferring pharmaceuticals derived from the application of animal waste to agricultural fields. The most contaminated site (pharmaceuticals and bacterial resistance genes) was located in an urban area exposed to high pressure. Decreases in the contamination of biofilms were also observed, exemplifying processes of natural attenuation in the watershed. The quality of the biofilms sampled throughout the watershed served as a useful tool to understand and monitor environmental pollution.

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Dried and milled eggplant fruit peel and calyces (PC) and mesocarp, placenta and core (Mes) were utilized as natural sources of valuable chemicals. Pectins were extracted with 0.1 M Na2CO3 (1 h; 23 °C). A high-power ultrasound (US) pretreatment (10 min net time; 12.76 W/cm2 power intensity) in 10:200 (g/mL) powder:water ratio led to the lowest solvent and energy consumptions after the subsequent 0.1 M Na2CO3 stirring, permitting the highest recoveries of uronic acid (UA) from PC and Mes (80.25 and 93.8 %, respectively). Homogalacturonans (>65 % w/w UA) of low degree of methylesterification, of acetylation, and 90,214-138,184 Da molecular weights with low polydispersity (≈1.32-1.40) were obtained. They included released ferulate (≈3.5 mg/100 g) esterified pectins. Antioxidants (caffeoylquinic acid, putrescine and spermidine derivatives, ß-carotene, lutein) gave additional technological value to their thickening effect as pectins protected tryptophan, tyrosine, alkyl side chains and sulfhydryl of skim milk proteins from UV-C photo-oxidation.

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The waste of fish resources constitutes a serious environmental problem that must be avoided. The valorisation of by-catch species and decreasing the discard rate constitute a more efficient and sustainable use of these marine biomasses. In this work, we characterize and propose different potential uses for Stromateus brasiliensis, another frequently discarded (≥ 90%) and poorly studied by-catch species captured in the South Atlantic Ocean (FAO 41) by trawler fishing fleets. Furthermore, in the case of this species, freezing and frozen storage of the whole fish is the only strategy currently employed for its exploitation. The results revealed that muscle from S. brasiliensis presented a high content of polyunsaturated fatty acids (20.34%) and that the concentrations of both total diacyl glyceryl ethers (2.41%) and heavy metals (Hg 0.038, Pb 0.006 and Cd 0.018 mg/kg) were below the established limits for safe human consumption. Likewise, the protein hydrolysates proved to be a good source of amino acids for human consumption or animal feeding. Minced muscle blocks could be made by a mechanical separation process of the flesh, and the composition of minced muscle did not differ much from that of the whole fish. Furthermore, this process allows the incorporation of cryoprotectants and antioxidants to extend the frozen shelf life of this fatty fish. An extraction process from mechanically mixed skin and bones yielded a good source of collagen that should not be neglected.

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BACKGROUND: Juglone is a naphthoquinone currently obtained by chemical synthesis with biological activities including antitumor activity. Additionally, juglone is present in the green husk of walnut, which suggests evaluating the effect of GH extracts on carcinogenic cell lines. RESULTS: Walnut green husk ethanolic extract was obtained as 169.1 mg juglone/100 g Green Husk and antioxidant activity (ORAC) of 44,920 µmol Trolox Equivalent/100 g DW Green Husk. At 1 µM juglone in HL-60 cell culture, green husk extract showed an antiproliferative effect, but pure juglone did not; under these conditions, normal fibroblast cells were not affected. A dose-dependent effect on mitochondrial membrane potential loss was observed. Apoptosis of HL-60 was detected at 10 µM juglone. Despite high ORAC values, neither purified juglone nor the extract showed protective effects on HL-60 cells under oxidative conditions. CONCLUSIONS: Green husk extract generates an antiproliferative effect in HL-60 cells, which is related to an induction of the early stages of apoptosis and a loss of mitochondrial membrane potential. The normal cells were not affected when juglone is present at concentrations of 1 µM, while at higher concentrations, there is loss of viability of both cancerous and healthy cells.

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BACKGROUND: Bacterial contamination remains the major problem in skin banks, even after antimicrobial treatment, and results in high rates of tissue discarding. This study aimed to analyze bacterial contamination in 32 human skin allografts from the skin bank of Dr. Roberto Corrêa Chem from the Hospital Complex Santa Casa de Misericórdia de Porto Alegre. These samples were already discarded due to microbial contamination. The identification of the bacteria isolated from skin allografts was performed by matrix assisted laser desorption ionization-time of flight. The antimicrobial susceptibility of the isolates to six different classes of antimicrobials was determined using the disk-diffusion agar method, and the evaluation of the inhibitory potential was determined by the minimal inhibitory concentration (50/90) of antimicrobials already used in the skin bank and those that most isolates were susceptible to. RESULTS: A total of 21 (65.6%) skin samples were contaminated with Gram-positive bacteria: 1 (4.7%) with Paenibacillus sp., 12 (61.9%) with Bacillus sp., 6 (28.5%) with Staphylococcus sp., and 2 (9.5%) with Bacillus sp. and Staphylococcus sp. Several resistance profiles, including multiresistance, were found among the isolates. Most of the isolates were susceptible to at least one of the antimicrobials used in the skin bank. All isolates were susceptible to amikacin, gentamicin, and tetracycline, which demonstrated the best inhibitory activities against the isolates and were considered as potential candidates for new antimicrobial treatments. CONCLUSIONS: Bacillus, Paenibacillus, and Staphylococcus were isolated from the skin allografts, thus demonstrating the predominance of Gram-positive bacteria contamination. Other factors not related to the resistance phenotype may also be involved in the persistence of bacterial isolates in the skin allografts after antibiotic treatment. Gentamicin, amikacin, and tetracycline can be considered as an option for a more effective treatment cocktail.

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Fisheries bycatch is a significant marine conservation issue as valuable fish are wasted and protected species harmed with potential negative ecological and socio-economic consequences. Even though there are indications that the small-scale handline fishery of the Galapagos Marine Reserve has a low selectivity, information on its bycatch has never been published. We used onboard monitoring and interview data to assess the bycatch of the Galapagos handline fishery by estimating the bycatch ratio, determining species compositions of landings and bycatch, identifying fishers' reasons for discarding certain individuals, and revealing historical trends in the bycatch ratio. The estimated bycatch ratio as a function of biomass of 0.40 and a diverse species composition of target catch and bycatch confirmed the low selectivity of this fishery. Most individuals were not landed for economic motivations, either because species (77.4%) or sizes (17.7%) are unmarketable or for regulatory reasons (5.9%). We found that bycatch contributes to growth overfishing of some target species because they are discarded or used as bait before reaching their first maturity. Moreover, over half of interviewees perceived a historical decrease in bycatch ratios that was explained by a diversification of the target catch due to the reduction in abundance of the traditionally most important target species. As some target species show signs of overfishing and to date there are no specific regulations for the finfish fishery species in place, we recommend the implementation of a series of management measures to protect critical life stages of overexploited species and to improve the selectivity of the Galapagos handline fishery.

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Business opportunities in the household waste sector in emerging economies still evolve around the activities of bulk collection and tipping with an open material balance. This research, conducted in Brazil, pursued the objective of shifting opportunities from tipping to reverse logistics in order to close the balance. To do this, it illustrated how specific knowledge of sorted waste composition and reverse logistics operations can be used to determine realistic temporal and quantitative landfill diversion targets in an emerging economy context. Experimentation constructed and confirmed the recycling trilogy that consists of source separation, collection infrastructure and reverse logistics. The study on source separation demonstrated the vital difference between raw and sorted waste compositions. Raw waste contained 70% biodegradable and 30% inert matter. Source separation produced 47% biodegradable, 20% inert and 33% mixed material. The study on collection infrastructure developed the necessary receiving facilities. The study on reverse logistics identified private operators capable of collecting and processing all separated inert items. Recycling activities for biodegradable material were scarce and erratic. Only farmers would take the material as animal feed. No composting initiatives existed. The management challenge was identified as stimulating these activities in order to complete the trilogy and divert the 47% source-separated biodegradable discards from the landfills.

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Discarding part of the catch is a common practice in worldwide fisheries. The make-up of the discards needs to be studied to evaluate the impact on the fishery as well as to correct data for stock assessment. It is also useful for the identification of commercially valuable species. 84 sets from 18 vessels in Tefé's purse seine fleet were observed over nine trips to the Lower Solimões River during the first semester of 1997. A questionnaire was filled out on board with the following information: date, hour of set, climate, name of the boat and skipper, fishing spot, gear used, size and mesh of the net, retained and discarded catch per species. A variation in the percentage of discards was observed over the months. Discards were higher than 20% until February, and from May onwards, but were less in March and April. Species were identified according to the following grouping: not discarded; occasionally discarded; and, always discarded. The mean fork length of discards was lower than that of retained fish for the following species: apapá; aracu; mapará; pacu; and, peixe-cachorro. However, there but was no significant difference in the sizes of discarded and retained aracu, branquinha, cubiu and sardinha. The fishermen had developed a net to select larger fish, the "escolhedeira", which allowed smaller fish to escape alive. Curimatã, matrinchã, pescada, cará and tucunaré were not discarded due to wide acceptance of these products in the local markets. Some discarded species like apapá, cubiu and branquinha that were often present in the catch have potential for alternative uses.

O descarte de parte da captura é pratica comum em pescarias de todo o mundo. A avaliação do impacto da pesca requer conhecer as características deste descarte e é útil para a identificação de itens utilizáveis comercialmente. Foram realizadas nove excursões de acompanhamento na região do baixo rio Solimões, em barcos de pesca comercial com redinha, durante o primeiro semestre de 1997, totalizando 18 barcos acompanhados, obtendo-se dados de 84 lances. A bordo foi preenchido um questionário com: data, hora do lance, clima, nome da embarcação e do encarregado, local de pesca, tipo de utensílio usado na captura, tamanho e malha da rede, captura por espécie em kg. Há variação no percentual descartado ao longo dos meses, havendo menor rejeição nos meses de março e abril. Descartes superiores a 20% ocorrem até fevereiro, voltando a repetir o padrão em maio. Itens não descartados, ocasionalmente descartados e sempre descartados foram determinados. Os tamanhos médios (comprimento furcal) das capturas descartadas foram inferiores aos da capturas conservadas para o apapá, aracu, mapará, pacu e peixe-cachorro. Porém, não apresentaram diferenças significativas para o aracu, a branquinha, o cubiu e a sardinha. Os pescadores utilizam uma rede para seleção do pescado grande, a "escolhedeira", permitindo aos peixes pequenos escaparem ainda com vida. Curimatã, matrinchã, pescada, cará e tucunaré grandes ou pequenos não são descartados devido à ampla aceitação destes produtos nos mercados locais. Algumas espécies rejeitadas, como apapá, cubiu, branquinha foram comuns nas capturas, apresentando potencial para uso alternativo.

RESUMEN

Discarding part of the catch is a common practice in worldwide fisheries. The make-up of the discards needs to be studied to evaluate the impact on the fishery as well as to correct data for stock assessment. It is also useful for the identification of commercially valuable species. 84 sets from 18 vessels in Tefé's purse seine fleet were observed over nine trips to the Lower Solimões River during the first semester of 1997. A questionnaire was filled out on board with the following information: date, hour of set, climate, name of the boat and skipper, fishing spot, gear used, size and mesh of the net, retained and discarded catch per species. A variation in the percentage of discards was observed over the months. Discards were higher than 20% until February, and from May onwards, but were less in March and April. Species were identified according to the following grouping: not discarded; occasionally discarded; and, always discarded. The mean fork length of discards was lower than that of retained fish for the following species: apapá; aracu; mapará; pacu; and, peixe-cachorro. However, there but was no significant difference in the sizes of discarded and retained aracu, branquinha, cubiu and sardinha. The fishermen had developed a net to select larger fish, the "escolhedeira", which allowed smaller fish to escape alive. Curimatã, matrinchã, pescada, cará and tucunaré were not discarded due to wide acceptance of these products in the local markets. Some discarded species like apapá, cubiu and branquinha that were often present in the catch have potential for alternative uses.

O descarte de parte da captura é pratica comum em pescarias de todo o mundo. A avaliação do impacto da pesca requer conhecer as características deste descarte e é útil para a identificação de itens utilizáveis comercialmente. Foram realizadas nove excursões de acompanhamento na região do baixo rio Solimões, em barcos de pesca comercial com redinha, durante o primeiro semestre de 1997, totalizando 18 barcos acompanhados, obtendo-se dados de 84 lances. A bordo foi preenchido um questionário com: data, hora do lance, clima, nome da embarcação e do encarregado, local de pesca, tipo de utensílio usado na captura, tamanho e malha da rede, captura por espécie em kg. Há variação no percentual descartado ao longo dos meses, havendo menor rejeição nos meses de março e abril. Descartes superiores a 20% ocorrem até fevereiro, voltando a repetir o padrão em maio. Itens não descartados, ocasionalmente descartados e sempre descartados foram determinados. Os tamanhos médios (comprimento furcal) das capturas descartadas foram inferiores aos da capturas conservadas para o apapá, aracu, mapará, pacu e peixe-cachorro. Porém, não apresentaram diferenças significativas para o aracu, a branquinha, o cubiu e a sardinha. Os pescadores utilizam uma rede para seleção do pescado grande, a "escolhedeira", permitindo aos peixes pequenos escaparem ainda com vida. Curimatã, matrinchã, pescada, cará e tucunaré grandes ou pequenos não são descartados devido à ampla aceitação destes produtos nos mercados locais. Algumas espécies rejeitadas, como apapá, cubiu, branquinha foram comuns nas capturas, apresentando potencial para uso alternativo.