RESUMEN

Insights from novel mechanistic paradigms in gene expression control have led to the development of new gene expression systems for bioproduction, control, and sensing applications. Coupled with a greater understanding of synthetic burden and modern creative biodesign approaches, contemporary bacterial gene expression tools and systems are emerging that permit fine-tuning of expression, enabling greater predictability and maximisation of specific productivity, while minimising deleterious effects upon cell viability. These advances have been achieved by using a plethora of regulatory tools, operating at all levels of the so-called 'central dogma' of molecular biology. In this review, we discuss these gene regulation tools in the context of their design, prototyping, integration into expression systems, and biotechnological application.

Asunto(s)

Bacterias/genética , Regulación Bacteriana de la Expresión Génica , Ingeniería Genética/métodos , Bacterias/crecimiento & desarrollo , Bacterias/metabolismo , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Sistema Libre de Células , Simulación por Computador , Retroalimentación Fisiológica/fisiología , Ensayos Analíticos de Alto Rendimiento/métodos , Iniciación de la Transcripción Genética