RESUMEN
Tympanic membrane perforations are due to common otologic problems. The current treatments to heal tympanic membrane perforation, such as myringoplasty, have some disadvantages, including the need for autologous grafting, which is rapidly absorbed by the organism before perforation recovery is complete. To improve the structural and functional tympanic membrane healing after surgery, we propose a new branch of artificial grafts. In this study, we report the development of artificial grafts using electrospun bioabsorbable polymers. Polymers such as poly (l-lactic acid) and poly (lactic-co-glycolic acid) acted as the scaffold for cell growth in a co-culture of fibroblasts and keratinocytes. This co-culture promoted the growth of an epithelial-equivalent tissue over the electrospun scaffold, which was used as an alternative graft in myringoplasty. The in vivo study was performed in Sprague Dawley rats. Ear endoscopy was performed 30days after surgery and showed that tympanic membrane perforations treated with artificial grafts healed naturally, completely and with the possibility of maintaining their actual functionality. In conclusion, our study described a new artificial graft created specifically to fulfill the requirements of perforated tympanic membrane healing processes, which are compatibility, proper durability and less intense side effects following myringoplasty.
Asunto(s)
Órganos Bioartificiales , Miringoplastia , Regeneración , Membrana Timpánica/fisiología , Membrana Timpánica/cirugía , Animales , Muerte Celular , Niño , Preescolar , Endoscopía , Humanos , Inmunohistoquímica , Lactante , Queratinas/metabolismo , Antígeno Ki-67/metabolismo , Masculino , Polímeros/química , Ratas Sprague-Dawley , Temperatura , Ingeniería de Tejidos , Andamios del Tejido/química , HumectabilidadRESUMEN
A composite is a material made of more than one component, and the bond between the components is on a scale larger than the atomic scale. The objective of the present study was to synthesize and perform the structural characterization and biological evaluation of a new biocomposite (BCO) based on a novel combination of an organic and an inorganic phase, for bone tissue engineering applications. The organic phase consisted of Wharton's jelly (WJ), which was obtained from embryonic tissue following a protocol developed by our laboratory. The inorganic phase consisted of bioceramic particles (BC), produced by sintering hydroxyapatite (HA) with ß- tricalcium phosphate (ß-TCP), and bioactive glass particles (BG). Each phase of the BCO was fully characterized by SEM, EDS, XRD, and FTIR. Biocompatibility was evaluated in vivo in the tibiae of Wistar rats (n = 40). Histological evaluation was performed at 0, 1, 7, 14, 30, and 60 days. XRD showed the phases corresponding to HA and ß-TCP, whereas diffractogram of BG showed it to have an amorphous structure. EDS showed mainly Si and Na, Ca, P in BG, and Ca and P in HA and ß-TCP. FTIR identified bonds between the organic and inorganic phases. From a mechanical viewpoint, the composite showed high flexural strength of 40.3 ± 0.8 MPa. The synthesized BCO exhibited adequate biocompatibility as shown by formation of lamellar type bone linked by BG and BC particles. The biomaterial presented here showed excellent mechanical and biocompatibility properties for its potential clinical use. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1034-1045, 2017.