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Artificial organelles (AOs) encapsulating enzymes are engineered to facilitate biocatalytic reactions for exerting therapeutic effects in various diseases. Exploiting the confinement effect, these catalytic properties exhibit significant enhancements without being influenced by the surrounding medium, enabling more efficient cascade reactions. In this study, we present a novel approach for synergistic tumor starvation therapy by developing multicomponent artificial organelles that combine enzymatic oncotherapy with chemotherapy. The construction process involves a microfluidic-based approach that enables the encapsulation of cationic cores containing doxorubicin (DOX), electrostatic adsorption of cascade enzymes, and surface assembly of the protective lipid membrane. Additionally, these multicomponent AOs possess multicompartment structures that enable the separation and sequential release of each component. By coencapsulating enzymes and chemotherapeutic agent DOX within AOs, we achieve enhanced enzymatic cascade reactions (ECR) and improved intrinsic permeability of DOX due to spatial confinement. Furthermore, exceptional therapeutic effects on 4T1 xenograft tumors are observed, demonstrating the feasibility of utilizing AOs as biomimetic implants in living organisms. This innovative approach that combines starvation therapy with chemotherapy using multicompartment AOs represents a promising paradigm in the field of precise cancer therapy.

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It is believed that nitrogen-fixing eukaryotes do not exist in nature, and constructing such eukaryotes is extremely challenging. Coale et al., however, have identified the first eukaryote capable of fixing nitrogen through a nitroplast organelle. Understanding the eukaryotic nitrogen-fixing machinery may advance the development of artificial nitrogen-fixing crops and industrial yeasts.

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Artificial organelles serve as functional counterparts to natural organelles, which are primarily employed to artificially replicate, restore, or enhance cellular functions. While most artificial organelles exhibit basic functions, we diverge from this norm by utilizing poly(ferrocenylmethylethylthiocarboxypropylsilane) microcapsules (PFC MCs) to construct multifunctional artificial organelles through water/oil interfacial self-assembly. Within these PFC MCs, enzymatic cascades are induced through active molecular exchange across the membrane to mimic the functions of enzymes in mitochondria. We harness the inherent redox properties of the PFC polymer, which forms the membrane, to facilitate in-situ redox reactions similar to those supported by the inner membrane of natural mitochondria. Subsequent studies have demonstrated the interaction between PFC MCs and living cell including extended lifespans within various cell types. We anticipate that functional PFC MCs have the potential to serve as innovative platforms for organelle mimics capable of executing specific cellular functions.

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In "cell-function editing", the combination of biological methods with artificial methods is a promising way to effectively implement functions that live cells do not originally possess. In the present symposium review, two approaches with methodology of building "artificial organelle" were implemented for editing cellular functions. One approach is the "membrane-bound artificial organelle", which is mainly created from polymeric nanocapsules that function in cells, and the other approach mimics the "membraneless organelle", which has recently gained immense interest in the field of cell biology. Furthermore, some examples of artificial cells are also described, which were constructed by utilizing artificial organelles. In this context, some recent progress has been observed in the author's research on the development of polyion complex (PIC) materials, in particular, PICsome-based nanoreactors, designer coacervates for protein sequestration, and yolk-shell PIC structures that are reminiscent of artificial cells. These technologies may contribute to effective "cell editing" or "cell renovation", which enables the edited cells to show higher performance at the target site in the human body, compared to the native cells.

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Biomineralization, a bio-organism controlled mineral formation process, plays an important role in linking biological organisms and mineral materials in nature. Inspired by biomineralization, biomimetic mineralization is used as a bridge tool to integrate biological organisms and functional materials together, which can be beneficial for the development of diversified functional organism-material hybrids. In this review, recent progresses on the techniques of biomimetic mineralization for organism-material combinations are summarized and discussed. Based upon these techniques, the preparations and applications of virus-, prokaryotes-, and eukaryotes-material hybrids have been presented and they demonstrate the great potentials in the fields of vaccine improvement, cell protection, energy production, environmental and biomedical treatments, etc. We suggest that more researches about functional organism and material combination with more biocompatible techniques should be developed to improve the design and applications of specific organism-material hybrids. These rationally designed organism-material hybrids will shed light on the production of "live materials" with more advanced functions in future. STATEMENT OF SIGNIFICANCE: This review summaries the recent attempts on improving biological organisms by their integrations with functional materials, which can be achieved by biomimetic mineralization as the combination tool. The integrated materials, as the artificial shells or organelles, confer diversified functions on the enclosed organisms. The successful constructions of various virus-, prokaryotes-, and eukaryotes-material hybrids have demonstrated the great potentials of the material incorporation strategy in vaccine development, cancer treatment, biological photosynthesis and environment protection etc. The suggested challenges and perspectives indicate more inspirations for the future development of organism-material hybrids.

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Artificial organelles (AOs) are typical microcompartments with intracellular biocatalytic activity aimed to replace missing or lost cellular functions. Currently, liposomes or polymersomes are popular microcompartments to build AOs by embedding channel proteins in their hydrophobic domain and entrapping natural enzymes in their cavity. Herein, a new microcompartment is established by using monolayer cross-linked zwitterionic vesicles (cZVs) with a carboxylic acid saturated cavity. The monolayer structure endows the cZVs with intrinsic permeability; the cavity supplies the cZVs ability of in situ synthesis of artificial enzymes, and the pH-dependent charge-change property makes it possible to overcome the biological barriers. Typically, nanozymes of CeO2 and Pt NPs were synthesized in the cZVs to mimic peroxisome. In vitro experiments confirmed that the resulting artificial peroxisome (AP) could resist protein adsorption, endocytose efficiently, and escape from the lysosome. In vivo experiments demonstrated that the APs held a good therapeutic effect in ROS-induced ear-inflammation.

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For energy supply to biomimetic constructs, a complex chemical energy-driven ATP-generating artificial system was built. The system was assembled with bottom-up detergent-mediated reconstitution of an ATP synthase and a terminal oxidase into two types of novel nanocontainers, built from either graft copolymer membranes or from hybrid graft copolymer/lipid membranes. The versatility and biocompatibility of the proposed nanocontainers was demonstrated through convenient system assembly and through high retained activity of both membrane-embedded enzymes. In the future, the nanocontainers might be used as a platform for the functional reconstitution of other complex membrane proteins and could considerably expedite the design of nanoreactors, biosensors, and artificial organelles.

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In this review, we briefly introduce our efforts to reconstruct cellular life processes by mimicking natural systems and the applications of these systems to energy and environmental problems. Functional units of in vitro cellular life processes are based on the fabrication of artificial organelles using protein-incorporated polymersomes and the creation of bioreactors. This concept of an artificial organelle originates from the first synthesis of poly(siloxane)-poly(alkyloxazoline) block copolymers three decades ago and the first demonstration of protein activity in the polymer membrane a decade ago. The increased value of biomimetic polymers results from many research efforts to find new applications such as functionally active membranes and a biochemical-producing polymersome. At the same time, foam research has advanced to the point that biomolecules can be efficiently produced in the aqueous channels of foam. Ongoing research includes replication of complex biological processes, such as an artificial Calvin cycle for application in biofuel and specialty chemical production, and carbon dioxide sequestration. We believe that the development of optimally designed biomimetic polymers and stable/biocompatible bioreactors would contribute to the realization of the benefits of biomimetic systems. Thus, this paper seeks to review previous research efforts, examine current knowledge/key technical parameters, and identify technical challenges ahead.