RESUMEN

The objective of this study was to evaluate the synergistic effect of eggshell-derived nanohydroxyapatite (EnHA) and carboxymethyl chitosan (CMC) in remineralizing artificially induced dentinal lesions. EnHA and CMC were synthesized using simple chemical processes and characterized using FTIR, XRD, HRSEM-EDX, TEM, DLS and TGA/DTA analyses. A total of 64 pre-demineralized coronal dentin specimens were randomly subjected to following treatments (n = 16):artificial saliva (AS), EnHA, CMC, and EnHA-CMC, followed by pH cycling for 7 days. HRSEM-EDX, Vickers-indenter, and micro-Raman analyses were used to assess surface-topography, microhardness, and chemical analysis, respectively. All tested materials demonstrated non-cytotoxicity when assessed on hDPSCs using MTT assay. FTIR, XRD and thermal analyses confirmed the characteristics of both EnHA and CMC. EnHA showed irregular rod-shaped nanoparticles (30-70 nm) with the presence of Ca,P,Na, and Mg ions. Dentin treated with EnHA-CMC exhibited complete tubular occlusion and highest microhardness whereas the AS group revealed the least mineral deposits (p < 0.05). No significant differences were observed between EnHA and CMC groups (p > 0.05). In addition, molecular conformation analysis revealed peak intensities in collagen's polypeptide chains in dentin treated with CMC and EnHA-CMC, whereas other groups showed poor collagen stability. The results highlighted that EnHA-CMC aided in rapid and effective biomineralization, suggesting its potential as a therapeutic solution for treating dentin caries.

Asunto(s)

RESUMEN

OBJECTIVES: The purpose of the study was to report a step-by-step process of creating artificial caries typodont teeth and to determine the perception and efficacy of their use in preclinical operative training. METHODS: Artificial caries material comprised of commercially available hide glue and chocolate powder for more realistic coloring was embedded into the distolingual of #9 ModuPRO plastic typodont teeth. First-year dental students having no clinical experience in excavating Class III cavity preparations were divided into two groups. Group BA prepared conventional typodont teeth (CTT) first, then artificial caries typodont teeth. Group AB prepared the ACT first, then CTT. The preps were scored employing a rubric used in the operative dentistry course class. A feedback questionnaire was conducted to rate students' satisfaction regarding the use of ACT and CTT. The Mann-Whitney U-test was used to compare the scores between groups ACT-CTT and CTT-ACT and the Chi-Square test was used to evaluate the positive and negative questionnaire responses. RESULTS: The two groups showed no significant difference in grades and no significant changes in their scores regardless of which order they prepped the teeth (P > 0.05). The questionnaire heavily favored the use of artificial caries typodont teeth (P < 0.05). CONCLUSIONS: The artificial caries typodont teeth protocol described in this study was feasible when implemented at the preclinical laboratory instruction level with positive questionnaire feedback from dental students.

RESUMEN

BACKGROUND: This study compared natural root caries lesions with artificial root caries lesions prepared with one of the two demineralising solutions. METHODS: Twelve natural root caries lesions on upper incisors and 24 artificial root lesions were prepared on sound root surfaces using 50 mM acetic acid, 1.5 mM CaCl2 , 0.9 mM KH2 PO4 at pH 5.0 or 80 mL/L Noverite K-702 polyacrylate solution, 500 mg/L hydroxyapatite, 0.1 mol/L lactic acid at pH 4.8 (n = 12/group) for 96 hours. Lesions were scanned using micro-CT. Inciso-gingival oriented images were analysed and mineral density calculated at 7.5 µm increments from the surface to 225-µm deep. Sectioned lesions were analysed by Knoop microhardness up to 250 µm from the lesion surface. Data were analysed by the Dunn's test with Bonferroni correction. RESULTS: Natural and artificial lesion mean mineral densities were not statistically different (P > 0.05). Mineral density from the surface to 75 µm was greater in natural lesions and from 150 to 225 µm was greater in artificial lesions (P < 0.05). Microhardness values were statistically higher in artificial lesions (P < 0.05); no difference was found among artificial lesions produced by the two solutions (P > 0.05). Mineral density and microhardness of natural and artificial root caries are different from each other. A greater mineralized surface layer existed on natural lesions.

Asunto(s)

RESUMEN

BACKGROUND: Professionally applied topical fluoride preparations have been commonly used and have proven to prevent dental decay. Alternative preparations that provide further benefits may be of interest to investigate. This study aimed to investigate the effect of experimental nano silver fluoride (NSF) formulation compared to commercial sodium fluoride varnish (FV) on prevention of in vitro demineralization of initially sound enamel in primary teeth. METHODS: Forty-eight extracted/exfoliated sound molars were sectioned buccolingually into 96 specimens then assigned randomly into two equal groups. Each group was further subdivided into two equal subgroups (Ia: NSF, IIa: FV, Ib and IIb as negative controls). The test materials were applied, then all the specimens were subjected to a demineralization pH cycling model for 7 days. Specimens were examined for surface microhardness using Vickers microhardness device and lesion depth was evaluated by polarized light microscope using image J 1.46r software. Data were analyzed using paired t-test, independent t-test, and Mann Whitney U test. RESULTS: The test materials were significantly superior to their negative controls, (P < 0.001) and comparable to each other, (P > 0.05) regarding microhardness and lesion depth. In comparison to FV, NSF showed lower yet statistically insignificant percent increase in microhardness and decrease in lesion depth, (P = 0.81, 0.86, respectively). Qualitative evaluation revealed that both agents reduced the lesion depth formation. CONCLUSION: NSF showed similar effect to that of FV in limiting in vitro enamel demineralization caused by acidic challenge. Hence, it could be regarded as a promising alternative preventive agent in primary teeth.

Asunto(s)

RESUMEN

This study aimed to evaluate the Cirrus high-definition (HD) spectral-domain optical coherence tomography (SD-OCT) for the remineralization of artificial enamel caries and to compare it with the comparison surface microhardness (SMH) analysis. Artificial caries lesions were produced on forty human enamel samples. Then, three different remineralization agents containing casein phosphopeptide amorphous calcium phosphate; casein phosphopeptide amorphous calcium fluoride phosphate; calcium glycerophosphate, magnesium chloride, and xylitol; and remineralization solution (control) were applied with pH cycling for six days. The optical depth of backscattered light and microhardness of enamel were measured using SD-OCT and SMH. All remineralization agents were significantly efficient in reducing optical lesion depth on enamels (p1=0.001, p2=0.002, p3=0.006, p4=0.025), and in increasing the SMH of enamels (p1-3=0.005, p4=0.017). However, the optical lesion depths of the enamel showed no correlation with the SMH in the groups. In conclusion, demineralization and remineralization of artificial lesions can be assessed with both SD-OCT and SMH.

Asunto(s)

RESUMEN

OBJECTIVE: To assess the ultrastructural change of demineralized dentin collagen during calcium phosphate polymer-induced liquid precursor (Ca/P-PILP) mediated remineralization process and to evaluate the biomimetic remineralization potential of high concentration Ca/P-PILP at demineralized artificial caries dentin lesion, additionally to investigate the bond interfacial integrity as well as the bonding strength of the biomimetic remineralized artificial caries dentin lesion. METHODS: Demineralized dentin collagen of 5 µm thick was biomimetically remineralized with low, medium concentration Ca/P-PILP for 10 days and high concentration Ca/P-PILP for 10, 15, 20 days. Artificial caries dentin lesion at a thickness of 150 ± 50 µm was biomimetically remineralized with high concentration Ca/P-PILP for 20 days. The biomimetic remineralization of demineralized dentin collagen was observed by scanning electron microscopy (SEM). The biomimetic remineralization intensity and depth of artificial caries dentin lesion was assessed by Electron Probe Micro Analyzer (EPMA). The bonding interfacial integrity between remineralized artificial caries dentin and composite resin was observed by Swept-source optical coherence tomography (SS-OCT) and the bonding strength of remineralized artificial caries dentin was evaluated by micro-tensile bond strength analysis (µTBS). RESULTS: Solely PAA-PASP solution and solely saturated Ca/P solution can't achieve dentin collagen remineralization. Increased concentration of Ca/P-PILP and prolonged remineralization time can enhance the biomimetic remineralization intensity of demineralized dentin collagen. After treating with high concentration Ca/P-PILP, a 150 ± 50 µm thick layer of demineralized artificial caries dentin lesion was not fully remineralized, and the biomimetic remineralization intensity reached up to 88.0%. Furthermore, a better bonding interfacial integrity with less microgap and increased bond strength at both baseline level and aging level were observed when artificial caries dentin lesion was biomimetically remineralized with high concentration Ca/P-PILP. SIGNIFICANCE: Biomimetic remineralization of demineralized caries dentin lesion promotes its clinical properties for resin composited adhesive restoration.

Asunto(s)

RESUMEN

OBJECTIVES: To compare the ability of resin-modified glass ionomer (RMGI) and bioactive cements to prevent root dentin demineralization. MATERIALS AND METHODS: Fifty molars were prepared at the cementum-enamel junction (n = 10) and restored with three bioactive cements (Activa Bioactive Cement, ACT; Ceramir Crown and Bridge, CER; and Theracem, THE), a self-adhesive resin cement (Rely X Unicem 2, UNI), and a RMGI cement (Rely X Luting Plus, LUT). Specimens were cycled for 30 days between a demineralization solution (pH = 4) composed of 0.1 M lactic acid and 3 mM Ca3 (PO4 )2 for 4 hours and a remineralization solution (pH = 7.0) composed of 1.5 mM Ca, 0.9 mM P, and 20 mM Tris(hydroxymethyl)-aminomethane for 20 hours. Specimens were sectioned to 100 µm and evaluated with polarized light microscopy. A line was drawn parallel with the zone of demineralization for each tooth. The areas of "inhibition" (external to the line) were measured as positive values and "wall lesions" (pulpal to the line) were measured as negative areas. RESULTS: Significant differences were found between materials for "inhibition/wall lesion" areas in root dentin (P < .001) and ranked as (µm2 , mean ± SD): LUT (7700 ± 2500) > CER (3800 ± 1900), THE (2100 ± 2600), and ACT (1400 ± 700) > UNI (-2000 ± 1700). CONCLUSIONS: Bioactive cements showed net areas of demineralization inhibition albeit at a lower level than a reference RMGI cement. CLINICAL SIGNIFICANCE: RMGI or bioactive cements may be indicated for patients at risk of secondary caries around crown margins.

Asunto(s)

RESUMEN

The aim of this study was to evaluate the feasibility of applying the polymer-induced liquid-precursor (PILP) method to enhance silver diamine fluoride (SDF) therapy. One hundred forty micrometer deep artificial caries lesions were treated with (A) 38% SDF solution and (B) 38% SDF containing poly-L-aspartic acid (pASP). Changes in the nanomechanical profile across the lesion were evaluated. Hydrated artificial lesions had a low reduced elastic modulus (0.3 GPa) and nanohardness (0.02 GPa) region extending about 100 µm into the lesion, with a gradual linear increase to about 168 µm where the values plateaued to around 18 GPa/1.0 GPa. Topical application of SDF resulted in significantly recovered properties (p<0.001). SDF containing pASP resulted in greater nanomechanical properties compared to SDF alone, showing similar sloped regions up to 96 µm, then SDF alone dropped while SDF containing pASP continued at a modest slope until reaching normal at 144 µm. This nanoindentation study shows enhanced SDF therapy using the PILP method.

Asunto(s)

RESUMEN

Introduction and aim Dental caries is the global burden worldwide and has a negative effect on the quality of life. Restorative materials in pediatric dentistry have shown satisfactory properties; however, the highest failures are still reported due to the occurrence of secondary caries. The article aims to assess the effectiveness of pre-treatment with nano-silver fluoride (NSF) in inhibiting secondary caries at the tooth restoration interface. Materials and methods Forty tooth specimens were prepared from 20 newly extracted human premolar teeth by sectioning the tooth mesiodistally. Class V cavities were prepared on each specimen at enamel dentin junction. Specimens were randomly distributed into four groups (n=10): 1) glass ionomer cement (GIC) restoration, 2) composite restoration 3) NSF pre-treatment + GIC restoration, 4) NSF pre-treatment + composite restoration. After sterilization, specimens were subjected to artificial caries formation by pH cycling method for 14 days. Specimens were sectioned and mounted to evaluate the demineralization by using the Vickers microhardness test. Outer lesion depth was measured at the tooth restoration interface on digital radiographs. Data was analyzed using Student unpaired t-test, one-way ANOVA, and Tukey honestly significant difference (HSD) post hoc test. Results The mean microhardness value of pre-treated GIC and composite group with NSF was more than the non-treated NSF group, with a significant difference at the enamel, indicating lesser demineralization. Outer lesion depth was lesser in the pre-treated group showing better tooth restoration integrity with a statistically significant difference between the groups. Conclusion Pre-treatment with NSF is beneficial in increasing the resistance of GIC and composite resin restoration to secondary caries formation.

RESUMEN

BACKGROUND: This study investigated the remineralisation effect of bioactive glass on artificial dentine caries. METHODS: Dentine disks with artificial caries were treated with bioactive glass (group BAG), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) (group CPP-ACP), sodium fluoride glycerol (group F) or deionized water (group W). All disks were subjected to pH cycling for 28 days subsequently. The topography, microhardness and remineralisation depth of the dentine carious lesion were assessed by atomic force microscopy (AFM), microhardness testing and confocal laser scanning microscope (CLSM), respectively. RESULTS: AFM images indicated mineral depositions on the surface of the carious lesion in group BAG. The changes of Vickers hardness number (ΔVHN, mean ± SD) after pH cycling were 9.67 ± 3.60, 6.06 ± 3.83, 5.00 ± 2.19 and - 1.90 ± 2.09 (p < 0.001) in group BAG, group CPP-ACP, group F and group W, respectively. The remineralisation depth (mean ± SD) of the carious lesion in group BAG, group CPP-ACP, group F and group W were 165 ± 11 µm, 111 ± 11 µm, 75 ± 6 µm and 0 µm (p < 0.001), respectively. CONCLUSION: Bioactive glass possessed a promising remineralisation effect on artificial dentine caries and could be a therapeutic choice for caries management.

Asunto(s)

RESUMEN

PURPOSE: The purpose of the study was to understand the mineral loss in primary and permanent enamel samples and an attempt is made to standardize the process of chemical demineralization to generate more meaningful results in research studies involving the remineralization of demineralized samples. MATERIALS AND METHODS: Due to variability among enamel samples theoretically, it is impossible to standardize demineralization by running time-based chemical demineralization cycle without frequent monitoring. Instead of carrying out demineralization cycles for a fixed duration of time, we quantified the mineral loss 24 hourly using the International Caries Detection and Assessment System (ICDAS) and Quantitative Light-induced Fluorescence System (QLF™). Twenty primary and permanent enamel samples were subjected to demineralization, and ICDAS and QLF™ evaluation were done at 0, 24, 48, 72, 96, 120, 144, and 168 h of demineralization. RESULTS: The first visual change in permanent enamel is appreciated at 24 h (ICDAS II code1, QLF™ code1 -16.353 - ΔF) of demineralization, at 48 h (ICDAS II code2, QLF™ code2, -24.515 - ΔF), there was localized white spot lesion in permanent enamel and remained until 96 h (ICDAS II code 2, QLF™ code 2, -25.739 - ΔF) of demineralization. In primary samples, distinct visual change was seen at 24 h (ICDAS II code2, QLF™ code2, -19.431 - ΔF), and at 48 h clinically, there was a distinct visual change, but optically mild enamel breakdown was appreciated (ICADSII code 2 QLF™ code3, -27.201 - ΔF), which remained constant till 120 h of demineralization (ICDAS II code2 QLF™ code3 -37.645 - ΔF). CONCLUSION: Different samples demineralize at different rates. The demineralization in primary samples was 1.25 times higher than permanent samples. Recommendation: due to inherent variability in the samples continuous monitoring of the demineralization process on a 24 hourly basis is required to standardize the process.

Asunto(s)

RESUMEN

PURPOSE: To evaluate the remineralisation effect of an experimental nano silver fluoride (NSF) formulation using an in vitro remineralisation model, compared with silver diamine fluoride (SDF) and sodium fluoride varnish (NaF) in current clinical use. MATERIALS AND METHODS: 45 sound human third molars were sectioned in buccolingual and mesiodistal direction and 180 enamel specimens were divided into four groups (NSF; SDF; NaF; Control). Early caries-like lesions were artificially created. Remineralisation agents were applied with a microbrush for 2 min in NSF and SDF group; NaF varnish were applied in a thin layer and then all specimens were stored in a moist environment at 37°C for 24 h. Each group was subjected to a pH cycling model for 7 days. The Vickers microhardness values (VHN) of specimens were compared before treatment, after demineralisation and after remineralisation. In addition, scanning electron microscope (SEM) images were investigated to compare morphological changes on the surfaces. Kruskal-Wallis and Mann-Whitney U tests were used for statistical analysis to compare the groups at p < 0.05. RESULTS: All remineralisation agents were found to be statistically significant for the rehardening of the demineralised enamel specimens (p < 0.001). There was a statistically significant difference between the all groups after remineralisation (p < 0.001). The VHN values for postdemineralisation were determined as, respectively, NaF (229.96) > SDF (222.96) > NSF (191.36) > Control (175.80). SEM images have also supported the microhardness values. CONCLUSIONS: NSF was not found to be effective as sodium fluoride varnish and SDF on artificial enamel caries lesions. Additional investigation is needed to recommend it as an alternative agent to routine fluoride treatments.

Asunto(s)

RESUMEN

Objective: The objective of this study was to compare the remineralizing effect of sodium fluoride (NaF) mouth rinse or NaF gel as an adjunct to NaF dentifrice on incipient caries-like lesions in an in situ cross-over design study, with three sessions of 30 days each. Materials and methods: Orthodontic brackets with artificial demineralized enamel slabs were attached to the upper first molars of 12 participants. A set of 3 test specimens from the same tooth was randomly assigned to each participant and allocated into three 30-day sessions: 1) brushing with 0.22% NaF dentifrice 2 times/day (F dentifrice), 2) brushing with 0.22% NaF dentifrice 2 times/day+ rinsing with 0.05% NaF before bedtime (F mouth rinse), 3) brushing with 0.22% NaF dentifrice 2 times/day + brushing with 1.1% NaF gel before bedtime (F brush-on gel). The mineral gain and lesion depth of the specimens were evaluated by micro-computed tomography. Results: The mean mineral gain from the NaF mouth rinse and the NaF brush-on gel was similar, but greater than that from the NaF dentifrice (p < .05). The NaF brush-on gel yielded the greatest mean depth of remineralization (168 µm), followed by the NaF mouth rinse (144 µm). Both depths were significantly greater than that of the NaF dentifrice (84 µm) (p < .05). Conclusions: Both 0.05% NaF mouth rinse and 1.1% NaF brush-on gel, used at bedtime, increased incipient caries-like lesion remineralization in situ in combination with brushing with NaF dentifrice twice a day.

Asunto(s)

RESUMEN

OBJECTIVES: Initial dental caries often occurs in clinic. Reduction of cariogenic bacteria and promotion of remineralization are effective ways to control them. This study was to develop bifunctional anticaries peptides with antibacterial and remineralizing properties. METHODS: We designed peptides TDH19, TNH19, and TVH19 and selected one through comparing their minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) against Streptococcus mutans and their reaction on mineralization. Then the bifunction of the selected peptide was studied through: (a) effects on S. mutans biofilm, (b) remineralizing effects on initial lesions and (c) stability in saliva and cytocompatibility to human oral keratinocytes (HOKs). RESULTS: TVH19 showed the lowest MIC and MBC and a better mineralizing ability. It inhibited new biofilm formation and reduced the viability of old biofilm (p < 0.05). Treating initial caries with TVH19 led to greater recovery of surface microhardness, shallower lesion depth, and higher mineral content (p < 0.05). No significant differences were observed between TVH19 and NaF samples (p > 0.05). TVH19 was stable in saliva and had little effect on HOKs. CONCLUSIONS: The novel bifunctional anticaries peptide TVH19 was developed with remarkable antibacterial activity and the potential to enhance remineralization of initial caries.

Asunto(s)

RESUMEN

OBJECTIVES: This study aimed to develop a method to induce carious lesions in the pulpal floor dentin of a class II cavity preparation, and to determine the effects of this carious lesion on the biomechanical behavior of the dental composite restoration. METHODS: The pulpal floor dentin of class I cavities in sound third molars were demineralised with acetic acid for 35days followed by a 7-day exposure to pooled human saliva biofilm and demineralization was verified by micro-CT. Subsequently, the proximal walls were removed forming a class II cavity and the caries lesion was left intact or was completely removed prior to restoration with a bulk-fill dental composite (n=10). Cuspal deflection was assessed by strain-gauge and micro-CT imaging. The presence of enamel cracks was assessed by transillumination before and after restoration, and again after 1,200,000 cycles of mechanical fatigue in a chewing simulator. Finally, resistance to fracture by axial compressive loading and failure mode was determined. Data were analyzed by 2-way repeated measures ANOVA, Fisher's exact test, and t-test (α=0.05). RESULTS: The presence of carious lesions had no significant effect upon cuspal deflection, formation of enamel cracks, and fracture strength of the dental composite restorations. The restorative procedure increased the number of enamel cracks, which was not affected by mechanical cycling. SIGNIFICANCE: Maintaining carious lesions does not affect the biomechanical behavior of class II restorations performed with bulk-fill dental composite.

Asunto(s)

RESUMEN

BACKGROUND/PURPOSE: Remineralization efficacy for early caries lesion may change when fluoride varnish (FV) is applied directly or indirectly to the lesion. This in vitro study compared direct and indirect remineralization efficacies of FV on artificial caries lesions and evaluated acid-resistance of lesion remineralized by FV and artificial saliva. MATERIALS AND METHODS: One hundred and twenty-six bovine demineralized specimens were allocated to four varnish groups (Duraphat®, EnamelPro®, MI™, and ClinproWhite™, n = 28 each) and a negative-control group (n = 14). Half of specimens from each varnish group had the FV applied and the other specimens didn't. The specimens treated and not treated with the FV were immersed together in 20 mL of artificial saliva at 37 °C for 24 h. Then the applied FV was removed carefully from the specimen, and immersion process was continued in fresh artificial saliva for 48 h. The negative-control group was immersed in artificial saliva for same time as in varnish groups. The acid resistance of remineralized specimens from varnish groups was compared to negative-control group. Vickers microhardness number (VHN) was measured to evaluate re-demineralization effect. RESULTS: The ΔVHN was significantly higher for indirect remineralization (134.4 ±â€¯31.5, mean ±â€¯SD) than for direct remineralization (66.8 ±â€¯27.9). All varnish groups showed significant differences between the direct and indirect application methods. The acid resistance of remineralized specimens was higher in the all FV groups than in the negative-control. CONCLUSION: This in vitro study confirmed that the remineralization effect of fluoride varnishes would be higher in the vicinity than the underneath of the varnish treated surface.

RESUMEN

OBJECTIVES: To investigate the remineralizing effect of 38% silver diamine fluoride (SDF) application on enamel artificial caries in adjunct to 1000ppm fluoride toothpaste compared with fluoride toothpaste alone by analyzing the mineral density, depth of remineralization, and remineralization percentage of the lesions. METHODS: Eighteen artificial caries slabs were created from the proximal surfaces of nine chemically demineralized premolars. The slabs were scanned by Micro-Computed Tomography (Micro-CT) to determine the baseline mineral density of the initial lesions and randomly allocated into 2 groups. The test group was applied with 38% SDF in adjunct to fluoride toothpaste and the control group was treated with fluoride toothpaste alone. The specimens underwent bacterial pH-cycling for 5 d and were re-evaluated using Micro-CT. The pre-treatment and post-treatment mineral densities were plotted and the areas under the curves were used to calculate the remineralization percentage of both groups. RESULTS: Mineral density significantly increased in both groups after pH-cycling (p<0.05) although to different depths (control group=260µm, test group=300µm). The test group demonstrated a significantly higher mineral density to a depth of 120µm and higher remineralization percentage (p<0.05) compared with the control group. CONCLUSION: The adjunctive use of 38% SDF enhances the remineralization of initial carious lesions based on mineral density, depth, and remineralization percentage compared with the use of 1000ppm fluoride toothpaste alone. SDF might be used as an adjunct to fluoride toothpaste to remineralize incipient caries lesions on smooth tooth surfaces. CLINICAL SIGNIFICANCE: In non-compliant patients, the application of 38% SDF might be used as an adjunct to fluoride toothpaste, to remineralize incipient caries lesions of permanent teeth where esthetics is not a concern.

Asunto(s)

RESUMEN

BACKGROUND: Different formulations of dentifrices exist in the market. Usually, single toothpaste is used by all family members including children. There is a big concern of fluoride ingestion with the toothpaste containing high fluoride content in children. Recently, new toothpaste (including toothpaste) with remineralization potential without fluoride content has been formulated. AIM: There is an urgent need to compare remineralization potential of this new formulation with the exiting dentifrices. Therefore, the present study has been undertaken to assess and compare the remineralization potential of three dentifrices with different compositions on artificially induced carious lesions in vitro by using scanning electron microscopy and polarised light microscopy. MATERIALS AND METHODS: The present in vitro study was conducted on 21 healthy extracted primary central incisor teeth surfaces, which were divided into three groups and were treated by three different dentifrices. Artificial demineralization was followed by remineralization using dentifrice slurry as per the group distribution. All the samples were studied for remineralization by using scanning electron microscopy and polarised light microscopy. Data were analysed using SPSS version 11 software. RESULTS: A significant difference was found between the remineralization potential of incudent toothpaste and other toothpaste groups based on the analysis of polarised light microscopy and stereomicroscope. The remineralizing ability of incudent toothpaste for artificial enamel lesions was found to be significantly higher than that of Colgate® and Crest toothpaste. CONCLUSIONS: The limitations of this study include, being a short term study, low sample size and in vitro experiment. incudent toothpaste has exhibited a higher remineralizing potential as compared to fluoride based toothpaste in our study.

RESUMEN

The aim of this systematic review is to characterize and discuss key methodological aspects of in vitro biofilm models for caries-related research and to verify the reproducibility and dose-response of models considering the response to anti-caries and/or antimicrobial substances. Inclusion criteria were divided into Part I (PI): an in vitro biofilm model that produces a cariogenic biofilm and/or caries-like lesions and allows pH fluctuations; and Part II (PII): models showing an effect of anti-caries and/or antimicrobial substances. Within PI, 72.9% consisted of dynamic biofilm models, while 27.1% consisted of batch models. Within PII, 75.5% corresponded to dynamic models, whereas 24.5% corresponded to batch models. Respectively, 20.4 and 14.3% of the studies reported dose-response validations and reproducibility, and 32.7% were classified as having a high risk of bias. Several in vitro biofilm models are available for caries-related research; however, most models lack validation by dose-response and reproducibility experiments for each proposed protocol.

Asunto(s)

RESUMEN

FCP-COMPLEX is a newly-developed solution that contains fluoride, calcium, and phosphoric acid. It has the potential to reinforce caries-affected dentin. The purpose of this study was to evaluate the effect of FCP-COMPLEX on the morphology of bovine artificial caries-affected dentin (ACAD). FCP-COMPLEX, 2% sodium fluoride solution, and distilled water were applied to bovine ACAD and the effect on acid-induced damage was observed. Scanning electron microscopy was used to evaluate morphology after acid application. Energy dispersive X-ray spectroscopy was used for mapping and quantitative analyses of the dentin surface, and for line analysis of dentin-adhesive interface. The FCP-COMPLEX precipitated on the dentin surface and fluoride, calcium, and phosphorus were detected at a higher level in the FCP-COMPLEX group. The area of acid damage in caries-affected dentin was reduced by FCP-COMPLEX. In conclusion, FCP-COMPLEX significantly increased the deposition of fluoride onto bovine ACAD and inhibited demineralization.

Asunto(s)