RESUMEN
Salvia mellifera, native to California, Baja California, and Mexico, is a medicinal herb traditionally used to relieve pain, body aches, including chronic pain. A detailed phytochemical investigation of aerial parts of S. mellifera was accomplished to find species-specific markers and to differentiate the closely related, often (un)intentionally substituted with S. apiana. A total of 22 metabolites, including flavonoids (1-14), triterpenoids (15-18), diterpenoids (19-21), and phenylpropanoid (22), were isolated and characterized thoroughly. Among the isolates, eupatorin 3'-O-glucopyranoside (1) was identified as undescribed phytochemical and detailed structure elucidation was achieved through extensive NMR and mass spectral data analysis.
Asunto(s)
Salvia , Salvia/química , Glucósidos/análisis , México , Flavonoides/química , Componentes Aéreos de las Plantas/química , Fitoquímicos/análisisRESUMEN
Organic extracts from leaves plus branches plus inflorescences of Ageratum fastigiatum (Gardner) R. M. King & H. Rob., Asteraceae, were fractionated through classic chromatography. The steroids stigmasterol, chondrillasterol and campesterol were isolated from hexane extract. The triterpenes lupeol, taraxasterol, α-amyrin, β-amyrin, pseudotaraxasterol, lupeol acetate and α-amyrin acetate were isolated from ethyl acetate extract. Steroids and triterpenes were identified by GC-MS. The coumarin ayapin was isolated from ethanol extract and identified by NMR. Essential oils of the fresh leaves and fresh inflorescences were obtained by hydrodistillation and analyzed for GC-MS. The main components in both essential oils were α-pinene, limonene and germacrene D.
RESUMEN
Artemisia herba-alba Asso., Asteraceae, is widely used in Morrocan folk medicine for the treatment of different health disorders. However, no scientific or medical studies were carried out to assess the cytotoxicity of A. herba-alba essential oil against cancer cell lines. In this study, eighteen volatile compounds were identified by GC-MS analysis of the essential oil obtained from the plant's aerial parts. The main volatile constituent in A. herba-alba was found to be a monoterpene, Verbenol, contributing to about 22 percent of the total volatile components. The essential oil showed significant antiproliferative activity against the acute lymphoblastic leukaemia (CEM) cell line, with 3 µg/mL as IC50 value. The anticancer bioactivity of Moroccan A. herba-alba essential oil is described here for the first time.