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Antimicrobial resistance poses a serious threat to human health. Hospital wastewater system (HWS) is an important source of antibiotic resistance genes (ARGs). The risk of ARGs in HWS is still an under-researched area. In this study, we collected publicly metagenomic datasets of 71 hospital wastewater samples from 18 hospitals in 13 cities. A total of 9838 contigs were identified to carry 383 unique ARGs across all samples, of which 2946 contigs were plasmid-like sequences. Concurrently, the primary hosts of ARGs within HWS were found to be Escherichia coli and Klebsiella pneumoniae. To further evaluate the risk of each ARG subtype, we proposed a risk assessment framework based on the importance of corresponding antibiotics as defined by the WHO and three other indicators - ARG abundance (A), mobility (M), and host pathogenicity (P). Ninety ARGs were identified as R1 ARGs having high-risk scores, which meant having a high abundance, high mobility, and carried by pathogens in HWS. Furthermore, 25% to 49% of genomes from critically important pathogens accessed from NCBI carried R1 ARGs. A significantly higher number of R1 ARGs was carried by pathogens in the effluents of municipal wastewater treatment plants from NCBI, highlighting the role of R1 ARGS in accelerating health and environmental risks.
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Genes Bacterianos , Aguas Residuales , Humanos , Antibacterianos/farmacología , Farmacorresistencia Microbiana/genética , Hospitales , Escherichia coliRESUMEN
Chemical dosing is the most used strategy for sulfide and methane abatement in urban sewer systems. Although conventional physicochemical methods, such as sulfide oxidation (e.g., oxygen/nitrate), precipitation (e.g., iron salts), and pH elevation (e.g., magnesium hydroxide/sodium hydroxide) have been used since the last century, the high chemical cost, large environmental footprint, and side-effects on downstream treatment processes demand a sustainable and cost-effective alternative to these approaches. In this paper, we aimed to review the currently used chemicals and significant progress made in sustainable sulfide and methane abatement technology, including 1) the use of bio-inhibitors, 2) in situ chemical production, and 3) an effective dosing strategy. To enhance the cost-effectiveness of chemical applications in urban sewer systems, two research directions have emerged: 1) online control and optimization of chemical dosing strategies and 2) integrated use of chemicals in urban sewer and wastewater treatment systems. The integration of these approaches offers considerable system-wide benefits; however, further development and comprehensive studies are required.
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Aguas del Alcantarillado , Purificación del Agua , Sulfuros , Nitratos , MetanoRESUMEN
Decentralization is recognized as an emerging solution for a more sustainable urban wastewater system (UWS) for the future. However, the debate of centralization vs. decentralization at the system's planning stage remains unresolved, mainly due to the complexity of the system's spatial structure and the multiple design objectives, such as water reuse and energy conservation. This paper presents the Sustainable Urban Wastewater System Generator (SUWStor) as a tool to address this issue. Integrating a graph representation of the system structure and the ant colony algorithm, SUWStor can produce Pareto optimal solutions for system design under three objectives: minimizing the capital cost, minimizing the operational energy consumption, and maximizing the water reuse capacity. The model is used for system design in a 100-square-km new city, the Xiong'an New District in China. Compared to the solution based on human experience, the model can reduce the system's capital cost by 7% and the operational energy in the pipe network by 26%, while maintaining the water reuse capacity at 100%. With this model, the relation between the optimal system layout and the choice over different design objectives can be discussed for any given area. In our case study, the optimal capacity of WWTPs for the lowest-cost solution is 48,000 m3 per day, leading to a total number of WWTPs of 5. As the water reuse level increases to maximum, the optimal capacity reduces to 15,000 m3 per day, where the number of WWTPs is 16. The model is also able to perform significantly better than the locally optimized results, in which only the WWTP locations are fixed at their optimal values. This demonstrates the importance of a global optimization model in designing the integrated UWS.
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Aguas Residuales , Purificación del Agua , Humanos , Ciudades , China , Agua , Algoritmos , Eliminación de Residuos Líquidos/métodosRESUMEN
1,4-Dioxane is a recalcitrant pollutant in water and is ineffectively removed during conventional water and wastewater treatment processes. In this study, we demonstrate the application of nitrifying sand filters to remove 1,4-dioxane from domestic wastewater without the need for bioaugmentation or biostimulation. The sand columns were able to remove 61 ± 10% of 1,4-dioxane on average (initial concentration: 50 µg/L) from wastewater, outperforming conventional wastewater treatment approaches. Microbial analysis revealed the presence of 1,4-dioxane degrading functional genes (dxmB, phe, mmox, and prmA) to support biodegradation being the dominant degradation pathway. Adding antibiotics (sulfamethoxazole and ciprofloxacin), that temporarily inhibited the nitrification process during the dosing period, showed a minor effect in 1,4-dioxane removal (6-8% decline, p < 0.05), suggesting solid resilience of the 1,4-dioxane-degrading microbial community in the columns. Columns amended with sodium azide significantly (p < 0.05) depressed 1,4-dioxane removal in the early stage of dosing but followed by a gradual increase of the removal over time to >80%, presumably due to a shift in the microbial community toward azide-resistant 1,4-dioxane degrading microbes (e.g., fungi). This study demonstrated for the first time the resilience of the 1,4-dioxane-degrading microorganisms during antibiotic shocks, and the selective enrichment of efficient 1,4-dioxane-degrading microbes after azide poisoning. Our observation could provide insights into designing better 1,4-dioxane remediation strategies in the future.
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Aguas Residuales , Contaminantes Químicos del Agua , Agua , Azidas , Dioxanos/metabolismo , Antibacterianos , Contaminantes Químicos del Agua/metabolismoRESUMEN
Triclocarban, one of the emerging pollutants, has been accumulating, and it is frequently detected in wastewater. Due to its toxicity and persistence, the efficient removal of triclocarban from wastewater systems is challenging. Genetic bioaugmentation with transferable catabolic plasmids has been considered to be a long-lasting method to clean up pollutants in continuous flow wastewater treatment systems. In this study, bioaugmentation with Pseudomonas putida KT2440, harboring the transferrable triclocarban-catabolic plasmid pDCA-1-gfp-tccA2, rapidly converted 50 µM triclocarban in wastewater into 3,4-dichloroaniline and 4-chloroaniline, which are further mineralized more easily. RT-qPCR results showed that the ratio of the copy number of pDCA-1-gfp-tccA2 to the cell number of strain KT2440 gradually increased during genetic bioaugmentation, suggesting horizontal transfer and proliferation of the plasmid. By using DNA stable isotope probing (SIP) and amplicon sequencing, OTU86 (Escherichia-Shigella), OTU155 (Citrobacter), OTU5 (Brucella), and OTU15 (Enterobacteriaceae) were found to be the potential recipients of the plasmid pDCA-1-gfp-tccA2 in the wastewater bacterial community. Furthermore, three transconjugants in the genera of Escherichia, Citrobacter, and Brucella showing triclocarban-degrading abilities were isolated from the wastewater. This study develops a new method for removing triclocarban from wastewater and provides insights into the environmental behavior of transferrable catabolic plasmids in bacterial community in wastewater systems.
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Contaminantes Ambientales , Pseudomonas putida , Carbanilidas , Contaminantes Ambientales/metabolismo , Plásmidos/genética , Pseudomonas putida/genética , Pseudomonas putida/metabolismo , Aguas ResidualesRESUMEN
The fate and formation of perfluoroalkyl acids (PFAAs) have been investigated during wastewater treatment processes but studies for the entire urban wastewater system comprising the sewage transport and wastewater and sludge treatment processes are scarce. This work performs an integrated assessment of the formation and fate of PFAAs in the urban wastewater system together with their behavior in separate components of the system. To achieve this, PFAAs were monitored over five weeks in a laboratory-scale urban wastewater system comprising sewer reactors, a wastewater treatment reactor, and an anaerobic sludge digester. The system was fed with real domestic wastewater. The total mass of 11 PFAAs flowing out of the laboratory wastewater system significantly (p < 0.05) increased by 112 ± 14 (mean ± standard error)% compared to that entering the system. Formation of PFAAs was observed in all three biological processes of the system. In anaerobic sewer process, perfluoropentanoic acid (PFPeA), perfluoroheptanoic acid (PFHpA), and perfluorooctane sulfonate (PFOS) exhibited significant formation (p < 0.05) with the mass flow increased by 79 ± 24%, 109 ± 31%, and 57 ± 17%, respectively. During the wastewater treatment process, perfluorohexanoic acid (PFHxA), perfluorooctanoic acid (PFOA), and perfluorododecanoic acid (PFDoDA) demonstrated significant increase (p < 0.05) in their mass flows by 176 ± 56%, 92 ± 21%, and 516 ± 184%, respectively. In contrast, only PFHxA was found to significantly (p < 0.05) increase by 130 ± 40% during anaerobic digestion process. The total mass of 11 PFAAs discharged through the effluent (201 ± 24 ng day-1) was 5 times higher than that through the digested sludge (29 ± 6 ng day-1).
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Ácidos Alcanesulfónicos , Fluorocarburos , Contaminantes Químicos del Agua , Ácidos Alcanesulfónicos/análisis , Monitoreo del Ambiente , Fluorocarburos/análisis , Aguas del Alcantarillado , Aguas Residuales , Contaminantes Químicos del Agua/análisisRESUMEN
General resilience addresses the resilience of a water system to any threat including unknowns, in contrast to specified resilience to individual identified threats. However, quantification of general resilience is challenging and previous assessments have typically been qualitative or based on system properties that are assumed to be indicative of resilient performance. Here we present a General Resilience Assessment Methodology (GRAM), which uses a middle-state based approach to decompose general resilience into contributing components to provide a quantitative and performance-based resilience assessment. GRAM enables the accounting of the effects of any threat if all modes of system failure are identifiable. It is applied to an integrated urban wastewater system where five interventions are explored. The results obtained show that whilst substantial improvements in specified resilience are achieved, increasing the general resilience of the system is challenging. However, general resilience analysis enables identification of system failure modes to which level of service is least resilient and highlights key opportunities for intervention development. GRAM is beneficial as it can inform the development of interventions to increase the resilience of a system to unknowns such as unforeseeable natural hazards in a quantifiable manner.
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Aguas Residuales , AguaRESUMEN
This study measured the environmental impacts from three same-size wastewater treatment systems, specifically activated sludge, a constructed wetland, and a high rate algal pond. Detailed data inventories were employed using SimaPro 9 software to calculate the entire consequences by ReCiPe 2016 and Greenhouse Gas Protocol method. The environmental outcomes caused by substance emissions and resource extraction are presented in several impact categories at the endpoint level. For a better comparison, the single score tool was applied to aggregate all factors into three areas of protection: human health, ecosystem, and resource shortage. Results showed that concrete and steel are the main contributors to the construction phase, while electricity is responsible for the operation stage. The single score calculation indicates that the proportion of construction activities could be equal to or even higher than the operation stage for a small capacity plant. The total environmental impact of the conventional system was 2.3-fold and 3-fold higher than that of constructed wetland and high rate algal pond, respectively. High rate algal pond has the best environmental performance when generating the least burdens and greenhouse gas emissions of 0.72 kg CO2 equivalent per m3. Constructed wetland produces 5.69 kg CO2, higher than an algal pond but much lower than activated sludge plant, emitting 11.42 kg CO2 per m3.
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Gases de Efecto Invernadero , Purificación del Agua , Ecosistema , Efecto Invernadero , Humanos , Aguas del Alcantarillado , Eliminación de Residuos Líquidos , Aguas ResidualesRESUMEN
Information on sales and emission of selected pharmaceuticals were used to predict their concentrations in Japanese wastewater influent through a >300 of pharmaceuticals data sink. A combined wastewater-based epidemiology and environmental risk analysis follow was established. By comparing predicted environmental concentrations (PECs) of pharmaceuticals in wastewater influent against measured environmental concentrations (MECs) reported in previous studies, it was found that the model gave accurate results for 17 pharmaceuticals (0.5 < PEC/MEC < 2), and acceptable results for 32 out of 40 pharmaceuticals (0.1 < PEC/MEC < 10). Although the majority of pharmaceuticals considered in the model were antibiotics and analgesics, pranlukast, a receptor antagonist, was predicted to have the highest concentration in wastewater influent. With regard to the composition of wastewater effluent, the Estimation Program Interface (EPI) suite was used to predict pharmaceutical removal through activated sludge treatment. Although the performance of the EPI suite was variable in terms of accurate prediction of the removal of different pharmaceuticals, it could be an efficient tool in practice for predicting removal under extreme scenarios. By using the EPI suite with input data on PEC in the wastewater influent, the PEC values of pharmaceuticals in wastewater effluent were predicted. The concentrations of 26 pharmaceuticals were relatively high (>1 µg/L), and the PECs of 6 pharmaceuticals were extremely high (>10 µg/L) in wastewater effluent, which could be attributed to their high usage rates by consumers and poor removal rates in wastewater treatment plants (WWTPs). Furthermore, environmental risk assessment (ERA) was carried out by calculating the ratio of predicted no effect concentration (PNEC) to PEC of different pharmaceuticals, and it was found that 9 pharmaceuticals were likely to have high toxicity, and 54 pharmaceuticals were likely to have potential toxicity. It is recommended that this is further investigated in detail. The priority screening and environmental risk assessment results on pharmaceuticals can provide reliable basis for policy-making and environmental management.
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Preparaciones Farmacéuticas , Contaminantes Químicos del Agua , Monitoreo del Ambiente , Japón , Eliminación de Residuos Líquidos , Aguas Residuales/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
The use of coagulants and flocculants in the water and wastewater industry is predicted to increase further in the coming years. Alum is the most widely used coagulant, however, the use of ferric chloride (FeCl3) is gaining popularity. Drinking water production that uses FeCl3 as coagulant produces waste sludge rich in iron. We hypothesised that the iron-rich drinking water sludge (DWS) can potentially be used in the urban wastewater system to reduce dissolved sulfide in sewer systems, aid phosphate removal in wastewater treatment and reduce hydrogen sulfide in the anaerobic digester biogas. This hypothesis was investigated using two laboratory-scale urban wastewater systems, one as an experimental system and the other as a control, each comprising sewer reactors, a sequencing batch reactor (SBR) for wastewater treatment, sludge thickeners and anaerobic digestion reactors. Both were fed with domestic wastewater. The experimental system received in-sewer DWS-dosing at 10 mgFe L-1 while the control had none. The sulfide concentration in the experimental sewer effluent decreased by 3.5 ± 0.2 mgS L-1 as compared with the control, while the phosphate concentration decreased by 3.6 ± 0.3 mgP L-1 after biological wastewater treatment in the experimental SBR. The dissolved sulfide concentration in the experimental anaerobic digester also decreased by 15.9 ± 0.9 mgS L-1 following the DWS-dosing to the sewer reactors. The DWS-doing also enhanced the settleability of the mixed liquor suspended sludge (MLSS) (SVI decreased from 193.2 ± 22.2 to 108.0 ± 7.7 ml g-1), and the dewaterability of the anaerobically digested sludge (the cake solids concentration increased from 15.7 ± 0.3% to 19.1 ± 1.8%). The introduction of DWS into the experimental system significantly increased the COD and TSS concentrations in the wastewater, and consequently the MLSS concentration in the SBR, however, this did not affect normal operation. The results demonstrated that iron-rich waste sludge from drinking water production can be used in the urban wastewater system achieving multiple benefits. Therefore, an integrated approach to urban water and wastewater management should be considered to maximise the benefits of iron use in the system.
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Agua Potable , Aguas Residuales , Reactores Biológicos , Hierro , Aguas del Alcantarillado , Eliminación de Residuos LíquidosRESUMEN
Sustainability and resilience are both key considerations in the design and operation of wastewater systems. However, there is currently a lack of understanding of the relationship between these two goals and of the effects of increasing resilience on sustainability. This paper, therefore, presents a framework for analysis of the effects of resilience-enhancing interventions on sustainability, and applies this to an urban wastewater system. Given that sustainability addresses the long term, the framework includes a novel sustainability assessment approach which captures a continuum of potential future conditions and enables identification of tipping points where applicable. This method allows a wide range of potential futures to be captured whilst removing the need to develop scenarios or future projections. While it may be possible to develop interventions that are beneficial in terms of their effects on both resilience and sustainability, the results obtained from the case study demonstrate that implementing measures designed to increase resilience of an integrated urban wastewater system does not guarantee a universal improvement in sustainability. Therefore, when proposing measures to increase resilience, the potential effects on sustainability should be considered also. It is also shown that the extent of any negative effects on system sustainability can vary significantly depending on future conditions, with the case study intervention (increasing pump capacity) achieving the highest degree of sustainability if rainfall depths or imperviousness in the catchments reduce. However, trade-offs between sustainability indicators are present irrespective of future conditions. Furthermore, while an intervention that enhances resilience may be considered sustainable with respect to specific indicators under current conditions, tipping points exist and it will cease to be sustainable if future threat magnitudes exceed these.
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Modelos Teóricos , Aguas Residuales , PredicciónRESUMEN
Reliability, risk and resilience are strongly related concepts and have been widely utilised in the context of water infrastructure performance analysis. However, there are many ways in which each measure can be formulated (depending on the reliability of what, risk to what from what, and resilience of what to what) and the relationships will differ depending on the formulations used. This research has developed a framework to explore the ways in which reliability, risk and resilience may be formulated, identifying possible components and knowledge required for calculation of each and formalising the conceptual relationships between specified and general resilience. This utilises the Safe & SuRe framework, which shows how threats to a water system can result in consequences for society, the economy and the environment, to enable the formulations to be derived in a logical manner and to ensure consistency in any comparisons. The framework is used to investigate the relationship between levels of reliability, risk and resilience provided by multiple operational control and design strategies for an urban wastewater system case study. The results highlight that, although reliability, risk and resilience values may exhibit correlations, designing for just one is insufficient: reliability, risk and resilience are complementary rather than interchangeable measures and one cannot be used as a substitute for another. Furthermore, it is shown that commonly used formulations address only a small fraction of the possibilities and a more comprehensive assessment of a system's response to threats is necessary to provide a comprehensive understanding of risk and resilience.
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Aguas Residuales , Agua , Reproducibilidad de los ResultadosRESUMEN
End-of-pipe permitting is a widely practised approach to control effluent discharges from wastewater treatment plants. However, the effectiveness of the traditional regulation paradigm is being challenged by increasingly complex environmental issues, ever growing public expectations on water quality and pressures to reduce operational costs and greenhouse gas emissions. To minimise overall environmental impacts from urban wastewater treatment, an operational strategy-based permitting approach is proposed and a four-step decision framework is established: 1) define performance indicators to represent stakeholders' interests, 2) optimise operational strategies of urban wastewater systems in accordance to the indicators, 3) screen high performance solutions, and 4) derive permits of operational strategies of the wastewater treatment plant. Results from a case study show that operational cost, variability of wastewater treatment efficiency and environmental risk can be simultaneously reduced by at least 7%, 70% and 78% respectively using an optimal integrated operational strategy compared to the baseline scenario. However, trade-offs exist between the objectives thus highlighting the need of expansion of the prevailing wastewater management paradigm beyond the narrow focus on effluent water quality of wastewater treatment plants. Rather, systems thinking should be embraced by integrated control of all forms of urban wastewater discharges and coordinated regulation of environmental risk and treatment cost effectiveness. It is also demonstrated through the case study that permitting operational strategies could yield more environmentally protective solutions without entailing more cost than the conventional end-of-pipe permitting approach. The proposed four-step permitting framework builds on the latest computational techniques (e.g. integrated modelling, multi-objective optimisation, visual analytics) to efficiently optimise and interactively identify high performance solutions. It could facilitate transparent decision making on water quality management as stakeholders are involved in the entire process and their interests are explicitly evaluated using quantitative metrics and trade-offs considered in the decision making process. We conclude that the operational strategy-based permitting shows promising for regulators and water service providers alike.
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Eliminación de Residuos Líquidos , Calidad del Agua , Ambiente , Aguas ResidualesRESUMEN
The first flush effect in combined sewer systems during storm events often causes overflows and overloads of the sewage treatment, which reduces the efficiency of the sewage treatment and decreases the quality of the receiving waters due to the pollutants that are contributed. The use of retention tanks constitutes a widely used way to mitigate this effect. However, the management of the pollutant loads encounters difficulties when the retention tanks are emptied. A new approach is proposed to solve this problem by fulfilling the treatment requirements in real time, focussing on the characteristics of the wastewater. The method is based on the execution of an Ant Colony Optimisation algorithm to obtain a satisfactory sequence for the discharge of the retention tanks. The discharge sequence considers the volume of stormwater and its concentration of pollutants including Suspended Solids, Biological Oxygen Demand and Chemical Oxygen Demand, Total Nitrogen and Total Phosphorus. The Ant Colony Optimisation algorithm was applied successfully to a case study with overall reduction of pollutant loads stored in retention tanks. The algorithm can be adapted in a simple way to the different scenarios, infrastructures and controllers of sewer systems.