RESUMEN

The presence of antibiotics can affect the natural microbial community and exert selective pressure on the environment's microorganisms. This study focused on three types of urban landscape lakes in Xi'an that were closely related to human activities. By combining basic water quality indicators, antibiotic occurrence status, bacterial communities and their potential metabolic functions, Spearman correlation coefficient and redundancy analysis were used to explore the relationship between them, and further explore the impact mechanism of environmental factors and antibiotics on bacterial community structure. The results showed that ofloxacin, erythromycin, and roxithromycin were the main types of antibiotics in the three landscape lakes, with low ecological risks, and there was a clear clustering of antibiotic occurrence. Proteobacteria was the most abundant bacterial phylum, and each lake had its own unique dominant bacteria, which indicates that they are influenced by varying water sources, pollution, and other nearby environments. Statistical analysis showed that pH and nitrogen nutrients were the most critical environmental factors affecting bacterial communities (P<0.01), while tetracyclines and lincomycins were the antibiotics that had a significant impact on bacterial communities (P<0.05). Antibiotics mainly promote defense- and signal transduction-related functions, and inhibit the metabolic activity of bacterial communities. However, the impact of antibiotics on bacterial diversity, community structure, and potential metabolic function in the three urban lakes was less than that of environmental factors. These results help to clarify the mechanism and degree of impact of different interference factors (environmental factors, conventional pollutants, and antibiotics) on bacterial communities in the water environment and are important for the management of urban landscape lake water environments.

Asunto(s)

Lagos , Contaminantes Químicos del Agua , Humanos , Lagos/química , Antibacterianos/farmacología , Contaminantes Químicos del Agua/toxicidad , Bacterias , Proteobacteria , China

RESUMEN

Urban landscape lakes are closely related to human activity, but there are limited studies on their bacterial community characteristics and risks to human health. In this study, four different types of urban landscape lakes in Xi'an were selected, and the bacterial community structures in different seasons were analyzed by Illumina Nova high-throughput sequencing technology. Seasonal variations in bacterial communities were analyzed by linear discriminant analysis, STAMP difference analysis, and nonmetric multidimensional scaling. Redundancy analysis was used to investigate the influencing factors. Furthermore, the metabolic functions of bacterial communities were predicted by Tax4Fun. There were clear seasonal differences in the α-diversity of bacteria, with bacterial diversity being higher in winter than in summer in the four urban landscape lakes, and the diversity of different water sources was different; the distributions of Proteobacteria, Actinobacteria, Chloroflexi, and Verrucomicrobia had significant seasonal differences; and the dominant bacteria at the genus level had obvious temporal and spatial differences. Furthermore, a variety of environmental factors had an impact on bacterial communities, and temperature, DO, and nitrogen were the primary factors affecting the seasonal variation in bacteria. There are also significant seasonal differences in the metabolic functions of bacterial communities. These results are helpful for understanding the current status of bacteria in the aquatic environments of such urban landscape lakes.

Asunto(s)

Actinobacteria , Lagos , Humanos , Lagos/microbiología , Bacterias/genética , Proteobacteria , Estaciones del Año , China , ARN Ribosómico 16S/genética

RESUMEN

Urban landscape lakes (ULLs) are important environmental elements in most cities. In order to understand the current situation of ULLs in China and formulate proper strategies to improve their landscape quality to meet public desire for water-front enjoyment, a study was conducted of 189 ULLs widely distributed in 26 provinces of China, based on existing data and field surveys. These ULLs were firstly categorized according to their topographic features, climatic zones, and water replenishment sources. Lake water quality was evaluated considering both single factors and a comprehensive pollution index (CPI). Results show that if the Chinese Surface Water Quality Standard was used as the sole criteria, about 60% of the ULLs investigated could not meet the lowest requirement. Excessive total nitrogen (TN) concentration was the most limiting factor especially when reclaimed water was the replenishment source. The differences in topographic and climatic conditions to a certain extent affected the availability of replenishment water sources but no significant correlation was identified with the single water quality factors or CPI. However, when public satisfaction was introduced in the evaluation of the ULLs' landscape effect, it was found that the water transparency in terms of Secchi Depth (SD) correlated well with people's appreciation of water landscape.