RESUMEN
One hundred and twenty-nine isolates of Trichosporon jirovecii were isolated from the melanized exoskeleton as well as eyestalks, gills, muscle and haemolymph of red swamp crayfish (Procambarus clarkii) collected from the River Nile, during summer 2015. Isolates were similar morphologically, biochemically and genetically. Also, random amplified polymorphic DNA (RAPD) analysis exhibited no polymorphism among the tested isolates. Virulence factors such as chitinase, protease, lipase activities and biofilm formation were examined. Challenge test, using a representative isolate (Tj_ASU8), proved its pathogenicity against crayfish. Magnesium oxide nanoparticles had a good antifungal activity with a minimum fungicidal concentration of 8 mg/ml. To the best of our knowledge, this is the first report for isolation of T. jirovecii from red swamp crayfish, showing melanization, from the River Nile. We assume that infected crayfish may act as a vector for this fungus and can disseminate infection to all susceptible hosts in the vicinity.
Asunto(s)
Astacoidea/microbiología , Trichosporon/clasificación , Trichosporon/fisiología , Animales , ADN de Hongos/análisis , Filogenia , Análisis de Secuencia de ADN , Trichosporon/genéticaRESUMEN
This report describes the isolation of two environmental fungi: Rhodotorula mucilaginosa and Trichosporon jirovecii accompanied by Pseudomonas aeruginosa and Escherichia coli from a dog with bronchotracheitis. All microorganisms were isolated routinely from a mucopurulent discharge, obtained during bronchoscopy from laryngotracheal area. The initial identification of yeasts was confirmed by API Candida and by molecular analysis of internal transcribed spacer region. Administered antimicrobial treatment with Marbofloxacin and Canizol has brought the improvement in the dogs' health status. The final results of control mycological culture were negative. Most probably underlying hypothyroidism and the use of steroids were the factors predisposing this patient to opportunistic infection of mixed aetiology. As far as we are concerned, this is the first case of dogs' respiratory tract infection caused by R. mucilaginosa and T. jirovecii.
Asunto(s)
Coinfección/veterinaria , Enfermedades de los Perros/diagnóstico , Escherichia coli/aislamiento & purificación , Pseudomonas aeruginosa/aislamiento & purificación , Infecciones del Sistema Respiratorio/veterinaria , Rhodotorula/aislamiento & purificación , Trichosporon/aislamiento & purificación , Animales , Antibacterianos/administración & dosificación , Antifúngicos/administración & dosificación , Broncoscopía , Análisis por Conglomerados , Coinfección/diagnóstico , Coinfección/microbiología , Coinfección/patología , ADN Bacteriano/química , ADN Bacteriano/genética , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Enfermedades de los Perros/microbiología , Enfermedades de los Perros/patología , Perros , Escherichia coli/clasificación , Escherichia coli/genética , Femenino , Técnicas Microbiológicas , Filogenia , Pseudomonas aeruginosa/clasificación , Pseudomonas aeruginosa/genética , Infecciones del Sistema Respiratorio/diagnóstico , Infecciones del Sistema Respiratorio/microbiología , Infecciones del Sistema Respiratorio/patología , Rhodotorula/clasificación , Rhodotorula/genética , Análisis de Secuencia de ADN , Resultado del Tratamiento , Trichosporon/clasificación , Trichosporon/genéticaRESUMEN
Cutaneous mycoses have been rarely reported in Chelonians. A Testudo hermanni adult male showed a thick erosion of the dorsal neck covered by necrotic material. Trichosporon sp. was cultivated, while arthrospores and hyphae were observed in histological sections. The causative agent was identified as Trichosporon jirovecii by PCR. After a surgical intervention povidone iodine and Iruxol® ointment were daily administered through the drainage for 2 weeks, along with enrofloxacin 5 mg diluted in saline 0.5 ml via the intracelomatic route. After treatment the ulcer healed with residual scars. No relapse was registered after 12 months. T. jirovecii is considered as a rare yeast pathogen and the presented case is the first report of a dermatomycosis in tortoises caused by this yeast species.
RESUMEN
Cadmium sulphide is one of the most promising materials for solar cells and of great interest due to its useful applications in photonics and electronics, thus the development of bio-mediated synthesis of cadmium sulphide nanoparticles (CdS NPs) is one of the essential areas in nanoparticles. The present study demonstrates for the first time the eco-friendly biosynthesis of CdS NPs using the yeast Trichosporon jirovecii. The biosynthesis of CdS NPs were confirmed by UV-Vis spectrum and characterized by X-ray diffraction assay and electron microscopy. Scanning and transmission electron microscope analyses shows the formation of spherical CdS NPs with a size range of about 6-15 nm with a mean Cd:S molar ratio of 1.0:0.98. T. jirovecii produced hydrogen sulfide on cysteine containing medium confirmed by positive cysteine-desulfhydrase activity and the colony color turned yellow on 0.1 mM cadmium containing medium. T. jirovecii tolerance to cadmium was increased by the UV treatment and three 0.6 mM cadmium tolerant mutants were generated upon the UV radiation treatment. The overall results indicated that T. jirovecii could tolerate cadmium toxicity by its conversion into CdS NPs on cysteine containing medium using cysteine-desulfhydrase as a defense response mechanism.