RESUMEN

In the study, a new triple-helix polysaccharide with favorable stability was purified from C. cornucopioides. Its structural characterization, stability and solution behavior were investigated by the GC-MS, periodate oxidation-smith degradation, FT-IR, 1D and 2D NMR spectroscopy, methylation analysis, Scanning electron microscope, Congo-red, CD, TGA and DSC analysis. The results showed that Craterellus cornucopioide polysaccharide (CCP) possessed the molecular weight of 1.97 × 103 kDa, is mainly composed of mannose (48.73%), galactose (17.37%), glucose (15.97%) and xylose (17.93%), respectively. It was a heteroglycan with (1 → 3)­linked­ß­d­Manp­(1 → 6)­linked α­d­Galp backbone distributed by (1 → 4)­linked­α­d­Xylp­t­α­d­Manp and t­ß­d­Glup units at O-6. The result of TGA and DSC assay indicated that CCP has a favorable thermal stability. MTT and Scanning electro microscopy (SEM) assay showed that CCP could significantly improve the proliferation activity and induce cells activation of RAW264.7 in a certain range of concentrations and period.

Asunto(s)

Fenómenos Químicos , Factores Inmunológicos/química , Factores Inmunológicos/farmacología , Macrófagos/citología , Polisacáridos/química , Polisacáridos/farmacología , Agaricales , Animales , Rastreo Diferencial de Calorimetría , Espectroscopía de Resonancia Magnética con Carbono-13 , Proliferación Celular/efectos de los fármacos , Forma de la Célula/efectos de los fármacos , Dicroismo Circular , Rojo Congo , Macrófagos/efectos de los fármacos , Metilación , Ratones , Microscopía de Fuerza Atómica , Conformación Molecular , Peso Molecular , Polisacáridos/aislamiento & purificación , Espectroscopía de Protones por Resonancia Magnética , Células RAW 264.7 , Soluciones , Espectroscopía Infrarroja por Transformada de Fourier , Termogravimetría