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Soybean hulls are lignocellulosic residuesgeneratedinthe industrial processing of soybean, representing about 5 % of the mass of the whole bean. This by-product isan importantsource of polymers suchas cellulose(34 %) and hemicellulose (11 %),which could bevalorizedvia biotechnology to improvethe economic returnof the oilseed chain. In the present work,soybean hulls were evaluated as a carbon sourcefor biolipid productionbyLipomycesstarkeyi LPB 53. Initially the hulls were treated physicochemically and enzymatically to obtain fermentable sugars. Subsequently, biomass growth was evaluated using different nitrogen sources andthe lipid production was optimized, reaching a maximum cell biomass concentration of 26.5 g/L with 42.5 % of lipids. Around 65 % of the xylose content was consumed.The obtained oil wasmajorlycomposed of oleic, palmitic, palmitoleic, linoleic and stearic fatty acids in a proportion of 54 %, 32 %, 4 %, 3 % and 2 %, respectively.

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ABSTRACT: This study evaluated the action of commercial and non-commercial cellulases and pectinases in the hydrolysis of soybean hulls (SH) and corn stover and cobs (CSC), the effect of temperature and agitation on the lignocellulosic substrate hydrolysis and the bromatological characteristics of hydrolyzed substrates. The effect of pretreatment on the hydrolysis of lignocellulosic residues and bromatological analysis were also evaluated. The highest hydrolytic activity occurred at 300 rpm for SH (47.95 and 51.43% for cellulase and pectinase, respectively) and at 350 rpm for CSC (26.05 and 9.23% for cellulase and pectinase, respectively). Non-commercial enzymes achieved 7.26-30% of the amount of hydrolysis obtained with commercial enzymes, on the same substrates. Pretreatment with 7.5% of NaOH and a particle size of the substrate of 0.5 mm significantly increased the hydrolysis of SH and CSC for both enzymes. The bromatological characteristics showed that soybean hulls hydrolyzed with both commercial cellulase and pectinase have potential for large-scale use in animal feed production.

RESUMO: Foram avaliadas a ação de celulases e pectinases comerciais e não comerciais na hidrólise de casca de soja (CS) e palha e espigas de milho (PEM), o efeito da temperatura e da agitação na hidrólise do substrato lignocelulósico e as características bromatológicas dos substratos hidrolisados. O efeito do pré-tratamento na hidrólise de resíduos lignocelulósicos e a análise bromatológica também foram avaliados. A maior atividade hidrolítica ocorreu a 300 rpm para CS (47,95 e 51,43% para celulase e pectinase, respectivamente) e a 350 rpm para PEM (26,05 e 9,23% para celulase e pectinase, respectivamente). As enzimas não comerciais atingiram 7,26-30% da quantidade de hidrólise obtida com as enzimas comerciais, nos mesmos substratos. O pré-tratamento com 7,5% de NaOH e um tamanho de partícula do substrato de 0,5 mm aumentou significativamente a hidrólise de CS e PEM para ambas as enzimas. As características bromatológicas mostraram que a casca de soja hidrolisada com celulase e pectinase comercial tem potencial para uso em larga escala na produção de ração animal.

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Triclosan (TCS) is widely used in the production of antibacterial products, being often found in wastewater. Therefore, this study developed new materials via soybean hulls (SBHF) and açaí seeds (AÇSF) functionalization with iron oxide nanoparticles to be applied in the TCS adsorption. The characterization confirmed the functionalization of the materials. The adsorption results indicated that the equilibrium of the process occurred after 480 and 960 min for SBHF and AÇSF, respectively. The maximum adsorptive capacity values were 158.35 and 155.09 mg g-1 for SBHF and AÇSF, respectively, at 318 K. The kinetic and isothermal data better fitted to the pseudo-second-order and Langmuir models. Thermodynamics indicated that the processes had an endothermic, spontaneous, and reversible character. The main adsorption mechanisms were H-bond and π-interactions. The pH and ionic strength studies indicated that the adsorption efficiency has not been reduced pronouncedly. The biosorbents reuse was effective for five cycles. In the synthetic mixture, the removal rate was satisfactory (92.53% and 57.02%, respectively for SBHF and AÇSF). These results demonstrate the biosorbents high potential for large-scale application.

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Breast cancer is the leading cause of cancer death among women worldwide. For this reason, the development of new therapies is still essential. In this work we have analyzed the antitumor potential of levoglucosenone, a chiral building block derived from the pyrolysis of cellulose-containing materials such as soybean hulls, and three structurally related analogues. Employing human and murine mammary cancer models, we have evaluated the effect of our compounds on cell viability through MTS assay, apoptosis induction by acridine orange/ethidium bromide staining and/or flow cytometry and the loss of mitochondrial potential by tetramethylrhodamine methyl ester staining. Autophagy and senescence induction were also evaluated by Western blot and ß-galactosidase activity respectively. Secreted metalloproteases activity was determined by quantitative zymography. Migratory capacity was assessed by wound healing assays while invasive potential was analyzed using Matrigel-coated transwell chambers. In vivo studies were also performed to evaluate subcutaneous tumor growth and experimental lung colonization. All compounds impaired in vitro proliferation with IC50 values in a range of low micromolar. Apoptosis was identified as the main mechanism responsible for the reduction of monolayer cell content induced by the compounds without detecting modulations of autophagy or senescence processes. Two of the four compounds (levoglucosenone and its brominated variant) were able to modulate in vitro events associated with tumor progression, such as migratory potential, invasiveness, and proteases secretion. Furthermore, tumor volume and metastatic spread were significantly reduced in vivo after the treatment these two compounds. Here, we could obtain from soybean hulls, a material with almost no commercial value, a variety of chemical compounds useful for breast cancer treatment.

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Soybean is one of the major world crops, with an annual production of 359 million tons. Each ton of processed soybean generates 50-80 kg of soybean hulls (SHs), representing 5-8% of the whole seed. Due to environmental concerns and great economic potential, the search of SHs re-use solutions are deeply discussed. The lignocellulosic composition of SHs has attracted the attention of the scientific and productive sector. Recently, some studies have reported the use of SHs in the production of medium to high value-added molecules, with potential applications in food and feed, agriculture, bioenergy, and other segments. This review presents biotechnological approaches and processes for the management and exploitation of SHs, including pre-treatment methods and fermentation techniques, for the production of different biomolecules. Great potentialities and innovations were found concerning SH exploration and valorisation of the soybean chain under a biorefinery and circular bioeconomy optic.

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The aim of this study was to develop optimized enzyme cocktails, containing native and recombinant purified enzymes from five fungal species, for the saccharification of alkali- and acid-pretreated sugarcane bagasse (SCB), soybean hulls (SBH) and oil palm empty fruit bunches (EFB). Basic cellulases were represented by cellobiohydrolase I (CBH) and endo-glucanase II (EG) from Penicillium verruculosum and ß-glucosidase (BG) from Aspergillus niger. Auxiliary enzymes were represented by endo-xylanase A (Xyl), pectin lyase (PNL) and arabinoxylanhydrolase (AXH) from Penicillium canescens, ß-xylosidase (BX) from Aspergillus japonicus, endo-arabinase (ABN) from A. niger and arabinofuranosidase (Abf) from Aspergillus foetidus. Enzyme loads were 5 mg protein/g dry substrate (basic cellulases) and 1 mg/g (each auxiliary enzyme). The best choice for SCB and EFB saccharification was alkaline pretreatment and addition of Xyl + BX, AXH + BX or ABN + BX + Abf to basic cellulases. For SBH, acid pretreatment and basic cellulases combined with ABN + BX + Abf or Xyl + BX performed better than other enzyme preparations.

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Asthma epidemics have been shown to be related to where soybeans are loaded and handled, but data are scarce in the literature. This pilot study evaluated the levels of Gly m 1 in dust samples collected in Maringá, Brazil, a city with high soy production and processing. A dust impactor was used to collect seven isolated samples during 2015 and 2016. Samples were analyzed by an ELISA (enzyme-linked immunosorbent assay) detection method. Gly m 1 was found in all samples, ranging from 0.82-24.38 ng/m3 (median 2.41), regardless of the month or year evaluated. The levels of Gly m 1 were considered low, but the concentrations required to cause sensitization and symptoms are uncertain.

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Production of 2,3-butanediol (2,3-BD) by Pantoea agglomerans strain BL1 was investigated using soybean hull hydrolysate as substrate in batch reactors. The cultivation media consisted of a mixture of xylose, arabinose, and glucose, obtained from the hemicellulosic fraction of the soybean hull biomass. We evaluated the influence of oxygen supply, pH control, and media supplementation on the growth kinetics of the microorganism and on 2,3-BD production. P. agglomerans BL1 was able to simultaneously metabolize all three monosaccharides present in the broth, with average conversions of 75% after 48 h of cultivation. The influence of aeration conditions employed demonstrated the mixed acid pathway of 2,3-BD formation by enterobacteria. Under fully aerated conditions (2 vvm of air), up to 14.02 g L-1 of 2.3-BD in 12 h of cultivation were produced, corresponding to yields of 0.53 g g-1 and a productivity of 1.17 g L-1 h-1, the best results achieved. These results suggest the production potential of 2,3-BD by P. agglomerans BL1, which has been recently isolated from an environmental consortium. The present work proposes a solution for the usage of the hemicellulosic fraction of agroindustry biomasses, carbohydrates whose utilization are not commonly addressed in bioprocess.

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The aim of this work was to develop a new production, recovery and formulation process of gibberellic acid (GA3). Low-cost byproducts - citrus pulp (CP) and soybean hulls (SH) - were employed as substrate for GA3 production by Gibberella fujikuroi in semisolid fermentation. A CP/SH mixture (70%/30%) promoted high productivities both in bubble column reactor (1.66 mg L/h), and in stirred tank reactor (2.13 mg L/h). GA3 production medium cost (US$ 6.70/m3) was reduced by 85% when compared to previously reported synthetic media (US$ 44.96/m3). It was described that GA3 fermented extract has low stability, and that liquid and powder formulation of the fermented extract maintained the biomolecule activity over 6 months. Alginate and alginate/kefiran beads containing GA3 showed encapsulation efficiency of 70% and 60%, respectively. This work supports good perspectives for GA3 production using cheap substrates and simple formulation of clarified extract to favour its use in agricultural countries.

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The present study was conducted to investigate the effects of dietary fiber source levels on the fecal ammonia nitrogen, growth performance, carcass traits, gastrointestinal tract development, and intestinal morphology of broilers. A total of 420 one-day-old unsexed broiler chicks were individually weighed and randomly divided into 5 groups, each with seven replicates of twelve chicks. Rice hulls (RH) and soybean hulls (SH) were ground through a hammer mill with a 2-mm screen. The RH and SH experimental diets were as follows: 0% (control); 2.5% RH; 2.5% SH; 5% RH; and 5% SH. No significant differences were found in growth performance and fecal ammonia nitrogen among the dietary treatment groups (p>0.05). Compared with the control, the experimental diets with 2.5% SH significantly decreased the wing weight of chickens (p 0.05), while no significant differences in the weight of the other visceral organs were observed. Compared with the control, broilers in the 5% SH group had a longer jejunum and ileum (p 0.05). Feeding the broilers SH and RH had no effect on the villus area and crypt depth of the intestine. Compared with the control, the experimental diet with 2.5% RH significantly increased the duodenal villus height of chickens (p 0.05). These findings suggest that the inclusion of 5% SH in the diets resulted in improved intestinal morphology without negatively affecting growth performance and carcass traits.(AU)

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The present study was conducted to investigate the effects of dietary fiber source levels on the fecal ammonia nitrogen, growth performance, carcass traits, gastrointestinal tract development, and intestinal morphology of broilers. A total of 420 one-day-old unsexed broiler chicks were individually weighed and randomly divided into 5 groups, each with seven replicates of twelve chicks. Rice hulls (RH) and soybean hulls (SH) were ground through a hammer mill with a 2-mm screen. The RH and SH experimental diets were as follows: 0% (control); 2.5% RH; 2.5% SH; 5% RH; and 5% SH. No significant differences were found in growth performance and fecal ammonia nitrogen among the dietary treatment groups (p>0.05). Compared with the control, the experimental diets with 2.5% SH significantly decreased the wing weight of chickens (p 0.05), while no significant differences in the weight of the other visceral organs were observed. Compared with the control, broilers in the 5% SH group had a longer jejunum and ileum (p 0.05). Feeding the broilers SH and RH had no effect on the villus area and crypt depth of the intestine. Compared with the control, the experimental diet with 2.5% RH significantly increased the duodenal villus height of chickens (p 0.05). These findings suggest that the inclusion of 5% SH in the diets resulted in improved intestinal morphology without negatively affecting growth performance and carcass traits.

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Objetivou-se avaliar três períodos de adaptação, cinco, 10 e 15 dias, a dietas com baixa ou alta fibra sobre a digestibilidade e características fecais. Foram utilizadas duas dietas, uma contendo 0% de casca de soja (0%CS) e outra com 15% de casca de soja (15%CS), fornecidas a 12 cães adultos. Ao final de cada período, houve coleta total de fezes para avaliação dos coeficientes de digestibilidade aparente (CDA), energia metabolizável (EM) e características fecais. Além disso, foi avaliada a palatabilidade das dietas. Como resultado, não houve efeito do período de adaptação sobre a digestibilidade das dietas (P>0,05). A dieta 15%CS apresentou menor CDA da matéria seca (MS) e EM, em relação à dieta 0%CS (P<0,05). A consistência fecal dos cães não diferiu entre as dietas e os períodos (P>0,05). Houve redução no pH e na amônia fecal dos cães alimentados com a dieta 15%CS (P<0,05), após 10 dias de alimentação. Não houve diferença na palatabilidade das dietas (P>0,05). Períodos de adaptação à dieta superiores a cinco dias não alteram a digestibilidade e a maioria das características fecais de cães, exceto a amônia fecal. A inclusão de 15%CS na dieta reduz a digestibilidade da MS e a EM, bem como o pH e a amônia fecal.(AU)

This study aimed to evaluate three periods of adaptation, 5, 10 and 15 days, to diets with low or high fiber on digestibility and fecal characteristics. Two diets were used, one containing 0% (0% SH) and one containing 15% soybean hulls (15% SH). Diets were provided to 12 adult dogs. At the end of each period there was total collection of feces to evaluate the apparent digestibility coefficients (ADC), metabolizable energy (ME) and fecal characteristics. We also evaluated diet palatability. There was no effect of the adaptation period on digestibility of diets (P> 0.05). The 15% SH diet reduced ADC of dry matter (DM) and ME, compared to the 0% SH diet (P< 0.05). Fecal consistency of the dogs did not differ among diets and periods (P> 0.05). There was a decrease in fecal pH and ammonia in dogs fed the diet with 15% SH (P< 0.05) after 10 days of feeding (P< 0.05). There was no difference in diet palatability (P> 0.05). Diet adaptation longer than 5 days did not alter the digestibility and most fecal characteristics of dogs, except for fecal ammonia. The inclusion of 15% SH in diet reduces DM digestibility and fecal pH and ammonia in dogs.(AU)

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Objetivou-se avaliar três períodos de adaptação, cinco, 10 e 15 dias, a dietas com baixa ou alta fibra sobre a digestibilidade e características fecais. Foram utilizadas duas dietas, uma contendo 0% de casca de soja (0%CS) e outra com 15% de casca de soja (15%CS), fornecidas a 12 cães adultos. Ao final de cada período, houve coleta total de fezes para avaliação dos coeficientes de digestibilidade aparente (CDA), energia metabolizável (EM) e características fecais. Além disso, foi avaliada a palatabilidade das dietas. Como resultado, não houve efeito do período de adaptação sobre a digestibilidade das dietas (P>0,05). A dieta 15%CS apresentou menor CDA da matéria seca (MS) e EM, em relação à dieta 0%CS (P<0,05). A consistência fecal dos cães não diferiu entre as dietas e os períodos (P>0,05). Houve redução no pH e na amônia fecal dos cães alimentados com a dieta 15%CS (P<0,05), após 10 dias de alimentação. Não houve diferença na palatabilidade das dietas (P>0,05). Períodos de adaptação à dieta superiores a cinco dias não alteram a digestibilidade e a maioria das características fecais de cães, exceto a amônia fecal. A inclusão de 15%CS na dieta reduz a digestibilidade da MS e a EM, bem como o pH e a amônia fecal.(AU)

This study aimed to evaluate three periods of adaptation, 5, 10 and 15 days, to diets with low or high fiber on digestibility and fecal characteristics. Two diets were used, one containing 0% (0% SH) and one containing 15% soybean hulls (15% SH). Diets were provided to 12 adult dogs. At the end of each period there was total collection of feces to evaluate the apparent digestibility coefficients (ADC), metabolizable energy (ME) and fecal characteristics. We also evaluated diet palatability. There was no effect of the adaptation period on digestibility of diets (P> 0.05). The 15% SH diet reduced ADC of dry matter (DM) and ME, compared to the 0% SH diet (P< 0.05). Fecal consistency of the dogs did not differ among diets and periods (P> 0.05). There was a decrease in fecal pH and ammonia in dogs fed the diet with 15% SH (P< 0.05) after 10 days of feeding (P< 0.05). There was no difference in diet palatability (P> 0.05). Diet adaptation longer than 5 days did not alter the digestibility and most fecal characteristics of dogs, except for fecal ammonia. The inclusion of 15% SH in diet reduces DM digestibility and fecal pH and ammonia in dogs.(AU)

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The aim of this study was to measure the nitrogen (N) balance of pigs fed with lysine-limiting diets containing practical levels of wheat bran (WB) or soybean hulls (SH). Twelve pigs with average weights of 57.36±2.01 and 72.68±3.24 kg were used in trials 1 and 2, respectively. In trial 1, treatments were CT1 - control diet and WB - diet with inclusion of 15% WB. In trial 2, CT2 - control diet and SH - diet with inclusion of 6% SH. Fibrous diets increased (P<0.05) the fecal N in 63.54 and 60.55% in relation to CT1 and CT2, respectively. The urinary N was higher (P<0.05) in pigs receiving the WB diet, but was not influenced in the trial with SH. The N retention (NRET) was higher (P>0.05) in pigs ingesting the WB diet; although, when the proportion of NRET was expressed relative to N ingested (NING) there was no difference (P>0.05) between treatments. Inclusion of SH did not affect (P>0.05) the NRET when the result was expressed in absolute or proportional terms. In conclusion, inclusion of practical levels of WB and SH does not significantly affect the metabolic costs involved with nitrogen metabolism. However, only WB was able to contribute to the N balance of pigs, while the relative amount of N added by SH was fully recovered in feces.(AU)

O objetivo deste estudo foi medir o balanço de nitrogênio (N) de suínos alimentados com dietas limitantes em lisina contendo níveis práticos de inclusão de farelo de trigo (FT) ou casca de soja (CS). Foram utilizados 24 suínos machos castrados com peso médio inicial de 57,36±2,01 kg e 72,68±3,24 kg nos experimentos 1 e 2, respectivamente. No experimento 1 os tratamentos foram CT1 - dieta controle, e FT - dieta com inclusão de 15% de FT. No experimento 2 CT2 - dieta controle, e CS - dieta com inclusão de 6% CS. As dietas fibrosas aumentaram (P<0,05) o N fecal em 64,54 e 60,55% em relação a CT1 e CT2, respectivamente. O N urinário foi maior (P<0,05) nos suínos que receberam a dieta FT, mas não foi afetado no experimento com CS. A retenção de N (NRET) foi maior (P<0,05) nos suínos alimentados com a dieta FT, entretanto quando a proporção do NRET foi expressa em relação ao N ingerido (NING) não houve diferença entre os tratamentos (P<0,05). A inclusão de CS não afetou (P<0,05) a NRET quando os resultados foram expressos em termos absolutos ou proporcionais. Em conclusão, a inclusão de níveis práticos de FT ou CS não afeta significativamente o custo metabólico envolvido no metabolismo de N. Entretanto, apenas o FT foi capaz de contribuir para o balanço de N dos suínos, enquanto a quantidade N relativa a adição de CS foi totalmente recuperada nas fezes.(AU)

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Ruminal fermentation produces greenhouse gases involved in global warming. Therefore, the effect of nutrient combinations on methane, carbon dioxide, and biogas production as well as ruminal fermentation kinetics was evaluated in in vitro studies. In total mixed rations, dietary corn grain was partially replaced by two levels of soybean hulls (a highly reusable residue), and a Moringa oleifera extract (a natural extract) at three concentration levels was added. Higher levels of both soybean hulls and M. oleifera extract delayed the initiation of methane production and resulted in a lower methane and carbon dioxide production. Thus, total biogas production was also lower. Replacement of corn grain by soybean hulls tended to lower methane production rates and asymptotic carbon dioxide production, and a delay in biogas and methane formation was observed. Asymptotic biogas and carbon dioxide production, however, were increased. The presence of M. oleifera extract tended to delay methane formation and to decrease methane production rate as well as asymptotic methane production. Higher M. oleifera extract levels decreased asymptotic biogas production with the control and the highest soybean hull levels. In the presence of M. oleifera extract, asymptotic carbon dioxide production was shown to be quadratically increased with the control and lowest soybean hull levels, but quadratically decreased with the highest soybean hull level. With the exception of fermentation pH, the interaction of substrate type and M. oleifera extract level was shown to have an effect on all fermentation parameters. Most fermentation parameters were shown to be higher when replacing corn grain by soybean hulls, including fermentation pH. Thus, the conclusion could be drawn that corn grain replacement by soybean hulls (an agricultural residue) in the presence of M. oleifera extract (a sparing leaf product) could ameliorate greenhouse gas emissions and improve digestion.

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ABSTRACT: The aim of this study was to measure the nitrogen (N) balance of pigs fed with lysine-limiting diets containing practical levels of wheat bran (WB) or soybean hulls (SH). Twelve pigs with average weights of 57.36±2.01 and 72.68±3.24 kg were used in trials 1 and 2, respectively. In trial 1, treatments were CT1 - control diet and WB - diet with inclusion of 15% WB. In trial 2, CT2 - control diet and SH - diet with inclusion of 6% SH. Fibrous diets increased (P<0.05) the fecal N in 63.54 and 60.55% in relation to CT1 and CT2, respectively. The urinary N was higher (P<0.05) in pigs receiving the WB diet, but was not influenced in the trial with SH. The N retention (NRET) was higher (P>0.05) in pigs ingesting the WB diet; although, when the proportion of NRET was expressed relative to N ingested (NING) there was no difference (P>0.05) between treatments. Inclusion of SH did not affect (P>0.05) the NRET when the result was expressed in absolute or proportional terms. In conclusion, inclusion of practical levels of WB and SH does not significantly affect the metabolic costs involved with nitrogen metabolism. However, only WB was able to contribute to the N balance of pigs, while the relative amount of N added by SH was fully recovered in feces.

RESUMO: O objetivo deste estudo foi medir o balanço de nitrogênio (N) de suínos alimentados com dietas limitantes em lisina contendo níveis práticos de inclusão de farelo de trigo (FT) ou casca de soja (CS). Foram utilizados 24 suínos machos castrados com peso médio inicial de 57,36±2,01 kg e 72,68±3,24 kg nos experimentos 1 e 2, respectivamente. No experimento 1 os tratamentos foram CT1 - dieta controle, e FT - dieta com inclusão de 15% de FT. No experimento 2 CT2 - dieta controle, e CS - dieta com inclusão de 6% CS. As dietas fibrosas aumentaram (P<0,05) o N fecal em 64,54 e 60,55% em relação a CT1 e CT2, respectivamente. O N urinário foi maior (P<0,05) nos suínos que receberam a dieta FT, mas não foi afetado no experimento com CS. A retenção de N (NRET) foi maior (P<0,05) nos suínos alimentados com a dieta FT, entretanto quando a proporção do NRET foi expressa em relação ao N ingerido (NING) não houve diferença entre os tratamentos (P<0,05). A inclusão de CS não afetou (P<0,05) a NRET quando os resultados foram expressos em termos absolutos ou proporcionais. Em conclusão, a inclusão de níveis práticos de FT ou CS não afeta significativamente o custo metabólico envolvido no metabolismo de N. Entretanto, apenas o FT foi capaz de contribuir para o balanço de N dos suínos, enquanto a quantidade N relativa a adição de CS foi totalmente recuperada nas fezes.

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We evaluated the characteristics of the non-carcass components of 24 steers (Charolais × Nelore) fed different carbohydrate sources (ground corn, soybean hulls, or wheat bran) in the diet. The animals were 353.2 kg and 22 months of age at early termination, and had been fed forage consisting of sorghum silage at a forage:concentrate ratio of 40.2:59.8. The carbohydrate sources had no effect on the weights of the carcass, vital organs, and blood. The absolute weights of the intestines and rumen fat were higher (P < 0.05) in animals fed corn than in those fed soybean hulls or wheat bran (10.87 vs. 8.89 and 8.87 kg and 5.26 vs. 4.12 and 3.64 kg, respectively). The same pattern was observed when these organs weights were adjusted for empty body weight. The weight of the omasum was highest (P < 0.05) in the wheat bran-fed animals (6.02 vs. 4.70 and 5.49 kg in the corn- and soybean hull-fed animals, respectively). Animals fed soybean hulls had a higher absolute weight of kidney fat (1.6 kg) than steers receiving wheat bran (1.06 kg) or corn (0.79 kg). Wheat bran gave the highest leg weight compared with corn and soybean hulls (2.46 vs. 2.22 and 2.23 kg per 100 kg of empty body weight, respectively).(AU)

Foram avaliadas as características dos componentes não integrantes da carcaça de 24 novilhos castrados, cruza Charolês x Nelore, alimentados com milho, casca de soja ou farelo de trigo como fontes de carboidratos na dieta. Os animais no início da terminação estavam com 353,2 kg e 22 meses de idade e foram alimentados com dieta com relação volumoso:concentrado de 40,2:59,8 sendo o volumoso constituído de silagem de sorgo. Não houve efeito das fontes de carboidratos sobre os rendimentos de carcaça e o peso dos órgãos vitais e de sangue. Os pesos absolutos dos intestinos (10,87; 8,89 e 8,87 kg) e da gordura ruminal (5,26; 4,12 e 3,64 kg) foram mais altos (P < 0,05) nos animais alimentados com milho em relação aos alimentados com casca de soja ou farelo de trigo, respectivamente. Mesmo comportamento ocorreu quando estes órgãos foram ajustados ao peso de corpo vazio. O peso de omaso foi maior (P < 0,05) para a dieta com farelo de trigo em relação ao milho (6,02 versus 4,70 kg), os quais não deferiram da casca de soja (5,49 kg). Animais alimentados com casca de soja apresentaram maior peso absoluto de gordura renal (1,6 kg) em relação aos novilhos que receberam farelo de trigo (1,06 kg) ou milho (0,79 kg). O farelo de trigo proporcionou maior peso de patas em comparação ao milho e a casca de soja, com médias de 2,22; 2,23 e 2,46 kg 100kg-1 de corpo vazio, respectivamente.(AU)

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We evaluated the characteristics of the non-carcass components of 24 steers (Charolais × Nelore) fed different carbohydrate sources (ground corn, soybean hulls, or wheat bran) in the diet. The animals were 353.2 kg and 22 months of age at early termination, and had been fed forage consisting of sorghum silage at a forage:concentrate ratio of 40.2:59.8. The carbohydrate sources had no effect on the weights of the carcass, vital organs, and blood. The absolute weights of the intestines and rumen fat were higher (P < 0.05) in animals fed corn than in those fed soybean hulls or wheat bran (10.87 vs. 8.89 and 8.87 kg and 5.26 vs. 4.12 and 3.64 kg, respectively). The same pattern was observed when these organs weights were adjusted for empty body weight. The weight of the omasum was highest (P < 0.05) in the wheat bran-fed animals (6.02 vs. 4.70 and 5.49 kg in the corn- and soybean hull-fed animals, respectively). Animals fed soybean hulls had a higher absolute weight of kidney fat (1.6 kg) than steers receiving wheat bran (1.06 kg) or corn (0.79 kg). Wheat bran gave the highest leg weight compared with corn and soybean hulls (2.46 vs. 2.22 and 2.23 kg per 100 kg of empty body weight, respectively).

Foram avaliadas as características dos componentes não integrantes da carcaça de 24 novilhos castrados, cruza Charolês x Nelore, alimentados com milho, casca de soja ou farelo de trigo como fontes de carboidratos na dieta. Os animais no início da terminação estavam com 353,2 kg e 22 meses de idade e foram alimentados com dieta com relação volumoso:concentrado de 40,2:59,8 sendo o volumoso constituído de silagem de sorgo. Não houve efeito das fontes de carboidratos sobre os rendimentos de carcaça e o peso dos órgãos vitais e de sangue. Os pesos absolutos dos intestinos (10,87; 8,89 e 8,87 kg) e da gordura ruminal (5,26; 4,12 e 3,64 kg) foram mais altos (P < 0,05) nos animais alimentados com milho em relação aos alimentados com casca de soja ou farelo de trigo, respectivamente. Mesmo comportamento ocorreu quando estes órgãos foram ajustados ao peso de corpo vazio. O peso de omaso foi maior (P < 0,05) para a dieta com farelo de trigo em relação ao milho (6,02 versus 4,70 kg), os quais não deferiram da casca de soja (5,49 kg). Animais alimentados com casca de soja apresentaram maior peso absoluto de gordura renal (1,6 kg) em relação aos novilhos que receberam farelo de trigo (1,06 kg) ou milho (0,79 kg). O farelo de trigo proporcionou maior peso de patas em comparação ao milho e a casca de soja, com médias de 2,22; 2,23 e 2,46 kg 100kg-1 de corpo vazio, respectivamente.

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BACKGROUND: During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cellulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost-efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery. RESULTS: Our work focused on two main topics: growth medium formulation and strain improvement. We evaluated several Brazilian low-cost industrial residues for their potential in cellulase production. Among the solid residues evaluated, soybean hulls were found to display clearly the most desirable characteristics. We engineered a Trichoderma reesei strain to secrete cellulase in the presence of repressing sugars, enabling the use of sugarcane molasses as an additional carbon source. In addition, we added a heterologous ß-glucosidase to improve the performance of the produced enzymes in hydrolysis. Finally, the addition of an invertase gene from Aspegillus niger into our strain allowed it to consume sucrose from sugarcane molasses directly. Preliminary cost analysis showed that the overall process can provide for very low-cost enzyme with good hydrolysis performance on industrially pre-treated sugarcane straw. CONCLUSIONS: In this study, we showed that with relatively few genetic modifications and the right growth medium it is possible to produce considerable amounts of well-performing cellulase at very low cost in Brazil using T. reesei. With further enhancements and optimization, such a system could provide a viable alternative to delivered commercial cellulases.

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Twelve ruminally cannulated steers (401.0 ± 41.5 kg) and 24 mo were used in a replicated arrangement truncated Latin Square with six animals in six treatments and four periods to evaluate the effect of crude glycerine (CG; 80.3% of glycerol) with starch or fiber-based energy ingredients in the concentrate on DMI, DM (DMD) and NDF digestibility (NDFD) and ruminal parameters. Experimental periods were 19 days (14 days for adaptation and 5 days to sampling). Diets were: CO - without CG and corn as ingredient of concentrate; CGC - inclusion of CG (10% of DM) with corn in the concentrate; and CGSH - inclusion of CG (10% of DM) with soybean hulls (SH) in the concentrate. All three diets were offered at low (LC) or high (HC) concentrate level, CL (40 or 60%). Animals fed LC or HC diets had similar DMI, DMD and NDFD. Animals fed diets with CG associated with corn or SH had higher propionate concentrations and lower A:P ratio. Diets with HC increase the propionate but do not affect the NDFD. CG (10% of DM) can be used to replace corn or SH in diets with 40 or 60% of concentrate, without affect NDFD.(AU)

Doze novilhos canulados no rúmen (401,0 ± 41,5 kg) e 24 meses de idade foram usados em delineamento de quadrado latino truncado replicado com seis animais, seis tratamentos e quatro períodos, para avaliar o efeito da glicerina bruta (GB; 80,3% de glicerol) com amido ou ingredientes energéticos a base de fibra no concentrado sobre o CMS, digestibilidade da MS (DMS) e FDN (DFDN) e parâmetros ruminais. O período experimental foi 19 dias (14 dias de adaptação e 5 dias de coleta). As dietas foram: CO sem glicerina bruta e milho como ingrediente do concentrado; GBM GB (10% da MS) com milho no concentrado; GBCS - GB (10% da MS) com casca de soja no concentrado. As três dietas foram ofertadas em baixo (BC) ou alto (AC) teor de concentrado (TC; 40 ou 60%). Animais alimentados com BC ou AC apresentaram similar CMS, DMS e DFDN. Animais alimentados com GB com milho ou CS apresentaram maior concentração de propionato e menor relação A:P. Dietas com AC aumentou a concentração de propionato mas não afetou a DFDN. GB (10% da MS) pode ser usada para substituir o milho ou CS em dietas com 40 ou 60% de concentrado, sem afetar a DFDN.(AU)

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