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Extracellular matrix (ECM)-mimicking microsized cell carriers featuring a semi-isolated chamber facilitate the study of cellular heterogeneity as well as intercellular communication. However, the semiopen shaping of the designated gel mixture remains unattainable with current methods. We report an oil-phase freeze-shrink self-molding mechanism for generating size- and composition-tunable cradle-shaped microgels (microcradles) from water-in-oil droplets. The universality of this shape transition principle is demonstrated with six types of polysaccharides dispersed in a poly(ethylene glycol) diacrylate (PEGDA) or methacrylate gelatin (GelMA) matrix. By doping the microcradles with the major ECM component, hyaluronic acid sodium, we demonstrate a label-free selective culture of CD44 receptor-rich cells and the formation of cell spheroids within 3 days. This cryo-induced cradle-shaping strategy enables the functionalization of microcarriers for selective cell culture, thereby allowing them to be used for intercellular communication, drug delivery, and the construction of structural units for osteogenesis and 3D printing.

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Boron Neutron Capture Therapy is being promoted with the development of accelerator neutron sources, and many new accelerator-based BNCT facilities are being built. In Particle Accelerator Facility project of Sun Yat-sen University, we plan to build a terminal for BNCT research based on an 8 MeV, CW 3 mA proton accelerator. In this paper, we present a beam-shaping assembly for this proton accelerator with such low 24 kW beam power, using composite moderator materials composed of five elements: Mg, Al, F, O, and Li. The calculation result of FLUKA with ENDF/B and JENDL libraries shows that the epithermal neutron beam flux is 1.57×109n/cm2/s with the CW 3 mA proton beam. The fast neutron component and the gamma ray component under free-air condition are 1.49×10-13Gy∙cm2 and 8.12×10-14Gy∙cm2 respectively, in line with IAEA-TECDOC-1223 design recommendations. The thermal analysis shows that the maximum temperature of beryllium target is 706.5 K, and the structure materials of BSA do not melt.

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Four-dimensional (4D) constellations with up to 131 072 points (17 bit/4D-sym) are designed for the first time using geometric shaping. The constellations are optimized in terms of mutual information (MI) and generalized MI (GMI) for the additive white Gaussian noise (AWGN) channel, targeting a forward error correction (FEC) rate of 0.8 at finite signal-to-noise ratios. The presented 15-17 bit constellations are currently the highest-performing constellations in the literature, having a gap to the AWGN capacity as low as 0.17 dB (MI) and 0.45 dB (GMI) at 17 bit/4D-sym. For lower cardinalities, our constellations match or closely approach the performance of previously published optimized constellations. We also show that (GMI-)optimized constellations with a symmetry constraint, optimized for a FEC rate of 0.8, perform nearly identical to their unconstrained counterparts for cardinalities above 8 bit/4D-sym. A symmetry constraint for MI-optimized constellations is shown to have a negative impact in general. The proposed procedure relies on a Monte-Carlo-based approach for evaluating performance and is extendable to other (nonlinear) channels. Stochastic gradient descent is used for the optimization algorithm for which the gradients are computed using automatic differentiation. This article is part of the theme issue 'Celebrating the 15th anniversary of the Royal Society Newton International Fellowship'.

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This study assessed the stress distribution under occlusal forces in mandibular molars after utilizing several nickel-titanium rotary systems and identified potential root fracture patterns through finite element analysis (FEA). Five three-dimensionally printed mandibular molars were used, with one tooth left unshaped and the remaining four shaped using ProTaper Gold (Dentsply, Tulsa Dental Specialties, Tulsa, OK), Reciproc Blue (VDW, Munich, Germany), XP-endo Shaper (FKG Dentaire, La Chaux-de-Fonds, Switzerland), and Hyflex EDM (Coltene/Whaledent, Altst€atten, Switzerland) rotary systems. Subsequently, micro-CT scans were performed on the teeth, and representative FEA models were generated. Two distinct loadings, vertical and oblique, were applied, and stress parameters including von Mises stress, maximum principal stress, and minimum principal stress were recorded and compared across groups. Although stress values in both loading conditions correlated with volume increases post-shaping, the stress distribution patterns indicative of potential fractures were comparable across groups. Stresses under oblique loads were observed to be higher than those under vertical loads. Several rotary systems, based on the volumetric changes they induce in dental hard tissues, may elevate stress values throughout the tooth, leading to root fractures in regions where stress concentration occurs. Conservative root canal shaping leads to a lower overall stress concentration. In mandibular molars, oblique forces have a more destructive effect compared to vertical forces.

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Fluorescent dye films on transparent substrates are essential for OLEDs, flexible displays, X-ray detection, and wireless optical communications. However, their efficiency is often hampered by fluorescence trapping due to total internal reflection (TIR) and waveguiding. This study tackles this longstanding challenge by reconceptualizing the integration of dye films with nanoantenna metasurfaces. Traditional methods involve directly spin-coating films onto c-Si metasurfaces on quartz substrates, resulting in edge luminescence and weak inner signals. We present a straightforward, adjustable approach by integrating dye films on the opposite side of quartz substrates, reaching a 2.5-fold photoluminescence enhancement and improving the uniformity of the emission compared to the conventional methods. These gains stem from redirecting a significant portion of leaked fluorescence light trapped inside the substrate into free space, surpassing TIR conditions through in-plane diffraction orders of the metasurfaces across the full RGB spectrum. Our findings facilitate the design of more efficient luminescent devices.

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We characterize the subsurface thermal degradation of an inert analog of high-explosive molecular crystals (Eu:Y(acac)3(DPEPO)) (EYAD) embedded inside of a plastic bonded explosive simulant using feedback-assisted wavefront shaping-based fluorescence and Raman spectroscopies. This technique utilizes wavefront shaping to focus pump light inside a heterogeneous material onto a target particle, which significantly improves its spectroscopic signature. We find that embedding the EYAD crystals in the heterogeneous polymer results in improved thermal stability, relative to bare crystal measurements, with the crystal remaining fluorescent to >612 K inside of the heterogeneous material, while the bare crystal's fluorescence is fully quenched by 500 K. We hypothesize that this improvement is due to the polymer restricting the effects of EYAD melting, which occurs at 400 K and is the primary mechanism for spectroscopic changes in the temperature range explored.

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Bacterial cellulose (BC) is gathering increased attention due to its remarkable physico-chemical features. The high biocompatibility, hydrophilicity, and mechanical and thermal stability endorse BC as a suitable candidate for biomedical applications. Nonetheless, exploiting BC for tissue regeneration demands three-dimensional, intricately shaped implants, a highly ambitious endeavor. This challenge is addressed here by growing BC within a sacrificial viscoelastic medium consisting of an agarose gel cast inside polydimethylsiloxane (PDMS) molds imprinted with the features of the desired implant. BC produced with and without agarose has been compared through SEM, TGA, FTIR, and XRD, probing the mild impact of the agarose on the BC properties. As a first proof of concept, a PDMS mold shaped as a doll's ear was used to produce a BC perfect replica, even for the smallest features. The second trial comprised a doll face imprinted on a PDMS mold. In that case, the BC production included consecutive deactivation and activation of the aerial oxygen stream. The resulting BC face clone fitted perfectly and conformally with the template doll face, while its rheological properties were comparable to those of collagen. This streamlining concept conveys to the biosynthesized nanocelluloses broader opportunities for more advanced prosthetics and soft tissue engineering uses.

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Compressed ultrafast photography (CUP) can capture irreversible or difficult-to-repeat dynamic scenes at the imaging speed of more than one billion frames per second, which is obtained by compressive sensing-based image reconstruction from a compressed 2D image through the discretization of detector pixels. However, an excessively high data compression ratio in CUP severely degrades the image reconstruction quality, thereby restricting its ability to observe ultrafast dynamic scenes with complex spatial structures. To address this issue, a discrete illumination-based CUP (DI-CUP) with high fidelity is reported. In DI-CUP, the dynamic scenes are loaded into an ultrashort laser pulse train with controllable sub-pulse number and time interval, thus the data compression ratio, as well as the overlap between adjacent frames, is greatly decreased and flexibly controlled through the discretization of dynamic scenes based on laser pulse train illumination, and high-fidelity image reconstruction can be realized within the same observation time window. Furthermore, the superior performance of DI-CUP is verified by observing femtosecond laser-induced ablation dynamics and plasma channel evolution, which are hardly resolved in the spatial structures using conventional CUP. It is anticipated that DI-CUP will be widely and dependably used in the real-time observations of various ultrafast dynamics.

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The introduction of nickel-titanium rotary instruments revolutionized shaping procedures as they were able to produce a well-tapered preparation while reducing operator fatigue. The major drawback of rotary instruments was the high risk of fracture due to bending and torsional stress. Thus, the creation of a glide path has been advocated and recommended by most rotary instrument manufacturers. The aim of the present review is to summarize existing knowledge on glide path preparation and identify areas where further research is needed. The primary goal is to provide a comprehensive overview of the techniques and instruments used in glide path preparation, highlighting their advantages and limitations. The secondary goal is to explore the effect of glide path creation on the overall success of endodontic treatment, particularly in terms of reducing procedural errors and improving treatment outcomes. An online search on PubMed, ScienceDirect, UCLA, and Scopus databases was conducted, and 116 articles were identified. Eligible articles were divided into nine categories based on what they researched and compared. The categories included centering ability and/or root canal transportation, cyclic fatigue resistance, glide path and shaping time, tortional stress resistance, apical extrusion of debris and/or bacteria, defects in dentine walls, file separation, postoperative pain assessment, and scouting ability and performance. Establishing a glide path reduces root canal transportation, especially with rotary methods. Reciprocating and heat-treated files offer higher fatigue resistance and shorter preparation time. Instruments with shorter pitch lengths have greater torsional strength. Preparation and coronal preflaring reduce apical debris and bacteria. Glide paths do not affect dentine microcracks, file separation, or defects but reduce immediate postoperative pain and improve cutting ability. Randomized trials are needed to assess their impact on treatment outcomes.

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Metal-organic frameworks (MOFs) have been extensively considered as one of the most promising types of porous and crystalline organic-inorganic materials, thanks to their large specific surface area, high porosity, tailorable structures and compositions, diverse functionalities, and well-controlled pore/size distribution. However, most developed MOFs are in powder forms, which still have some technical challenges, including abrasion, dustiness, low packing densities, clogging, mass/heat transfer limitation, environmental pollution, and mechanical instability during the packing process, that restrict their applicability in industrial applications. Therefore, in recent years, attention has focused on techniques to convert MOF powders into macroscopic materials like beads, membranes, monoliths, gel/sponges, and nanofibers to overcome these challenges.Three-dimensional (3D) printing technology has achieved much interest because it can produce many high-resolution macroscopic frameworks with complex shapes and geometries from digital models. Therefore, this review summarizes the combination of different 3D printing strategies with MOFs and MOF-based materials for fabricating 3D-printed MOF monoliths and their environmental applications, emphasizing water treatment and gas adsorption/separation applications. Herein, the various strategies for the fabrication of 3D-printed MOF monoliths, such as direct ink writing, seed-assisted in-situ growth, coordination replication from solid precursors, matrix incorporation, selective laser sintering, and digital light processing, are described with the relevant examples. Finally, future directions and challenges of 3D-printed MOF monoliths are also presented to better plan future trajectories in the shaping of MOF materials with improved control over the structure, composition, and textural properties of 3D-printed MOF monoliths.

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Robot navigation has transitioned from avoiding static obstacles to adopting socially aware navigation strategies for coexisting with humans. Consequently, socially aware navigation in dynamic, human-centric environments has gained prominence in the field of robotics. One of the methods for socially aware navigation, the reinforcement learning technique, has fostered its advancement. However, defining appropriate reward functions, particularly in congested environments, holds a significant challenge. These reward functions, crucial for guiding robot actions, necessitate intricate human-crafted design due to their complex nature and inability to be set automatically. The multitude of manually designed reward functions contains issues such as hyperparameter redundancy, imbalance, and inadequate representation of unique object characteristics. To address these challenges, we introduce a transformable Gaussian reward function (TGRF). The TGRF possesses two main features. First, it reduces the burden of tuning by utilizing a small number of hyperparameters that function independently. Second, it enables the application of various reward functions through its transformability. Consequently, it exhibits high performance and accelerated learning rates within the deep reinforcement learning (DRL) framework. We also validated the performance of TGRF through simulations and experiments.

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Objective.The visual perception provided by retinal prostheses is limited by the overlapping current spread of adjacent electrodes. This reduces the spatial resolution attainable with unipolar stimulation. Conversely, simultaneous multipolar stimulation guided by the measured neural responses-neural activity shaping (NAS)-can attenuate excessive spread of excitation allowing for more precise control over the pattern of neural activation. However, defining effective multipolar stimulus patterns is a challenging task. Previous attempts focused on analytical solutions based on an assumed linear nonlinear model of retinal response; an analytical model inversion (AMI) approach. Here, we propose a model-free solution for NAS, using artificial neural networks (ANNs) that could be trained with data acquired from the implant.Approach.Our method consists of two ANNs trained sequentially. The measurement predictor network (MPN) is trained on data from the implant and is used to predict how the retina responds to multipolar stimulation. The stimulus generator network is trained on a large dataset of natural images and uses the trained MPN to determine efficient multipolar stimulus patterns by learning its inverse model. We validate our methodin silicousing a realistic model of retinal response to multipolar stimulation.Main results.We show that our ANN-based NAS approach produces sharper retinal activations than the conventional unipolar stimulation strategy. As a theoretical bench-mark of optimal NAS results, we implemented AMI stimulation by inverting the model used to simulate the retina. Our ANN strategy produced equivalent results to AMI, while not being restricted to any specific type of retina model and being three orders of magnitude more computationally efficient.Significance.Our novel protocol provides a method for efficient and personalized retinal stimulation, which may improve the visual experience and quality of life of retinal prosthesis users.

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The aim was to analyse the influence of an apical ledge on root canal disinfection. Forty-four single-rooted teeth were micro-CT scanned and inoculated with Enterococcus faecalis. In Group S shaping was performed with ProTaper Next (PTN) up to X3 at working length (WL). In Group L an apical ledge was created with K-Files #40 and shaping completed up to PTN X3. NaOCl 5% and EDTA 10% irrigant solutions were alternated. Confocal laser scanning microscope (CLSM) and viability staining were used to analyse the proportions of dead (red) and live (green) bacteria and penetration ability inside dentinal tubules. Data were analysed with the Mann-Whitney test with Bonferroni correction (p < 0.05). In Group L the amount of red fluorescence resulted significantly lower, and penetration ability was decreased in the apical and middle portion (p < 0.05). The presence of an apical ledge may negatively influence the disinfection both in the apical and middle third.

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Dielectric phase gradient metasurfaces have emerged as promising candidates to shrink bulky optical elements to subwavelength thickness scale based on dielectric meta-atoms. These meta-atoms strongly interact with light, thus offering excellent phase manipulation of incident light. However, to fulfill 2π phase control using meta-atoms, the metasurface thickness, to date, is limited to the order of 102 nm. Here, we present the thickness scaling down of phase gradient metasurfaces to <λ/20 by using excitonic van der Waals metasurfaces. High-refractive-index enabled by exciton resonances and symmetry-breaking nanostructures in the patterned layered tungsten disulfide (WS2) corporately enable quasibound states in the continuum in WS2 metasurfaces, which consequently yield complete phase regulation of 2π with the thickness down to 35 nm. To illustrate the concept, we have experimentally demonstrated beam steering, focusing, and holographic display using WS2 metasurfaces. We envision our results unveiling new venues for ultimate thin phase gradient metasurfaces.

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BACKGROUND: Catheter shaping is vital in cerebral aneurysm coil embolization; however, understanding three-dimensional (3D) vascular structures on two-dimensional screens is challenging. Although 3D-printed vascular models are helpful, they demand time, effort, and sterility. This study explores whether mixed-reality (MR) devices displaying 3D computer graphics (3D-CG) can address these issues. METHODS: This study focused on magnetic resonance imaging (MRI) of seven cases of cerebral aneurysms. Head-mounted display (HMD) and spatial reality display (SRD) MR devices were used, and applications for 3D-CG display at a 1:1 scale and a 3D-CG control panel were developed. Catheters shaped using a 3D printer, HMD, and SRD were inserted into hollow models to assess their accessibility and positioning. RESULTS: The concordance rate of the 3D printer and HMD groups in terms of accessibility to the aneurysm was 71.4 %, while that of the 3D printer and SRD group was 85.7 %, and that of the HMD and SRD group was 85.7 %. The concordance rates of positioning in the 3D printer and HMD groups, 3D printer and SRD groups, and HMD and SRD groups were 85.7 %, 85.7 %, and 100 %, respectively. CONCLUSIONS: MR devices facilitate catheter shaping in cerebral aneurysm coil embolization and offer a time-efficient, precise, and sterile alternative to traditional 3D printing methods.

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Colloidal particles of spherical shape are important building blocks for nanotechnological applications. Materials with tailored physical properties can be directly synthesized from self-assembled particles, as is the case for colloidal photonic crystals. In addition, colloidal monolayers and multilayers can be exploited as a mask for the fabrication of complex nanostructures via a colloidal lithography process for applications ranging from optoelectronics to sensing. Several techniques have been adopted to modify the shape of both individual colloidal particles and colloidal masks. Thermal treatment of colloidal particles is an effective route to introduce colloidal particle deformation or to manipulate colloidal masks (i.e. to tune the size of the interstices between colloidal particles) by heating them at elevated temperatures above a certain critical temperature for the particle material. In particular, this type of morphological manipulation based on thermal treatments has been extensively applied to polymer particles. Nonetheless, interesting shaping effects have been observed also in inorganic materials, in particular silica particles. Due to their much less complex implementation and distinctive shaping effects in comparison to dry etching or high energy ion beam irradiation, thermal treatments turn out to be a powerful and competitive tool to induce colloidal particle deformation. In this review, we examine the physicochemical principles and mechanisms of heat-induced shaping as well as its experimental implementation. We also explore its applications, going from tailored masks for colloidal lithography to the fabrication of colloidal assemblies directly useful for their intrinsic optical, thermal and mechanical properties (e.g. thermal switches) and even to the synthesis of supraparticles and anisotropic particles, such as doublets.

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Solar thermal utilization has broad applications in a variety of fields. Currently, maximizing the photo-thermal conversion efficiency remains a research hotspot in this field. The exquisite plant structures in nature have greatly inspired human structural design across many domains. In this work, inspired by the photosynthesis of helical grass, a HM type solar absorber made in graphene-based composite sheets is used for solar thermal conversion. The unique design promoted more effective solar energy into thermal energy through multiple reflections and scattering of solar photons. Notably, the Helical Micropillar (HM) is fabricated using a one-step projection 3D printing process based on a special 3D helical beam. As a result, the solar absorber's absorbance value can reach 0.83 in the 400-2500 nm range, and the surface temperature increased by ≈128.3% relative to the original temperature. The temperature rise rate of the solar absorber reached 22.4 °C min-1, demonstrating the significant potential of the HM in practical applications of solar thermal energy collection and utilization.

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We quantify the chemistry-process-structure-property relationships of a Ti-6Al-4V alloy in which titanium-boron alloy (Ti-B) was added in a functionally graded assembly through a laser-engineered net shaping (LENS) process. The material gradient was made by pre-alloyed powder additions to form an in situ melt of the prescribed alloy concentration. The complex heterogeneous structures arising from the LENS thermal history are completely discussed for the first time, and we introduce a new term called "Borlite", a eutectic structure containing orthorhombic titanium monoboride (TiB) and titanium. The ß-titanium grain size decreased nonlinearly until reaching the minimum when the boron weight fraction reached 0.25%. Similarly, the transformed α-titanium grain size decreased nonlinearly until reaching the minimum level, but the grain size was approximately 2 µm when the boron weight fraction reached 0.6%. Alternatively, the α-titanium grain size increased nonlinearly from 1 to 5 µm as a function of the aluminum concentration increasing from 0% to 6% aluminum by weight and vanadium increasing from 0% to 4% by weight. Finally, the cause-effect relationships related to the creation of unwanted porosity were quantified, which helps in further developing additively manufactured metal alloys.

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Crystalline monolayers prevalent in nature and technology possess elusive elastic properties with important implications in fundamental physics, biology, and nanotechnology. Leveraging the recently discovered shape transitions of oil-in-water emulsion droplets, upon which these droplets adopt cylindrical shapes and elongate, we investigate the elastic characteristics of the crystalline monolayers covering their interfaces. To unravel the conditions governing Euler buckling and Brazier kink formation in these cylindrical tubular interfacial crystals, we strain the elongating cylindrical droplets within confining microfluidic wells. Our experiments unveil a nonclassical relation between the Young's modulus and the bending modulus of these crystals. Intriguingly, this relation varies with the radius of the cylindrical crystal, presenting a nonclassical mechanism for tuning of elasticity in nanotechnology applications.

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White kidney bean (Phaseolus vulgaris L.) extracts can aid weight management by reducing calorie intake from complex carbohydrates through alpha-amylase inhibition. We examined the impact of a proprietary aqueous extract from whole dried white kidney beans standardized by its alpha-amylase inhibitor activity (Phase 2 white kidney bean extract (WKBE)) on weight management in subjects with overweight and moderate obesity. In a randomized, double-blind, placebo-controlled fashion, 81 participants completed the study and ingested either a high dose of Phase 2 (1000 mg, WKBE HIGH), a low dose (700 mg, WKBE LOW), or a matching placebo (microcrystalline cellulose, PLA) three times a day, 30 min before meals, for 12 weeks during a calorie restricted diet. In a dose-dependent manner, Phase 2 significantly reduced body weight, fat mass, BMI, waist, hip and in the WKBE HIGH group thigh circumference. Phase 2 is an effective and safe supplement aiding weight and fat loss. ClinicalTrials.gov identifier NCT02930668.

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