RESUMEN
Aging has become a major global public health challenge. Our previous research showed that R-phycocyanin (R-PC) possessed anti-aging activity. Notably, studies already revealed that gender may affect the responses to the anti-aging drug. Therefore, it is worth investigating whether the anti-aging effects and their underlying molecular mechanisms of R-PC differ between genders. Firstly, R-PC was isolated from porphyra haitanensis and its anti-aging mechanisms were explored using the nature aging male and female drosophila melanogaster as model. Next, the regulation pathway of longevity was analyzed by KEGG pathway analysis. The longevity pathways-associated molecules were also examined to explore anti-aging mechanisms of R-PC. The results showed that R-PC increased AMPK activity, thus enhanced the key regulatory factors of autophagy (Atg1, Atg8, Atg5), and consequently induced autophagy. Hence, the longevity activity of R-PC life was related with AMPK/mTOR/S6K autophagic signaling pathways in aging female drosophila melanogaster. Meanwhile, R-PC significantly down-regulated TNF-α, MMP3, IL-1ß, IL-6, IL-8 expression levels, and the anti-inflammatory and longevity was associated with R-PC-induced regulation of pI3k/AKT/FOXO3 signaling pathway in aging male drosophila melanogaster. These finding showed that R-PC from porphyra haitanensis might exert the anti-aging actions via different mechanisms in male and female drosophila melanogaste.
Asunto(s)
Longevidad , Porphyra , Animales , Femenino , Masculino , Drosophila melanogaster , Ficocianina/farmacología , Proteínas Quinasas Activadas por AMP , Fosfatidilinositol 3-QuinasasRESUMEN
A sex-specific manner in running tasks is considered a potential internal injury risk factor in runners. The current study aimed to investigate the sex differences in running stability in recreational runners during self-preferred speed treadmill running by focusing on a whole-body movement. To this end, principal component analysis (PCA) was applied to kinematic marker data of 22 runners (25.7 ± 3.3 yrs.; 12 females) for decomposing the whole-body movements of all participants into a set of principal movements (PMs), representing different movement synergies forming together to achieve the task goal. Then, the sex effects were tested on three types of PCA-based variables computed for individual PMs: the largest Lyapunov exponent (LyE) as a measure of running variability; the relative standard deviation (rSTD) as a measure of movement structures; and the root mean square (RMS) as a measure of the magnitude of neuromuscular control. The results show that the sex effects are observed in the specific PMs. Specifically, female runners have lower stability (greater LyE) in the mid-stance-phase movements (PM4-5) and greater contribution and control (greater rSTD and RMS) in the swing-phase movement (PM1) than male runners. Knowledge of an inherent sex difference in running stability may benefit sports-related injury prevention and rehabilitation.
RESUMEN
Bisphenol A (BPA) exposure during pregnancy is associated with low fetal weight, particularly in male fetuses. The expression of estrogen-related receptor gamma (ESRRG), a receptor for BPA in the human placenta, is reduced in fetal growth restriction. This study sought to explore whether ESRRG signaling mediates BPA-induced placental dysfunction and determine whether changes in the ESRRG signaling pathway are sex-specific. Placental villous explants from 18 normal term pregnancies were cultured with a range of BPA concentrations (1 nM-1 µM). Baseline BPA concentrations in the placental tissue used for explant culture ranged from 0.04 to 5.1 nM (average 2.3 ±1.9 nM; n = 6). Expression of ESRRG signaling pathway constituents and cell turnover were quantified. BPA (1 µM) increased ESRRG mRNA expression after 24 h in both sexes. ESRRG mRNA and protein expression was increased in female placentas treated with 1 µM BPA for 24 h but was decreased in male placentas treated with 1 nM or 1 µM for 48 h. Levels of 17ß-hydroxysteroid dehydrogenase type 1 (HSD17B1) and placenta specific-1 (PLAC1), genes downstream of ESRRG, were also affected. HSD17B1 mRNA expression was increased in female placentas by 1 µM BPA; however, 1 nM BPA reduced HSD17B1 and PLAC1 expression in male placentas at 48 h. BPA treatment did not affect rates of proliferation, apoptosis, or syncytiotrophoblast differentiation in cultured villous explants. This study has demonstrated that BPA affects the ESRRG signaling pathway in a sex-specific manner in human placentas and a possible biological mechanism to explain the differential effects of BPA exposure on male and female fetuses observed in epidemiological studies.