RESUMEN
Acha grains and sandpaper leaf are commonly used by locals for diabetes and hypertension management, respectively. However, this study sought to produce biscuits from acha-sandpaper leaf composite flour and evaluate their ameliorative potentials in streptozotocin (STZ)/Nω-nitro-l-arginine methylester hydrochloride (l-NAME)-induced hypertensive-diabetic rats. Group I (non-diabetic/normotensive), group II (negative control), group III (positive control) rats were placed on standard drugs, and groups IV-VII rats were placed on formulated biscuits supplemented with 0%, 1.25%, 2.5%, and 3.75% sandpaper leaf flour. The fasting blood glucose (FBG) and blood pressure (BP) of the experimental rats were measured. In addition, the activities of α-amylase, α-glucosidase, and angiotensin-1-converting enzyme (ACE) as well as antioxidant status were evaluated in all the groups and compared. However, the obtained results showed a significant reduction in the FBG and BP and in the activity of α-amylase, α-glucosidase, and ACE in biscuit-fed hypertensive-diabetes rats when compared with group II rats. Meanwhile, antioxidant status in biscuit-fed hypertensive-diabetes rats was enhanced when compared with the untreated hypertensive-diabetic rats. Nevertheless, biscuit with 3.75% sandpaper leaf had better hypertensive-diabetic property than other biscuits which could be linked to bioactive compounds present in the biscuits. PRACTICAL APPLICATIONS: Acha and sandpaper leaf has been used for the management of diabetes and hypertension respectively. However, formulating medicinal biscuits as a diary approach for the management of diabetic and its complication (hypertension) in STZ/l-NAME-induced hypertensive-diabetic rats is highly imperative. Based on our findings, cookies from the acha and sandpaper leaf flour blend exhibits pharmacological effect which could be linked to the presence of numerous bioactive constituents present in the cookies. Thus, the formulated cookies stand as a promising candidate for functional food and further work in this area is needed.
RESUMEN
Sandpaper leaf (Ficus exasperata) is commonly used in folk medicine to manage high blood pressure. However, different methods such as soaking, boiling, hand maceration, and blending have been employed for preparing sandpaper leaf extracts for hypertensive patients. This study sought to investigate and compare the influence of different processing methods on the antihypertensive properties of sandpaper leaf as well as its effect on Fe2+ and sodium nitroprusside- (SNP-) induced lipid peroxidation in rat heart in vitro. Sandpaper leaf extracts were prepared using different methods and the resulting solutions were freeze-dried. Effect of the extracts on angiotensin-1-converting enzymes (ACE) and arginase activity was assessed. Thereafter, antioxidant activities of the extracts were determined using in vitro antioxidant assays, and the total phenolic content and flavonoid content of the extracts were determined. The results revealed that sandpaper leaf extracts from different processing methods inhibited ACE and arginase activity in a dose-dependent manner. However, the soaking method had significantly (p < .05) higher inhibitory effect on angiotensin converting (ACE) and arginase activity than hand maceration, boiling, and blending methods. Sandpaper leaf extracts also exhibited high antioxidant activities as typified by their iron-chelating ability, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical-scavenging and iron-chelating ability as well as inhibition of Fe2+- and SNP-induced lipid peroxidation in rat heart in vitro. Processing methods alter the inhibitory effect of sandpaper leaf extracts on ACE and arginase activities. Thus, inhibition of ACE and arginase coupled with antioxidant properties could offer protective mechanisms against hypertension. However, the soaking method appears to be the most promising among methods considered.
Asunto(s)
Antihipertensivos , Antioxidantes , Ficus , Manipulación de Alimentos/métodos , Extractos Vegetales/farmacología , Hojas de la Planta/química , Inhibidores de la Enzima Convertidora de Angiotensina , Animales , Antihipertensivos/farmacología , Antioxidantes/análisis , Antioxidantes/farmacología , Arginasa/antagonistas & inhibidores , Flavonoides/análisis , Liofilización , Calor , Hipertensión/tratamiento farmacológico , Quelantes del Hierro , Peroxidación de Lípido , Masculino , Medicina Tradicional , Nigeria , Fenoles/análisis , Fitoterapia , Ratas , Ratas Wistar , AguaRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Sandpaper [Ficus exasperata Vahl (Moraceae)] leaf has been reportedly used in folklore for the management/treatment of cardiovascular diseases with little/or no scientific basis for their action. This study sought to investigate the effect of dietary supplementation of sandpaper leaf on angiotensin-I converting enzyme (ACE) activity in hypercholesterolemia as well as the effect of their phenolic extract on this enzyme in vitro. MATERIALS AND METHODS: The phenolic extract was prepared, then, the inhibitory effect of the leaf extract on ACE was determined in vitro. Thereafter, the effect of dietary supplementation of sandpaper leaf on angiotensin-I converting enzyme (ACE) activity in high cholesterol diet fed rats for 14 days was evaluated as well as some biochemical parameters. RESULTS: The result revealed that under in vitro condition, the phenolic extract inhibited ACE (IC50=14.7µg/mL) in a dose-dependent manner (0-10µg/mL). Feeding high cholesterol diets to rats caused a significant (P<0.05) increase in the ACE activity. However, there was a significant (P<0.05) decrease in the ACE activity as a result of supplementation with the sand paper leaves. Furthermore, there was a significant (P<0.05) increase in the plasma lipid profile with a concomitant increase in malondialdehyde (MDA) content in rat liver and heart tissues. However, supplementing the diet with sandpaper leaf (either 10% or 20%) caused a significant (P<0.05) decrease in the plasma total cholesterol (TC), triglyceride (TG), very low density lipoprotein-cholesterol (VLDL-C), and low-density lipoprotein-cholesterol levels (LDL-C), and in MDA content in the tissues. Conversely, supplementation caused a significant (P<0.05) increase in plasma high-density lipoprotein-cholesterol level when compared with the control diet. Reversed phase HPLC analysis of the extract revealed Quercitrin (43.7mg/g), chlorogenic acid (42.8mg/g) and caffeic acid (33.9mg/g) as the major phenolics in the leaf. CONCLUSION: The inhibition of ACE activity and prevention of hypercholesterolemia by sandpaper leaf could be part of the possible mechanism underlying its anti-hypertensive property which could lay credence to its use in folk medicine. However, these activities may be directly/indirectly attributed to the polyphenolics present.