RESUMEN
A new triterpene saponin, 2, 3, 21, 23-tetrahydroxyolean-12-en-28-oic-acid-28-O-ß-D- glucosyl-(2''â1')-ß-D-glucoside (1), was isolated from the n-butanol extract of dried aerial parts of Salvia argentea L. (Lamiaceae) in addition to two known flavonoids, apigenin 7-O-ß-D-glucoside (2), threo-guaiacylglycerol 3-O-[6-O-p-hydroxybenzoyl]-ß-D-glucoside (3), luteolin 7-O-ß-D-glucoside (4), verbascoside (5) and rosmarinic acid (6). The structure elucidation of these compounds was based on analyses of spectroscopic data including 1D-, 2D-NMR and HR-ESI-MS techniques and by comparing their NMR data with those reported in the literature. Compound 6 exhibited the highest antioxidant activity in DPPH assay (IC50<3.00 µg/mL) which was better than the standards BHA, BHT, Trolox and ascorbic acid.
RESUMEN
Five undescribed ursane-type triterpenes, identified as 1ß,3ß,15α-trihydroxy-urs-9(11)-12-diene (1), 1ß,3ß,15α,28-tetrahydroxy-urs-9(11),12-diene (2), 1ß,3ß-dihydroxy-urs-9(11),12-dien-28-al (3), 1ß,3ß,7ß,15α,28-pentahydroxy-urs-12-ene (4), and 1ß,3ß,15α-trihydroxy-urs-11-en-28-al (5), together with five known compounds (6-10), were isolated from the acetone extract of the dried aerial parts of Salvia argentea L. var. aurasiaca (Pomel) Batt. et Trab. (Lamiaceae). Structures were elucidated on the basis of extensive spectroscopic analysis including HR-ESI-MS, 1D- and 2D-NMR techniques and comparison with literature data. The antibacterial evaluation of compounds 1-10 of the acetone extract of the dried aerial parts, in addition to nine compounds (11-19) previously isolated from the extract of the fresh aerial parts, by bioautography on Staphylococcus aureus followed by the determination of MIC values of active compounds by serial dilution technique against five bacteria, revealed that two compounds have an antibacterial effect comparable to antibiotics. The cytotoxic activity evaluation of compounds 1-19 showed that Six compounds exhibited an antiproliferative activity against K562 and HT1080 cells with IC50 values ranging from 30.25 to 70.32⯵M.
Asunto(s)
Antibacterianos/farmacología , Antineoplásicos/farmacología , Salvia/química , Triterpenos/farmacología , Argelia , Antibacterianos/aislamiento & purificación , Antineoplásicos/aislamiento & purificación , Bacterias/efectos de los fármacos , Humanos , Células K562 , Estructura Molecular , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacología , Componentes Aéreos de las Plantas/química , Triterpenos/aislamiento & purificaciónRESUMEN
An ethnobotanical study was carried out in the Saïda region among herbalists to evaluate the use of Salvia argentea (L.), a plant species native from North Africa belonging to the Lamiaceae family. Forty-two herbalists were interviewed individually, aged between 30 and 70 years, all males, 52.38% of them having received a secondary education level and having performing their duties for more than a decade. This study showed that Salvia argentea is used specifically in the treatment of diseases of the respiratory system. The leaves are the most commonly used part, usually in the form of powder and exclusively administered orally. The preliminary results of the physicochemical characterization and the phytochemical screening of the powdered leaves of Salvia argentea attest to their safety and confer them a guarantee of phytotherapeutic quality.
RESUMEN
Ten triterpenoids were isolated from the exudate of Salvia argentea L. var. aurasiaca (Pomel) Batt. & Trab. Their structures were elucidated by 1D and 2D NMR and HRESIMS analyses as 11α-methoxyurs-12-ene-1ß,3ß,15α-triol (1), urs-12-ene-1ß,3ß,11α,15α-tetraol (2), 11α-methoxyurs-12-ene-1ß,3ß-diol (3), 1ß,3ß,15α-trihydroxy-11α-methoxyurs-12-en-28-al (4), 1ß,3ß,15α-trihydroxyurs-12-en-28-al (5), urs-12-ene-1ß,3ß,15α,28-tetraol (6), 11α-methoxyurs-12-ene-1ß,3ß,28-triol (7), 13ß,28-epoxyurs-12-ene-1ß,3ß-diol (8), urs-12-ene-3ß,7ß,15α,28-tetraol (9) and olean-12-ene-3ß,7ß,15α,28-tetraol (10). A chemotaxonomic survey of the triterpenoids of Salvia species show that triterpenoids from the exudate of S. argentea var. aurasiaca (Pomel) Batt. & Trab., provide some features, such as hydroxylation at C-15, and hydroxylation at C-7. On this basis, S. argentea L. var. aurasiaca (Pomel) Batt. & Trab., is quite different from S. argentea L.