RESUMEN

Acinetobacter baumannii poses a significant health threat because of its frequent implications in hospital outbreaks and multidrug resistance (MDR). Here, we studied four A. baumannii isolates recovered during a hospital outbreak of severe or fatal cases to elucidate their diversity and factors contributing to their increased virulence and antibiotic resistance. The isolates were identified using MALDI-ToF and characterized using comparative genomics, PCR, and antimicrobial susceptibility tests. They were classified as ST126 and exhibited fewer than five chromosomal single-nucleotide variants and the same extrachromosomal content, indicating that they are a single strain (A. baumannii AB01). A. baumannii AB01 showed an MDR phenotype that could be linked to the carriage of parC and gyrA mutations, efflux transporters, aminoglycoside resistance genes, a class C beta-lactamase, and three carbapenemases, some of which are encoded on a 72 kb plasmid. ST126 is infrequent and has not been reported in Latin America, and our genomic data indicate a plausible origin for A. baumannii AB01 within the Pan Pacific region.

Asunto(s)

Infecciones por Acinetobacter , Acinetobacter baumannii , Proteínas Bacterianas , Brotes de Enfermedades , Farmacorresistencia Bacteriana Múltiple , Pruebas de Sensibilidad Microbiana , Plásmidos , beta-Lactamasas , beta-Lactamasas/genética , Humanos , Infecciones por Acinetobacter/microbiología , Infecciones por Acinetobacter/epidemiología , Acinetobacter baumannii/genética , Acinetobacter baumannii/efectos de los fármacos , Acinetobacter baumannii/enzimología , Acinetobacter baumannii/aislamiento & purificación , Farmacorresistencia Bacteriana Múltiple/genética , Plásmidos/genética , Proteínas Bacterianas/genética , Antibacterianos/farmacología , Masculino , Femenino , Infección Hospitalaria/microbiología , Infección Hospitalaria/epidemiología , Persona de Mediana Edad