RESUMEN

Field pea seeds have long been recognized as valuable feed ingredients for animal diets, due to their high-quality protein and starch digestibility. However, the chemical composition of pea cultivars can vary across different growing locations, consequently impacting their nutrient profiles. This study employs untargeted metabolomics in conjunction with the quantification of fatty acids and amino acids to explore the influence of three different growing locations in Spain (namely Andalusia, Aragon and Asturias), on the nutritional characteristics of seeds of various pea cultivars. Significant interactions between cultivar and environment were observed, with 121 metabolites distinguishing pea profiles. Lipids, lipid-like molecules, phenylpropanoids, polyketides, carbohydrates, and amino acids were the most affected metabolites. Fatty acid profiles varied across locations, with higher C16:0, C18:0, and 18:1 n-9 concentration in Aragón, while C18:2 n-6 predominated in Asturias and C18:3 n-3 in Andalusia. Amino acid content was also location-dependent, with higher levels in Asturias. These findings underscore the impact of environmental factors on pea metabolite profiles and emphasize the importance of selecting pea cultivars based on specific locations and animal requirements. Enhanced collaboration between research and industry is crucial for optimizing pea cultivation for animal feed production.

Asunto(s)

RESUMEN

In this work, ACs were originated from two different bio-waste sources of Date and Jujube seeds (DS and JS). The influence of the precursor type as well as KOH chemical activator ratio on the structural properties and CO2 adsorption performances of synthesized ACs were assessed. Impact of pre-treatment of raw material via functionalization with urea on the performance of prepared adsorbents was also evaluated. Functionalized DS-based AC possessed the highest surface area and largest micropore volume equal to 864 m2/g and 0.33 cm3/g, respectively. CO2 adsorption behavior of ACs was experimentally evaluated via TGA at different adsorption temperatures of 25 and 50 °C and CO2 concentrations of 10 and 90 vol% under atmospheric pressure. Based on the TGA results, functionalized and non-functionalized DS-prepared ACs with KOH: biochar weight ratio of 2:1, demonstrated great CO2 capture capacity up to 1.3 and 1.2 mmol/g, respectively under realistic condition of 10 vol% CO2 and 25 °C. The urea-nitrogenation and KOH-activation as economical and simple approaches sensitively assisted preparation of a novel and promising N-doped porous AC from bio-waste resources which can be exploited for superior CO2 capture applications.

RESUMEN

Ginkgo biloba seeds have been used as a traditional Chinese medicine for hundreds of years to treat diseases such as cervicitis, cough, asthma and other lung diseases. As a novel form, the dispensing granules (GSDG) of Ginkgo biloba seeds have been widely employed in clinic. However, its chemical profiling is not yet clear, which has restricted in-depth research in many fields. In this study, a high performance liquid chromatography coupled with quardrupole time-of-flight mass spectrometry method was used for the component characteration with the help of accurate molecular weights, fragmentation pathways, reported data, literatures and even some reference standards. Furthermore, in multiple-reaction monitoring mode, a high performance liquid chromatography coupled with quadrupole linear ion trap mass spectrometry method was developed and applied for simultaneous determination of the bioactive phytochemicals. As a result, a total of 56 components in GSDG were identified including 12 amino acids, 9 organic acids, 6 nucleosides and nucleobases, 6 flavonoids, 5 vitamins, 5 terpenoid lactones, 4 carbohydrates and 9 other compounds As for quantitative analysis, glutamic acid, asparatic acid, histidine, ginkgolide A, ginkgolide B, ginkgolide C, ginkgolide J, eucomic acid, N-(N-glucopyranosyl)-indoleacetylaspartate and N-(N-glucopyranosyl)-indoleacetylglutamate were selected as the analytes for quanlity marker of GSDG. After necessary validation tests, the developed quantitative method was successfully put into use for 10 batches of GSDG. In all batches, N-(N-glucopyranosyl)-indoleacetylaspartate was the richest phytochemical with the amount of 17.3-25.7 mg/g while ginkgolide J (0.0197-0.0335 mg/g) was determined to be the poorest. The study is supposed to exhibit a comprehensive chemical profiling and to provide some strong basis for preparation technology, quality control and even for action mechanism of GSDG, this novel form of Chinese medicine.

RESUMEN

Dyes are among the toxic contaminants that significantly impact water ecosystems. A biomaterial prepared from Zizyphus Spina-Christi seed (ZSCS) to remove methylene blue (MB) and methyl violet (MV) from an aqueous solution was investigated. Several techniques have been used, including FTIR, SEM, EDX, XPS, and TGA, to characterize the physical and chemical properties of ZSCS. The effect of various parameters such as pH, adsorbent dosage, contact time, temperature, and initial dye concentration on the adsorption process were studied. The ZSCS adsorbent showed efficient MB and MV dye adsorption with Langmuir adsorption capacity of 666.66 and 476.19 mg/g, respectively, at experimental condition [(pH = 6; time = 30 min; T = 45 °C, dye concentration: 500 mg/L, and adsorbent dose = 0.6 g/L for MB and 1 g/L for MV dye)]. Kinetic and isotherm models were applied to fit the experimental outcomes. The result showed that ZSCS showed an ultrafast absorption process with a high removal efficiency of MB and MV within 5 min indicating its effective adsorption properties. The Langmuir isotherm model was the most suitable model for describing the adsorption of MB and MV dyes on ZSCS. The pseudo-second-order model kinetic fits better to MB and MV adsorption onto ZSCS than other models, suggesting that the adsorption mechanism followed chemisorption. Our results could offer an efficient cost-effective approach for dye removal from wastewater.

RESUMEN

Chromatin immunoprecipitation and sequencing (vs ChIP-seq) is an essential tool for epigenetic and molecular genetic studies. Although being routinely used, ChIP-seq is expensive, requires grams of plant materials, and is challenging for samples that enrich fatty acids such as seeds. Here, we developed an Ultrasensitive Plant ChIP-seq (UP-ChIP) method based on native ChIP-seq combined with Tn5 tagmentation-based library construction strategy. UP-ChIP is generally applicable for profiling both histone modification and Pol II in a wide range of plant samples, such as a single Arabidopsis seedling, a few Arabidopsis seeds, and sorted nuclei. Compared with conventional ChIP-seq, UP-ChIP is much less labor intensive and only consumes 1 µg of antibody and 10 µl of Protein-A/G conjugated beads for each IP and can work effectively with the amount of starting material down to a few milligrams. By performing UP-ChIP in various conditions and genotypes, we showed that UP-ChIP is highly reliable, sensitive, and quantitative for studying histone modifications. Detailed UP-ChIP protocol is provided. We recommend UP-ChIP as an alternative to traditional ChIP-seq for profiling histone modifications and Pol II, offering the advantages of reduced labor intensity, decreased costs, and low-sample input.

RESUMEN

This study explored natural deep eutectic solvent-based polyphenolic extract from date fruit seed as a functional and bioactive compound in chitosan-poly(vinyl)alcohol (CSPVA) films. Various concentrations of the extract (1.5 %, 2 %, 2.5 %, and 3 %) were added to study the effect on the film's bioactive, physicochemical, mechanical and structural properties. The extract increased the total phenolic content (0.01 to 0.16 mg GAE/mL), and antioxidant activities determined via 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay (1.45 to 5.53 mmol GAE/mL), and ferric ion reducing antioxidant power assay (0.12 to 2.4 µmol TE/mL) assays. It also enhanced antibacterial activities against Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli. The extract was also successful in increasing the elongation at break (45.51 % to 58.16 %) and thickness (0.10 to 0.19 mm) while reducing tensile strength (11.18 MPa to 3.02 MPa) and Young's modulus (24.5 MPa to 5.7 MPa). UV-shielding ability, opacity, water vapor permeability (3.7 to 7.6 × 10-10 g/m.s.Pa) and solubility (53.7 % to 73.9 %) also increased. CSPVA films with 3 % DSP preserved white shrimps better than cling film by reducing quality deterioration (i.e., color, lipid oxidation, and bacterial population) after 4 days of refrigeration. These findings suggest that CSPVA films enriched with green-extracted date seed polyphenolic compounds hold significant potential for sustainable food packaging.

RESUMEN

Maturity and drying treatment are important factors affecting the processing characteristics of lotus seeds and its starch. This study aimed to investigate the effect of maturity (from low to high-M-1, M-2, M-3, M-4) on far-infrared drying kinetics of lotus seeds, and on the variation of structure, gelation and digestive properties of lotus seed starch (LSS) before and after drying. As the maturity increased, the drying time reduced from 5.8 to 1.0 h. The reduction of drying time was correlated with the decrease of initial moisture content, the increase of water freedom and the destruction of tissue structure during ripening. The increased maturity and drying process altered the multiscale structure of LSS, including an increase in amylose content, disruption of the short-range structure, and a decrease in relative crystallinity and molecular weight. The viscosity, pasting temperature and enthalpy of LSS decreased during ripening, and drying treatment caused the further decrease. The digestibility of LSS increased during ripening and drying. Lotus seeds at M-4 would be optimal for obtaining shorter drying time, lower pasting temperature and enthalpy, and higher digestibility. This study provided theoretical guidance for achieving effective drying process and screening LSS with suitable processing properties through maturity sorting.

Asunto(s)

RESUMEN

Abrus precatorius is an ornamental plant that belongs to the Leguminoceae family. It contains toxalbumin, named abrin, in all of its parts. However, the seeds are more toxic when consumed in crushed form. Deaths due to abrus seed poisoning are rare. We are reporting a case of suicidal ingestion of crushed abrus precatorius seeds by a 37-year-old female. She presented to the hospital with complaints of multiple episodes of vomiting and diarrhea. She was resuscitated with fluid boluses, followed by a stomach wash and activated charcoal, and referred to our tertiary hospital, where she presented with giddiness, abdominal pain, bloody diarrhea, and epigastric tenderness. On subsequent days, she developed altered sensorium, renal failure, and electrolyte imbalance. She was managed conservatively. Blood investigation revealed elevated leucocyte count, increased urea and creatinine levels, and elevated liver enzymes. She died five days after ingesting the seeds. On autopsy examination, the brain and lungs were congested and edematous. The peritoneal cavity contained around 500mL of straw-colored fluid. Petechial hemorrhages were present over the lungs, heart, and liver surfaces. The mucosa of the gastrointestinal tract was hemorrhagic, the liver showed steatosis, and the kidneys showed congestion and obscuration of the corticomedullary junction. Histopathologically, the lungs showed mild congestion with alveolar edema, the liver showed necrosis with steatosis, and the kidney showed acute tubular necrosis. The toxicology screening was positive for abrin. This case highlights not only the rare fatality following abrus precatorius poisoning but also the toxic nature of this plant.

RESUMEN

In the search for new antibacterial agents from plants, two known compounds (rotenoid and chalcone) were isolated from Tephrosia vogelii Hook.f. (seeds), namely tephrosin (1), and obovatachalcone (2). The compounds were isolated using vacuum liquid chromatography and radial chromatography. The structures of isolates were determined by 1D and 2D NMR. Both compounds were evaluated for their antibacterial properties against the four pathogenic bacterial strains Staphylococcus aureus S33 R4, Bacillus subtilis subsp. subtilis str. 168, Escherichia coli TISTR 780, and Salmonella typhi Ty 21a, using the microdilution method to determine MIC and MBC. The results showed that tephrosin (1) had moderate antibacterial activity against E. coli with MIC values of 25 µg/mL and MBC values of 100 µg/mL. However, obovatachalcone (2) showed a weak antibacterial activity.

RESUMEN

The aim of this study was to produce and to characterize craft beer fermented by immobilized yeast cells with the addition of Prokupac grape pomace seed powder (2.5% and 5%), to obtain a beer enriched with phenolic compounds and improved sensory characteristics. The immobilization of the yeast cells was performed by electrostatic extrusion, while the obtained calcium alginate beads were characterized by light and scanning electron microscopy. Phenolic and hop-derived bitter compounds in beer with or without grape pomace seed powder (GS) phenolics were identified using UHPLC Q-ToF MS. The results indicated that GS adjunct significantly shortened the fermentation process of wort and increased the content of phenolic compounds, especially ellagic acid, flavan-3-ols and pro(antho)cyanidins in the final products compared to the control beer. A total of twenty (iso)-α-acids and one prenylflavonoid were identified, although their levels were significantly lower in beers with GS phenolics compared to the control beer. Beers with GS phenolics showed good antioxidant properties as measured by the reduction of ferric ions (FRP) and the scavenging of ABTS•+ and DPPH• radicals. The concentration of immobilized viable yeast cells was higher than 1 × 108 CFU/g wet mass after each fermentation without destroying the beads, indicating that they can be reused for the repeated fermentation of wort. Beers produced with 5% GS added to the wort exhibited the best sensory properties (acidity, astringency, bitterness intensity, mouthfeel, aftertaste and taste), and highest overall acceptability by the panelists. The results showed that grape pomace seed powder present a promising adjunct for the production of innovative craft beer with good sensory properties and improved functionality.

RESUMEN

Opium poppy is a plant used in both the pharmaceutical and food industries. Substances found on the surface of dry poppy seeds belong to the group of opium alkaloids. However, the presence of these substances in food products poses a risk to consumer health, which is why new permissible levels for both substances in poppy seeds and derivative products have been introduced in Regulation (EU) 2023/915. This research aimed to analyze the content of all six opium alkaloids in poppy seeds provided directly by producers as well as those available on the local market in Poland. The research confirmed the presence of morphine in all examined poppy seed samples. The alkaloid content ranged from 12.46 to 102.86 mg/kg for seeds purchased in local markets and from 1.1 to 110.1 mg/kg for seeds obtained directly from producers. Both groups showed similar levels of morphine content as well as other OAs, which significantly exceeded the permissible limit of 20 mg/kg set by the European Commission (EU) 2023/915. These results indicate that the presence of morphine and other opium alkaloids in poppy seeds exceeds permissible levels, posing a serious health issue and necessitating further research and improvement in processing methods.

RESUMEN

PFASs are a variety of ecologically persistent compounds of anthropogenic origin loosely included in many industrial products. In these, the carbon chain can be fully (perfluoroalkyl substances) or partially (polyfluoroalkyl substances) fluorinated. Their ubiquitous presence in many environmental compartments over the years and their long-lasting nature have given rise to concerns about the possible adverse effects of PFASs on ecosystems and human health. Among a number of remediation technologies, adsorption has been demonstrated to be a manageable and cost-effective method for the removal of PFASs in aqueous media. This study tested two novel and eco-friendly adsorbents (pinewood and date seeds biochar) on six different PFASs (PFOS, GenX, PFHxA, PFOA, PFDA, and PFTeDA). Batch sorption tests (24 h) were carried out to evaluate the removal efficiency of each PFAS substance in relation to the two biochars. All samples of liquid phase were analyzed by a developed and then a well-established method: (i) pre-treatment (centrifugation and filtration) and (ii) determination by high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS). The results evidenced a comparable adsorption capacity in both materials but greater in the long-chain PFASs. Such findings may lead to a promising path towards the use of waste-origin materials in the PFAS remediation field.

RESUMEN

Botanical varieties of hemp differ in chemical composition, plant morphology, agronomy, and industrial suitability. Hemp is popular for cultivation for the production of cannabinoid oil, fiber production, biomass, etc. The fertilization process is one of the most important factors affecting the plant, both its condition and chemical composition. So far, research has been carried out proving that hemp is a valuable source of, among others: fatty acids, amino acids, acids, vitamins, numerous micro- and macroelements, and antioxidant compounds. In this experiment, it was decided to check the possibility of harvesting hemp panicles twice in one year. The purpose of this treatment is to use one plant to produce cannabidiol oil and grain. The main aim of the research was to determine bioactive compounds in hemp seeds and to determine whether the cultivation method affects their content and quantity. Based on the research conducted, it was observed that hemp can be grown in two directions at the same time and harvested twice because its health-promoting properties do not lose their value. It was found that regardless of whether hemp is grown solely for seeds or to obtain essential oils and then seeds, the type of fertilization does not affect the content of phenolic acids (e.g., syringic acid: 69.69-75.14 µg/100 g, vanillic acid: 1.47-1.63 µg/100 g). Based on the conducted research, it was found that essential oils can be obtained from one plant in the summer and seeds from Henola hemp cultivation in the autumn, because such a treatment does not affect the content of the discussed compounds.

Asunto(s)

RESUMEN

Wild soybeans retain many substances significantly reduced or lost in cultivars during domestication. This study utilized LC-MS to analyze metabolites in the seed coats and embryos of wild and cultivated soybeans. 866 and 815 metabolites were identified in the seed extracts of both soybean types, with 35 and 10 significantly differing metabolites in the seed coat and embryos, respectively. The upregulated metabolites in wild soybeans are linked to plant defense, stress responses, and nitrogen cycling. MALDI-MSI results further elucidated the distribution of these differential metabolites in the cotyledons, hypocotyls, and radicles. In addition to their role in physiological processes like growth and response to environmental stimuli, the prevalent terpenoids, lipids, and flavonoids present in wild soybeans exhibit beneficial bioactivities, including anti-inflammatory, antibacterial, anticancer, and cardiovascular disease prevention properties. These findings underscore the potential of wild soybeans as a valuable resource for enhancing the nutritional and ecological adaptability of cultivated soybeans.

RESUMEN

Alkaline ferri/ferro-cyanide-based flow batteries are well suited for energy storage because of their features of high electrochemical activity, good kinetics and low material cost. However, they suffer from low energy density and poor temperature adaptability. The ferri/ferro-cyanide catholyte exhibits low solubility (~0.4 M at 25 °C) in NaOH- or KOH-based supporting electrolyte and can easily form precipitates below room temperature. Here we report a lithium-based supporting electrolyte that significantly enhances the solubility of ferrocyanide. The use of LiOH intensifies the ion-dipole interaction between water molecules and solutes and cripples polarization among ferrocyanide ions. Thus, we have achieved a ferrocyanide-based catholyte of 1.7 M at 25 °C and of 0.8 M at -10 °C. A zinc-ferricyanide flow battery based on the lithium-based supporting electrolyte demonstrates a steady charge energy of ~72 Wh L-1catholyte at 25 °C for ~4200 cycles (~4200 hours). Furthermore, it remains stable for ~800 cycles (~800 hours) at -10 °C.

RESUMEN

Sweet cherry pits, date seeds, and grape seeds are abundant fruit by-products in the Mediterranean region. Assessing their antioxidant capacity is crucial for their valorization. Grape and date seeds exhibited higher concentrations of total phenolic and flavonoid contents, and significant antioxidant capacity. Epicatechin was the main flavonoid in sweet cherry pits and date seeds (29-85 mg/g), while vanillic acid was the predominant phenolic acid across all by-products (5-23 mg/g). However, some sweet cherry pit varieties exceeded Maximum Residue Levels (MRL) for five pesticides, while grape seeds contained thirteen fungicide residues, all below MRL. Ochratoxin A was detected in one date seed but below the limit of quantification. Additionally, grape seeds showed an Al content of approximately 130 mg/kg, along with levels of As, Cd, and Pb. Date seeds exhibited high potential for food and pharmaceutical applications, pending evaluation for chemical contaminants.

RESUMEN

Various seeds, including sea buckthorn (Hippophae rhamnoides L.) seeds, are sources of different bioactive compounds. They can show anti-inflammatory, hypoglycemic, anti-hyperlipidemic, antibacterial, antioxidant, or other biological properties in in vitro and in vivo models. Our preliminary in vitro results have demonstrated that the extracts from raw (no thermal processing) and roasted (thermally processed) sea buckthorn seeds have antioxidant potential and anticoagulant activity. However, it was unclear which compounds were responsible for these properties. Therefore, in continuation of our previous study, the extracts were fractionated by C18 chromatography. Phytochemical analysis of three fractions (a, b, and c) from raw sea buckthorn seeds and four fractions (d, e, f, and g) from roasted sea buckthorn seeds were performed. Several in vitro assays were also conducted to determine the antioxidant and procoagulant/anticoagulant potential of the fractions and two of their major constituents-isorhamnetin 3-O-ß-glucoside7-O-α-rhamnoside and serotonin. LC-MS analyses showed that serotonin is the dominant constituent of fractions c and f, which was tentatively identified on the basis of its HRMS and UV spectra. Moreover, fractions c and f, as well as b and e, contained different B-type proanthocyanidins. Fractions b and e consisted mainly of numerous glycosides of kaempferol, quercetin, and isorhamnetin. The results of oxidative stress assays (measurements of protein carbonylation, lipid peroxidation, and thiol groups oxidation) showed that out of all the tested fractions, fraction g (isolated from roasted seeds and containing mainly dihexoses, and serotonin) demonstrated the strongest antioxidant properties.

Asunto(s)

RESUMEN

There is concern that agrobiodiversity is being irreversibly eroded in the face of agricultural industrialization. While academic and policy debates stress loss of landraces, little attention has been paid to evaluating how agricultural knowledge systems endure in response to broader social-ecological changes (i.e., "system's resilience"). For being resilient, agricultural knowledge systems should incorporate new information (modern seed varieties) whilst maintaining its traditional components (landraces) and functions. However, the loss or continuing utilization of landraces may be influenced by several social-ecological filters, which are processes that selectively remove varieties according to their phenotype, local uses, or value. We examined the resilience of agricultural knowledge systems in the southern Andes. These systems include the knowledge of landraces and modern varieties by campesinos and lifestyle migrants. We further assessed the association of social-ecological filters with the knowledge of agrobiodiversity. Over four years (2018-2022), we used mixed-methods including semi-structured interviews with gardener experts and conducted knowledge exercises of seed varieties and surveys of gardeners (n = 132). We assessed the association of 'knowledge score on varieties' (general, landraces, and modern) with a priori-defined social-ecological filters. Gardeners with more proficient knowledge of landraces were more knowledgeable of modern varieties too. The general knowledge of agrobiodiversity and the knowledge of landraces, but not of modern varieties, was higher for campesinos than migrants. The main seed source of gardeners, the participation in seed exchanges, gardeners' origin, and gardeners' age were the social-ecological filters that influenced gardeners' knowledge of agrobiodiversity. We highlight that social-ecological, small-scale farming systems, are being resilient when they have the capacity of incorporating new information (knowledge of modern varieties) whilst maintaining their identity (knowledge of landraces) without undergoing a major shift in their basic structures and functions in this Important Agricultural Heritage Site and Global Biodiversity Hotspot, and beyond.

RESUMEN

Lithium (Li) metal is considered a hopeful anode for next-generation Li-ion batteries thanks to its ultra-high theoretical specific capacity, extra-low theoretical density, and low negative potential. However, the uncontrolled growth of Li dendrites and volume fluctuation during plating/stripping processes severely hamper its commercial application. Herein, ZnS seeds sealed in N, P, S co-doped carbon hollow rhombic dodecahedra (ZnS@NPS-C HRD) is fabricated as a superlithiophilic host for Li metal anodes (LMAs) to solve the above problems. In addition, the Li nucleation and deposition mechanism on ZnS@NPS-C HRD is investigated by in situ optical microscopy, ex-situ X-ray diffraction, scanning electron microscopy, and theoretical calculations. Owing to the synergistic strategy of ZnS seeds-inducing nucleation and Li-limited growth, the as-prepared composite exhibits stability for 300 cycles in asymmetric cells and a long lifespan over 1100 h in symmetric cells. Moreover, the ZnS@NPS-C HRD@Li|LiFePO4 full cell demonstrates a reversible capacity of 100.91 mAh g-1 after 400 cycles at 1 C.

RESUMEN

INTRODUCTION: The discovery and development of new phytomedicines can be greatly aided by plants because of their tremendous therapeutic benefits, efficiency, cost-effectiveness, lack of side effects, and cheaper therapies. In this regard, Quercus baloot, generally known as oak, is used in folkloric medicine for treating and preventing various human disorders, including diabetes. AIM: For this purpose, the present study aimed to evaluate crude methanolic extract and various fractions of Quercus baloot for antihyperlipidemic and antihyperglycemic potential followed by the analysis of active compounds. METHOD: The hypoglycemic and hypolipidemic activity was evaluated in Swiss male Albino mice by administering an oral dose of 150-300 mg/kg of Q. baloot extracts in alloxan induced diabetic mice for 14 days. RESULTS: The results revealed that crude methanolic extract at a dose of 300 mg/kg exhibited a significant reduction in the blood glucose level (198.50 ± 1.99 mg/dl) at day 14 and the same treatment significantly increased the body weight (31.26 ± 0.27g) at day 14 in comparison to the control group. Moreover, the biochemical parameters were investigated which presented an increase in high-density lipids (HDL) (30.33 ± 0.33 mg/dl), whereas Low-Density Lipids (LDL) showed a significant decrease (105.66 ± 0.26 mg/dl). Additionally, triglyceride levels 104.83 ± 0.70 mg/dl, and total cholesterol 185.50 ± 0.76 mg/dl are significantly decreased. In serum biochemical analysis creatinine and hepatic enzyme markers, like serum glutamate pyruvate transaminase (32.00 ± 0.36 U/mg), serum glutamate oxaloacetate transaminase (34.33 ± 0.61 U/mg), and alkaline phosphatase (157.00 ± 0.73 U/mg), were significantly reduced by the crude methanolic extract at a dose of 300 mg/kg as compared to the control group. The antioxidant enzymes like Superoxide dismutase (4.57 ± 0.011), peroxidases dismutase (6.53 ± 0.014, and catalase (8.38 ± 0.014) at a dosage of 300 mg/kg of methanolic extract exhibited a significant increase. The histopathological study of the diabetic heart, liver, and pancreas showed substantial restoration of damaged tissues in the methanolic extract 150 and 300 mg/kg treated group, which supports the effectiveness of Q. baloot seeds. The gas chromatography-mass spectrometry analysis of methanolic extract identified 10 antidiabetic active compounds in the Q. baloot seeds, validating the antihyperglycemic activity. Thus, methanolic crude extract at the doses 150 and 300 mg/kg of Q. baloot showed significant antihyperlipidemic and antihyperglycemic activities, which validate the folkloric utilization of Q. baloot as a remedy in diabetes. CONCLUSION: In conclusion, the 300 mg/kg methanolic extract of Q. baloot has notable hypoglycemic and hypolipidemic potential, supporting the plant's traditional medicinal usage in the treatment of diabetes and its complications. Further studies are needed for the purification, characterization, and structural clarification of bioactive compounds.