RESUMEN

Objective: To study the chemical constituents from the ethanol extract fraction herbs of Qibai Pingfei Granule (QPG), the difference of mixed decoction and separated decoction of ethanol extract fraction herbs in QPG. Methods: UPLC-Q-TOF-MS was used as the analytic method. The information on quasi-molecular ion, fragment ions, retention time, and references were synthetically analyzed to confirm the constituents in the ethanol extract fraction herbs of QPG. Principal components analysis was applied to process the acquired data, and marked differences between mixed decoction and separated decoction in the ethanol extract fraction herbs of QPG. Results: Twenty-two compounds were identified, and four compounds were tentatively identified among 26 ions. In addition, twelve compounds were determined as markers to distinguish the mixed decoction from the separated decoction of the ethanol extract fraction herbs in QPG. Conclusion: The findings obtained from the study can provide the useful information for the determination of bioactive substances, the perfection of quality standard, and the preparation technology of QPG.