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D-Allulose (D-psicose) is a rare sugar and a C-3 epimer of D-fructose. D-Allulose has been reported to have several health benefits via its alteration of both glucose and lipid metabolism. It was previously reported that D-allulose alters the hepatic metabolomic profile. Although the kidneys are crucial organs in metabolic regulation, the effects of D-allulose on renal metabolism have not yet been established. Therefore, this study was designed to capture the overall metabolic response in the kidneys to D-allulose. This was done by providing an AIN-93G diet to Wistar rats, with or without 3 % D-allulose, for four weeks. Renal tissue and blood samples were collected after a 3-hour fasting for evaluation of the renal metabolic profile and their related plasma parameters. D-Allulose increased renal weight without changes in the plasma indices associated with reduced renal function. Metabolic profiling identified a total of 264 peaks. As the contribution rate was too low in the principal component analysis results of the metabolic profiling results, we evaluated the metabolites that were significantly different between two groups and identified 23 up-regulated and 26 down-regulated metabolites in the D-allulose group. D-Allulose also had significant influence on several metabolites involved in glucose metabolism, amino acid metabolism, and purine metabolism. Moreover, the levels of trimethylamine N-oxide and symmetric dimethylarginine, which are associated with several diseases such as chronic kidney disease and cardiovascular disease decreased following D-allulose diets. This study showed that D-allulose affects the renal metabolic profile, and our findings will help elucidate the function of D-allulose.

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D-psicose-3-epimerase (DPEase), a key enzyme for D-psicose production, has been successfully expressed in Escherichia coli with high yield. However, intracellular expression results in high downstream processing costs and greater risk of lipopolysaccharide (LPS) contamination during cell disruption. The secretory expression of DPEase could minimize the number of purification steps and prevent LPS contamination, but achieving the secretion expression of DPEase in E. coli is challenging and has not been reported due to certain limitations. This study addresses these challenges by enhancing the secretion of DPEase in E. coli through computational predictions and structural analyses. Signal peptide prediction identified PelB as the most effective signal peptide for DPEase localization and enhanced solubility. Supplementary strategies included the addition of 0.1% (v/v) Triton X-100 to promote protein secretion, resulting in higher extracellular DPEase (0.5 unit/mL). Low-temperature expression (20 °C) mitigated the formation of inclusion bodies, thus enhancing DPEase solubility. Our findings highlight the pivotal role of signal peptide selection in modulating DPEase solubility and activity, offering valuable insights for protein expression and secretion studies, especially for rare sugar production. Ongoing exploration of alternative signal peptides and refinement of secretion strategies promise further enhancement in enzyme secretion efficiency and process safety, paving the way for broader applications in biotechnology.

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BACKGROUND: D-psicose 3-epimerase (DPEase) is a potential catalytic enzyme for D-psicose production. D-psicose, also known as D-allulose, is a low-calorie sweetener that has gained considerable attention as a healthy alternative sweetener due to its notable physicochemical properties. This research focused on an in-depth investigation of the expression of the constructed DPEase gene from Agrobacterium tumefaciens in Escherichia coli for D-psicose synthesis. Experimentally, this research created the recombinant enzyme, explored the optimization of gene expression systems and protein purification strategies, investigated the enzymatic characterization, and then optimized the D-psicose production. Finally, the produced D-psicose syrup underwent acute toxicity evaluation to provide scientific evidence supporting its safety. RESULTS: The optimization of DPEase expression involved the utilization of Mn2+ as a cofactor, fine-tuning isopropyl ß-D-1-thiogalactopyranoside induction, and controlling the induction temperature. The purification process was strategically designed by a nickel column and an elution buffer containing 200 mM imidazole, resulting in purified DPEase with a notable 21.03-fold increase in specific activity compared to the crude extract. The optimum D-psicose conversion conditions were at pH 7.5 and 55 °C with a final concentration of 10 mM Mn2+ addition using purified DPEase to achieve the highest D-psicose concentration of 5.60% (w/v) using 25% (w/v) of fructose concentration with a conversion rate of 22.42%. Kinetic parameters of the purified DPEase were Vmax and Km values of 28.01 mM/min and 110 mM, respectively, which demonstrated the high substrate affinity and efficiency of DPEase conversion by the binding site of the fructose-DPEase-Mn2+ structure. Strategies for maintaining stability of DPEase activity were glycerol addition and storage at -20 °C. Based on the results from the acute toxicity study, there was no toxicity to rats, supporting the safety of the mixed D-fructose-D-psicose syrup produced using recombinant DPEase. CONCLUSIONS: These findings have direct and practical implications for the industrial-scale production of D-psicose, a valuable rare sugar with a broad range of applications in the food and pharmaceutical industries. This research should advance the understanding of DPEase biocatalysis and offers a roadmap for the successful scale-up production of rare sugars, opening new avenues for their utilization in various industrial processes.

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Di-d-psicose anhydride (DPA), derived from functional rare saccharide as d-psicose, is investigated for its strong chelating ability. Methylglyoxal (MGO), an important precursor of advanced glycation end-products (AGEs), promotes obesity, and causes complications such as diabetic nephropathy. On mesangial cells, DPA can substantially reduce the negative effects of MGO. DPA effectively trapping MGO in mesangial cells. The bonding properties of the DPA-MGO adduct were discussed by mass spectrometry and nuclear magnetic resonance (NMR). The NMR spectra of the DPA-MGO adduct provide evidence for chelation bonding. The inhibition of AGE formation and the mass spectrometry results of the DPA-MGO adduct indicate that DPA can scavenge MGO at a molar ratio of 1:1. DPA suppressed 330 % of the up-regulated receptor for an AGEs protein expression to a normal level and restored the suppressed glyoxalase 1 level to 86 % of the normal group. This research provides important evidence and theoretical basis for the development of AGE inhibitors derived from rare saccharide.

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This assessment addresses a food enzyme preparation consisting of the immobilised non-viable cells of the non-genetically modified bacterium identified by the applicant (Samyang Corporation) as Microbacterium foliorum strain SYG27B. This strain produces the enzyme D-psicose 3-epimerase (EC 5.1.3.30). The food enzyme preparation is used for the isomerisation of fructose to produce the speciality carbohydrate D-allulose (synonym D-psicose). Since the hazard identification and characterisation could not be made and the identity of the production organism could not be established, the Panel was unable to complete the assessment of this food enzyme preparation containing D-psicose 3-epimerase.

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Este artigo apresenta uma discussão sobre as repercussões do modo de funcionamento marcado pela pulsão oral como um elemento decisivo para que alcancemos as bases mais primordiais da psicose maníaco- depressiva. Entendemos que este estudo também pode auxiliar na elucidação da presença de estados melancoliformes e maniformes em configurações neuróticas de maior gravidade, ou ainda, em psicoses não desencadeadas. Nosso recorte temático será desenvolvido a partir do referencial freudiano, conferindo um espaço significativo às contribuições de Karl Abraham. Também contará com a exposição de novas colaborações da psicanálise pós-freudiana, com enfoque nos impactos da voracidade pulsional na vida humana e as consequências psicopatológicas desse tipo de fixação libidinal. Por fim, buscaremos alinhavar pontos levantados com o diferencial das teorizações de Jacques Lacan, resgatando sucintamente princípios de sua teoria da clínica como a relação ao grande Outro, a lógica fantasmática, o objeto a e o significante paterno.

Cet article présente une discussion sur les répercussions du mode de fonctionnement subjectif marqué par la pulsion orale comme un élément fondamental pour comprendre les bases les plus primordiales de la psychose maniaco-dépressive. Nous comprenons que cette étude peut également aider à élucider la présence d'états en format mélancolique et maniaque dans des configurations névrotiques de gravité plus élevée, ou encore, dans des psychoses non déclenchées. Notre découpage thématique sera développé à partir de la théorie freudienne, accordant un espace significatif aux contributions de Karl Abraham. Il inclura également l'exposition de nouvelles collaborations de la psychanalyse post-freudienne, en mettant l'accent sur les conséquences de la voracité pulsionnelle dans la vie humaine et les conséquences psychopathologiques de ce genre de fixation libidinale. Enfin, nous chercherons à relier les points relevés avec la spécificité des théorisations de Jacques Lacan, reprenant brièvement les principes de sa théorie clinique tels que la relation au grand Autre, la logique du fantasme, l'objet a et le signifiant paternel.

This article presents a discussion about the repercussions of the predominance of the oral drive in subjective functioning as a decisive element to reach the primordial bases of manic-depressive psychosis. We understand that this study can also help enlighten the presence of melancholic and manic states in more severe neurotic configurations, or even in untriggered psychoses. Our thematic framework will be based on Freudian theory, giving significant space to the contributions of Karl Abraham. It will also include the exposition of new contributions from post-Freudian psychoanalysis, focusing on the impacts of drive eagerness in human life and the psychopathological consequences of this type of fixation of libido. Finally, we will seek to connect the issues raised with the uniqueness of Jacques Lacan's theorizations, briefly revisiting the principles of his clinical theory such as the relation to the Other, the phantasmatic logic, the object a, and the fatherly signifier.

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Rare sugars are known for their ability to suppress postprandial blood glucose levels. Therefore, oligosaccharides and disaccharides derived from rare sugars could potentially serve as functional sweeteners. A disaccharide [α-d-allopyranosyl-(1→2)-ß-d-psicofuranoside] mimicking sucrose was synthesized from rare monosaccharides D-allose and D-psicose. Glycosylation using the intermolecular aglycon delivery (IAD) method was employed to selectively form 1,2-cis α-glycosidic linkages of the allopyranose residues. Moreover, ß-selective psicofuranosylation was performed using a psicofuranosyl acceptor with 1,3,4,6-tetra-O-benzoyl groups. This is the first report on the synthesis of non-reducing disaccharides comprising only rare d-sugars by IAD using protected ketose as a unique acceptor; additionally, this approach is expected to be applicable to the synthesis of functional sweeteners.

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Rice husk, rich carbon content, is an agricultural waste produced globally at an amount of 120 million tons annually, and it has high potential as a biorefinery feedstock. Herein, we investigated the feasibility of producing various products as D-psicose, bioethanol and lactic acid from rice husk (RH) through a biorefinery process. Alkali-hydrogen peroxide-acetic acid pretreatment of RH effectively removed lignin and silica, resulting in enzymatic hydrolysis yield of approximately 86.3% under optimal hydrolysis conditions. By using xylose isomerase as well as D-psicose-3-epimerase with borate, glucose present in the RH hydrolysate was converted into D-psicose with a 40.6% conversion yield in the presence of borate. Furthermore, bioethanol (85.4%) and lactic acid (92.5%) were successfully produced from the RH hydrolysate. This study confirmed the high potential of RH as a biorefinery feedstock, and it is expected that various platform chemicals and value-added products can be produced using RH.

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Resumo: Desde o início do desenvolvimento de sua teoria, Freud observara que os psicóticos não respondiam ao tratamento psicanalítico, levando à ideia de que à psicose corresponderia um mecanismo de constituição diferente. Aos poucos, a transferência passou a ser alvo da investigação que conduzisse à descoberta de um modo a lidar com a psicose. Mas as descobertas freudianas foram insuficientes para se estabelecer um laço transferencial satisfatório para os psicóticos. Com Jacques Lacan, alguns conceitos freudianos sofreram modificação, com a criação de operadores próprios, surgindo a erotomania, consequência estrutural e lógica do vínculo entre analista e psicótico. Sendo assim, este artigo busca demonstrar uma clínica possível, sob a perspectiva psicanalítica, para o tratamento da psicose, a partir do estabelecimento do laço transferencial, por meio do manejo apropriado do gozo, que promova estabilização e consiga mitigar o sofrimento, como saída possível para o problema da indicação do tratamento psicanalítico aos psicóticos.

Résumé : Au début de ses développements théoriques, Freud a observé que les psychotiques ne répondaient pas au traitement psychanalytique, indiquant qu'un mécanisme de formation différent correspondrait à la psychose. Progressivement, le transfert est devenu la cible de la découverte d'une manière pour faire face à la psychose. Jacques Lacan a modifié certains concepts freudiens pour crée des opérateurs propres et a inventé l'érotomanie, la conséquence structurelle et logique de la relation entre analyste et psychotique. Cet article propose une clinique possible, dans la perspective psychanalytique, comme solution possible au traitement de la psychose à partir de l'établissement du transfert par le biais du maniement approprié de la jouissance qui conduise à la stabilisation.

Abstract: Early in his theoretical developments, Freud noticed that psychotics did not respond to the psychoanalytic treatment, suggesting that psychosis would correspond to a different constitution mechanism. Gradually, transference became the target of investigation for discovering a way to tackle psychosis. However, Freud's findings were insufficient to solve the issue of establishing a satisfactory transference bond with psychotics. Jacques Lacan modified some Freudian concepts to create his own operators and coined erotomania, the structural and logical consequence of the bond between analyst and psychotic. This article proposes a possible clinic, from a psychoanalytic perspective, as a possible treatment route for psychosis by establishing a transference bond via the appropriate management of jouissance, which promotes stabilization.

Resumen: Desde el comienzo del desarrollo de su teoría, Freud observó que los psicóticos no respondían al tratamiento psicoanalítico, lo que dio lugar a la idea de que la psicosis correspondía a un mecanismo de formación diferente. Gradualmente, la transferencia se convirtió en el objetivo de la investigación que podría conducir al descubrimiento de una manera peculiar para hacer frente a la psicosis. En el estudio de Jacques Lacan, algunos conceptos freudianos se han modificado con la creación de operadores propios, y emergió la erotomanía como consecuencia estructural y lógica de la relación entre el analista y el psicótico. Así, este artículo busca demostrar una posible clínica desde una perspectiva psicoanalítica para el tratamiento de la psicosis, basada en el establecimiento del vínculo transferencial, a través del adecuado manejo del goce que promueva la estabilización y logre mitigar el sufrimiento, como una posible salida al problema de indicar tratamiento psicoanalítico a los psicóticos.

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Este artigo tem por objetivo analisar o aspecto ético da fenomenologia das psicoses estabelecida pela Daseinsanalyse psiquiátrica de Ludwig Binswanger, a partir do modo como Merleau-Ponty concebe a experiência de outrem enquanto intercorporeidade. Inicialmente se apresenta a relação entre psiquiatria e fenomenologia, considerando a perspectiva de Binswanger sobre o problema da objetividade científica no campo da clínica e sua busca por desenvolver uma abordagem fundada na intersubjetividade. Ao final, procurou-se realizar alguns apontamentos sobre o sentido possível da aproximação entre fenomenologia e psiquiatria, de modo a tornar possível uma fenomenologia das psicoses que se constitua pelo reconhecimento da alteridade.

Resumos This article aims to analyze the ethical aspect of the phenomenology of psychoses established by Ludwig Binswanger's psychiatric Daseinsanalyse, based on the way Merleau-Ponty conceives the experience of others as intercorporeity. Initially, the relationship between psychiatry and phenomenology is presented, considering Binswanger's perspective on the problem of scientific objectivity in the clinical field and his quest to develop an approach based on intersubjectivity. Finally, we tried to make some notes about the possible meaning of the approximation between phenomenology and psychiatry, in order to make possible a phenomenology of psychoses that is constituted by the recognition of alterity.

Cet article analyse la dimension éthique de la phénoménologie des psychoses établie par la Daseinsanalyse psychiatrique de Ludwig Binswanger, basée sur la manière dont Merleau-Ponty conçoit l'expérience d'autrui comme intercorporéité. Dans un premier temps, nous présentons la relation entre la psychiatrie et la phénoménologie, en considérant la perspective de Binswanger sur le problème de l'objectivité scientifique dans le domaine clinique et sa quête pour développer une approche basée sur l'intersubjectivité. Enfin, nous essayons quelques remarques sur le sens possible du rapprochement entre phénoménologie et psychiatrie, afin de rendre possible une phénoménologie des psychoses qui soit constituée par la reconnaissance de l'altérité.

Este artículo tiene como objetivo realizar un análisis del aspecto ético de la fenomenología de las psicosis establecida por el Daseinsanalyse psiquiátrico de Ludwig Binswanger, a partir de cómo Merleau-Ponty concibe la experiencia del otro como intercorporeidad. Inicialmente, se presenta la relación entre psiquiatría y fenomenología teniendo en cuenta la perspectiva de Binswanger sobre el problema de la objetividad científica en el campo clínico y su búsqueda por desarrollar un enfoque basado en la intersubjetividad. Por último, se intenta hacer algunos apuntes sobre el posible significado de la aproximación entre fenomenología y psiquiatría para posibilitar una fenomenología de las psicosis que se constituya en el reconocimiento de la alteridad.

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Resumo Enquadramento: As psicoses são caraterizadas por distorções do pensamento, da perceção e emoções inadequadas. Em contexto forense, cabe ao enfermeiro especialista em saúde mental e psiquiátrica prevenir comportamentos violentos através de intervenções psicoterapêuticas. Objetivo: Caraterizar a situação clínica dos participantes relativamente às variáveis: funcionamento social, atividades socialmente úteis, relações pessoais e sociais, comportamento perturbador e agressivo, assertividade e sinais precoces de agressão; avaliar a eficácia de um programa de intervenção para treino de competências sociais e diminuição de preditores de violência. Metodologia: Estudo pré-experimental, com delineamento de pré e pós-teste com grupo único, cuja amostra foram 15 doentes internados em serviços de psiquiatria forense. Resultados: O programa demonstrou-se eficaz, observando-se mudanças estatisticamente significativas a nível das relações pessoais, dos comportamentos perturbadores e agressivos e dos sinais precoces de agressão. Conclusão: O treino de habilidades sociais e de autocontrolo traduziu-se na melhoria dos indicadores clínicos e na reabilitação psicossocial dos participantes, contribuindo para uma melhor (re)integração na família e na comunidade.

Abstract Background: Psychosis is characterized by distortions of thinking and perception, as well as inappropriate emotions. In forensic settings, psychiatric mental health nurses are responsible for preventing violent behaviors through psychotherapeutic interventions. Objective: To characterize participants' clinical situation based on the following variables: social performance, socially useful activities, personal and social relationships, disturbing and aggressive behavior, assertiveness, and early signs of aggression; and To assess the effectiveness of an intervention program for training social skills and reducing predictors of violence. Methodology: Pre-experimental study with a one-group pretest-posttest design. The sample consisted of 15 patients admitted to forensic psychiatric wards. Results: The intervention proved effective, with statistically significant changes in personal relationships, disturbing and aggressive behaviors, and early signs of aggression. Conclusion: Training social skills and self-control improved participants' clinical indicators and psychosocial rehabilitation and contributed to better (re)integrating them into their families and communities.

Resumen Marco contextual: La psicosis se caracteriza por distorsiones del pensamiento, de la percepción y de las emociones inapropiadas. En el contexto forense, es responsabilidad del enfermero especialista en salud mental y psiquiátrica prevenir las conductas violentas mediante intervenciones psicoterapéuticas. Objetivo: Caracterizar la situación clínica de los participantes en cuanto a las siguientes variables: funcionamiento social, actividades socialmente útiles, relaciones personales y sociales, comportamiento disruptivo y agresivo, asertividad y signos tempranos de agresión. Evaluar la eficacia de un programa de intervención para entrenar las competencias sociales y reducir los predictores de violencia. Metodología: Estudio preexperimental, con diseño antes y después de la prueba con un único grupo, cuya muestra estaba compuesta por 15 pacientes ingresados en los servicios de psiquiatría forense. Resultados: El programa demostró ser eficaz y se observaron cambios estadísticamente significativos en las relaciones personales, el comportamiento disruptivo y agresivo, y los primeros signos de agresión. Conclusión: El entrenamiento de las competencias sociales y del autocontrol se tradujo en la mejora de los indicadores clínicos y en la rehabilitación psicosocial de los participantes, lo que contribuyó a una mejor (re)integración en la familia y la comunidad.

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D-Allose is a rare cis-caprose with a wide range of physiological functions, which has a wide range of applications in medicine, food, and other industries. L-Rhamnose isomerase (L-Rhi) is the earliest enzyme found to catalyze the production of D-allose from D-psicose. This catalyst has a high conversion rate, but its specificity for substrates is limited; thus, it cannot fulfill the requirements of industrial production of D-allose. In this study, L-Rhi derived from Bacillus subtilis was employed as the research subject, and D-psicose as the conversion substrate. Two mutant libraries were constructed through alanine scanning, saturation mutation, and rational design based on the analysis of the secondary structure, tertiary structure, and interactions with ligands of the enzyme. The yield of D-allose produced by these mutants was assessed; it was found that the conversion rate of mutant D325M to D-allose was increased by 55.73 %, and the D325S improved by 15.34 %, while mutant W184H increased by 10.37 % at 55 °C, respectively. According to modeling analysis, manganese (Mn2+) had no significant effect on the production of D-psicose from D-psicose by L-Rhi. The results of molecular dynamics simulation demonstrated that the mutants W184H, D325M, and D325S had more stable protein structures while binding with the substrate D-psicose, as evidenced by its root mean square deviation (RMSD), root mean square fluctuation (RMSF), and binding free energy values. It was more conducive to binding D-psicose and facilitating its conversion to D-allose, providing the basis for the production of D-allose.

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Introducción: el delirio constituye uno de los síntomas más complejos y severos de la psicosis. Uno de los problemas más fundamentales que enfrenta la investigación en el campo de la neuropsiquiatría tiene que ver con el desafío de producir teorías explicativas para la producción de este tipo de estado mental, a lo que se denomina problema etiológico de los delirios psicóticos. Desarrollo: se analizan críticamente las principales alternativas dentro de la neuropsiquiatría contemporánea al problema etiológico en el contexto de la esquizofrenia. Conclusión: el análisis indica que las tres teorías fundamentales que conviven en la actualidad poseen problemas para avanzar en el desafío de explicar la etiología de los delirios. Por lo anterior, se propone la idea de que, a la luz del estado del debate reciente, la hibridación teórica podría perfilarse como el mejor candidato metodológico para generar progreso real en la disciplina

Introduction: Delusions constitute one of the most complex and severe symptoms of psychosis. One of the most fundamental problems within current research in neuropsychiatry has to do with the challenge of producing explanatory theories of the aetiology of the phenomenon. I call this the etiological problem of psychotic delusions. Development: The main alternatives to the etiological problem in schizophrenia in current neuropsychiatry are critically assessed. Conclusion: The three current co-existing approaches possess a number of problems to make real progress in the etiological debate. For this reason, I suggest that, in light of the current state of the art, theoretical hybridization could become the best methodologi- cal candidate to make progress within the target debate

Introdução: o delírio é um dos sintomas mais complexos e graves da psicose. Um dos problemas mais fundamentais enfrentados atualmente pelas pesquisas no campo da neuropsiquiatria diz respeito ao desafio de produzir teorias explicativas para a produção desse tipo de estado mental, que chamo de o problema etiológico dos delírios psicóticos. Desenvolvimento: são analisadas criticamente as principais alternativas da neuropsiquiatria contemporânea ao problema etiológico no contexto da esquizofrenia. Conclusão: a análise indica que as três teorias fundamentais que coexistem atualmente têm problemas para avançar no desafio de explicar a etiologia dos delírios. Portanto, propõe-se a ideia de que, à luz do estado atual do debate, a hibridação teórica poderia surgir como o melhor candidato metodológico para gerar um progresso real na disciplina.

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D-Psicose is a rare, low-calorie sugar that is found in limited quantities in national products. Recently, D-psicose has gained considerable attention due to its potential applications in the food, nutraceutical, and pharmaceutical industries. In this study, a novel D-psicose 3-epimerase (a group of ketose 3-epimerase) from an extremely halophilic, anaerobic bacterium, Iocasia fonsfrigidae strain SP3-1 (IfDPEase), was cloned, expressed in Escherichia coli, and characterized. Unlike other ketose 3-epimerase members, IfDPEase shows reversible epimerization only for D-fructose and D-psicose at the C-3 position but not for D-tagatose, most likely because the Gly218 and Cys6 at the substrate-binding subsites of IfDPEase, which are involved in interactions at the O-1 and O-6 positions of D-fructose, respectively, differ from those of other 3-epimerases. Under optimum conditions (5 µM IfDPEase, 1 mM Mn2+, 50 °C, and pH 7.5), 36.1% of D-psicose was obtained from 10 mg/mL D-fructose. The IfDPEase is highly active against D-fructose under NaCl concentrations of up to 500 mM, possibly due to the excessive negative charges of acidic amino acid residues (aspartic and glutamic acids), which are localized on the surface of the halophilic enzyme. These negative charges may protect the enzyme from Na+ ions from the environment and result in the lowest pI value compared to those of other 3-epimerase members. Moreover, without adjusting any ingredients, IfDPEase could improve coconut water quality by converting D-fructose into D-psicose with a yield of 26.8%. Therefore, IfDPEase is an attractive alternative to enhancing the quality of fructose-containing foods.

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O presente artigo busca investigar o campo da psicose e sua relação com fenômenos relativos à espiritualidade. Ao longo da história da psiquiatria, a loucura foi destituída de sua dimensão espiritual com uma compreensão científica dos fenômenos; porém, a nosografia médica também operou uma redução do fenômeno apenas à dimensão descritiva. Neste artigo foi realizada uma revisão teórica narrativa sobre a temática de espiritualidade, emergência espiritual, processos de renovação, psicose e tratamentos para além da medicação. A revisão de literatura sinaliza que as crises psicóticas não necessariamente indicam o desenvolvimento de uma esquizofrenia e que essa crise também pode carregar um potencial transformador. Por fim, reflete-se como a dimensão religiosa pode ser acolhida em um atendimento psicológico ético e sua importância para o acolhimento de crises psicóticas.

The present article intends to investigate the field of psychosis and its relation to phenomena related to spirituality. Through the history of psychiatry, madness was stripped from its spiritual dimension with the scientific compreehension of phenomena. By the otherside, the medical nosography also operated a reduction of the phenomena only to a descriptive dimension. The authors developed a theoretical narrative revision about the themes of spirituality, spiritual emergency, renewal process, psychosis and the treatments beyond medication. The literature review indicate that psychotic crisis do not necessarily indicate a development of schizophrenia and that this crisis can also carry a transformative potential. Thus, it is discussed how that the religious dimension can be approached in an ethical psychological session and its importance on dealing with a psychotic crisis.

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O trabalho realizado e que vai aqui apresentado consiste em uma apresentação do percurso empreendido por Jacques Lacan ([1955-56] 1988) no Seminário sobre As Psicoses. A proposta de chamar o nosso percurso de "diálogos" baseia-se na leitura de outros textos e notícias que reportam ao tema, ajudando-nos a melhor compreendê-lo. Não é um percurso simples e tampouco óbvio. A escrita de Lacan, ou mesmo a estrutura psicótica, abre continuamente outras portas pelas quais pode o observador perscrutar, embora não lhe seja permitido adentrar com suas ansiosas pretensões interpretativas, plenas de significantes simbólicos. Contente-se, pois, o clínico, com a função de secretário do delírio, compreendendo-o não simplesmente como uma defesa, mas como uma outra forma de se organizar diante de um outro ­ imagem de si e que rejeita habitar o complexo mundo da linguagem.

The work carried out and which will be presented here consists of a presentation of the journey undertaken by Jacques Lacan ([1955-56] 1988) at the Seminar on The Psychoses. The proposal to call our journey "dialogues" is based on reading other texts and news that report on the topic, helping us to better understand it. It is not a simple or obvious route. Lacan's writing, or even the psychotic structure, continually opens other doors through which the observer can peer, although he is not allowed to enter with his anxious interpretative pretensions, full of symbolic signifiers. Therefore, the clinician is content, with the role of secretary of the delusion, understanding it not simply as a defense, but as another way of organizing himself in front of another - an image of himself that rejects inhabiting the complex world of language.

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In this paper, a three-electrode electrochemical detection system was designed. Platinum electrode was used as the counter electrode, saturated KCl electrode as reference electrode, and copper film material as working electrode, respectively. Cyclic voltammetry (CV) and chronoamperometry (i-t) methods were used for d-psicose scanning. CV results indicated that d-psicose presented the oxidation-reduction reacting procedure on the surface of copper film electrode. Testing parameters optimization was conducted using CV scanning in different scanning rates. d-psicose quantitative determination model was developed by i-t scanning results. The sensitivity was9419.1A×cm-2·mol/L, the detection limit was1.04311×10-8mol/L. The proposed method has some advantages including high sensitivity, low detection line, and fast response speed. Negative control testing results by using glucose, sucrose, and NaCl solutions demonstrated that the proposed method had good selectivity.

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SCOPE: d-allulose is a low-calorie rare sugar. It has been reported that d-allulose supplementation significantly inhibits diet-induced hepatic fat accumulation. However, the underlying molecular mechanisms remain unclear. This study elucidates the mechanism underlying the suppressive effect of d-allulose on hepatic fat accumulation in terms of miRNA regulation. METHODS AND RESULTS: Male C57BL/6 mice are divided into three experimental groups-normal diet and distilled water (CC group), high-fat diet (HFD) and distilled water (HC group), and HFD and 5% d-allulose solution (HA group)-and fed the respective diets for 8 weeks. Weight gain is significantly lower in the HA group than that in the HC group, although the caloric intake is the same in both. Histological analysis of liver tissues reveals excessive lipid accumulation in the HC group; this is greatly attenuated in the HA group. Real-time PCR and western blot analyses demonstrate that, compared to the HC group, the HA group exhibits decreased hepatic PPARγ and CD36 expression. Hepatic miR-130 expression levels are higher in the HA group than those in the CC and HC groups. CONCLUSIONS: These results indicate that miRNA changes associated with PPARγ may underlie the suppression of hepatic lipid accumulation induced by d-allulose intake.

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D-Psicose, a new-generation sugar substitute, has been enzymatically synthesized through D-fructose isomerization. However, isomerization often causes low yields due to unfavorable thermodynamic equilibria, which limited its further industrial application. In this study, we present a redox-driven multi-enzyme cascade, two-step biotransformation system to produce D-psicose from D-fructose. Compared to D-fructose isomerization, this method has a maximized theoretical conversion rate of 100%. D-Psicose-3-epimerase from Clostridiales (CBDPE), ribitol 2-dehydrogenase from Providencia alcalifaciens (PRDH), and formate dehydrogenase from Starkeya (SFDH) were co-expressed in Escherichia coli in the first step to produce D-allitol from D-fructose. Afterward, NADH oxidase from Streptococcus pyogenes (SPNOX), and ribitol 2-dehydrogenase from Rubrivivax sp. (RSRDH) were co-expressed in E. coli to oxidize D-allitol into D-psicose in the second step. The two-step biotransformation system was optimized to maximize the D-fructose-to-D-psicose conversion rate (up to 90%), corresponding to a concentration of 450 mM. This study suggests that this redox-driven multi-enzyme cascade strategy through a sugar-to-alcohol-to-sugar pathway has the advantage of great application for enhanced production of D-psicose and other rare sugars.

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Este artigo trata de uma experiência institucional de estágio no Institut Médico Pédagogique Notre Dame de la Sagesse, também conhecido como Le Courtil. O Courtil é uma instituição belga que acolhe crianças e jovens com impasses no laço social. A partir do caso clínico de uma garota de oito anos, discute-se a importância dos objetos para a invenção e o tratamento que o sujeito dá ao real. Demonstra-se que o tratamento é um trabalho singular realizado pelo sujeito em parceria com os interventores. A prática entre vários e a transferência são colocadas em evidência como dispositivos que favorecem esse trabalho

Cet article est à propos d'une expérience institutionnel de stage à l'Institut Médico Pédagogique Notre Dame de la Sagesse, connu comme Le Courtil. Le Courtil est une institution belge qui accueille des enfants et des jeunes en difficultéau niveau du lien social. À partir d'un cas clinique d'une fille de huit ans, on discute l'importance des objets pour l'invention et pour le traitement du réel donné par le sujet. On démontre que le traitement est un travail singulier réalisé par le sujet en partenariat avec des intervenants. La pratique à plusieurs et le transfert sont mis en avant comme dispositifs qui favorisent ce travail

This article is about an internship experience that took place at the Institut Médico Pédagogique Notre Dame de la Sagesse, known as Le Courtil. Le Courtil is a Belgian institution dedicated to the reception and treatment ofchildren and young people with social bond difficulties. Based on a clinical case of an eight-year-old girl, the importance of objects for the invention and treatment of the real given by the subject is discussed. It is shown that the treatment is a singular work carried out by the subject in partnership with intervening parties. The multi-person practice and the transfer are put forward as devices that favour this work

Este artículo trata de una experiencia de pasantía institucional en el Institut Médico Pédagogique Notre Dame de la Sagesse, también conocido como Le Courtil. Le Courtil es una institución belga que acoge a niños y jóvenes con impasses en el lazo social. A partir del caso clínico de una niña de ocho años, se discute la importancia de los objetos para la invención y el tratamiento que el sujeto hace de lo real. Se demuestra que el tratamiento es un trabajo único realizado por el sujeto en compañía de los interventores. La práctica entre varios y la transferencia se destacan como dispositivos que favorecen este trabajo

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