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BACKGROUND: The use of technologies has had a significant impact on patient safety and the quality of care and has increased globally. In the literature, it has been reported that people die annually due to adverse events (AEs), and various methods exist for investigating and measuring AEs. However, some methods have a limited scope, data extraction, and the need for data standardization. In Brazil, there are few studies on the application of trigger tools, and this study is the first to create automated triggers in ambulatory care. OBJECTIVE: This study aims to develop a machine learning (ML)-based automated trigger for outpatient health care settings in Brazil. METHODS: A mixed methods research will be conducted within a design thinking framework and the principles will be applied in creating the automated triggers, following the stages of (1) empathize and define the problem, involving observations and inquiries to comprehend both the user and the challenge at hand; (2) ideation, where various solutions to the problem are generated; (3) prototyping, involving the construction of a minimal representation of the best solutions; (4) testing, where user feedback is obtained to refine the solution; and (5) implementation, where the refined solution is tested, changes are assessed, and scaling is considered. Furthermore, ML methods will be adopted to develop automated triggers, tailored to the local context in collaboration with an expert in the field. RESULTS: This protocol describes a research study in its preliminary stages, prior to any data gathering and analysis. The study was approved by the members of the organizations within the institution in January 2024 and by the ethics board of the University of São Paulo and the institution where the study will take place. in May 2024. As of June 2024, stage 1 commenced with data gathering for qualitative research. A separate paper focused on explaining the method of ML will be considered after the outcomes of stages 1 and 2 in this study. CONCLUSIONS: After the development of automated triggers in the outpatient setting, it will be possible to prevent and identify potential risks of AEs more promptly, providing valuable information. This technological innovation not only promotes advances in clinical practice but also contributes to the dissemination of techniques and knowledge related to patient safety. Additionally, health care professionals can adopt evidence-based preventive measures, reducing costs associated with AEs and hospital readmissions, enhancing productivity in outpatient care, and contributing to the safety, quality, and effectiveness of care provided. Additionally, in the future, if the outcome is successful, there is the potential to apply it in all units, as planned by the institutional organization. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): PRR1-10.2196/55466.

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BACKGROUND: To explore the value of high-resolution computed tomography (HRCT) in the differential diagnosis of benign and malignant ground-glass nodules (GGNs), and to provide a theoretical basis for the clinical application of HRCT. METHODS: A total of 208 patients with GGN who had been clinically confirmed by surgical pathology and clinical confirmation were collected, and HRCT target scanning technology was used to scan and collect general information of patients, and observe the distribution of GGN, GGN size, GGN cross-sectional area, diameter, transverse diameter, solid composition, relationship with bronchi, and relationship with blood vessels and other indicators. Multivariate regression analysis and risk factor prediction are performed. RESULTS: The differences were statistically significant in multivariate regression analysis, such as nodule location, maximum diameter, maximum cross-sectional area, GGN status, nodule boundary and relationship with blood vessels (P < 0.05). The results of ROC curve showed that the AUC value of nodule site and nodule boundary was greater than 0.5, and the nodule boundary AUC value was 0.676, which was more sensitive to predict whether GGN deteriorated to lung adenocarcinoma (LUAD). CONCLUSION: Nodule site and nodule boundary are effective risk predictors for LUAD in patients with GGN, and nodule boundary is the most valuable independent predictor.

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Endophytic fungi have been recognized as a valuable source for the production of biologically active compounds with potential applications in various domains. This study aimed to isolate endophytic fungi from Ampelopsis japonica (Thunb.) Makino and assess their anti-MRSA activity. Meanwhile, chromatographic separation techniques were applied to analyze the constituents of endophytic fungal secondary metabolites. The isolate BLR24, which exhibited strong inhibition activity against MRSA, was identified as Trichoderma virens based on morphological characteristics and ITS sequence analyses. The ethyl acetate extract of BLR24 (EA-BLR24) showed good anti-MRSA activity with the MIC and MBC values of 25 µg/mL and 50 µg/mL, separately. The inhibition of biofilm formation was up to 34.67% under MIC concentration treatment. Meanwhile, EA-BLR24 could significantly reduce the expression of biofilm-related genes (icaA, sarA, and agrA) of MRSA. Based on LC-MS/MS analysis, twenty compounds in EA-BLR24 could be annotated using the GNPS platform, mainly diketopiperazines. The anti-MRSA compound (Fr.1.1) was obtained from EA-BLR24 by bioassay-guided fractionation and determined as gliotoxin. The results indicated that endophytic Trichoderma virens BLR24 isolated from the medical plant A. japonica roots could be a promising source of natural anti-MRSA agents. Endophytic fungal secondary metabolites are abundant in biologically active compounds. Endophytic fungi from medicinal plants could be a source yielding bioactive metabolites of pharmaceutical importance.

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Abstract Objective: Chronic Rhinosinusitis with Polyps (CRSwNP) is characterized by high heterogeneity and postoperative recurrence rate. This study aims to explore the clinical significance of tissue Leukocyte-Specific Transcript 1 (LST1) in predicting CRSwNP recurrence. Methods: We enrolled 62 CRSwNP patients including 30 primary CRSwNP and 32 recurrent CRSwNP patients, and 40 Healthy Controls (HC). Tissue samples were collected. Tissue LST1 expression was assessed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Western Blotting (WB) and Immunofluorescence (IF) staining. The predictive values of LST1 expression for CRSwNP postoperative recurrence were assessed through the Receiver Operating Characteristic (ROC) curves. Results: The tissue levels of LST1 were significantly increased in the CRSwNP group than the HC group, especially in the recurrent group, and the elevated LST1 mRNA levels were positively correlated with the peripheral eosinophil percentages, tissue eosinophil counts and percentages. IF staining results showed that the LST1 protein levels were higher in CRSwNP patients, especially in the recurrent patients than in the HC group. ROC curves highlighted that tissue LST1 levels were associated with recurrent CRSwNP and exhibited a higher predictive ability for postoperative CRSwNP recurrence. Conclusion: This was the first report suggesting that LST1 expression was upregulated and associated with mucosal eosinophil infiltration and CRSwNP recurrence. Tissue LST1 could be a promising biomarker for predicting postoperative recurrence in CRwNP patients.

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OBJECTIVE: Chronic Rhinosinusitis with Polyps (CRSwNP) is characterized by high heterogeneity and postoperative recurrence rate. This study aims to explore the clinical significance of tissue Leukocyte-Specific Transcript 1 (LST1) in predicting CRSwNP recurrence. METHODS: We enrolled 62 CRSwNP patients including 30 primary CRSwNP and 32 recurrent CRSwNP patients, and 40 Healthy Controls (HC). Tissue samples were collected. Tissue LST1 expression was assessed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Western Blotting (WB) and Immunofluorescence (IF) staining. The predictive values of LST1 expression for CRSwNP postoperative recurrence were assessed through the Receiver Operating Characteristic (ROC) curves. RESULTS: The tissue levels of LST1 were significantly increased in the CRSwNP group than the HC group, especially in the recurrent group, and the elevated LST1 mRNA levels were positively correlated with the peripheral eosinophil percentages, tissue eosinophil counts and percentages. IF staining results showed that the LST1 protein levels were higher in CRSwNP patients, especially in the recurrent patients than in the HC group. ROC curves highlighted that tissue LST1 levels were associated with recurrent CRSwNP and exhibited a higher predictive ability for postoperative CRSwNP recurrence. CONCLUSION: This was the first report suggesting that LST1 expression was upregulated and associated with mucosal eosinophil infiltration and CRSwNP recurrence. Tissue LST1 could be a promising biomarker for predicting postoperative recurrence in CRwNP patients. LEVEL OF EVIDENCE: Level 5.

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PREDICT is a tool designed to estimate the benefits of adjuvant therapy and the overall survival of women with early breast cancer. The model uses clinical, histological, and immunohistochemical variables. This study aimed to evaluate the model's performance in a Brazilian population. We assessed the discrimination and calibration of the PREDICT model to estimate overall survival (OS) in five and ten years of follow-up in a cohort of 873 women with early breast cancer diagnosed from January 2001 to December 2016. A total of 743 patients had estrogen receptor (ER)-positive and 130 had ER-negative tumors. The area under the receiver operating characteristic (ROC) curve (AUC) for discrimination was 0.72 (95%CI: 0.66-0.78) at five years and 0.67 (95%CI: 0.61-0.72) at ten years for women with ER-positive tumors. The AUC was 0.72 (95%CI: 0.62-0.81) at five years and 0.67 (95%CI: 0.54-0.77) at ten years for women with ER-negative tumors. The predicted survival in ER-positive tumors was 91.0% (95%CI: 90.2-91.6%) at five years and 79.3% (95%CI: 77.7-81.0%) at ten years, and the observed survival 90.7% (95%CI: 88.6-92.9%) and 77.2% (95%CI: 73.4-81.4%), respectively. The predicted survival in ER-negative tumors was 84.5% (95%CI: 82.5-86.6%) at five years and 75.0% (95%CI: 71.6-78.5%) at ten years, and the observed survival 76.3% (95%CI: 69.1-84.3%) and 67.9% (95%CI: 58.6-78.6%), respectively. In conclusion, PREDICT was accurate to estimate OS in women with ER-positive tumors and overestimated the OS in women with ER-negative tumors.

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Introduction: Senna spectabilis is a multipurpose pantropical tree, used in agroforestry systems. Objective: To determine pod production (Pp) and their relationship with dasometric variables in S. spectabilis in the tropical dry forest. Methods: From August 2016 to February 2017, thirty trees in production stage were randomly selected. The random selection was formed of the more isolated trees from the total dispersion. The trees were monitored at the beginning and end of the study period, to determine dasometric measurements such as total height (Th), height to the first branch (Hb), crown height (Ch), Stem diameter (at 0.2 m height from the ground) (Db), crown diameter (Cd), and crown volume (Cv) measured. Pods were harvested by the researcher with cutting and height cutting tongs when their color began to change. Pearson correlations and univariate and multivariate regression analyses were performed between the dasometric variables and pod production. The potential number of trees/ha (NPa) was calculated by determining the occlusion percentage (Op) and the shadow area/tree (Ca); to estimate the production potential of fruits/ha, the production of fruits/tree was multiplied by (NPa). Results: Th was 6.16 ± 1.23 m, Hb 2.75 ± 0.52 m, Ch 3.41 ± 0.98 m, Db 20.43 ± 4.80 cm, Cd 7.46 ± 1.20 m and Cv 108.43 ± 61.38 m3/tree. There was a significant positive correlation between Hb, Cd, Db, with Pp of 0.592**, 0.592**, and 0.446* respectively. Pp was 32.73 ± 16.13 kg/tree and the dry matter production (MSP) was 17.84 ± 8.80 kg/tree. The result of the multivariate regression indicated that the second-order polynomial model presented best goodness of fit. Op was 73.4 7.92 %, the cup area was 49.3 m2/tree, Ca was 36.2 m2/tree, and NPa was 83 trees. Conclusions: The production of fresh pods/ tree in the S. spectabilis presents a potential in its availability as feed for ruminant or seed production. The potential production of pods in silvopastoral with S. spectabilis could be 2.72 t/ ha, and 1.64 t/ ha of dry pods, this shows the importance of trees and of pods production and nutritional contribution obtained for dry ecosystems.

Introducción: Senna spectabilis es un árbol pantropical multipropósito, utilizado en sistemas agroforestales. Objetivo: Determinar la producción de vainas (Pv) y la relación con las variables dasométricas en S. spectabilis en el bosque seco tropical. El número potencial de árboles/ha (NPa) fue calculado determinando el porcentaje de oclusión (Po) y el área de sombra/árbol (As); para calcular la producción potencial de frutos/ha, la producción de frutos/árbol fue multiplicada por (NPa). Métodos: Desde agosto del 2016 hasta febrero de 2017, treinta árboles en etapa de producción fueron seleccionados al azar, los más aislados del total de árboles dispersos fueron seleccionados, y fueron monitoreados al inicio y al final del período de estudio, para determinar las mediciones dasométricas como la altura total (At), altura a la primera rama (Apr), altura de la copa (Ac), diámetro del tallo (a 0.2 m altura desde el suelo) (Dt), diámetro de la copa (Dc) y volumen de copa (Vc). Las vainas se cosecharon cuando su color comenzó a cambiar. Se realizaron correlaciones de Pearson y análisis de regresión univariada y multivariada entre las variables dasométricas y la producción de vainas. El número potencial de árboles/ha (NPa) se calculó determinando el porcentaje de oclusión (Po) y el área de sombra/árbol (Asa); para estimar el potencial de producción de las vainas/ha, la producción de vainas/árbol se multiplicó por NPa. Resultados: La At fue de 6.16 ± 1.23 m, Apr 2.75 ± 0.52 m, Ac 3.41 ± 0.98 m, Db 20.43 ± 4.80 cm, Dc 7.46 ± 1.20 m y Vc 108.43 ± 61.38 m3/árbol. Existió una correlación positiva significativa entre Apr, Dc, Db, Pv de 0.592**, 0.592 ** y 0.446 * respectivamente. La Pv fue de 32.73 ± 16.13 kg y la producción de materia seca (PMS) fue de 17.84 ± 8.80 kg/árbol. El resultado de la regresión multivariada indicó que el modelo polinomial de segundo orden presentó la mejor bondad de ajuste. El Po de los árboles fue de 73.4 % ± 7.92 %, el área de copa fue de 49.3 m2/árbol, el Asa fue de 36.2 m2/árbol, el NPa fue de 83 árboles. Conclusiones: La producción de vainas frescas/árbol en el S. spectabilis presenta un potencial en la disponibilidad de alimento para los rumiantes o la producción de semillas. El potencial de producción de vainas en u arreglos silvopastoriles podría ser de 2.72 t/ha, y 1.64 t/ha de vainas secas, esto muestra la importancia del árbol de producción de vainas y la contribución nutricional para los ecosistemas secos.

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OBJECTIVES: Antineutrophil cyto plasmic antibody-associated vasculitis (AAV) is a fatal disease. Currently, predictors of mortality due to AAV are based on the distribution of organ involvement. The novel fibrosis index (NFI) is an index composed of laboratory results that reflect the degree of liver fibrosis. This study aimed to evaluate whether NFI can predict poor outcomes in patients with AAV without substantial liver disease. METHODS: A total of 210 patients with immunosuppressive drug-naïve AAV were retrospectively reviewed. NFI was calculated as follows: NFI=(serum bilirubin × (alkaline phosphatase)2)/(platelet count×(serum albumin)2). NFI cut-off was set at 1.24 (the highest quartile). Poor outcomes were defined as all-cause mortality, relapse, and end-stage renal disease (ESRD). RESULTS: During the median 34.5 months of follow-up, 21 patients (10%) died, 72 patients (34.3%) relapsed, and 38 patients (18.1%) had ESRD due to AAV progression. The median calculated NFI was 0.61, and it was higher in AAV patients with all-cause mortality than in those without mortality, but the difference was not statistically significant (1.26 vs. 0.59). AAV patients with NFI at diagnosis ≥1.24 exhibited a significantly lower cumulative patient survival rate than those with NFI at diagnosis <1.24 (p=0.002). Multivariate Cox hazard model analysis showed that NFI at diagnosis ≥1.24 was an independent predictor of all-cause mortality in AAV (hazard ratios [HR] 2.850, 95% confidence interval [CI] 1.026, 7.910). CONCLUSIONS: NFI ≥1.24, which may be an independent predictive marker for all-cause mortality in AAV patients without substantial liver disease.

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ABSTRACT COVID-19 has raised worldwide concern as spiraling into a pandemic. Reports about comprehensive investigation of COVID-19 viremia are extremely scanty. Herein, we present four COVID-19 patients with positive SARS-CoV-2 nucleic acid test in blood, accounting for 12.12% of 33 detected cases. Rapid deterioration of these cases with septic shock, accompanying with lung CT images enlarged rapidly, decrease of blood oxygen, heart rate drop (with asynchrony of hypoxemia) accompanied with SARS-CoV-2 viremia. It indicates that massive replication and releasing into blood of SARS-CoV-2 and secondary inflammation storm may lead to injury of multiple organs and poor prognosis. So, positive COVID-19 nucleic acid test in blood may be a good forecasting marker of rapid deterioration of COVID-19 pneumonia. In addition, clearance of viremia may indicate tendency for recovery.

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COVID-19 has raised worldwide concern as spiraling into a pandemic. Reports about comprehensive investigation of COVID-19 viremia are extremely scanty. Herein, we present four COVID-19 patients with positive SARS-CoV-2 nucleic acid test in blood, accounting for 12.12% of 33 detected cases. Rapid deterioration of these cases with septic shock, accompanying with lung CT images enlarged rapidly, decrease of blood oxygen, heart rate drop (with asynchrony of hypoxemia) accompanied with SARS-CoV-2 viremia. It indicates that massive replication and releasing into blood of SARS-CoV-2 and secondary inflammation storm may lead to injury of multiple organs and poor prognosis. So, positive COVID-19 nucleic acid test in blood may be a good forecasting marker of rapid deterioration of COVID-19 pneumonia. In addition, clearance of viremia may indicate tendency for recovery.

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Las Reacciones Adversas a Medicamentos (RAM) pueden causar incapacidad temporal o permanente al paciente, incluso tener un desenlace fatal. La ocurrencia de las RAM, presentan costos directos teniendo en cuenta: salario de la persona, gasto de material y costo de los medicamentos, los cuales aumentan la cuantía al tratarla. Con el objetivo de predecir que RAM le puede causar a un paciente, teniendo en cuenta: las características de los pacientes, la interacción entre medicamentos y las propias RAM; se crean y agrupan algunas técnicas de inteligencia artificial, donde cada una de ellas resuelve un problema determinado, pero en conjunto logran predecir una Reacción Adversa a Medicamentos. Entre las técnicas aplicadas se encuentra: razonamiento basado en caso, razonamiento basado en reglas y reconocimiento de patrones. La validación de cada una de estas técnicas se realiza de forma independiente, demostrando que el uso de estas, permite a los profesionales de salud contar con un apoyo informático en el momento de la consulta médica(AU)

Adverse Drug Reactions (ADRs) can cause temporary or permanent disability to the patient, even resulting in a fatal outcome. The occurrence of ADRs present direct costs taking into account: salary of the person, expenditure of material and cost of medicines, which increase the amount when dealing with it. With the aim of predicting which RAM can cause a patient, taking into account: the characteristics of patients, the interaction between medications and the RAM itself; Some artificial intelligence techniques are created and grouped, where each one solves a specific problem, but together they can predict an Adverse Drug Reaction. Among the applied techniques is: case-based reasoning, rule-based reasoning, and pattern recognition. The validation of each of these techniques is performed independently, demonstrating that the use of these, allows health professionals to have a computer support at the time of medical consultation(AU)

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Objetivos: evaluar el Índice de Pronóstico Inflamatorio Nutricional (PINI) mediante la determinación de Albúmina, Prealbúmina, Alfa-1-Glicoproteína y Proteína C Reactiva como pronóstico de riesgo durante la rehabilitación nutricional de niños desnutridos graves. Métodos: se estudiaron 36 niños(as) desnutridos infectados hospitalizados en el CRIN y 30 niños(as) controles entre 6 meses y 5 años con valoración antropométrica y nutricional. Se determinaron parámetros hematológicos y proteínas nutricionales e inflamatorias por turbi-dimetría. Los resultados se expresaron en términos de media ± desviación estándar con análisis de varianza en una sola dirección (ANO-VA) y la t de Student. Con 95% de confiabilidad estadística (p < 0,05) con el paquete estadístico STATA v. 10. Resultados: la antropometría mostró diferencia significativa en todos los parámetros comparados con el grupo control y umbral de referencia del National Center for Health Statistics. Parámetros hematológicos por debajo del límite inferior de la normalidad. Sesenta y nueve por ciento con anemia, 19 niños presentaron déficit de transferrina, 25% leucocitosis, 8% eosinofilia, 14% neutrofilia y 14% parasitosis. Las concentraciones de albúmina y prealbúmina significativamente disminuidas, la Alfa-1-Glicoproteína Ácida y Proteína C Reactiva, significativamente superiores a los niños control (p<0,001). Veinticinco por ciento presentaron un PINI de riesgo débil, 42% riesgo moderado, 22% riesgo alto y 11% riesgo vital correlacionado con patologías asociadas. Los niños sanos, un PINI menor a 1. Conclusiones: el PINI como complemento de la clínica ayuda a valorar la gravedad inicial de la infección, a preverla y a seguir su evolución en la desnutrición infantil. Es un micrométodo simple, rápido que puede ser repetido regularmente.

Objectives: To evaluate the prognostic inflammatory and nutritional index (PINI) by determining Albumin, Prealbumin, Alpha-1-glyco-protein and C-reactive protein as a predictor of risk for nutritional rehabilitation of severely malnourished children. Methods: We studied 36 children (as) in hospitalized malnourished CRIN infected and 30 children (as) controls between 6 months and 5 years with anthropo-metric and nutritional assessment. Hematological parameters were determined, and nutritional and inflammatory proteins by turbidi-metry. The results were expressed as mean ± standard deviation with ANOVA one-way and Student's t test. 95% statistical confidence (P <0.05) with the statistical package STATA. 10. Results: Anthropometry showed a significant difference in all parameters compared with the control group and reference threshold of the National Center for Health Statistics. Hematologic parameters below the lower limit of normality. 69% with anemia, 19 were deficient transferrin, leukocytosis 25%, 8% eosinophilia, 14% neutrophils and 14% parasitized. The albumin and prealbumin concentrations significantly decreased, the Alpha-1-acid glycoprotein and C-reactive protein significantly hig-her than control children (p<0.001). 25% showed a weak risk PINI, 42% moderate risk, high risk 22% and 11% correlated with life-threa-tening comorbidities. Healthy children, a PINI less than 1. Conclusions: The PINI as a complement to the clinic helps to assess the initial severity of the infection, to foresee and monitor trends in child malnutrition. A micromethod is simple, fast as it can be repeated regularly.

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The characteristics of exfoliated vaginal cells and vulvar biometry following estrus synchronization via two injections of 5mg Lutalyse® administered 7 days apart were investigated with the aim of their possible use to predict estrus in six adult WAD does. Four adult WAD bucks recently passed as satisfactory potential breeders were also involved in the study. The animals were maintained on 12 percent crude protein concentrate, greens and fresh water ad libitum. All measurements in the does were taken at an interval of 24 hours for six days beginning with the day of 2nd Lutalyse® injection. The does were introduced to the bucks 48 hours after the 2nd dose of Lutalyse® and separated from them after the 6th day. The 72-96 and 96-120 hours vaginal smears of 5 does (i.e. 83.3 percent) were characteristic during the study. They were positive for sperm cells and showed sharp increase in the degree of clumping of exfoliated cells. During these periods also, the differences in the percentage of superficial cells (i.e. 77.4 +/- 1.05 and 56.4 +/- 0.77) over other epithelial cells (12.2 +/- 0.38 and 1.30 +/- 0.82) respectively were significant (P<0.05). The percentage leucocytes also varied during the study but increased sharply during 96 -120 hours. The result on vulvar biometry between 0-72 hours and the period during which mating occurred (i.e. 72-120 hours) was not significant (P>0.05). All does with vaginal smear positive for sperm cells were confirmed pregnant at day 60 following mating by ultrasonography. The results of this study show that two injections of 5mg Lutalyse® 7 days apart will produce fertile estrus in the WAD doe. In conclusion, a careful evaluation of 24 hourly exfoliated vaginal cells will enhance synchronized estrus detection in WAD goat and improve their reproductive efficiency.

Fueron investigadas en seis cabras WAD hembras adultas, las características de las células vaginales exfoliadas y la biometría vulvar, tras una sincronización de estros a través de dos inyecciones de 5 mg de Lutalyse ®, administrados cada 7 días, con el fin de hacer posible el uso y predecir el estro. También participaron en este estudio cuatro machos adultos WAD probados recientemente como potenciales reproductores. Los animales fueron alimentados con un concentrado de proteína cruda de 12 por ciento, pastos y agua ad libitum. Las medidas en las cabras fueron tomadas con un intervalo de 24 horas, durante 6 días, a contar de la segunda inyección Lutalyse ®. Después de 48 horas de la 2 dosis de Lutalyse ® las cabras fueron cruzadas, y separadas de los machos después del 6 día. Fue realizado el estudio en frotis vaginales de 72-96 horas y de 96-120 horas, en 5 hembras (83,3 por ciento). Estos frotis fueron positivos para las células espermáticas y mostraron fuerte aumento en el grado de aglutinación de células de descamación. Durante estos períodos, las diferencias en el porcentaje de células superficiales (77,4 +/- 1,05 y 56,4 +/- 0,77) sobre las células epiteliales de otros (12,2 +/- 0,38 y 1,30 +/- 0,82) fueron significativas (P <0,05). También varió el porcentaje de leucocitos durante el estudio, pero aumentó considerablemente durante el periodo de 96-120 horas. El resultado de biometría vulvar entre 0-72 horas y del período durante el cual se produjo el apareamiento (72-120 horas) no fue significativa (P> 0,05). Todas las hembras con frotis vaginal positivo para células espermáticas, se les confirmó la preñez por ecografía, a los 60 días posterior al apareamiento. Los resultados de este estudio mostraron que dos inyecciones de 5 mg Lutalyse ® con 7 días de diferencia produce estro fértil en la cabra WAD. En conclusión, una evaluación cuidadosa de las células vaginales exfoliadas a las 24 horas, mejorará la detección del estro en cabras WAD y su eficiencia...

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En el manejo de la Candidiasis Bucal, los hallazgos clínicos no suelen ser suficientes, para determinar su diagnóstico e instaurar un tratamiento, siendo necesaria la realización de pruebas microbiológicas que garanticen una respuesta a la terapéutica instaurada, evitándose así, la resistencia a ciertos medicamentos. En la actualidad existe gran cantidad de pruebas de laboratorio para llegar a un diagnostico diferencial entre especies de Càndida, por lo que este artículo tiene por objetivo profundizar cuales de estas pruebas poseen mayor evidencia clínica en la determinación de la Candidiasis Bucal. Se utilizó la revisión sistemática como metodología que proporciona una apreciación crítica de datos que de otra manera serían inmanejables, integrando de manera eficiente toda información valida, la cual nos aporte una base racional para tomar decisiones en el establecimiento de políticas de atención. Al analizar los diversos trabajos, se determinó que las pruebas de laboratorio existentes, poseen poca evidencia clínica acerca de su utilidad, recomendándose realizar estudios que cumplan con los criterios de sensibilidad, especificidad y valor predictivo que garanticen su validez clínica.

Oral mycosis management, the clinics reward’s its not be enough, to determine it’s diagnosis and restore a treatment. It is be necessary to make labs prubes microbiology that guarantied a response an adequate treatment, it to avoid its resistance to some medicament. Actually exist a lot of labs prubes to find a differential diagnosis between species of Candida, the objective of this article its to low about which one of the labs prubes have the greatest clinic evidence to determine Oral Candidiasis, its used systematic review as methodology to proportion a critic appreciation of information in other way it would be unmanageable, to integrate of efficiency way validity information, the which one proportionated us a base rational to make decisions to establishment attention politics . At analyzed the different works, it determine that the existent lab prubes, it isn’t clinic evidence of its utility, we recommended to make studies that perform with the criterions sensibility, specify and predictive value.