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Objective: To investigate and analyze the clinical observation of porcine collagen membrane + artificial bovine bone granules guided tissue regeneration (GTR) combined with autologous concentration of growth factors (CGF) in the treatment of severe periodontitis bone defect. Methods: A total of 94 patients with severe periodontitis bone defects admitted to Shanxi Bethune Hospital from January 2019 to January 2022 were included. They were divided into two groups by simple randomization method. Patients in the control group were treated with porcine collagen membrane + artificial bovine bone granules GTR, while those in the observation group were treated with autologous CGF on the basis of the control group. Before and after treatment, periodontal clinical indicators [sulcus bleeding index (SBI), gingival retreat index (GR), probing depth (PD), clinical attachment loss (CAL), alveolar bone height (AH)] and bone resorption markers [Osteoprotegerin (OPG), bone gla protein (BGP), Type-1 collagen N-terminal peptide (NTX)] were compared between the two groups, and the incidence of postoperative complications in the two groups was recorded. Results: The total efficacy of observation group was significantly higher than that of control group (p<0.05). Three months after surgery, the observation group had lower levels of SBI, PD, CAL and NTX while higher levels of GR, AH, OPG and BGP than the control group (p<0.05). There was no significant difference in complication rate between the two groups (p>0.05). Conclusion: Porcine collagen membrane + artificial bovine bone granules GTR combined with autologous CGF boasts various benefits in the treatment of severe periodontitis bone defects, such as improvement of clinical outcomes, amelioration of periodontal tissue and inhibition of bone resorption.

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Objective: To observe the long-term outcome and biocompatibility of the porcine collagen membrane (DermalGen) after xenotransplantation in vivo. Methods: Twenty Sprague Dawley rats were randomly divided into 2 groups ( n=10). DermalGen were implanted subcutaneously into the dorsum of rats in experimental group, and the rats in control group were treated with sham-operation. At 3, 7, and 15 days and 1, 3, 6, and 12 months after operation, the samples of experimental group were harvested and gross observation, histological observation, CD31 immunohistochemical staining, and transmission electron microscope observation were taken to observe the inflammatory reaction, angiogenesis, and collagen arrangement. The skin tissues of the control group at 12 months were observed and compared. Results: All incisions healed in experimental group, without obvious swelling and inflammatory reaction. The DermalGen was closely contact with the surrounding tissue without obvious rejection, and it was still legible at 12 months. Histological observation of experimental group showed that the infiltration of fibroblasts and inflammatory cells were seen at 7 days. More capillaries and fibroblast cells were seen and the inflammatory response gradually faded at 15 days and 1 month. There were abundant vessels and cells in the DermalGen at 3 months. The angiogenesis and fibroblasts decreased gradually, and the collagen started to format and margin blended simultaneously at 6 and 12 months. The inflammatory cells in experimental group at 15 days and 1 month were significantly more than that in control group ( P<0.05), and no significant difference was found at 12 months between experimental group and control group ( P>0.05). Immunohistochemical staining of experimental group showed that the angiogenesis changed obviously with the time, and the density of vessels decreased significantly at 12 months. Compared with control group, the possitive expressions of CD31 in experimental group at 15 days and 12 months after operation were significantly decreased ( P<0.05), and were significantly increased at 1 month ( P<0.05). Transmision electron microscope observation showed that the arrangement of collagen in grafted DermalGen had no obvious changed when compared with the DermalGen, and vascular endothelial cell, capillarypericytes and fibroblast cells could be seen inside. Conclusion: The DermalGen structure is stable after long-term xenotransplantation and with good tolerogenic property in vivo.

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Methods: Twenty Sprague Dawley rats were randomly divided into 2 groups ( n=10). DermalGen were implanted subcutaneously into the dorsum of rats in experimental group, and the rats in control group were treated with sham-operation. At 3, 7, and 15 days and 1, 3, 6, and 12 months after operation, the samples of experimental group were harvested and gross observation, histological observation, CD31 immunohistochemical staining, and transmission electron microscope observation were taken to observe the inflammatory reaction, angiogenesis, and collagen arrangement. The skin tissues of the control group at 12 months were observed and compared.

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INTRODUCTION: Disadvantages of the radial forearm free flap (RFFF) are related to the forearm donor site, which requires skin grafting for closure. Due to that matter, an alternative technique using porcine collagen membrane instead of skin grafting was considered and evaluated for its efficacy of donor defect closure. MATERIAL AND METHODS: Ten patients requiring reconstruction of oral & maxillofacial defect underwent the single-staged reconstruction with the RFFF. The flap was elevated and the porcine collagen membrane (Rapiderm(®)) was fixed at the donor site with tie-over dressing. After leaving it for at least 2 weeks, it was replaced with an iodine-coated furazine gauze for continuous healing. RESULTS: The patients did not develop any tendon exposure or infection. All forearms healed within 1 month. The mean graft size harvested was 27.75 ± 3.08 cm(2). A greater amount of granulation tissue was formed under the dressing gauze which resulted in a less conspicuous donor site. After 6 months the average scar dimension was 9.48 ± 1.07 cm. Appearance was excellent with good wrist motility and skin color. CONCLUSION: The use of the porcine collagen membrane in the donor site of the RFFF is an alternative approach with less morbidity compared to other techniques. Nevertheless, further studies with a larger number of patients are needed to validate the present findings.

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